BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	140,387	T→C	H75H (CAT→CAC)	unknown →	tRNA 5‑methylaminomethyl‑2‑thiouridine synthase subunit TusD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	140,387	0	T	C	100.0%	363.2 / NA	126	H75H (CAT→CAC)	unknown	tRNA 5‑methylaminomethyl‑2‑thiouridine synthase subunit TusD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (62/64); total (62/64)

Only 100 of 126 total aligned reads displayed.

AGATCAGGATGAATAATTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCATTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACTTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTGCCGCCACGGCATTTTG  >  2/140144‑140631

 gATCAGGATGAATAAGTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGt                                                                                                                                                                                                                                               >  1:102954/1‑251 (MQ=255)

 gATCAGGATGAATAAGTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGt                                                                                                                                                                                                                                               >  1:737420/1‑251 (MQ=255)

  aTCAGGATGAATAAGTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGtt                                                                                                                                                                                                                                              >  1:97041/1‑251 (MQ=255)

        aTGAATAAGTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTgccgc                                                                                                                                                                                                                                         <  1:657504/250‑1 (MQ=255)

                gtCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTtgtg                                                                                                                                                                                                                                                  <  1:563919/232‑1 (MQ=255)

                gtCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACg                                                                                                                                                                                                                                >  1:665322/2‑251 (MQ=255)

                gtCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACg                                                                                                                                                                                                                                >  1:592448/2‑251 (MQ=255)

                 tCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAAc                                                                                                                                                                                                                                 <  1:774441/249‑1 (MQ=255)

                 tCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAAc                                                                                                                                                                                                                                 <  1:302737/249‑1 (MQ=255)

                  cATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACgg                                                                                                                                                                                                                               >  1:721762/1‑250 (MQ=255)

                      gATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACggcgtgg                                                                                                                                                                                                                          >  1:678752/1‑251 (MQ=255)

                            gtgtTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCACTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGcgtggcgtgg                                                                                                                                                                                                                     >  1:884534/1‑250 (MQ=255)

                               ttGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGa                                                                                                                                                                                                                 >  1:709768/1‑251 (MQ=255)

                                tggtggTGAAACAACTGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGAt                                                                                                                                                                                                                <  1:834009/251‑1 (MQ=255)

                                  gtggtgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAg                                                                                                                                                                                                              >  1:45157/1‑251 (MQ=255)

                                   tggtgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAg                                                                                                                                                                                                              <  1:199739/250‑1 (MQ=255)

                                   tggtgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGc                                                                                                                                                                                                             <  1:147844/251‑1 (MQ=255)

                                     gtgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTAGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTa                                                                                                                                                                                                           >  1:455025/1‑251 (MQ=255)

                                      tgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTa                                                                                                                                                                                                           <  1:188209/250‑1 (MQ=255)

                                      tgAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGAACAAGTGCTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAGCGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAGTTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTa                                                                                                                                                                                                           <  1:438251/250‑1 (MQ=255)

                                        aaaCAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTc                                                                                                                                                                                                        <  1:483302/251‑1 (MQ=255)

                                        aaaCAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTc                                                                                                                                                                                                        <  1:169283/251‑1 (MQ=255)

                                        aaaCAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTc                                                                                                                                                                                                        <  1:134111/251‑1 (MQ=255)

                                          acaacCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGG‑TTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTgc                                                                                                                                                                                                     <  1:517399/251‑1 (MQ=255)

                                           caacCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTgc                                                                                                                                                                                                     <  1:671460/251‑1 (MQ=255)

                                                     aCGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATaaa                                                                                                                                                                                           <  1:568833/251‑1 (MQ=255)

                                                          aGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTg                                                                                                                                                                                       >  1:166645/1‑250 (MQ=255)

                                                          aGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTg                                                                                                                                                                                       >  1:44805/1‑250 (MQ=255)

                                                                      tttCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTgg                                                                                                                                                                           >  1:638017/1‑250 (MQ=255)

                                                                                        gCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTg                                                                                                                                                         >  1:823912/1‑250 (MQ=255)

                                                                                         cacaAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGc                                                                                                                                                        >  1:348197/1‑250 (MQ=255)

                                                                                              gTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGAc                                                                                                                                                                      >  1:306200/1‑231 (MQ=255)

                                                                                              gTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCtt                                                                                                                                                  >  1:139404/1‑251 (MQ=255)

                                                                                              gTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCtt                                                                                                                                                  >  1:203484/1‑251 (MQ=255)

                                                                                                   ggTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTg                                                                                                                                             >  1:586561/1‑251 (MQ=255)

                                                                                                    gTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAAc                                                                                                                                                                                                                                 <  1:134321/166‑1 (MQ=255)

                                                                                                    gTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTg                                                                                                                                             <  1:504783/250‑1 (MQ=255)

                                                                                                    gTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑a                                                                                                                                           >  1:845686/1‑251 (MQ=255)

                                                                                                    gTGCAAAAGCAGCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTg                                                                                                                                             <  1:277490/250‑1 (MQ=255)

                                                                                                        aaaaGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTc                                                                                                                                       <  1:184072/251‑1 (MQ=255)

                                                                                                            gCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGc                                                                                                                                    >  1:899276/1‑250 (MQ=255)

                                                                                                            gCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGATGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGc                                                                                                                                    >  1:435874/1‑250 (MQ=255)

                                                                                                                 atatCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGcc                                                                                                                              <  1:264276/251‑1 (MQ=255)

                                                                                                                       gCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCttatta                                                                                                                        >  1:895335/1‑251 (MQ=255)

                                                                                                                        ccAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTAc                                                                                                                       <  1:667117/251‑1 (MQ=255)

                                                                                                                        ccAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTAc                                                                                                                       <  1:309538/251‑1 (MQ=255)

                                                                                                                            aTCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGc                                                                                                                   <  1:404154/251‑1 (MQ=255)

                                                                                                                                     tATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAAc                                                                                                                                                            >  1:341652/1‑202 (MQ=255)

                                                                                                                                          ggATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATaa                                                                                                     >  1:764509/1‑251 (MQ=255)

                                                                                                                                          ggATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATaa                                                                                                     >  1:839984/1‑251 (MQ=255)

                                                                                                                                          ggATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATaa                                                                                                     >  1:690642/1‑251 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:651134/250‑1 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:260862/250‑1 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:534039/250‑1 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:167322/250‑1 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:352302/250‑1 (MQ=255)

                                                                                                                                            aTGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:273808/250‑1 (MQ=255)

                                                                                                                                             tGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAccc                                                                                                  <  1:421986/251‑1 (MQ=255)

                                                                                                                                              ggCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTgc                                                                                                                                                                                                     <  1:742383/152‑1 (MQ=255)

                                                                                                                                              ggCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTAc                                                                                                                       >  1:307961/1‑229 (MQ=255)

                                                                                                                                              ggCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAcc                                                                                                   >  1:816442/1‑249 (MQ=255)

                                                                                                                                               gCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTAc                                                                                                                       >  1:26314/1‑228 (MQ=255)

                                                                                                                                                   aaCCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            >  1:860549/1‑251 (MQ=255)

                                                                                                                                                   aaCCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            <  1:814494/251‑1 (MQ=255)

                                                                                                                                                   aaCCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            <  1:394842/251‑1 (MQ=255)

                                                                                                                                                   aaCCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            <  1:202929/251‑1 (MQ=255)

                                                                                                                                                        ccGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAAc                                                                                       <  1:831081/250‑1 (MQ=255)

                                                                                                                                                         cGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACt                                                                                      <  1:104243/251‑1 (MQ=255)

                                                                                                                                                         cGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACt                                                                                      <  1:621243/251‑1 (MQ=255)

                                                                                                                                                         cGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACt                                                                                      <  1:706868/251‑1 (MQ=255)

                                                                                                                                                               cGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCtt                                                                                >  1:441813/1‑251 (MQ=255)

                                                                                                                                                                gCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCttt                                                                               >  1:144196/1‑251 (MQ=255)

                                                                                                                                                                 ccTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTc                                                                                                                                       >  1:136313/1‑194 (MQ=255)

                                                                                                                                                                 ccTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTAc                                                                                                                       >  1:392645/1‑210 (MQ=255)

                                                                                                                                                                    tGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTAttt                                                                           <  1:294566/251‑1 (MQ=255)

                                                                                                                                                                     ggTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AAtt                                                                                                                                        <  1:639021/189‑1 (MQ=255)

                                                                                                                                                                     ggTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTc                                                                                                                                       <  1:578690/190‑1 (MQ=255)

                                                                                                                                                                     ggTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTAtttt                                                                          >  1:188902/1‑251 (MQ=255)

                                                                                                                                                                              cGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCAcg                                                                  >  1:614593/1‑250 (MQ=255)

                                                                                                                                                                                cTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGc                                                                                                                   >  1:873399/1‑199 (MQ=255)

                                                                                                                                                                                cTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCAcgcg                                                                >  1:388157/1‑250 (MQ=255)

                                                                                                                                                                                        gAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGt                                                       >  1:338800/1‑251 (MQ=255)

                                                                                                                                                                                         aatttaatttGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGtt                                                      >  1:322597/1‑251 (MQ=255)

                                                                                                                                                                                         aatttaatttGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGt                                                       <  1:677888/250‑1 (MQ=255)

                                                                                                                                                                                          atttaatttGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAAc                                                                                                                                                            <  1:240885/149‑1 (MQ=255)

                                                                                                                                                                                          atttaatttGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGtt                                                      >  1:640949/1‑250 (MQ=255)

                                                                                                                                                                                          atttaatttGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTc                                                     <  1:178229/251‑1 (MQ=255)

                                                                                                                                                                                                   ggTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATa                                             >  1:270052/1‑250 (MQ=255)

                                                                                                                                                                                                    gTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATac                                            <  1:567448/250‑1 (MQ=255)

                                                                                                                                                                                                         aGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTGAAATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            >  1:824438/1‑198 (MQ=255)

                                                                                                                                                                                                         aGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAAc                                                                                                    <  1:289480/189‑1 (MQ=255)

                                                                                                                                                                                                                   gcaTTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCttatta                                                                                                                        >  1:848923/1‑159 (MQ=255)

                                                                                                                                                                                                                   gcaTTTTCCCGTCAATTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGa                             <  1:170660/250‑1 (MQ=255)

                                                                                                                                                                                                                                aaTTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTgccg               >  1:197205/1‑251 (MQ=255)

                                                                                                                                                                                                                                 aTTTAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAg                                                                                            <  1:569538/173‑1 (MQ=255)

                                                                                                                                                                                                                                  tttAACGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTgccgcc             <  1:575484/251‑1 (MQ=255)

                                                                                                                                                                                                                                      aCGTGCCGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTGCCGCCACgg         <  1:608978/251‑1 (MQ=255)

                                                                                                                                                                                                                                           ccGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGAcg                           <  1:316948/228‑1 (MQ=255)

                                                                                                                                                                                                                                            cGCTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑CATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGAc                            >  1:611932/1‑226 (MQ=255)

                                                                                                                                                                                                                                              cTGCACTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACGTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTGCCGCCACGGCATTTTg  >  1:715698/1‑250 (MQ=255)

AGATCAGGATGAATAATTCATGGATTATGTGTTGGTGGTGAAACAACCGGTTTACGGTAGTCAAGGCGCGTTTCTGGCGTACCAACTGGCACAAGTACTGGTGCAAAAGCATCATATCAGCCAAATCTTTTTTTATCAGGATGGCGTAACCAACGGCAACGCCTTGGTTTATCCCGCTAATGACGAATTTAATTTGGTCCAAGCCTGGCAGGCATTTTCCCGTCAATTTAACGTGCCGCTGCATTTGTGCGTTGCCGCCGCTCAACGGCGTGGCGTGGTGGATAGCTATTCTGCCGCCGATAAAAGTGCGGTTAACCTGGCGGACTTTTTTCAACTTGCCGGCTTGGGTG‑AATTCAGCCAAGCCTTATTACAAGCCGATCGGGTGATAACCCTATGAGTAAACTTGCCTTTATTTTCCGTCACGCGCCTTATGGTTCGGCAAATACACGCGAAGGATTAGACGCGCTGCTTGCCGCCACGGCATTTTG  >  2/140144‑140631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`