Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	55,573	A→G	Q213R (CAG→CGG)	unknown →	Signal recognition particle receptor FtsY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	55,573	0	A	G	100.0%	217.0 / NA	79	Q213R (CAG→CGG)	unknown	Signal recognition particle receptor FtsY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/30);  total (49/30)

GACATGGTGGAAACGCAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGGAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACAGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGTCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAGCAGTT  >  3/55338‑55816                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    gACATGGTGGAAACGCAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGa                                                                                                                                                                                                                                      >  1:876467/1‑251 (MQ=255)                cAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:552112/250‑1 (MQ=255)                cAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:632480/250‑1 (MQ=255)                cAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:856948/250‑1 (MQ=255)                cAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:775539/250‑1 (MQ=255)                       aaCCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:105764/1‑243 (MQ=255)                              tGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGaaa                                                                                                                                                                                                         <  1:704911/251‑1 (MQ=255)                                          tttCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATcc                                                                                                                                                                                             <  1:824868/251‑1 (MQ=255)                                                   cTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCgg                                                                                                                                                                                    >  1:106050/1‑251 (MQ=255)                                                   cTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCgg                                                                                                                                                                                    >  1:676363/1‑251 (MQ=255)                                                     tattaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTg                                                                                                                                                                                                                               >  1:830576/1‑206 (MQ=255)                                                     tattaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:752616/1‑213 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCaa                                                                                                                                                                                                                                              >  1:763172/1‑190 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCaa                                                                                                                                                                                                                                              >  1:240943/1‑190 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATc                                                                                                                                                                                                                           <  1:872133/209‑1 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGc                                                                                                                                                                                                                     <  1:280077/215‑1 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAAt                                                                                                                                                                                                               <  1:488/221‑1 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTgg                                                                                                                                                                                 >  1:550224/1‑251 (MQ=255)                                                      attaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACACTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTgg                                                                                                                                                                                 >  1:430142/1‑251 (MQ=255)                                                       ttaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCCCCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTgg                                                                                                                                                                                 >  1:570424/1‑250 (MQ=255)                                                       ttaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:806973/1‑211 (MQ=255)                                                       ttaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAAc                                                                                                                                                                                       >  1:668676/1‑244 (MQ=255)                                                       ttaAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTgg                                                                                                                                                                                 >  1:893847/1‑250 (MQ=255)                                                               cTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCg                                                                                                                                                                         <  1:757657/250‑1 (MQ=255)                                                                  ggTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:778213/200‑1 (MQ=255)                                                                  ggTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTg                                                                                                                                                                      >  1:496194/1‑250 (MQ=255)                                                                   gTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGca                                                                                                                                                                    >  1:490935/1‑251 (MQ=255)                                                                          ccAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATcc                                                                                                                                                                                             >  1:247039/1‑219 (MQ=255)                                                                               caaaATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:665772/1‑187 (MQ=255)                                                                                 aaaTATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTACAATTGGACATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGCCGgcagca                                                                                                                                                      >  1:764647/1‑251 (MQ=255)                                                                                       cGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAgc                                                                                                                                                <  1:872722/251‑2 (MQ=255)                                                                                         gTGCGGGTTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        <  1:429351/177‑1 (MQ=255)                                                                                         gTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:877814/1‑177 (MQ=255)                                                                                         gTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAAt                                                                                                                                                                                                        <  1:526125/193‑1 (MQ=255)                                                                                              ggCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTAtgt                                                                                                                                         >  1:779803/1‑251 (MQ=255)                                                                                                  tCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGAtt                                                                                                                                     >  1:632080/1‑251 (MQ=255)                                                                                                       aGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAACATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGAt                                                                                                                                >  1:426345/1‑251 (MQ=255)                                                                                                        gCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCaa                                                                                                                                                                                                                                              >  1:134701/1‑140 (MQ=255)                                                                                                        gCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGcac                                                                                                                                                                   <  1:387358/215‑1 (MQ=255)                                                                                                        gCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGAt                                                                                                                                >  1:672791/1‑250 (MQ=255)                                                                                                        gCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCACCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGAtg                                                                                                                               >  1:643598/1‑251 (MQ=255)                                                                                                         cTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGAtgg                                                                                                                              <  1:723366/251‑1 (MQ=255)                                                                                                         cTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGAt                                                                                                                                <  1:255413/249‑1 (MQ=255)                                                                                                             ttCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTggg                                                                                                                          >  1:318754/1‑251 (MQ=255)                                                                                                                     cAAAAAAATCGACGACGACTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAAc                                                                                                                   <  1:771858/250‑1 (MQ=255)                                                                                                                              cgacgacgaCTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCa                                                                                                         <  1:579872/251‑1 (MQ=255)                                                                                                                                  gacgacTTGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAgc                                                                                                                                                <  1:734618/208‑2 (MQ=255)                                                                                                                                        ttGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAaccaccac                                                                                               <  1:567379/251‑1 (MQ=255)                                                                                                                                        ttGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAaccaccac                                                                                               <  1:835283/251‑1 (MQ=255)                                                                                                                                         tGTTCGAGAAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAaccaccacc                                                                                              <  1:220938/251‑1 (MQ=255)                                                                                                                                                             cAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCaa                                                                          >  1:868300/1‑251 (MQ=255)                                                                                                                                                             cAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCaa                                                                          >  1:575040/1‑251 (MQ=255)                                                                                                                                                               gTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCAc                                                                                                                                                                                                                                 <  1:618704/98‑1 (MQ=255)                                                                                                                                                                       tCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATg                                                                <  1:572775/251‑1 (MQ=255)                                                                                                                                                                        cGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATgg                                                               <  1:230910/251‑1 (MQ=255)                                                                                                                                                                             aCATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGc                                                          <  1:387113/251‑1 (MQ=255)                                                                                                                                                                                       gCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGt                                                                                                                                                                        >  1:361187/1‑131 (MQ=255)                                                                                                                                                                                                          aTCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAAc                                                                                                                                                                                                                        >  1:58300/1‑64 (MQ=255)                                                                                                                                                                                                          aTCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATa                              >  1:487886/1‑250 (MQ=255)                                                                                                                                                                                                          aTCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATAc                             <  1:683599/251‑1 (MQ=255)                                                                                                                                                                                                           tCAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTAtg                                                                                                                                          >  1:797932/1‑141 (MQ=255)                                                                                                                                                                                                            cAATAATCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATAc                             >  1:697578/1‑249 (MQ=255)                                                                                                                                                                                                                 atCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCcgc                      <  1:130831/251‑1 (MQ=255)                                                                                                                                                                                                                 atCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCcg                       >  1:47608/1‑250 (MQ=255)                                                                                                                                                                                                                 atCTGACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCcg                       >  1:30489/1‑250 (MQ=255)                                                                                                                                                                                                                     gACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCgcgg                  >  1:674530/1‑251 (MQ=255)                                                                                                                                                                                                                     gACCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGC‑AAACCCCCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCgcgg                  >  1:798021/1‑250 (MQ=255)                                                                                                                                                                                                                      aCCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAAc                                                                                                                   >  1:345510/1‑153 (MQ=255)                                                                                                                                                                                                                      aCCCAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCgcgg                  >  1:813079/1‑250 (MQ=255)                                                                                                                                                                                                                        ccAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATg                                              >  1:473114/1‑220 (MQ=255)                                                                                                                                                                                                                         cAACACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGcggcgg               >  1:578293/1‑250 (MQ=255)                                                                                                                                                                                                                          aaCACGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCa             <  1:293258/251‑1 (MQ=255)                                                                                                                                                                                                                           acacGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAg            >  1:718760/1‑251 (MQ=255)                                                                                                                                                                                                                           acacGCTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAg            >  1:330337/1‑251 (MQ=255)                                                                                                                                                                                                                                cTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAg       >  1:758699/1‑251 (MQ=255)                                                                                                                                                                                                                                cTACCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAg       >  1:702622/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCCCGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAGCa     >  1:749936/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATAcc                            >  1:286269/1‑225 (MQ=255)                                                                                                                                                                                                                                     cGCCAACGGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGCCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAGCAGtt  >  1:335193/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    GACATGGTGGAAACGCAGGAAAAACCAAGTGAAGGCGGCTTTTTCAGTCGCTTATTAAAAGGCTTGGTGAAAACCAAACAAAATATCGGTGCGGGCTTCCGTAGCTTTTTCTTAGGCAAAAAAATCGACGACGACTTGTTCGAGGAATTGGAAGAACAGTTACTTATCGCCGACATCGGCGTGCCGACCACCACCAAAATTATCAATAATCTGACCCAACACGCTACCCGCCAACAGTTGCAAAATGCGGATTCACTGTATCAACAGCTAAAATTGGAAATGGGCGAAATCCTGAAACCGGTGGCGCAACCGTTGCACATCGACGGCAGCAAAAAGTCTTATGTGATTTTGATGGTGGGCGTAAACGGCGTGGGCAAAACCACCACCATCGGCAAGTTGGCGCGCAAATTCCAAATGGAAGGCAAATCCGTCATGTTGGCGGCGGGCGATACCTTCCGCGCGGCGGCAGTGGAGCAGTT  >  3/55338‑55816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑