BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	3	13,491	A→C	L177R (CTC→CGC)	unknown ←	Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	3	13,491	0	A	C	100.0%	275.3 / NA	104	L177R (CTC→CGC)	unknown	Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (61/43); total (61/43)

Only 100 of 104 total aligned reads displayed.

GGGCAAAATAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGAGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGTCAT  >  3/13246‑13731

gggCAAAATAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTAt                                                                                                                                                                                                                                              >  1:42902/1‑251 (MQ=255)

gggCAAAATAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTAt                                                                                                                                                                                                                                              >  1:633523/1‑251 (MQ=255)

 ggCAAAATAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTAt                                                                                                                                                                                                                                              >  1:816531/1‑250 (MQ=255)

    aaaaTAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTcc                                                                                                                                                                                                                                          <  1:209837/251‑1 (MQ=255)

     aaaTAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTccc                                                                                                                                                                                                                                         <  1:407338/251‑1 (MQ=255)

     aaaTAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTccc                                                                                                                                                                                                                                         <  1:813833/251‑1 (MQ=255)

             atatTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTaa                                                                                                                                                                                                                                 >  1:117385/1‑251 (MQ=255)

             atatTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTaa                                                                                                                                                                                                                                 >  1:201869/1‑251 (MQ=255)

             atatTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTaa                                                                                                                                                                                                                                 >  1:684402/1‑251 (MQ=255)

                       ccATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAAcc                                                                                                                                                                                                                               <  1:144911/243‑1 (MQ=255)

                           gATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCg                                                                                                                                                                                                                              <  1:514579/240‑1 (MQ=255)

                           gATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAg                                                                                                                                                                                                                    >  1:5955/1‑250 (MQ=255)

                           gATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAg                                                                                                                                                                                                                    >  1:657512/1‑250 (MQ=255)

                            aTTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAg                                                                                                                                                                                                                    >  1:703511/1‑249 (MQ=255)

                                 cGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACgt                                                                                                                                                                                                             >  1:807535/1‑251 (MQ=255)

                                   aTGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCg                                                                                                                                                                                                                              <  1:426061/232‑1 (MQ=255)

                                   aTGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACgtgt                                                                                                                                                                                                           >  1:671362/1‑251 (MQ=255)

                                     gtgtCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTAtctc                                                                                                                                                                                                                                           <  1:888029/217‑1 (MQ=255)

                                     gtgtCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCAc                                                                                                                                                                                                               >  1:8916/1‑245 (MQ=255)

                                     gtgtCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTc                                                                                                                                                                                                          >  1:310090/1‑250 (MQ=255)

                                       gtCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCAt                                                                                                                                                                                                       >  1:82107/1‑251 (MQ=255)

                                         cccTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCAttg                                                                                                                                                                                                     <  1:1425/251‑1 (MQ=255)

                                          ccTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCAttgt                                                                                                                                                                                                    <  1:483641/251‑1 (MQ=255)

                                             tCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTccc                                                                                                                                                                                                                                         <  1:619922/211‑1 (MQ=255)

                                                  gCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCg                                                                                                                                                                                                                                    <  1:244961/211‑1 (MQ=255)

                                                  gCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCg                                                                                                                                                                                              >  1:549964/1‑249 (MQ=255)

                                                  gCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCg                                                                                                                                                                                              >  1:451959/1‑249 (MQ=255)

                                                  gCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGc                                                                                                                                                                                             <  1:323671/250‑1 (MQ=255)

                                                      tCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGc                                                                                                                                                                                        <  1:539193/251‑1 (MQ=255)

                                                       cACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGcc                                                                                                                                                                                                                                     <  1:1433/205‑1 (MQ=255)

                                                       cACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCa                                                                                                                                                                                       >  1:888401/1‑251 (MQ=255)

                                                       cACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCa                                                                                                                                                                                       >  1:554426/1‑251 (MQ=255)

                                                       cACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCa                                                                                                                                                                                       >  1:549306/1‑251 (MQ=255)

                                                       cACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCa                                                                                                                                                                                       >  1:249052/1‑251 (MQ=255)

                                                        aCGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑g                                                                                                                                                                                     >  1:199241/1‑251 (MQ=255)

                                                         cGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑gg                                                                                                                                                                                    >  1:333313/1‑251 (MQ=255)

                                                                  cTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGa                                                                                                                                                                           >  1:339727/1‑251 (MQ=255)

                                                                            gTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGc                                                                                                                                                                                        >  1:682381/1‑229 (MQ=255)

                                                                               gTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTcc                                                                                                                                                                                                         <  1:869267/209‑1 (MQ=255)

                                                                               gTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTg                                                                                                                                                                >  1:455710/1‑249 (MQ=255)

                                                                               gTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGGGAAt                                                                                                                                                                         >  1:409142/1‑240 (MQ=255)

                                                                                tCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCt                                                                                                                                                              <  1:104764/250‑1 (MQ=255)

                                                                                tCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCCCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCt                                                                                                                                                              >  1:416971/1‑250 (MQ=255)

                                                                                   tcattcaGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCgg                                                                                                                                                           >  1:168107/1‑250 (MQ=255)

                                                                                   tcattcaGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGc                                                                                                                                                          <  1:131643/251‑1 (MQ=255)

                                                                                    cattcaGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCg                                                                                                                                                         >  1:743061/1‑251 (MQ=255)

                                                                                        caGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTc                                                                                                                                                                                                                            <  1:14583/181‑1 (MQ=255)

                                                                                          gCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGt                                                                                                                                                                             >  1:766616/1‑225 (MQ=255)

                                                                                           ccAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGc                                                                                                                                                   <  1:539485/250‑1 (MQ=255)

                                                                                            cAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCAc                                                                                                                                                 <  1:778093/251‑1 (MQ=255)

                                                                                                    ggcgAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCg                                                                                                                                                                                                                                    <  1:541841/161‑1 (MQ=255)

                                                                                                              cccTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTc                                                                                                                               <  1:565568/251‑1 (MQ=255)

                                                                                                                cTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAg                                                                                                                             <  1:681505/251‑1 (MQ=255)

                                                                                                                   aTTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTaa                                                                                                                          <  1:611304/251‑1 (MQ=255)

                                                                                                                    ttttCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAAt                                                                                                                         >  1:449506/1‑251 (MQ=255)

                                                                                                                    ttttCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAAt                                                                                                                         >  1:872163/1‑251 (MQ=255)

                                                                                                                     tttCAATGTGTTCTGCATGATTGGTGAGTTTCCCCGGCGCCGGCATCGGTTGCGGCAGCATAAATTCCTCGTTGTCCGTTGGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATc                                                                                                                        <  1:804905/251‑1 (MQ=255)

                                                                                                                     tttCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATc                                                                                                                        <  1:255403/251‑1 (MQ=255)

                                                                                                                        cAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCAc                                                                                                                      >  1:417142/1‑250 (MQ=255)

                                                                                                                         aaTGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGAc                                                                                                                                                                                                >  1:707570/1‑176 (MQ=255)

                                                                                                                         aaTGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGACACGCACTTTGTCCGCCAATACTTCAGTAATCACCg                                                                                                                    >  1:16101/1‑251 (MQ=255)

                                                                                                                          aTGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCGGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGc                                                                                                                   <  1:879528/251‑1 (MQ=255)

                                                                                                                            gtgtTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCca                                                                                                                 >  1:674916/1‑251 (MQ=255)

                                                                                                                              gtTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACt                                                                                                                                 >  1:516483/1‑233 (MQ=255)

                                                                                                                              gtTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCcaca                                                                                                               >  1:22700/1‑251 (MQ=255)

                                                                                                                              gtTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCcaca                                                                                                               >  1:301892/1‑251 (MQ=255)

                                                                                                                              gtTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGCATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGGGAATCACGTTTTGCTCGGCGATACGCACCTTGTCCGCCAATACTTCAGGAATCACCGCcaca                                                                                                               >  1:640607/1‑251 (MQ=255)

                                                                                                                                 cTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATg                                                                                                             >  1:608262/1‑250 (MQ=255)

                                                                                                                                   gCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGGTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTaa                                                                                                                          >  1:353880/1‑235 (MQ=255)

                                                                                                                                    cATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGccc                                                                                                          <  1:505803/250‑1 (MQ=255)

                                                                                                                                        aaTGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGgtg                                                                                                      <  1:577411/250‑1 (MQ=255)

                                                                                                                                        aaTGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGgtg                                                                                                      <  1:403755/250‑1 (MQ=255)

                                                                                                                                         aTGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGtgtg                                                                                                    >  1:812354/1‑251 (MQ=255)

                                                                                                                                           ggTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCAttgt                                                                                                                                                                                                    >  1:490328/1‑154 (MQ=255)

                                                                                                                                                gTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGCCGGTATCTCCCAGCCGTAACCGTCCTCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTaaa                                                                                              <  1:549438/250‑1 (MQ=255)

                                                                                                                                                 tttCCCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTaaa                                                                                              <  1:128927/249‑1 (MQ=255)

                                                                                                                                                   tctcCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGc                                                                                                                                                                                        >  1:169623/1‑158 (MQ=255)

                                                                                                                                                                               aCTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCggg                                                                                                                                                                                <  1:580408/137‑1 (MQ=255)

                                                                                                                                                                               aCTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCGCGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGcc                                                               >  1:245630/1‑250 (MQ=255)

                                                                                                                                                                                cTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCca                                                                                                                 >  1:15374/1‑199 (MQ=255)

                                                                                                                                                                                cTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGccc                                                              >  1:647784/1‑250 (MQ=255)

                                                                                                                                                                                  aatttCCTCGTTGTCCGTTGGAACCCTCCAGCCGAGATTTCCCGTGTCATCAAGGGTGTCTTGCGGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTGGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGc                                                           <  1:462034/248‑1 (MQ=255)

                                                                                                                                                                                    aTTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATa                                                                                                                                                      <  1:476145/158‑1 (MQ=255)

                                                                                                                                                                                      cccTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTc                                                       <  1:722857/250‑1 (MQ=255)

                                                                                                                                                                                                       aaTCACCCAGCCGAGAATTTCCGTGTCATCAAGGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTgccg                                       <  1:248964/250‑1 (MQ=255)

                                                                                                                                                                                                           atcCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGtt                                  >  1:111120/1‑251 (MQ=255)

                                                                                                                                                                                                           atcCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTGCCAGGCGGAATCTCCCGGCCGTAACGGTCATCGGCAGCTCACGTGCCCTTTGTTGACCGCTT‑GCAGGGGGGGGGGGAATCAGGTTTTGCTCGGCGATTGCCACTTTGTCCCCCAAAACTTCAGTAATCACCCCCCCTTCCCCGGGTTGCTTAAATTTCCCGCCTTCCTGCCAAATGGGCATTGCCCCCCTTTCTGGGCCCCGTTTgcccccttt                                  >  1:790902/1‑245 (MQ=255)

                                                                                                                                                                                                                gCCGAGAATTTCCGTGTCATCAAAGGTGTCTCCCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCgg                                                                                                        <  1:876723/176‑1 (MQ=255)

                                                                                                                                                                                                                    agaATTCCCGTGCAATCAAGGGTGTCTGGCGGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAgg                          <  1:77221/250‑1 (MQ=255)

                                                                                                                                                                                                                        tttCCGTGTCATCAAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGc                                                                                                                                                               <  1:700233/113‑1 (MQ=255)

                                                                                                                                                                                                                                 catcaAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGccc                                                              >  1:885654/1‑201 (MQ=255)

                                                                                                                                                                                                                                 catcaAAGGTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCAt                                                         >  1:573307/1‑206 (MQ=255)

                                                                                                                                                                                                                                    caagggTGTCTTGCAGGCGTTATCCCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGccgccg                                    <  1:273255/220‑1 (MQ=255)

                                                                                                                                                                                                                                        ggTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGt     >  1:63137/1‑251 (MQ=255)

                                                                                                                                                                                                                                        ggTGTCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCCGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCCCCGTTGGCAAAGGCTTGCAACGGCAACCGGGCgg     >  1:526976/1‑250 (MQ=255)

                                                                                                                                                                                                                                         gtgtCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGcc                                                                                                           >  1:299361/1‑148 (MQ=255)

                                                                                                                                                                                                                                           gtCTTGCAGGCGTTATCCCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGcc                                                                                                           <  1:826951/146‑1 (MQ=255)

                                                                                                                                                                                                                                           gtCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGTCAt  >  1:131320/1‑251 (MQ=255)

                                                                                                                                                                                                                                           gtCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGTCAt  >  1:833071/1‑251 (MQ=255)

                                                                                                                                                                                                                                           gtCTTGCAGGCGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGTCAt  >  1:176614/1‑251 (MQ=255)

GGGCAAAATAGCGATATTGATCGCCATGATTGACGATGTGTCCCTTCATTGCCTTCACGTAAGCCGCTTCCAGCGGGTTGTCTTCATTCAGCCAATTGGCGGCGAATTGTCCCTGATTTTCAATGTGTTCTGCATGAATGGTGAGTTTCTCCGGCGCCGGCATCGGTTGCGGCAGACTAAATTCCTCGTTGTCCGTTTGAATCATCCAGCCGAGAATTTCCGTGTCATCAAAGGTGTCTTGCAGGAGGTATCTCCCAGCCGTAACCGTCATCGGCAGCTCACGTGTCCATTGTTGACCGCTTGGCA‑GGCGGGAGTGAATCACGTTTTGCTCGGCGATACGCACTTTGTCCGCCAATACTTCAGTAATCACCGCCACATGCCCGGTGTGCTTAAATTCGCCGCCTTCCTGCCAAATGAGCAATGCCCCGCATTCCGGCGCCCGTTTGCCGCCGTTGGCAAAGGCTTGCAACGGCAACAGGGCGTCAT  >  3/13246‑13731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`