Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	134,963	T→C	V271V (GTT→GTC)	unknown →	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	134,963	0	T	C	100.0%	188.6 / NA	70	V271V (GTT→GTC)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/64);  total (6/64)

AAATGAACGATTTCTTTCTCGACAGCATTCGTGGCATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTGCGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTTGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGATTTTGTCCGATGTGAATTTGCAC  >  2/134722‑135209                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          aaaTGAACGATTTCTTTCTCGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAGCGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTa                                                                                                                                                                                                                                                  >  1:885286/1‑251 (MQ=255)  aaTGAACGATTTCTTTCTCGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAg                                                                                                                                                                                                                                                 <  1:367187/251‑1 (MQ=255)      aaCGATTTCTTTCTCGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTgg                                                                                                                                                                                                                                              <  1:456702/250‑1 (MQ=255)      aaCGATTTCTTTCTCGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTgg                                                                                                                                                                                                                                              <  1:613813/250‑1 (MQ=255)                  ctcGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCt                                                                                                                                                                                                                                 <  1:524936/251‑1 (MQ=255)                  ctcGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCt                                                                                                                                                                                                                                 <  1:697239/251‑1 (MQ=255)                  ctcGACAGCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCt                                                                                                                                                                                                                                 <  1:19108/251‑1 (MQ=255)                         gCATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGGttttttttttttt                                                                                                                                                                                                                           >  1:813521/1‑241 (MQ=255)                          cATTCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTc                                                                                                                                                                                                                           <  1:842214/249‑1 (MQ=255)                            ttCGTGGTATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGc                                                                                                                                                                                                                       <  1:719422/251‑1 (MQ=255)                                  gtaTGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGGttttttttttttttcgctttcaa                                                                                                                                                                                                                 >  1:271791/3‑232 (MQ=255)                                  gtaTGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTc                                                                                                                                                                                                                           <  1:384453/239‑1 (MQ=255)                                   taTGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAc                                                                                                                                                                                                                <  1:195803/250‑1 (MQ=255)                                   taTGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAc                                                                                                                                                                                                                <  1:569254/250‑1 (MQ=255)                                   taTGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAc                                                                                                                                                                                                                <  1:153163/250‑1 (MQ=255)                                       aaagaaaTTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCgg                                                                                                                                                                                                             <  1:632946/250‑1 (MQ=255)                                        aagaaaTTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTg                                                                                                                                                                                                           <  1:458287/251‑1 (MQ=255)                                              ttCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGcc                                                                                                                                                                                                     <  1:258399/251‑1 (MQ=255)                                              ttCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGcc                                                                                                                                                                                                     <  1:712580/251‑1 (MQ=255)                                                  gTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGGGGTTTTTCTTTTGTTCCGCTACGGACCgggggttgttttta                                                                                                                                                                                                 >  1:334923/1‑239 (MQ=255)                                                    tATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGt                                                                                                                                                                                               <  1:228408/251‑1 (MQ=255)                                                    tATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGt                                                                                                                                                                                               <  1:602724/251‑1 (MQ=255)                                                    tATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGt                                                                                                                                                                                               <  1:468144/251‑1 (MQ=255)                                                     aTTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTa                                                                                                                                                                                              <  1:821844/251‑1 (MQ=255)                                                          gCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAAtct                                                                                                                                                                                          <  1:158925/250‑1 (MQ=255)                                                             aCGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTcc                                                                                                                                                                                      <  1:206353/251‑1 (MQ=255)                                                               gAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGcc                                                                                                                                                                                                     <  1:244345/234‑1 (MQ=255)                                                               gAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAAtctct                                                                                                                                                                                        <  1:191163/247‑1 (MQ=255)                                                                aaCAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGTATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAAt                                                                                                                                                                                   <  1:475066/251‑1 (MQ=255)                                                                aaCAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAAt                                                                                                                                                                                   <  1:69251/251‑1 (MQ=255)                                                                  cAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATaa                                                                                                                                                                                 <  1:840190/251‑1 (MQ=255)                                                                  cAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATaa                                                                                                                                                                                 <  1:110726/251‑1 (MQ=255)                                                                  cAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTAATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATaa                                                                                                                                                                                 <  1:861069/251‑1 (MQ=255)                                                                   aaaaaCGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAAc                                                                                                                                                                                <  1:657965/251‑1 (MQ=255)                                                                    aaaaCGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAAcc                                                                                                                                                                               <  1:409986/251‑1 (MQ=255)                                                                                  aaaTTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGc                                                                                                                                                                                                                                             <  1:792797/175‑1 (MQ=255)                                                                                  aaaTTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAAcc                                                                                                                                                                               <  1:516621/237‑1 (MQ=255)                                                                                     ttCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAc                                                                                                                                                                                                                <  1:22971/201‑1 (MQ=255)                                                                                      tCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCa                                                                                                                                                            <  1:35476/251‑1 (MQ=255)                                                                                          cAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAgcgg                                                                                                                                                        <  1:783501/251‑1 (MQ=255)                                                                                                    aaaaaaTCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTa                                                                                                                                              <  1:456909/251‑1 (MQ=255)                                                                                                          tCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCg                                                                                                                                        <  1:73680/251‑1 (MQ=255)                                                                                                          tCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCg                                                                                                                                        <  1:821687/251‑1 (MQ=255)                                                                                                              tCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGc                                                                                                                                    <  1:718379/251‑1 (MQ=255)                                                                                                                       cTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGt                                                                                                                                                                                               <  1:667142/184‑1 (MQ=255)                                                                                                                       cTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAAcc                                                                                                                                                                               <  1:425545/200‑1 (MQ=255)                                                                                                                       cTTAAAAATCAAACAACAGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAAc                                                                                                                           <  1:424401/251‑1 (MQ=255)                                                                                                                                    caacaGGAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGt                                                                                                                                                                                               <  1:421987/171‑1 (MQ=255)                                                                                                                                          gAAGGCAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGa                                                                                                           <  1:549694/248‑1 (MQ=255)                                                                                                                                               cAATGTACGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGaa                                                                                                    <  1:530209/250‑1 (MQ=255)                                                                                                                                                   gtacGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATaa                                                                                                                                                                                 <  1:664295/167‑1 (MQ=255)                                                                                                                                                    tacGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATgg                                                                                               <  1:547478/248‑1 (MQ=255)                                                                                                                                                          ccTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGc                                                                                                                                         <  1:820793/202‑1 (MQ=255)                                                                                                                                                           cTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGagtaagta                                                                                       <  1:592713/251‑1 (MQ=255)                                                                                                                                                            tttACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTAt                                                                                      <  1:789896/251‑1 (MQ=255)                                                                                                                                                                cGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑cc                                                                                 <  1:783459/251‑1 (MQ=255)                                                                                                                                                                  gAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAAt                                                                                                 <  1:627798/234‑1 (MQ=255)                                                                                                                                                                                    tttAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGt                                                            <  1:668408/251‑1 (MQ=255)                                                                                                                                                                                          atCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGaaaa                                                      <  1:143009/251‑1 (MQ=255)                                                                                                                                                                                              tGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTc                                                  <  1:334437/251‑1 (MQ=255)                                                                                                                                                                                                aTTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTTTTTTTTTTCGGCTACGGGCCCGTGGTTGTCTTTAGTAATATCTCCCATAAACCGCTGC‑AAACCCCTGCCCGCGGCGGGGGGGGATTTGGGGTGCTGGCGGGACAGCCCGCATTTAAAGAAGGGGGAAAAGGAGGAAGGATCA‑CCCAGGTGGA‑GCAAAATaaagctgtctcttttat                                                >  1:102736/1‑236 (MQ=255)                                                                                                                                                                                                 ttttACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAc                                                                                                                                                                                                                <  1:824342/93‑1 (MQ=255)                                                                                                                                                                                                   ttACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGttt                                             <  1:780841/251‑1 (MQ=255)                                                                                                                                                                                                         caccagctcgcggtATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTa                                       <  1:645321/238‑1 (MQ=255)                                                                                                                                                                                                            ggCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTTTTTTTGTTTGGCTACGGGCCGGTGGTTTCCCTTAGGAATTTCTCCCATAAACTTCTGCAAAACCCTT‑CCCGCGGCGAGGGGGGGTTTGCGGTGCTGGGGGGACAGCCCGAAATGGAAAAAGTGGGAAATGGGGGGAGTATTACCCGA‑GTGCAGGCAAATTAAggaggaaaaggtaggtttttttttgt                                     >  1:791539/1‑227 (MQ=255)                                                                                                                                                                                                            ggCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATc                                                                                     <  1:522840/204‑1 (MQ=255)                                                                                                                                                                                                                         ttGCTTATGATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGAtt                       <  1:814474/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATAAAATTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCGTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGAt                        <  1:442765/242‑1 (MQ=255)                                                                                                                                                                                                                                      aaTTGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGAcc                                                                                                                                                                                                               <  1:618054/57‑1 (MQ=255)                                                                                                                                                                                                                                         tGATTTTGTCGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGATTTTGTCCGATGTGAAtt       <  1:878418/251‑1 (MQ=255)                                                                                                                                                                                                                                              ttgtcgGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGATTTTGTCCGATGTGAATTTGcac  <  1:271330/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          AAATGAACGATTTCTTTCTCGACAGCATTCGTGGCATGAAAGAAATTCAGTTATTCGGCAACGAACAAAAACGCCTACAGGAAATTCACCAACGCAGCGAAAAAATCGATCAGGCATTCTTAAAAATCAAACAACAGGAAGGCAATGTGCGTTCCTTTACGGAAATCGCCGTATCGTTTTTTAATATCGTGATTTTACTGGTGGGCTTAACGTTATTTGCTTATGATAAAATTGATTTTGTTGGCTTGTTAGTGGCGGTGATTTTGCTTATGTCCGGCTACGGACCGGTGATTGCCTTAAGTAATCTCTCCAATAACCTGCTGC‑AAACCCTTGCCAGCGGCGAGCGGGTATTGGCGTTGCTGGCGGAACAGCCTGAATTGAAAGACGTGGAAAATGGAGTAAGTATCA‑CCGATGTGCA‑GCAAATTAAGGTAGAAAATGTCAGTTTTGCTTATGCGCAGGAACAGATTTTGTCCGATGTGAATTTGCAC  >  2/134722‑135209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑