Predicted mutation
evidence seq id position mutation annotation gene description
RA 3 38,883 G→A E117K (GAG→AAG)  unknown → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*338,8830GA100.0% 215.7 / NA 80E117K (GAG→AAG) unknownhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/43);  total (37/43)

GCTCGTGATTATGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTGAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATAGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGTTTATCTCTAATCCGAATGCTTGGATTGAAGCTGCA  >  3/38640‑39128
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
gCTCGTGATTATGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACg                                                                                                                                                                                                                                                >  1:828319/1‑251 (MQ=255)
     tGATTATGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAg                                                                                                                                                                                                                                           >  1:285473/1‑251 (MQ=255)
          aTGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAACAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGc                                                                                                                                                                                                                                                    >  1:438382/1‑237 (MQ=255)
          aTGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAAc                                                                                                                                                                                                                                      <  1:431630/251‑1 (MQ=255)
           tGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATa                                                                                                                                                                                                                                        <  1:172766/248‑1 (MQ=255)
                atatTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGaag                                                                                                                                                                                                                                 >  1:896960/1‑250 (MQ=255)
                atatTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGaag                                                                                                                                                                                                                                 >  1:793472/1‑250 (MQ=255)
                          aaataaatTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATa                                                                                                                                                                                                                                        >  1:845288/1‑233 (MQ=255)
                            ataaatTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCCTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGt                                                                                                                                                                                                                          <  1:316418/245‑1 (MQ=255)
                               aatTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAAtt                                                                                                                                                                                                                 >  1:504262/1‑251 (MQ=255)
                                      gCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTgg                                                                                                                                                                                                          >  1:754035/1‑251 (MQ=255)
                                      gCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTgg                                                                                                                                                                                                          >  1:619959/1‑251 (MQ=255)
                                           atgatATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGc                                                                                                                                                                                                                                                    <  1:107074/204‑1 (MQ=255)
                                           atgatATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGtt                                                                                                                                                                                                     >  1:590991/1‑251 (MQ=255)
                                              atatTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGc                                                                                                                                                                                                                     <  1:782884/232‑1 (MQ=255)
                                                    atatCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgat                                                                                                                                                                                            <  1:606961/251‑1 (MQ=255)
                                                     tatCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTAt                                                                                                                                                                                                             >  1:250691/1‑233 (MQ=255)
                                                      atCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatag                                                                                                                                                                                          <  1:737291/251‑1 (MQ=255)
                                                      atCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatag                                                                                                                                                                                          <  1:622675/251‑1 (MQ=255)
                                                          aTAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGatat                                                                                                                                                                                      >  1:777984/1‑251 (MQ=255)
                                                               ggggAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatag                                                                                                                                                                                          >  1:862699/1‑242 (MQ=255)
                                                                gggAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGa                                                                                                                                                                                                                                                  >  1:700153/1‑185 (MQ=255)
                                                                   aaaaTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGttatta                                                                                                                                                                              <  1:795388/250‑1 (MQ=255)
                                                                   aaaaTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTtattat                                                                                                                                                                             <  1:200967/251‑1 (MQ=255)
                                                                   aaaaTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTAACATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGttatta                                                                                                                                                                              <  1:370315/250‑1 (MQ=255)
                                                                    aaaTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATg                                                                                                                                                                            <  1:614547/251‑1 (MQ=255)
                                                                    aaaTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATg                                                                                                                                                                            <  1:306532/251‑1 (MQ=255)
                                                                      aTGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGAt                                                                                                                                                                          >  1:2390/1‑251 (MQ=255)
                                                                        gATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATa                                                                                                                                                                                                                                        <  1:752763/187‑1 (MQ=255)
                                                                                  gTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAAtt                                                                                                                                                                >  1:790961/1‑249 (MQ=255)
                                                                                        ccGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGc                                                                                                                                                        <  1:266121/251‑1 (MQ=255)
                                                                                           gCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTtattat                                                                                                                                                                             <  1:547946/227‑1 (MQ=255)
                                                                                            cTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCtttt                                                                                                                                                    >  1:880110/1‑251 (MQ=255)
                                                                                                        gaCTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatag                                                                                                                                                                                          <  1:264477/201‑1 (MQ=255)
                                                                                                                gTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTa                                                                                                                                                                    >  1:895119/1‑215 (MQ=255)
                                                                                                                                   gATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATa                                                                                                             >  1:496615/1‑251 (MQ=255)
                                                                                                                                       tttGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACAcc                                                                                                         <  1:813281/251‑1 (MQ=255)
                                                                                                                                               ttCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtact                                                                                                  <  1:33395/250‑1 (MQ=255)
                                                                                                                                               ttCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtac                                                                                                   <  1:716465/249‑1 (MQ=255)
                                                                                                                                                tCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATgg                                                                                                                                                         >  1:878092/1‑194 (MQ=255)
                                                                                                                                                tCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtactac                                                                                                <  1:82377/251‑1 (MQ=255)
                                                                                                                                                  cGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtac                                                                                                   <  1:720338/246‑1 (MQ=255)
                                                                                                                                                        cATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        <  1:774397/251‑1 (MQ=255)
                                                                                                                                                        cATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        <  1:377845/251‑1 (MQ=255)
                                                                                                                                                        cATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        <  1:681821/251‑1 (MQ=255)
                                                                                                                                                          ttGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatag                                                                                                                                                                                          <  1:401364/151‑1 (MQ=255)
                                                                                                                                                            gTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTg                                                                                                                                                                                                           <  1:495810/132‑1 (MQ=255)
                                                                                                                                                            gTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgatagat                                                                                                                                                                                        >  1:808277/1‑151 (MQ=255)
                                                                                                                                                            gTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTc                                                                                                                                                               >  1:34368/1‑176 (MQ=255)
                                                                                                                                                            gTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATac                                                                                                            <  1:785973/227‑1 (MQ=255)
                                                                                                                                                            gTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtac                                                                                                   >  1:830429/1‑236 (MQ=255)
                                                                                                                                                                 tATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTAt                                                                                                                                                                                                             >  1:705045/1‑125 (MQ=255)
                                                                                                                                                                 tATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATacac                                                                                                          <  1:497359/224‑1 (MQ=255)
                                                                                                                                                                  aTCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtac                                                                                                   >  1:338199/1‑230 (MQ=255)
                                                                                                                                                                             tataAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAAtt                                                                   <  1:322967/251‑1 (MQ=255)
                                                                                                                                                                                    aaTTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCatatat                                                            <  1:511625/251‑1 (MQ=255)
                                                                                                                                                                                      ttCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGc                                                          <  1:802729/251‑1 (MQ=255)
                                                                                                                                                                                                         gATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGAAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATCGGTCGTTCTACCTGATAGATATGGTTATTATGATAACGTAAATTCATATGGCt                                                                                                                                                       >  1:355880/1‑139 (MQ=255)
                                                                                                                                                                                                          aTCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGAt                                                                                                                                                                          <  1:350533/119‑1 (MQ=255)
                                                                                                                                                                                                          aTCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAg                                      >  1:504667/1‑251 (MQ=255)
                                                                                                                                                                                                           tCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGt                                     >  1:573053/1‑251 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGt                                                                                                                                                                                                         <  1:723211/84‑1 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGa                                                                                                                                                                           >  1:23947/1‑114 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtact                                                                                                  >  1:288992/1‑187 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        <  1:134652/197‑1 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAgg                                                                                   >  1:382421/1‑202 (MQ=255)
                                                                                                                                                                                                              aTGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGTTTa                                  >  1:498206/1‑251 (MQ=255)
                                                                                                                                                                                                               tGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTgat                                                                                                                                                                                            <  1:690052/96‑1 (MQ=255)
                                                                                                                                                                                                                     ccTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTc                                                                                                                                                               <  1:857937/119‑1 (MQ=255)
                                                                                                                                                                                                                     ccTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGata                                                                                                                                           >  1:396938/1‑139 (MQ=255)
                                                                                                                                                                                                                     ccTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATac                                                                                                            >  1:351519/1‑170 (MQ=255)
                                                                                                                                                                                                                     ccTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTg                                             <  1:541095/233‑1 (MQ=255)
                                                                                                                                                                                                                     ccTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGTTTAtctc                              <  1:227813/248‑1 (MQ=255)
                                                                                                                                                                                                                      cTTGGGAAATTGCTTATTCACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCAt                                                                                                              <  1:894773/167‑1 (MQ=255)
                                                                                                                                                                                                                                        cACTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtac                                                                                                   <  1:673429/160‑1 (MQ=255)
                                                                                                                                                                                                                                          cTAAGAAGTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        >  1:809653/1‑169 (MQ=255)
                                                                                                                                                                                                                                              gaagTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTc                                                                                                                                                               >  1:432003/1‑94 (MQ=255)
                                                                                                                                                                                                                                              gaagTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATAtact                                                                                                  <  1:364108/155‑1 (MQ=255)
                                                                                                                                                                                                                                              gaagTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAAc                                                                                        <  1:368293/165‑1 (MQ=255)
                                                                                                                                                                                                                                              gaagTAAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATGGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGTTTATCTCTAATCCGAATGCTTGGATTGAAGCTGCa  >  1:505853/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
GCTCGTGATTATGTTGATATTATTCAAAATAAATTTGAGCGTGATGATATTCATATCAATAAAGGGGAAAATGATAATGAAAGTCTTGCCGGCTTTAAAACGGAGACTATTCGTTCAAAATTAGCAGAAAAGATTTTTGATAGTTCCGTTACCATTGTTCTTATCTCCCCCAATATAAAAAATTCTTGGAAATCAGAGAAAGATCAATGGATACCTTGGGAAATTGCTTATTCACTAAGAAGTGAGCGACGTCCAGATAACCGAAGCAAGCGTAATGCAATTTTATTGGTCGTTCTACCTGATAGATATGGTTATTATGATTACGTAAATTCATATGGCTTTTATTTTGATATTATTCAAAGAAACTTGAATAACCTGCATACACCATATACTACATATAAACTAAGGAATAGTTGTTCTAATTCATATATGCTTCAATGTAACTGGGATAAGTTTATCTCTAATCCGAATGCTTGGATTGAAGCTGCA  >  3/38640‑39128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑