Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 37,641 A→G Y6Y (TAT→TAC)  unknown ← L‑threonate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*137,6410AG100.0% 117.6 / NA 42Y6Y (TAT→TAC) unknownL‑threonate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/17);  total (25/17)

TAGCCGCTAAACCATTTTCACCAAATAGCACCGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAATATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTATGATTCCTGCAGAGCGACAAA  >  1/37394‑37872
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
tAGCCGCTAAACCATTTTCACCAAATAGCACCGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAgtat                                                                                                                                                                                                                                      <  1:480588/251‑1 (MQ=255)
      cTAAACCATTTTCACCAAATAGCACCGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGTAAAATCGACCGCACTTTGGCTGGCTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAtttg                                                                                                                                                                                                                                <  1:443710/251‑1 (MQ=255)
      cTAAACCATTTTCACCAAATAGCACCGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAtttg                                                                                                                                                                                                                                <  1:504922/251‑1 (MQ=255)
                              ccGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAt                                                                                                                                                                                                                                   >  1:865164/1‑224 (MQ=255)
                                     gACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAt                                                                                                                                                                                                                                   >  1:349380/1‑217 (MQ=255)
                                          gCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAtttgtttg                                                                                                                                                                                                                            >  1:223150/1‑219 (MQ=255)
                                          gCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTttgtt                                                                                                                                                                                            >  1:192310/1‑251 (MQ=255)
                                                 aTTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAt                                                                                                                                                                                                                                   >  1:52247/1‑205 (MQ=255)
                                                    tactacAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACgg                                                                                                                                                                                  <  1:302992/251‑1 (MQ=255)
                                                    tactacAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACgg                                                                                                                                                                                  <  1:897904/251‑1 (MQ=255)
                                                    tactacAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACgg                                                                                                                                                                                  >  1:543238/1‑251 (MQ=255)
                                                             caacaaAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAaataat                                                                                                                                                                                                      >  1:335062/1‑222 (MQ=255)
                                                                caaAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGc                                                                                                                                                                      <  1:731297/251‑1 (MQ=255)
                                                                                           aTCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGc                                                                                                                                           <  1:894963/251‑1 (MQ=255)
                                                                                                             ggTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAaat                                                                                                                                                                                                         <  1:571820/171‑1 (MQ=255)
                                                                                                             ggTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTaa                                                                                                                          >  1:561093/1‑250 (MQ=255)
                                                                                                               ttGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACat                                                                                                                       <  1:568026/251‑1 (MQ=255)
                                                                                                                                                  gCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGt                                                                                                                                      >  1:791441/1‑201 (MQ=255)
                                                                                                                                                        gggTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAAc                                                                                                                         >  1:587504/1‑208 (MQ=255)
                                                                                                                                                                    acacCATAGGACGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTAt                                                                                                                                                                                                                   <  1:593947/106‑1 (MQ=255)
                                                                                                                                                                     cacCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGc                                                                                                                                           >  1:855122/1‑177 (MQ=255)
                                                                                                                                                                     cacCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATa                                                                 >  1:678377/1‑251 (MQ=255)
                                                                                                                                                                       ccATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGAt                                                                                                                                                                                                                                   <  1:695820/87‑1 (MQ=255)
                                                                                                                                                                                          ctctTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAAc                                                                                                                                                                                    >  1:124031/1‑115 (MQ=255)
                                                                                                                                                                                          ctctTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATaacaac                                                                       <  1:100388/224‑1 (MQ=255)
                                                                                                                                                                                             ttATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGa                                                                               >  1:619806/1‑213 (MQ=255)
                                                                                                                                                                                               ataCAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTc                                                                                                                                                                         <  1:901712/121‑1 (MQ=255)
                                                                                                                                                                                               ataCAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATc                                                                                                                                                                <  1:806521/130‑1 (MQ=255)
                                                                                                                                                                                               ataCAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGt                                                                                                                                      <  1:847543/156‑1 (MQ=255)
                                                                                                                                                                                                    aGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGt                                                                                                                                                                                                                     >  1:624210/1‑72 (MQ=255)
                                                                                                                                                                                                    aGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACat                                                                                                                       >  1:501345/1‑166 (MQ=255)
                                                                                                                                                                                                    aGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAAttttt                                  >  1:444166/1‑251 (MQ=255)
                                                                                                                                                                                                               cccccATGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTa                       >  1:117277/1‑251 (MQ=255)
                                                                                                                                                                                                                     tGCCCATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGtt                                                                                                                                                                                                >  1:781939/1‑76 (MQ=255)
                                                                                                                                                                                                                         cATTGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAGCAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTATGATTCCTGc             <  1:885140/251‑1 (MQ=255)
                                                                                                                                                                                                                           ttGCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTATGATTCCTGCag           >  1:891994/1‑251 (MQ=255)
                                                                                                                                                                                                                             gCACCCAGCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACaaaa                                                                                                                               >  1:13931/1‑133 (MQ=255)
                                                                                                                                                                                                                                    gCCCAACAATAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTATGATTCCTGCAGAGCGACaaa  >  1:854603/1‑251 (MQ=255)
                                                                                                                                                                                                                                       caacaaTAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAAc                                                                                                                         <  1:336208/129‑1 (MQ=255)
                                                                                                                                                                                                                                         acaaTAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTc                                                                                                                                                                         <  1:180119/79‑1 (MQ=255)
                                                                                                                                                                                                                                          caaTAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGc                                                                                                                                                                      >  1:874443/1‑81 (MQ=255)
                                                                                                                                                                                                                                          caaTAGCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTAttattt                                                                                                               >  1:206348/1‑136 (MQ=255)
                                                                                                                                                                                                                                               gCAACAGAGTATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAag                                                        >  1:533396/1‑186 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
TAGCCGCTAAACCATTTTCACCAAATAGCACCGAATTGACTTGCACAGCATTTACTACAAGCAACAAAACTGCATCTAATTTATCCGCAAAATCGACCGCACTTTGGCTGGTTGCGTGTGCGCCATCAGTTTTTAATTTTTCAAGTGCTTTTGGGTTAAGATCTACACCATAGGTCGTCAATCCGGCTCTTATACAAGACTGAGCCGCCCCCATGCCCATTGCACCCAGCCCAACAATAGCAACAGAATATGATTTGTTTGTCATGGTATTTCTCCAAATAATAAAGTTTGTTGATCTAACGGTATCCATTCTGCCGGATCTTAAAATAAAAAAACGTGAGCAATGTCACAAAATTTAACATTATTATTTATTTTTGTTATTTTATGTGAAAAATGAATGGAATAACAACAAAATAAACAAAAAGAGATATAATGACGAAAATTTTTTGGAGGGGTTATGATTCCTGCAGAGCGACAAA  >  1/37394‑37872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑