Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	20,615	C→T	S53S (TCG→TCA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	20,615	0	C	T	100.0%	117.2 / NA	49	S53S (TCG→TCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/13);  total (36/13)

CAGTTAACTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTTAGTATAAGGCGATTTTATGCCATCAAGCCCTCTTTCACGAAAGGCTTTTAACCAATAAATGACTTGAGAATGAGGAATCTTA  >  3/20368‑20687                                                                                                                                                                                                                                                        |                                                                         cAGTTAACTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGAtt                                                                      >  1:684093/1‑251 (MQ=255)      aaCTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTacg                                                                 >  1:113399/1‑249 (MQ=255)      aaCTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTacg                                                                 <  1:188471/251‑3 (MQ=255)       aCTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTacgc                                                                <  1:604267/251‑4 (MQ=255)        cTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTacgc                                                                >  1:362657/1‑247 (MQ=255)          gAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCAt                                                             >  1:418947/1‑251 (MQ=255)                 tGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTCTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGccc                                                      <  1:748636/251‑1 (MQ=255)                  gAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGcc                                                       >  1:800173/1‑249 (MQ=255)                   aGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCct                                                     <  1:186805/250‑1 (MQ=255)                    gCTCGGTATTGTGCCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTATGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCct                                                     >  1:295728/1‑249 (MQ=18)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:893507/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:575498/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:545060/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:380187/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:80749/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:139381/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:140127/1‑250 (MQ=255)                         gTATTGTACCAGGCTCAATACTTTTAAACCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGCTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCa                                               >  1:578528/1‑250 (MQ=18)                           aTTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGTGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGa                                            <  1:271754/251‑1 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:44344/1‑251 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:513488/1‑251 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:145750/1‑251 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:727300/1‑251 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:210409/1‑251 (MQ=255)                              gTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAg                                         >  1:315628/1‑251 (MQ=255)                                aCCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGc                                       <  1:112192/251‑1 (MQ=255)                                    ggCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGaa                                           <  1:717998/243‑1 (MQ=255)                                     gCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCtttt                                   >  1:730491/1‑250 (MQ=255)                                      cTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCttttcc                                 <  1:472157/251‑3 (MQ=255)                                          aTACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCttttccaata                             >  1:329574/1‑245 (MQ=255)                                             cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTTTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:155611/1‑251 (MQ=17)                                             cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:472045/1‑251 (MQ=255)                                             cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:240872/1‑251 (MQ=255)                                             cTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAAt                        >  1:395212/1‑251 (MQ=255)                                                      cGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTgaga               <  1:687968/251‑1 (MQ=255)                                                         gTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGaacg            >  1:349475/1‑249 (MQ=14)                                                                  gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTgag                <  1:736955/238‑1 (MQ=14)                                                                  gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:500863/1‑248 (MQ=11)                                                                  gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:10064/1‑248 (MQ=11)                                                                  gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:749024/1‑248 (MQ=11)                                                                  gTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttta   >  1:777479/1‑248 (MQ=11)                                                                   tttttGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAtttag  <  1:660182/251‑5 (MQ=11)                                                                             ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGc                                                        >  1:267605/1‑189 (MQ=255)                                                                             ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCt                                      >  1:803867/1‑207 (MQ=255)                                                                             ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTg                  >  1:647501/1‑225 (MQ=11)                                                                             ccccAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATGACTTGAGAACGAGAAAttt    <  1:270846/239‑3 (MQ=11)                                                                                  aTTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGCTTTT‑‑CCAATAAATg                       >  1:621030/1‑215 (MQ=11)                                                                                     ccACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCAc                                              <  1:892074/191‑1 (MQ=255)                                                                                                                                gggTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTT‑GTGTAAGGTGATTTTACGCCATCAAGCCCTCTTTCACGAAAGGc                                       >  1:110507/1‑155 (MQ=11)                                                                                                                                                                                                                                                        |                                                                         CAGTTAACTGAAGTATTGAGCTCGGTATTGTACCAGGCTCAATACTTTTAAATCGAGTTTAATTTGTTTTTGATCGCCCCAATTCCACTAACTTTTTTAGATAATCAATCTCCGCTTTTTCAAGGGCGGGTTCTCGTTTCAGTTTTTTAAAGGCTTGTGGTGAAAAGTCGGTTGTTTCAGCCACTTCAATCTGCGTTTTTTTCATTTTGGGTTTCAGAATTTTGGAGGTTTTAGGTTTAGTATAAGGCGATTTTATGCCATCAAGCCCTCTTTCACGAAAGGCTTTTAACCAATAAATGACTTGAGAATGAGGAATCTTA  >  3/20368‑20687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑