Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	133,981	G→A	A532A (GCG→GCA)	unknown →	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	133,981	0	G	A	100.0%	251.1 / NA	87	A532A (GCG→GCA)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/35);  total (52/35)

GGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCGGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGATCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTATGGGCAC  >  2/133735‑134218                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              ggCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGa                                                                                                                                                                                                                                           >  1:59032/1‑251 (MQ=255)  gCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGa                                                                                                                                                                                                                                           <  1:133840/250‑1 (MQ=255)  gCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGa                                                                                                                                                                                                                                           <  1:278339/250‑1 (MQ=255)                   aaaCGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTAtg                                                                                                                                                                                                                         <  1:686505/251‑1 (MQ=255)                    aaCGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTAtgt                                                                                                                                                                                                                        <  1:776301/251‑1 (MQ=255)                    aaCGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTAtgt                                                                                                                                                                                                                        <  1:734746/251‑1 (MQ=255)                        gcggTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCGATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTa                                                                                                                                                                                                                    >  1:575974/1‑251 (MQ=255)                           gTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACaa                                                                                                                                                                                                                 >  1:474237/1‑251 (MQ=255)                             ttGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAg                                                                                                                                                                                                               <  1:692371/251‑1 (MQ=255)                                gagaTGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCgg                                                                                                                                                                                                            >  1:100001/1‑251 (MQ=255)                                 agaTGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCgg                                                                                                                                                                                                            <  1:287716/250‑1 (MQ=255)                                        gTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTAtgt                                                                                                                                                                                                                        >  1:325248/1‑231 (MQ=255)                                                                      cggGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATgg                                                                                                                                                                      <  1:405420/251‑1 (MQ=255)                                                                      cggGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATgg                                                                                                                                                                      <  1:651077/251‑1 (MQ=255)                                                                          cAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGa                                                                                                                                                                  >  1:88535/1‑251 (MQ=255)                                                                             aTTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGaaaa                                                                                                                                                               >  1:643000/1‑251 (MQ=255)                                                                                    gTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAAtt                                                                                                                                                        >  1:163231/1‑251 (MQ=255)                                                                                                    cgcCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtc                                                                                                                                         <  1:275147/250‑1 (MQ=255)                                                                                                      cccTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGAt                                                                                                                                                                                                                              <  1:85386/163‑1 (MQ=255)                                                                                                      cccTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtcaat                                                                                                                                      >  1:222290/1‑251 (MQ=255)                                                                                                      cccTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtcaat                                                                                                                                      >  1:877925/1‑251 (MQ=255)                                                                                                        cTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAAt                                                                                                                                                         <  1:715980/230‑1 (MQ=255)                                                                                                        cTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtcaatca                                                                                                                                    >  1:55747/1‑251 (MQ=255)                                                                                                        cTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtcaatca                                                                                                                                    >  1:468610/1‑251 (MQ=255)                                                                                                             gCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATc                                                                                                                                                                                             >  1:789994/1‑189 (MQ=255)                                                                                                             gCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATg                                                                                                                                                    <  1:504570/230‑1 (MQ=255)                                                                                                             gCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACaaa                                                                                                                               >  1:535170/1‑251 (MQ=255)                                                                                                             gCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACaaa                                                                                                                               >  1:826677/1‑251 (MQ=255)                                                                                                                  aCGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAg                                                                                                                             >  1:739211/1‑248 (MQ=255)                                                                                                                  aCGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAAt                                                                                                                          >  1:832613/1‑251 (MQ=255)                                                                                                                  aCGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAAt                                                                                                                          >  1:519455/1‑251 (MQ=255)                                                                                                                  aCGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAAt                                                                                                                          >  1:431476/1‑251 (MQ=255)                                                                                                                  aCGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAAt                                                                                                                          >  1:417519/1‑251 (MQ=255)                                                                                                                   cGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGc                                                                                                                                                   <  1:749918/225‑1 (MQ=255)                                                                                                                     cTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAAttt                                                                                                                        <  1:398504/250‑1 (MQ=255)                                                                                                                        gtGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGaaa                                                                                                                                              >  1:867425/2‑225 (MQ=255)                                                                                                                          gTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGaaaa                                                                                                                  >  1:667618/1‑251 (MQ=255)                                                                                                                            cTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAg                                                                                                                             <  1:25257/238‑1 (MQ=255)                                                                                                                                            gCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTtc                                                                                                                                         <  1:696354/210‑1 (MQ=255)                                                                                                                                            gCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCaaa                                                                                                >  1:804227/1‑251 (MQ=255)                                                                                                                                              tACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATc                                                                                              <  1:862369/251‑1 (MQ=255)                                                                                                                                                ccAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAAt                                                                                                                                                         >  1:471980/1‑190 (MQ=255)                                                                                                                                                       cATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAg                                                                                     >  1:246922/1‑251 (MQ=255)                                                                                                                                                           gATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACg                                                                                 >  1:197518/1‑251 (MQ=255)                                                                                                                                                            aTGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGaa                                                                                                          >  1:249510/1‑225 (MQ=255)                                                                                                                                                            aTGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACgg                                                                                >  1:626324/1‑251 (MQ=255)                                                                                                                                                              gTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGAt                                                                              >  1:714416/1‑251 (MQ=255)                                                                                                                                                                          gaaATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCAtgt                                                                  >  1:246756/1‑251 (MQ=255)                                                                                                                                                                                       aaTTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCa                                                                                             >  1:239551/1‑211 (MQ=255)                                                                                                                                                                                        aTTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGaaa                                                                                                                                              <  1:302694/161‑1 (MQ=255)                                                                                                                                                                                        aTTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGaaa                                                    >  1:431412/1‑251 (MQ=255)                                                                                                                                                                                         tttATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGc                                                               <  1:574872/239‑1 (MQ=255)                                                                                                                                                                                          ttATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCACCAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCg                                                                                         >  1:439053/1‑212 (MQ=255)                                                                                                                                                                                          ttATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATc                                                                                                              >  1:833515/1‑191 (MQ=255)                                                                                                                                                                                          ttATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATg                                                                                           <  1:664241/210‑1 (MQ=255)                                                                                                                                                                                          ttATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAAtt                                                  >  1:195935/1‑251 (MQ=255)                                                                                                                                                                                            aTTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCAtg                                                                   >  1:481276/1‑232 (MQ=255)                                                                                                                                                                                            aTTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTgg                                                >  1:222261/1‑251 (MQ=255)                                                                                                                                                                                            aTTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTgg                                                >  1:75410/1‑251 (MQ=255)                                                                                                                                                                                                   aGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCg                                                                                         <  1:846912/203‑1 (MQ=255)                                                                                                                                                                                                   aGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGc                                                               >  1:35804/1‑229 (MQ=255)                                                                                                                                                                                                    gCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATggggg                                                                                                    <  1:204583/191‑1 (MQ=255)                                                                                                                                                                                                    gCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCg                                        >  1:304558/1‑251 (MQ=255)                                                                                                                                                                                                    gCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCg                                        >  1:314109/1‑251 (MQ=255)                                                                                                                                                                                                    gCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCg                                        >  1:766145/1‑251 (MQ=255)                                                                                                                                                                                                     cTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTgtcg                                                                        >  1:297815/1‑218 (MQ=255)                                                                                                                                                                                                     cTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGt                                       >  1:28101/1‑251 (MQ=255)                                                                                                                                                                                                          acacAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGcc                                  <  1:564181/251‑1 (MQ=255)                                                                                                                                                                                                             caGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGc                                                                                                                                                   >  1:244618/1‑135 (MQ=255)                                                                                                                                                                                                             caGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATggggg                                                                                                    <  1:532879/182‑1 (MQ=255)                                                                                                                                                                                                                  aaaCCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGaa                                                                                                          >  1:562202/1‑171 (MQ=255)                                                                                                                                                                                                                  aaaCCATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCAcatcat                          >  1:805382/1‑251 (MQ=255)                                                                                                                                                                                                                     ccATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATg                                                                                                        >  1:800026/1‑170 (MQ=255)                                                                                                                                                                                                                     ccATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAg                       <  1:54373/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAg                       <  1:813975/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATTGTGATGATTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATAGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAg                       >  1:455872/1‑251 (MQ=255)                                                                                                                                                                                                                             atgatTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCAcc                <  1:375213/250‑1 (MQ=255)                                                                                                                                                                                                                             atgatTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACca               <  1:889782/251‑1 (MQ=255)                                                                                                                                                                                                                               gatTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATggggg                                                                                                    >  1:259545/1‑164 (MQ=255)                                                                                                                                                                                                                                atTTCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACcatc             <  1:548344/250‑1 (MQ=255)                                                                                                                                                                                                                                 tttCTCATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACcatcat           >  1:743218/1‑251 (MQ=255)                                                                                                                                                                                                                                      cATCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTa         <  1:828405/248‑1 (MQ=255)                                                                                                                                                                                                                                       aTCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTATggg     <  1:807615/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTATggg     <  1:659239/251‑1 (MQ=255)                                                                                                                                                                                                                                        tCGGTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAAGTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCGCATCATGAGTTTCACCATCATTATGGGc    <  1:835143/251‑1 (MQ=255)                                                                                                                                                                                                                                           gTTGGCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTATGGGCAc  >  1:658378/1‑250 (MQ=255)                                                                                                                                                                                                                                              ggCAAACGCAGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGACCATGCCTC‑TTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAg                       <  1:183667/225‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATGTCTATATTTTCGATGAAGCTACCAGTAACATCGATGTAGAAAGCGAAGAAATTATTCTGCAATTTATTCAACAGCTTAAACACAGCAAAACCATTGTGATGATTTCTCATCGGTTGGCAAACGCGGTGAACGCGGATCAGATTTATGTGCTACAAAGCGGTCGCTTGATGGAATCCGGCGATCATGCCTCTTTAATGGCAGAAAACGGAATTTATGCAGAAATGTTCAATCAACAAAAGAATTTAGAAAATATCAGAATGGGGGCAAATCATGCGTAAGAACGGATTTGTCGTCATGTGGCAGCTCATGAAATTGGTTACGCCGTTAGCCCACATCATGAGTTTCACCATCATTATGGGCAC  >  2/133735‑134218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑