| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 3 | 30,670 | A→G | intergenic (+130/+189) | unknown → / ← unknown | S‑ribosylhomocysteine lyase (EC 4.4.1.21) @ Autoinducer‑2 production protein LuxS/LSU rRNA pseudouridine(746) synthase (EC 5.4.99.29) @ tRNA pseudouridine(32) synthase (EC 5.4.99.28) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 3 | 30,670 | 0 | A | G | 98.7% | 219.9 / ‑3.0 | 78 | intergenic (+130/+189) | unknown/unknown | S‑ribosylhomocysteine lyase (EC 4.4.1.21) @ Autoinducer‑2 production protein LuxS/LSU rRNA pseudouridine(746) synthase (EC 5.4.99.29) @ tRNA pseudouridine(32) synthase (EC 5.4.99.28) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (32/45); minor base . (1/0); total (33/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TACACCGAGCATTCTTTGGAAGAAGCACATCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGAAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGTTTCGCCGGTGATCGGGTGCCGGA > 3/30424‑30914 | tACACCGAGCATTCTTTGGAAGAAGCACATCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAgg < 1:307681/251‑1 (MQ=255) acacCGAGCATTCTTTGGAAGAAGCACATCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGc < 1:639714/251‑1 (MQ=255) tttGGAAGAAGCACATCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGa < 1:276421/251‑1 (MQ=255) acaTCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATaa < 1:29915/251‑1 (MQ=255) cAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGAc < 1:387230/251‑1 (MQ=255) cAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGAc < 1:365418/251‑1 (MQ=255) cGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACaataa < 1:118625/251‑1 (MQ=255) cGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACaataa < 1:44672/251‑1 (MQ=255) caAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTg > 1:190754/1‑250 (MQ=255) gtgtTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTgaaaaaga > 1:457669/1‑251 (MQ=255) ggATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGAc < 1:358736/231‑1 (MQ=255) cgGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACaataa > 1:703765/1‑230 (MQ=255) gTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGca > 1:555348/1‑251 (MQ=255) gTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGc < 1:854217/250‑1 (MQ=255) gTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTAAAAAAGAAAAATATTTGGca > 1:490262/1‑251 (MQ=255) cGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGcaatac < 1:47730/251‑1 (MQ=255) ataaaAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGa < 1:820506/251‑1 (MQ=255) taaaAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGAc < 1:159047/251‑1 (MQ=255) aTTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGc < 1:157549/249‑1 (MQ=255) aCCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATaa > 1:60140/1‑231 (MQ=255) aCCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGa > 1:328023/1‑234 (MQ=255) aaGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTg < 1:538209/251‑1 (MQ=255) taAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGAc < 1:112354/175‑1 (MQ=255) aCAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAgg > 1:514759/1‑169 (MQ=255) aCAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGc > 1:204968/1‑250 (MQ=255) cAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAg < 1:533982/153‑1 (MQ=255) cAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGc > 1:750635/1‑249 (MQ=255) aTAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCg > 1:151834/1‑251 (MQ=255) gagaCGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaa < 1:554495/250‑1 (MQ=255) aCGTTTATGACCGCCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAAGAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaaaga < 1:741267/250‑1 (MQ=255) cGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAAtctc > 1:803805/1‑137 (MQ=255) gTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGcaatac < 1:309779/190‑1 (MQ=255) gTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaaagaaaa > 1:82567/1‑251 (MQ=255) caccaTCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGaaaagaaaagag > 1:680910/1‑243 (MQ=255) ccaTCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGa > 1:700694/1‑251 (MQ=255) tCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGc > 1:288054/1‑223 (MQ=255) tCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaa < 1:241527/231‑1 (MQ=255) tCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAAtt > 1:467745/1‑251 (MQ=255) gTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGc > 1:814281/1‑112 (MQ=255) gTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGc < 1:254634/168‑1 (MQ=255) gTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCaa < 1:353565/188‑1 (MQ=255) gTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTAt > 1:299906/1‑217 (MQ=255) gTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaaagaaa < 1:460586/233‑1 (MQ=255) aCATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAgg < 1:305717/251‑1 (MQ=255) aCCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAAc < 1:606427/178‑1 (MQ=255) ccTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGttt > 1:291262/1‑250 (MQ=255) aaaGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACAt < 1:853607/251‑1 (MQ=255) aTTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGc > 1:153940/1‑167 (MQ=255) ggCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATc > 1:404397/1‑250 (MQ=255) ggATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATG‑GAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGgaaaagaaa > 1:635841/1‑191 (MQ=255) gATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTaa > 1:56609/1‑251 (MQ=255) tGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTACAAc < 1:876937/251‑1 (MQ=255) tGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAAc < 1:852660/251‑1 (MQ=255) tGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAAc < 1:438419/251‑1 (MQ=255) ccGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAAcc < 1:27250/250‑1 (MQ=255) ccGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAAcc < 1:453707/250‑1 (MQ=255) gTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGc > 1:607140/1‑135 (MQ=255) gTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGaaacgggttc < 1:186059/149‑9 (MQ=255) gTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGa > 1:300597/1‑250 (MQ=255) ccAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTgtggtg < 1:656232/251‑1 (MQ=255) ccAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTgtggtg < 1:342408/251‑1 (MQ=255) cAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGa < 1:870757/251‑1 (MQ=255) cAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGa < 1:255951/251‑1 (MQ=255) cAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGa < 1:767550/251‑1 (MQ=255) cTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGa > 1:681653/1‑111 (MQ=255) aGCCTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGa > 1:528239/1‑104 (MQ=255) ccTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATgg < 1:423401/248‑1 (MQ=255) ccTGTCCCCTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGt < 1:503497/249‑1 (MQ=255) cTTCCGGCCCGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGa < 1:176201/231‑1 (MQ=255) cccGCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGTTTCGCCGGTGATCggg < 1:557252/250‑1 (MQ=255) gCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATc > 1:777999/1‑187 (MQ=255) gCCATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAAt < 1:819932/223‑1 (MQ=255) ccATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAAc < 1:180776/204‑1 (MQ=255) ccATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGa < 1:687458/220‑1 (MQ=255) ccATCATGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGTTTCGCCGGTGATCGGGTGCCg > 1:364365/1‑251 (MQ=255) atcatGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTc > 1:598908/1‑159 (MQ=255) atcatGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGTTTCGCCGGTGATCGGGTGCCGGa > 1:639523/1‑251 (MQ=255) tcatGGAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAacac > 1:143943/1‑173 (MQ=255) | TACACCGAGCATTCTTTGGAAGAAGCACATCAAATCGCACAAAATGTGTTGGATCGCGGTATCGGCGTAAATAAAAATGAAGATTTAACCTTAGACGAAAGCTTATTAAAACAATAAGGAGACGTTTATGACCACCATCGGTACATAACCTGAAAGCATTCGTCATTTTTTAGTGGCAGAAGGATTCGGATTGCCGGTTCTAATCGCCAACTTCATCAGCCTGTCCCCTTCCGGCCCGCCATCATGAAAGGCAAATCTCAAAAGATCGGAATGATAAGGACAATAATTGAAAAAGAAAAATATTTGGCAATACAATAAAGACAACCAAAGCAATGAACCCGTTTCATTGCTTTTTATCGCCTCGGAAAAGAAAAGTGCGGTCGGAATTTTAGGTGTTTCACATCGAAAAAACACAGGAAAAATCCACCGCACTTTTTAAAACCCGACCAGTGTGGTGAATTCCATGGTTTCGCCGGTGATCGGGTGCCGGA > 3/30424‑30914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |