Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	148,214	T→C	intergenic (+15/+97)	unknown → / ← unknown	hypothetical protein/N‑acetylmuramic acid 6‑phosphate etherase (EC 4.2.1.126)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	148,214	0	T	C	100.0%	232.6 / NA	81	intergenic (+15/+97)	unknown/unknown	hypothetical protein/N‑acetylmuramic acid 6‑phosphate etherase (EC 4.2.1.126)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/37);  total (44/37)

ATTTAAAGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGTATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGTTTGTTCGGCGGTGGTGGCAT  >  2/148022‑148459                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aTTTAAAGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGc                                                                                                                                                                                                >  1:293618/1‑248 (MQ=255) aTTTAAAGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCttt                                                                                                                                                                                             >  1:139738/1‑251 (MQ=255) aTTTAAAGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCttt                                                                                                                                                                                             >  1:77207/1‑251 (MQ=255)      aaGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAg                                                                                                                                                                                        >  1:510614/1‑251 (MQ=255)              cAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATaaa                                                                                                                                                                                >  1:200391/1‑251 (MQ=255)              cAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATaa                                                                                                                                                                                 <  1:500168/250‑1 (MQ=255)                ggTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGt                                                                                                                                                                              >  1:29296/1‑251 (MQ=255)                ggTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGt                                                                                                                                                                              >  1:507450/1‑251 (MQ=255)                ggTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGt                                                                                                                                                                              >  1:783926/1‑251 (MQ=255)                                gAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCg                                                                                                                                                              >  1:491990/1‑251 (MQ=255)                                gAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGATTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCg                                                                                                                                                              >  1:397666/1‑251 (MQ=255)                                     aGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAaata                                                                                                                                                                                                                                 >  1:300378/1‑179 (MQ=255)                                          cTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCa                                                                                                                                                    >  1:18440/1‑251 (MQ=255)                                          cTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCa                                                                                                                                                    >  1:382762/1‑251 (MQ=255)                                                     gaatTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGtt                                                                                                                                                                                                                                           <  1:368611/153‑1 (MQ=255)                                                      aatTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAgg                                                                                                                                         <  1:711606/250‑1 (MQ=255)                                                          tCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTc                                                                                                                                                                                                                                              <  1:134163/145‑1 (MQ=255)                                                          tCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTc                                                                                                                                    <  1:155452/251‑1 (MQ=255)                                                                    gtgCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAaata                                                                                                                                                                                                                                 >  1:124546/1‑148 (MQ=255)                                                                    gtgCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCtt                                                                                                                           >  1:464740/1‑250 (MQ=255)                                                                            ttCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGt                                                                                                                  <  1:278558/251‑1 (MQ=255)                                                                            ttCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGt                                                                                                                  <  1:668122/251‑1 (MQ=255)                                                                              cGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTg                                                                                                                <  1:676064/251‑1 (MQ=255)                                                                                       atgTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCAc                                                                                                             <  1:723509/245‑1 (MQ=255)                                                                                       atgTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAaca                                                                                                        >  1:648399/1‑250 (MQ=255)                                                                                       atgTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAaca                                                                                                        >  1:313437/1‑250 (MQ=255)                                                                                       atgTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAaca                                                                                                        >  1:258709/1‑250 (MQ=255)                                                                                       atgTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATAGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAaca                                                                                                        >  1:785400/1‑250 (MQ=255)                                                                                           aaaCCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCt                                                                                                   <  1:296908/251‑1 (MQ=255)                                                                                                aaTTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCccacc                                                                                                                                             >  1:712246/1‑204 (MQ=255)                                                                                                 aTTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAg                                                                                                                                                                                                                         <  1:401592/127‑1 (MQ=255)                                                                                                         aTGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATac                                                                                                                                                                                                                                                   >  1:727895/1‑93 (MQ=255)                                                                                                           gCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTaa                                                                                                                               <  1:587197/207‑1 (MQ=255)                                                                                                            cGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATc                                                                                  <  1:546930/251‑1 (MQ=255)                                                                                                              tACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATcc                                                                                 <  1:20151/250‑1 (MQ=255)                                                                                                              tACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATcc                                                                                 <  1:357723/250‑1 (MQ=255)                                                                                                                cccTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGc                                                                                                    >  1:901618/1‑229 (MQ=255)                                                                                                                cccTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAAt                                                                              >  1:299789/1‑251 (MQ=255)                                                                                                                cccTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAAt                                                                              >  1:347730/1‑251 (MQ=255)                                                                                                                cccTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAAt                                                                              >  1:49431/1‑251 (MQ=255)                                                                                                                 ccTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGt                                                                                                                  >  1:731722/1‑214 (MQ=255)                                                                                                                    aaTTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTc                                                                                                                          <  1:94146/203‑1 (MQ=255)                                                                                                                    aaTTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACgg                                                                                                     <  1:419642/224‑1 (MQ=255)                                                                                                                    aaTTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCg                                                                           >  1:573916/1‑250 (MQ=255)                                                                                                                      ttattTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGt                                                                                                                  <  1:888542/209‑1 (MQ=255)                                                                                                                            cTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTaacaac                                                                  <  1:652877/251‑1 (MQ=255)                                                                                                                               aGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAAcat                                                                >  1:658132/1‑250 (MQ=255)                                                                                                                                gATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTAc                                                                                                                                                                                                           >  1:488544/1‑110 (MQ=255)                                                                                                                                gATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTaac                                                                     >  1:157255/1‑244 (MQ=255)                                                                                                                                   aCAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGa                                                           <  1:606383/251‑1 (MQ=255)                                                                                                                                    cAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGAt                                                          <  1:808335/251‑1 (MQ=255)                                                                                                                                    cAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGAt                                                          <  1:614914/251‑1 (MQ=255)                                                                                                                                    cAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATATTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGAt                                                          <  1:197092/251‑1 (MQ=255)                                                                                                                                         aGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGa                                                     >  1:226292/1‑251 (MQ=255)                                                                                                                                         aGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGa                                                     >  1:901490/1‑251 (MQ=255)                                                                                                                                              cATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTAc                                                                                                                                                                                                           >  1:371122/1‑96 (MQ=255)                                                                                                                                              cATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGAt                                                          >  1:282239/1‑241 (MQ=255)                                                                                                                                              cATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTg                                                >  1:292686/1‑251 (MQ=255)                                                                                                                                              cATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTACCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCt                                                                                                                            >  1:302270/1‑175 (MQ=255)                                                                                                                                              cATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTCGCATACACTTTGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTaac                                                                     <  1:442473/230‑1 (MQ=255)                                                                                                                                                tAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGc                                              <  1:870974/251‑1 (MQ=255)                                                                                                                                                 aaaTAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAACCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCa                                             <  1:31480/251‑1 (MQ=255)                                                                                                                                                      gAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGc                                         >  1:705892/1‑250 (MQ=255)                                                                                                                                                       aaTGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGc                                         <  1:465730/249‑1 (MQ=255)                                                                                                                                                        aTGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAgg                                                                                                                                         <  1:358068/152‑1 (MQ=255)                                                                                                                                                        aTGGAAAACTTACTAATGAATAGATAGTAATTCTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAAtt                                                                                                                                                                                                                  >  1:141496/1‑79 (MQ=255)                                                                                                                                                             aaaCTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCg                                       <  1:143652/245‑1 (MQ=255)                                                                                                                                                              aaCTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTtggtgg                                <  1:93421/251‑1 (MQ=255)                                                                                                                                                               aCTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGt                                                                                                                                                                         <  1:534323/113‑1 (MQ=255)                                                                                                                                                               aCTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAaca                                                                                                        >  1:317996/1‑178 (MQ=255)                                                                                                                                                                cTTACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGt                                                                                                                  >  1:683257/1‑167 (MQ=255)                                                                                                                                                                  tACTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGgtgtg                            <  1:511246/251‑1 (MQ=255)                                                                                                                                                                    cTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTc                                                                                                 >  1:836501/1‑180 (MQ=255)                                                                                                                                                                    cTAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTtggt                                  >  1:39496/1‑243 (MQ=255)                                                                                                                                                                     tAATGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAg                         <  1:94733/251‑1 (MQ=255)                                                                                                                                                                        tGAATAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGt                      <  1:803795/251‑1 (MQ=255)                                                                                                                                                                          aaTAGATAGTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTaa                                                                                                                               >  1:200143/1‑144 (MQ=255)                                                                                                                                                                           atagatagTAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGAt                                                          <  1:645493/212‑1 (MQ=255)                                                                                                                                                                                  gTAATTTTTTTGTTGCATACACTTCGTTCCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAAcatca                                                              <  1:713217/201‑1 (MQ=255)                                                                                                                                                                                   tAATTTTTTTGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGTTTGTTcggcgg           <  1:725507/251‑1 (MQ=255)                                                                                                                                                                                      tttttttGTTGCATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGTTTGTTCGGCggtgg        >  1:786860/1‑251 (MQ=255)                                                                                                                                                                                             gttgcATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGCTTGTTCGGCGGTGGTGGCAt  >  1:241146/1‑250 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ATTTAAAGCTGAGCAGGTATACTCCCATTATGAACAAGATTCTTTTAAGAATGAATTTCTTGATGTGGTGCTAGTTTCGGTTGATGATGTAAACCAATTAAAAGATGCGTACCCTAATTATTTCTTAGATACAACTAGCTTCATAAATAGAATGGAAAACTTACTAATGAATAGATAGTAATTTTTTTGTTGTATACACTTCGTTTCTCCAAATAATTTCAAGTCCAATTCCCGTACTTGCCCTCGGCTTTTAAAGCCTATAAAGTGCGGTCGTTTTTTCCGGCGTTTTTCACCCCACCAAGGCGTTCTGTAATCTTCCCTTATGTTGCACTAACACGGCTTCCGCCTGCGTTTTATCCAATCCGCTTAACAACATCATGATCGCGAGTTTGGCATTGCCGTTGGTGGTGTGTAGGGTTTGTTCGGCGGTGGTGGCAT  >  2/148022‑148459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑