Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	133,615	C→A	H410Q (CAC→CAA)	unknown →	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	133,615	0	C	A	100.0%	179.8 / NA	64	H410Q (CAC→CAA)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/25);  total (39/25)

AAAATGAAGTCACCGTGCAAATTAACGATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCACATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATG  >  2/133374‑133856                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  aaaaTGAAGTCACCGTGCAAATTAACGATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCg                                                                                                                                                                                                                                             >  1:685890/1‑248 (MQ=255)               gTGCAAATTAACGATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCAcagcag                                                                                                                                                                                                                            >  1:625662/1‑251 (MQ=255)                    aaTTAACGATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGGATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACa                                                                                                                                                                                                                       >  1:580783/1‑251 (MQ=255)                           gATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACAt                                                                                                                                                                                                                      >  1:9681/1‑245 (MQ=255)                                       gCCTATTCCGAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACa                                                                                                                                                                                                                       >  1:165738/1‑232 (MQ=255)                                                gAGGAAAAACCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGagataa                                                                                                                                                                                            >  1:61177/1‑245 (MQ=255)                                                                ttAACGGCTTACCCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa                                                                                                                                                                           <  1:60444/251‑1 (MQ=255)                                                                ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAAGGGTTTTGGTGGGCAAAAGCGGTTGTGGAAAATCGACCTTGGTATCTTTCCTAATGGGGTTCTATCAGGCGCAACAAGGCAAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa                                                                                                                                                                           <  1:503245/251‑1 (MQ=255)                                                                ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa                                                                                                                                                                           <  1:343401/251‑1 (MQ=255)                                                                ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa                                                                                                                                                                           <  1:290315/251‑1 (MQ=255)                                                                  aacggCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCaaa                                                                                                                                                                         <  1:220155/248‑1 (MQ=255)                                                                     ggCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATg                                                                                                                                                                       >  1:616928/1‑250 (MQ=255)                                                                      gCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCggg                                                                                                                                                                                                  >  1:267297/1‑222 (MQ=255)                                                                      gCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCCTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAAt                                                                                                                                                                        <  1:546808/248‑1 (MQ=255)                                                                           aCCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATgg                                                                                                                                                                                   <  1:797892/232‑1 (MQ=255)                                                                             ccTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCcgacg                                                                                                                                                              >  1:360123/1‑251 (MQ=255)                                                                                          cagAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGAAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTAt                                                                                                                                                   <  1:387173/247‑1 (MQ=255)                                                                                                 aGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa                                                                                                                                                                           >  1:355211/1‑218 (MQ=255)                                                                                                  gTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGc                                                                                                                                                                                                            >  1:387901/1‑184 (MQ=255)                                                                                                                 gggCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTgg                                                                                                                          >  1:551840/1‑251 (MQ=255)                                                                                                                      aaaGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCAAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATc                                                                                                                                                  <  1:359428/222‑1 (MQ=255)                                                                                                                       aaGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAAtt                                                                                                                    >  1:81513/1‑251 (MQ=255)                                                                                                                            gTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGgtg                                                                                                                                                                                 <  1:764948/185‑1 (MQ=255)                                                                                                                                          aCCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAAt                                                                                                                              <  1:893997/222‑1 (MQ=255)                                                                                                                                            cTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGGGAAATTTTGTTTAACGGCATCGATATTAACCAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCcgacg                                                                                                                                                              <  1:228601/188‑1 (MQ=255)                                                                                                                                                gTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAAttt                                                                                                                   <  1:572667/227‑1 (MQ=255)                                                                                                                                                 tATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGgag                                                                                          <  1:184770/251‑1 (MQ=255)                                                                                                                                                  aTCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGgaga                                                                                         <  1:732553/251‑1 (MQ=255)                                                                                                                                                    cTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGa                                                                                                                                        <  1:688804/202‑1 (MQ=255)                                                                                                                                                    cTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATg                                                                                       >  1:162948/1‑251 (MQ=255)                                                                                                                                                    cTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATg                                                                                       >  1:549906/1‑251 (MQ=255)                                                                                                                                                      ttACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAAc                                                                                                                                      >  1:731796/1‑202 (MQ=255)                                                                                                                                                      ttACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAAcggc                                                                                                   >  1:96885/1‑237 (MQ=255)                                                                                                                                                               gggTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAg                                                                            >  1:680915/1‑251 (MQ=255)                                                                                                                                                                 gTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGt                                                                           >  1:392719/1‑250 (MQ=255)                                                                                                                                                                                               gTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAAtt                                             >  1:742587/1‑250 (MQ=255)                                                                                                                                                                                                     cGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGaa                                                                                                         >  1:472549/1‑184 (MQ=255)                                                                                                                                                                                                       gCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTa                                    >  1:333931/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAg                                                                                                                                                           >  1:782670/1‑126 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGgag                                                                                          >  1:724529/1‑191 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACg                                        >  1:415274/1‑241 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCtt                              >  1:696818/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCtt                              >  1:211263/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCtt                              >  1:45191/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGGTTAGCGCtt                              >  1:795849/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGAGaa                                                                                                         <  1:252301/176‑1 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGCGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCtt                              >  1:347158/1‑251 (MQ=255)                                                                                                                                                                                                             atatTAAACAGATTAACCGCCATTCCTTATATCCCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGCCAGCTTGCGGGAAACTATGCTAATGGAGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGCTTGGAGAAGCCGACGCTCATCCGCGGAAGCAATTTTGCCGGCGGGCAAATTCAACGATTAACGCtt                              >  1:263113/1‑251 (MQ=255)                                                                                                                                                                                                                       gATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAAc                                                                                                                                      <  1:781729/137‑1 (MQ=255)                                                                                                                                                                                                                       gATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACg                                        >  1:336250/1‑231 (MQ=255)                                                                                                                                                                                                                             ccGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTg               >  1:216007/1‑250 (MQ=255)                                                                                                                                                                                                                             ccGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGc              <  1:535369/251‑1 (MQ=255)                                                                                                                                                                                                                                ccATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCAt            <  1:581391/250‑1 (MQ=255)                                                                                                                                                                                                                                ccATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCAt            <  1:661713/250‑1 (MQ=255)                                                                                                                                                                                                                                ccATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCAt            <  1:111644/250‑1 (MQ=255)                                                                                                                                                                                                                                ccATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATa           >  1:893511/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGc                                                                                      <  1:516381/174‑1 (MQ=255)                                                                                                                                                                                                                                    tCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACg        >  1:708949/1‑250 (MQ=255)                                                                                                                                                                                                                                    tCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACg        >  1:449114/1‑250 (MQ=255)                                                                                                                                                                                                                                    tCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGc       <  1:237166/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGCTTAGCGCTTGCCCGCGCCCTTTTGCATAACg        >  1:338501/1‑250 (MQ=255)                                                                                                                                                                                                                                     ccTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACg        >  1:695526/1‑249 (MQ=255)                                                                                                                                                                                                                                     ccTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATACCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCAt            >  1:432241/1‑245 (MQ=255)                                                                                                                                                                                                                                         atatCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATg  <  1:782960/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  AAAATGAAGTCACCGTGCAAATTAACGATTTACATTTTGCCTATTCCGAGGAAAAACCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCACATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCAAATGCCTCCGACGAAGTCATTTATCAATGTTTGGAACAAGTGAATCTGGCGAATTTTGTGCGGGAAAACGGCGGTTTGGAGATGCCGTTGCTCAGTCGCGGAAGCAATTTGTCCGGCGGGCAAATTCAACGTTTAGCGCTTGCCCGCGCCCTTTTGCATAACGCTGATG  >  2/133374‑133856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑