Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	385,410	A→C	Q263P (CAA→CCA)	unknown →	Inositol transport system sugar‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	385,410	0	A	C	100.0%	219.4 / NA	79	Q263P (CAA→CCA)	unknown	Inositol transport system sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/32);  total (47/32)

TTAAGGTGGTGGCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCAATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCAAATTCTCCGTATATT  >  1/385164‑385653                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ttAAGGTGGTGGCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCg                                                                                                                                                                                                                                                 >  1:161553/1‑251 (MQ=255)     ggtggtGGCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCAt                                                                                                                                                                                                                                             >  1:299176/1‑251 (MQ=255)          tggCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATcaggg                                                                                                                                                                                                                                        <  1:483680/251‑1 (MQ=255)            gCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATcagggc                                                                                                                                                                                                                                       <  1:52324/250‑1 (MQ=255)                aaCAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  <  1:888684/251‑1 (MQ=255)                aaCAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  <  1:292228/251‑1 (MQ=255)                aaCAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  <  1:738012/251‑1 (MQ=255)                     gagGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGttt                                                                                                                                                                                                                             >  1:190837/1‑251 (MQ=255)                      agGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTg                                                                                                                                                                                                                            >  1:506750/1‑251 (MQ=255)                      agGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATCGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTg                                                                                                                                                                                                                            >  1:881221/1‑251 (MQ=255)                       ggCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  >  1:717910/1‑244 (MQ=255)                        gCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTg                                                                                                                                                                                                                            <  1:145387/249‑1 (MQ=255)                           aaaTGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  <  1:829190/240‑1 (MQ=255)                           aaaTGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTg                                                                                                                                                                                                                        >  1:874065/1‑250 (MQ=255)                           aaaTGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTg                                                                                                                                                                                                                        >  1:78675/1‑250 (MQ=255)                           aaaTGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGc                                                                                                                                                                                                                       <  1:319860/251‑1 (MQ=255)                                 gACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGaaa                                                                                                                                                                                                                  <  1:711368/250‑1 (MQ=255)                                      aTCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCAt                                                                                                                                                                                                            >  1:109738/1‑251 (MQ=255)                                          aaaGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACaa                                                                                                                                                                                                        <  1:549846/251‑1 (MQ=255)                                              gCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGc                                                                                                                                                                                                     <  1:201860/250‑1 (MQ=255)                                                             aaaaTCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGAc                                                                                                                                                                                     <  1:252165/251‑1 (MQ=255)                                                                           gCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATaa                                                                                                                                                                       >  1:7118/1‑251 (MQ=255)                                                                               aTCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTgg                                                                                                                                                                   >  1:286527/1‑251 (MQ=255)                                                                                 cgcgATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGAt                                                                                                                                                                 >  1:238479/1‑251 (MQ=255)                                                                                     aTGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTc                                                                                                                                                               >  1:197473/1‑249 (MQ=255)                                                                                           aTGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGaa                                                                                                                                                       >  1:151954/1‑251 (MQ=255)                                                                                               gATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  <  1:516241/172‑1 (MQ=255)                                                                                               gATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGaa                                                                                                                                                       <  1:643979/247‑1 (MQ=255)                                                                                                     gAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTg                                                                                                                                                                    >  1:342461/1‑228 (MQ=255)                                                                                                     gAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGAt                                                                                                                                                                 >  1:205839/1‑231 (MQ=255)                                                                                                       gTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTAAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACAcc                                                                                                                                            >  1:455788/1‑250 (MQ=255)                                                                                                          ataatGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGat                                                                                                                                        >  1:141399/1‑251 (MQ=255)                                                                                                           taatGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGata                                                                                                                                       <  1:723721/251‑1 (MQ=255)                                                                                                             atGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATg                                                                                                                                     <  1:617892/251‑1 (MQ=255)                                                                                                               gACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGc                                                                                                                                                                                                                       >  1:649323/1‑167 (MQ=255)                                                                                                                 cGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAgg                                                                                                                                 <  1:699485/251‑1 (MQ=255)                                                                                                                      tGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCaa                                                                                                                            <  1:193928/251‑1 (MQ=255)                                                                                                                                cgcATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATcaaca                                                                                                                  <  1:384437/251‑1 (MQ=255)                                                                                                                                         cgcgGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAgg                                                                                                         <  1:694092/251‑1 (MQ=255)                                                                                                                                                  gAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAAt                                                                                                  <  1:465050/249‑1 (MQ=255)                                                                                                                                                  gAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAAt                                                                                                  <  1:767031/249‑1 (MQ=255)                                                                                                                                                  gAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTg                                                                                                >  1:408115/1‑251 (MQ=255)                                                                                                                                                   aaaCTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCg                                                                                                                                                                                          >  1:749049/1‑160 (MQ=255)                                                                                                                                                   aaaCTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTg                                                                                                <  1:727321/250‑1 (MQ=255)                                                                                                                                                          gCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAAt                                                                                                  >  1:679275/1‑241 (MQ=255)                                                                                                                                                          gCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCa                                                                                          >  1:98571/1‑249 (MQ=255)                                                                                                                                                            tttAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACttt                                                                                      <  1:108721/251‑1 (MQ=255)                                                                                                                                                                  gATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTataacg                                                                                >  1:633728/1‑251 (MQ=255)                                                                                                                                                                   aTGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTataacga                                                                               >  1:187075/1‑251 (MQ=255)                                                                                                                                                                   aTGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTataac                                                                                 <  1:506080/249‑1 (MQ=255)                                                                                                                                                                   aTGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTataac                                                                                 <  1:157970/249‑1 (MQ=255)                                                                                                                                                                        gATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAAt                                                                                                                                                      <  1:17707/175‑1 (MQ=255)                                                                                                                                                                            tGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTataac                                                                                 >  1:251553/1‑240 (MQ=255)                                                                                                                                                                                        gCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAgggg                                                                                                                                                                                                                                  >  1:790495/1‑83 (MQ=255)                                                                                                                                                                                        gCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAatatat                                                                                                                       >  1:33666/1‑190 (MQ=255)                                                                                                                                                                                                 cgACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAAcc                                                 <  1:37475/251‑1 (MQ=255)                                                                                                                                                                                                   acacCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGa                                               >  1:285071/1‑251 (MQ=255)                                                                                                                                                                                                      ccGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATc                                                                                                                      >  1:464530/1‑177 (MQ=255)                                                                                                                                                                                                            gCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAg                                      >  1:367843/1‑251 (MQ=255)                                                                                                                                                                                                              gTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGtt                                    >  1:617466/1‑251 (MQ=255)                                                                                                                                                                                                                 ggggTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAatatat                                                                                                                       >  1:847845/1‑165 (MQ=255)                                                                                                                                                                                                                 ggggTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTaaa                                 >  1:548608/1‑251 (MQ=255)                                                                                                                                                                                                                    gTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAg                                                                <  1:217077/217‑1 (MQ=255)                                                                                                                                                                                                                    gTATTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAgg                                        >  1:578562/1‑241 (MQ=255)                                                                                                                                                                                                                      aTTTAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAgg                                                                                                                                                                                            >  1:245662/1‑91 (MQ=255)                                                                                                                                                                                                                       tttAGGTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATc                                                                                                                      >  1:846188/1‑160 (MQ=255)                                                                                                                                                                                                                            gTAACGGTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGGTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAAc                       <  1:426756/250‑1 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGAc                                                                                                                                                                                     >  1:518306/1‑86 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGAc                                                                                                                                                                                     >  1:572343/1‑86 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTAtaacgataacga                                                                         >  1:469009/1‑194 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAgg                                        >  1:864536/1‑227 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGa                                       <  1:122031/228‑1 (MQ=255)                                                                                                                                                                                                                                  gTCTTGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCaa                >  1:377851/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGACGCGACTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAgg                                                                                                                                 >  1:65050/1‑134 (MQ=255)                                                                                                                                                                                                                                             aCTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCaa                                                                                                                            <  1:129520/132‑1 (MQ=255)                                                                                                                                                                                                                                             aCTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCAAATTCTCCGtat     >  1:140212/1‑251 (MQ=255)                                                                                                                                                                                                                                              cTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAAc                                                  >  1:485508/1‑205 (MQ=255)                                                                                                                                                                                                                                              cTGTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCAAATTCTCCGtata    >  1:872269/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTTTATCCATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCAAATTCTCCGTATAtt  >  1:321529/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TTAAGGTGGTGGCGGAACAAGAGGCAAAATGGGACCGATCAAAAGGCATGGAAGTGGCGGAAAATCTGCTTTCGGCACATCGCGATGCCAATGTGATTTTGAGTAATAATGACGAAATGGCAATCGGCGCATTGCTCGCGGCGAGGAAACTGGGCTTTAAAGATGAAGATTTGCTTATTGTCGGCATTGACGCGACACCGGATGCGTTGGGGTATTTAGGTAACGGTCTTGACGCGACTGTTTATCAATCGGCATCAGGGCAGGGGCGTTTGAGTGCAGAAATGGCATACAAAGCTGCGTTAGGCGAAGACATTCCGAAATATAACTGGATTCCTTTTGAATTGGTGACACCCGATATGAAGGAGCAATATATCAACAAATATAAGGAGTAAATTGACCGCACTTTATAACGATAACGACGATTTTAGTATTCGTTCTTAACCGATTTAAGGAGTTAAAAATGACTAACAGTTCAAATTCTCCGTATATT  >  1/385164‑385653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑