Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	396,201	A→G	A222A (GCT→GCC)	unknown ←	Protein translocase subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	396,201	0	A	G	100.0%	250.3 / NA	90	A222A (GCT→GCC)	unknown	Protein translocase subunit SecD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/52);  total (38/52)

ACTTCGGAATCGGAAGGCACCATACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGAGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGACAGTGCGG  >  1/395956‑396445                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aCTTCGGAATCGGAAGGCACCATACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCtt                                                                                                                                                                                                                                                  <  1:132791/250‑1 (MQ=255)     cggaatcggaaGGCACCATACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCttcattc                                                                                                                                                                                                                                             <  1:174580/251‑1 (MQ=255)                 gCACCATACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCa                                                                                                                                                                                                                                 >  1:121591/1‑251 (MQ=255)                      aTACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACgg                                                                                                                                                                                                                                      <  1:601453/241‑1 (MQ=255)                        aCCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACgg                                                                                                                                                                                                                                      <  1:652596/239‑1 (MQ=255)                         ccACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGcc                                                                                                                                                                                                                         <  1:632103/251‑1 (MQ=255)                         ccACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGcc                                                                                                                                                                                                                         <  1:74483/251‑1 (MQ=255)                                cTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGa                                                                                                                                                                                                                   <  1:533894/250‑1 (MQ=255)                                cTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGAc                                                                                                                                                                                                                  <  1:806812/251‑1 (MQ=255)                                cTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGAc                                                                                                                                                                                                                  <  1:286704/251‑1 (MQ=255)                                    cGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAgg                                                                                                                                                                                                              <  1:594247/251‑1 (MQ=255)                                    cGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAgg                                                                                                                                                                                                              <  1:782526/251‑1 (MQ=255)                                           aaaTTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTtct                                                                                                                                                                                                        >  1:126625/1‑250 (MQ=255)                                                      tGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGa                                                                                                                                                                                                                   >  1:633987/1‑228 (MQ=255)                                                      tGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGaca                                                                                                                                                                                             >  1:171770/1‑250 (MQ=255)                                                        cATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCt                                                                                                                                                                                                                        >  1:424779/1‑221 (MQ=255)                                                        cATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAgg                                                                                                                                                                                                              <  1:172616/231‑1 (MQ=255)                                                        cATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTa                                                                                                                                                                                          >  1:619278/1‑251 (MQ=255)                                                                ataCGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATa                                                                                                                                                                                                                                         >  1:36319/1‑196 (MQ=255)                                                                  aCGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGAt                                                                                                                                                                                <  1:477297/251‑1 (MQ=255)                                                                  aCGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGAt                                                                                                                                                                                <  1:670068/251‑1 (MQ=255)                                                                   cGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGAtt                                                                                                                                                                               <  1:715726/251‑1 (MQ=255)                                                                     aaaTTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCg                                                                                                                                                                             >  1:736033/1‑251 (MQ=255)                                                                       aTTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGcc                                                                                                                                                                                                                         >  1:567887/1‑205 (MQ=255)                                                                       aTTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCggg                                                                                                                                                                           <  1:358165/251‑1 (MQ=255)                                                                            aGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAggg                                                                                                                                                                                                                    <  1:88956/205‑1 (MQ=255)                                                                                   ccGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGt                                                                                                                                                               <  1:342416/251‑1 (MQ=255)                                                                                         cACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAg                                                                                                                                                         <  1:600234/251‑1 (MQ=255)                                                                                                aaTTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTg                                                                                                                                                    <  1:80384/249‑1 (MQ=255)                                                                                                           cACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGAt                                                                                                                                                                                >  1:769894/1‑210 (MQ=255)                                                                                                           cACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAgt                                                                                                                                       <  1:421775/251‑1 (MQ=255)                                                                                                           cACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAgt                                                                                                                                       <  1:624712/251‑1 (MQ=255)                                                                                                           cACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAgt                                                                                                                                       <  1:361248/251‑1 (MQ=255)                                                                                                             cGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAgttg                                                                                                                                     <  1:773905/251‑1 (MQ=255)                                                                                                               ggCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGAt                                                                                                                                                                                                                              >  1:252249/1‑160 (MQ=255)                                                                                                               ggCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAgttgtt                                                                                                                                   >  1:743556/1‑251 (MQ=255)                                                                                                                   gtgtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAgg                                                                                                                                                                                                              <  1:743029/172‑1 (MQ=255)                                                                                                                   gtgtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTa                                                                                                                                                              >  1:548413/1‑220 (MQ=255)                                                                                                                     gtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGa                                                                                                                                                                                                                   <  1:160315/165‑1 (MQ=255)                                                                                                                     gtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGAc                                                                                                                                                                                                                  <  1:14366/166‑1 (MQ=255)                                                                                                                     gtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAg                                                                                                                                                                                  >  1:270073/1‑198 (MQ=255)                                                                                                                     gtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTAc                                                                                                                                                 >  1:185222/1‑231 (MQ=255)                                                                                                                     gtCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAAt                                                                                                                             >  1:52121/1‑251 (MQ=255)                                                                                                                      tCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAAt                                                                                                                             <  1:292724/250‑1 (MQ=255)                                                                                                                       cTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGTAAAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATAc                                                                                                                                                                                                                                        >  1:544848/1‑143 (MQ=255)                                                                                                                       cTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTg                                                                                                                           >  1:390626/1‑251 (MQ=255)                                                                                                                            aCCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCCGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTaa                                                                                                                      >  1:473306/1‑251 (MQ=255)                                                                                                                              ccccGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAgg                                                                                                                    <  1:212620/251‑1 (MQ=255)                                                                                                                               ccccgTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTATGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGt                                                                                                                   <  1:454183/247‑1 (MQ=255)                                                                                                                                     aaTTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGGTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGGAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGc                                                                                                              >  1:472019/1‑250 (MQ=255)                                                                                                                                       ttCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATa                                                                                                                                                                                                                             <  1:161392/137‑1 (MQ=255)                                                                                                                                       ttCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGAtg                                                                                                                                                                                                  >  1:672799/1‑164 (MQ=255)                                                                                                                                           aCCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAggg                                                                                                                                                                                                                    >  1:234033/1‑142 (MQ=255)                                                                                                                                            ccACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGAt                                                                                                                                                                                                                              >  1:40666/1‑131 (MQ=255)                                                                                                                                               caATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGaaa                                                                                                   <  1:279237/251‑1 (MQ=255)                                                                                                                                                        ccGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATg                                                                                          >  1:661992/1‑251 (MQ=255)                                                                                                                                                                tGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAAt                                                                                  <  1:852984/251‑1 (MQ=255)                                                                                                                                                                  aCGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATgg                                                                                <  1:312598/251‑1 (MQ=255)                                                                                                                                                                  aCGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATgg                                                                                <  1:197936/251‑1 (MQ=255)                                                                                                                                                                       gAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGAt                                                                                                                                                                                <  1:195339/150‑1 (MQ=255)                                                                                                                                                                       gAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGgcg                                                                              >  1:96861/1‑248 (MQ=255)                                                                                                                                                                          tAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTTATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTAt                                                                        <  1:453199/251‑1 (MQ=255)                                                                                                                                                                          tAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTAt                                                                        <  1:86194/251‑1 (MQ=255)                                                                                                                                                                              ggCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGAtg                                                                                                                                                                                                  <  1:532261/125‑1 (MQ=255)                                                                                                                                                                                cTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   <  1:789692/250‑1 (MQ=255)                                                                                                                                                                                cTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   <  1:398815/250‑1 (MQ=255)                                                                                                                                                                                cTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   <  1:743613/250‑1 (MQ=255)                                                                                                                                                                                cTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGAGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   <  1:603050/250‑1 (MQ=255)                                                                                                                                                                                      cGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTc                                                             <  1:275418/250‑1 (MQ=255)                                                                                                                                                                                      cGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTc                                                             <  1:493403/250‑1 (MQ=255)                                                                                                                                                                                          gCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTg                                                         >  1:376273/1‑250 (MQ=255)                                                                                                                                                                                             cAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAg                                                                                                                                                                                  >  1:169585/1‑126 (MQ=255)                                                                                                                                                                                               aTTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGatat                                                                  <  1:402342/236‑1 (MQ=255)                                                                                                                                                                                                  cGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATgg                                                                                <  1:759101/219‑1 (MQ=255)                                                                                                                                                                                                      aaCACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTAc                                            <  1:417098/251‑1 (MQ=255)                                                                                                                                                                                                        cacGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGatat                                                                  <  1:843507/227‑1 (MQ=255)                                                                                                                                                                                                          cGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCttcat                                                                                                                                                                                                                                               >  1:385667/1‑52 (MQ=255)                                                                                                                                                                                                              tACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGaa                                    >  1:791794/1‑251 (MQ=255)                                                                                                                                                                                                               aCGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAAt                                   <  1:240527/251‑1 (MQ=255)                                                                                                                                                                                                               aCGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGATTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   >  1:450625/1‑219 (MQ=255)                                                                                                                                                                                                                  aaaaaaTACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATc                                  >  1:433598/3‑250 (MQ=255)                                                                                                                                                                                                                       aTACTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCaa                            >  1:614884/1‑251 (MQ=255)                                                                                                                                                                                                                          cTCAGGTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   >  1:543222/1‑209 (MQ=255)                                                                                                                                                                                                                               gTTTTGCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGata                                                                   <  1:22759/204‑1 (MQ=255)                                                                                                                                                                                                                                    gCTCAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGt               >  1:481819/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAATAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGAc                 >  1:188618/1‑246 (MQ=255)                                                                                                                                                                                                                                          tAGCGCTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGAc         <  1:685378/251‑1 (MQ=255)                                                                                                                                                                                                                                              gcTCTCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGACAGTg     >  1:50742/1‑251 (MQ=255)                                                                                                                                                                                                                                               ctctCACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGACAGTg     >  1:773415/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ctcACGGGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGACAGTGCgg  >  1:881443/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ACTTCGGAATCGGAAGGCACCATACCACGCGCTGCCGCTTCTAAATTAGCGCTTGCATTAACTATACGAAATTCAAGGGTAGCCGTTGCACCGAGAATTTCTTTCGCACGGGCAGTGTCTTGTACCCCCGGTAATTCAACCACAATGCGTTCCGCCCCTTGACGCTGAATAACGGCTTCGGCGACGCCCAATTCGGTAACACGTTTACGT‑AAAATACTCAGGTTTTGCTCAATAGCGCTCTCACGAGCTTCATTCAATACGGAATCAGATAAGCCTAGGGACAAGGTGTTCTCGGATGTGACACTAACATCTAGATTCGGGTGCTGTTTGCGTAAGTAGCGTTGTACGTCAGAAAGTTGTTCAGAATTGGTTAAGGTTACGCCGGTGCTGAAATTATCAATGGATTTAATGGCGCTGTATTGATATTTTTCTTTGCGCAGTTCGGTACGCAAAGAATCCTGCAACTGTTCCTGACGTTTGGACAGTGCGG  >  1/395956‑396445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑