Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	20,623	T→C	I51V (ATA→GTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	20,623	0	T	C	100.0%	107.5 / NA	44	I51V (ATA→GTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/11);  total (33/11)

GAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTTAGTATAAGGCGATTTTATGCCATCAAGCCCTCTTTCACGAAAGGCTTTTAACCAATAAATGACTTGAGAATGAGGAATCTTA  >  3/20377‑20687                                                                                                                                                                                                                                                       |                                                                 gAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCAt                                                             >  1:418947/1‑251 (MQ=255)        tGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTCTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGccc                                                      <  1:748636/251‑1 (MQ=255)         gAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGcc                                                       >  1:800173/1‑249 (MQ=255)          aGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCct                                                     <  1:186805/250‑1 (MQ=255)           gCTCGGTATTGTGCCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTATGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCct                                                     >  1:295728/1‑249 (MQ=18)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:893507/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:545060/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:139381/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:140127/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:575498/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:80749/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:380187/1‑250 (MQ=255)                gTATTGTACCAGGCTCAATACTTTTAAACCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGCTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:578528/1‑250 (MQ=18)                  aTTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGTGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGa                                            <  1:271754/251‑1 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:315628/1‑251 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:727300/1‑251 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:210409/1‑251 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:44344/1‑251 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:145750/1‑251 (MQ=255)                     gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:513488/1‑251 (MQ=255)                       aCCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGc                                       <  1:112192/251‑1 (MQ=255)                           ggCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGaa                                           <  1:717998/243‑1 (MQ=255)                            gCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCtttt                                   >  1:730491/1‑250 (MQ=255)                             cTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCttttcc                                 <  1:472157/251‑3 (MQ=255)                                 aTACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCttttccaata                             >  1:329574/1‑245 (MQ=255)                                    cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTTTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:155611/1‑251 (MQ=17)                                    cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:240872/1‑251 (MQ=255)                                    cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:472045/1‑251 (MQ=255)                                    cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:395212/1‑251 (MQ=255)                                             cGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTgaga               <  1:687968/251‑1 (MQ=255)                                                gTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGaacg            >  1:349475/1‑249 (MQ=14)                                                         gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTgag                <  1:736955/238‑1 (MQ=14)                                                         gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:10064/1‑248 (MQ=11)                                                         gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:777479/1‑248 (MQ=11)                                                         gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:749024/1‑248 (MQ=11)                                                         gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:500863/1‑248 (MQ=11)                                                          tttttGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAtttag  <  1:660182/251‑5 (MQ=11)                                                                    ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGc                                                        >  1:267605/1‑189 (MQ=255)                                                                    ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCt                                      >  1:803867/1‑207 (MQ=255)                                                                    ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTg                  >  1:647501/1‑225 (MQ=11)                                                                    ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttt    <  1:270846/239‑3 (MQ=11)                                                                         aTTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATg                       >  1:621030/1‑215 (MQ=11)                                                                            ccACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCAc                                              <  1:892074/191‑1 (MQ=255)                                                                                                                       gggTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGc                                       >  1:110507/1‑155 (MQ=11)                                                                                                                                                                                                                                                       |                                                                 GAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTTAGTATAAGGCGATTTTATGCCATCAAGCCCTCTTTCACGAAAGGCTTTTAACCAATAAATGACTTGAGAATGAGGAATCTTA  >  3/20377‑20687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑