BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	133,441	A→C	K352N (AAA→AAC)	unknown →	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	133,441	0	A	C	100.0%	171.2 / NA	63	K352N (AAA→AAC)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (37/26); total (37/26)

TTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTATGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCACATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCA  >  2/133203‑133687

ttttCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCt                                                                                                                                                                                                                                             >  1:461499/1‑250 (MQ=255)

           gtgtTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGc                                                                                                                                                                                                                                  >  1:99119/1‑250 (MQ=255)

                 tttATTTTACTGGCTTCAGATTTTTTCATTCCATGGCGCTTG‑TTGGTTTCTTTCTCCGGTGGGGGGAGAAAGGGCAAGGC‑GGTTGGGAAAAAAATCTTTACGCTGTTGAATAC‑GCGGGTGGAA‑AAAATAAAGGGGGGGTGGATTTCCCGGTT‑AAAAATA‑AAGTCACCGT‑GAAAATAAACG‑TTTTCCTTTTTG‑C‑CTTTTC‑A‑G‑GGAAAAACCCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAg                                                                                                                                                                                                                          <  1:205256/251‑1 (MQ=255)

                    aTTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGtt                                                                                                                                                                                                                        >  1:865289/1‑251 (MQ=255)

                     ttttACTGGCTTCCGAATTTTTCTTCCCATGGCTCTTTCTTGG‑TTCTTTCTGCCAGGTGGCGATGAACGGCAAAGCGGCTGCGG‑AAAAAATCTTAACGCGGTTGAATAG‑GCCGGTGGAAAAAAATAAAAGTGCGGT‑GATTTTTCGGAT‑AAAAAAGAAAGCCCCCGT‑CCAAATAAACT‑ATTTACATTTTG‑C‑CTATCC‑C‑AAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTa                                                                                                                                                                                                                       <  1:379935/251‑1 (MQ=255)

                       ttACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTaa                                                                                                                                                                                                                      >  1:499263/1‑250 (MQ=255)

                              cTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCAt                                                                                                                                                                                                                                       >  1:731589/1‑226 (MQ=255)

                                         ttCATCCCATTGCGTTTGTTTGT‑TTCCTTCTTCCATGTGGCGATGAACGGAAAAGCTGCTTAGA‑AAAAATTCTTTACGTTGTTGGA‑ACGGCGGGTGAAAAAAAATAAAAGTGCGGTGGTTTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑TTTTAC‑TTTTGAC‑CTATCC‑C‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAg                                                                                                                                                                                                   <  1:834088/251‑1 (MQ=255)

                                                         tgcttgG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCg                                                                                                                                                                                   >  1:624516/1‑251 (MQ=255)

                                                          gcttgG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGa                                                                                                                                                                                  >  1:283042/1‑251 (MQ=255)

                                                               g‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                                                                                                                                                                   >  1:894449/1‑213 (MQ=255)

                                                                  tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                                                                                                                                                                   >  1:503100/1‑211 (MQ=255)

                                                                  tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                                                                                                                                                           >  1:781522/1‑251 (MQ=255)

                                                                  tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                                                                                                                                                           >  1:7662/1‑251 (MQ=255)

                                                                  tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTACTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                                                                                                                                                           >  1:588155/1‑251 (MQ=255)

                                                                   ctttctccCATGGGGCGATGAGCGGAAAAGCTGCTTGGG‑AAAAAATTTTTCCGTTGTTGGATAG‑GCGGGTGGAAAAAAAAAAAAGGGGGGTGGTTTTTCCGTTT‑AAAAAGG‑AATTCACCGT‑AAAAATTACCG‑TTTTACTTTTTG‑C‑CTATCC‑C‑GTGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTa                                                                                                                                                                          <  1:619018/249‑1 (MQ=255)

                                                                     ttctcccgtggggCGATGACCGGCAAAGCTGTTTCGA‑AAAAAATCTTTACGCTTTTGGATAC‑GCCGGTGGAAAAAAAAAAAAGTGCGGTGTATTTTCCGGTA‑AAAAATG‑AAGCCCCCGT‑GAAAATTACCG‑‑TTTTCCTTTTGCC‑CTATCC‑C‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATc                                                                                                                                                                        <  1:508853/240‑1 (MQ=255)

                                                                      tcttcCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTAt                                                                                                                                                                         >  1:897044/1‑249 (MQ=255)

                                                                        ttcCATGTGGCGATGAACGGCAAAGCGGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAAAAAAAGTGCGGTGGATTTTCCGGTA‑AAAAAGG‑AAGTCCCCGT‑GAAAATTAACT‑TTTTACATTTTG‑C‑CTATTC‑C‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCttt                                                                                                                                                                     <  1:228891/251‑1 (MQ=255)

                                                                         tcCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTa                                                                                                                                                                    >  1:602651/1‑251 (MQ=255)

                                                                          ccttgtgGCGATGAACGGAAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAAAAAAAGTGGGGTGTATTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GCAAATAAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGt                                                                                                                                                                                                                  <  1:224303/201‑1 (MQ=255)

                                                                                ggCGATGACCGGCAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAATAAAAGTGCGGTGGATTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑ATTTACATTTTG‑C‑TTATCC‑C‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATg                                                                                                                                                              <  1:52825/250‑1 (MQ=255)

                                                                                 gCGATGAACGGCAAAGTTGCTTGGA‑AAAAAATCTTTACGTTGTGGGATAC‑GCGGGTGGAAAAAAAAAAAAGGGCGGTGATTTTTCCGGTA‑AAAAATG‑AAGTCACCGT‑GAAAATTAACG‑TTTTACTTTTTC‑C‑CTATCC‑C‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgg                                                                                                                                                             <  1:666532/250‑1 (MQ=255)

                                                                                   gATGAACGACAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAGAAAAAAAAAGGGGGGTGG‑TTTTCCCGTTAAAAAATG‑AAGTCGCCGT‑GCAAATTAACG‑ATTTACTTTTTG‑C‑TTATTC‑C‑GGGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgggg                                                                                                                                                           <  1:88408/250‑1 (MQ=255)

                                                                                   cgatgaaggaaaaagcgtctgcgaaaaaaaTCTTTACGCTGTTGGAGAC‑GCCGGGGAAAAAAAAAAAAAGTGGGGTGGATTTTCCGTTA‑AAAAATG‑AATTCCCCTG‑GCAAATT‑ACGAATTTCAATTTTG‑C‑CT‑TTC‑CAAAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgggg                                                                                                                                                           <  1:760872/227‑1 (MQ=255)

                                                                                         cGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt                                                                                                                                                     >  1:874426/1‑250 (MQ=255)

                                                                                         cGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt                                                                                                                                                     >  1:161164/1‑250 (MQ=255)

                                                                                         acggcaagtttacttagaaaaaaaTCTTCGCGCTGTGGGATAC‑GCGGGTGGAAAAAAATAAGTGTGTGAGAGATTTTCCATTA‑AAAAATG‑AGGCCA‑CGTAGCAAATTAACT‑AATAAC‑TTTTG‑CACTATAC‑A‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt                                                                                                                                                     <  1:893479/233‑1 (MQ=255)

                                                                                           gCAAAGCTGCTTGGA‑AAAAAATCTTTACGCTGTTGGAGAC‑GCGGGTGAAAAAAAATAAAAGGGCGGTGGATTTTCGGGTA‑AAAAAGG‑AAGTCACCGT‑GCAAATTAACT‑ATTTACATTTTC‑C‑CTATTC‑G‑GTGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCa                                                                                                                                                   <  1:575213/250‑1 (MQ=255)

                                                                                           gCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                                                                                                                                                                   >  1:460582/1‑186 (MQ=255)

                                                                                             aaaGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGAAAAAAAAAAAAAGTGGGGTGTATTTTCCGTTA‑AAAAATG‑AAGCCCCCGT‑ACAAATTAACG‑ATTTACATTTTC‑C‑CTATCC‑C‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGgc                                                                                                                                                <  1:794951/251‑1 (MQ=255)

                                                                                                  tgctTCGG‑AAAAATTCTTTGCGTTGTTGGATCC‑GCCGGTGAAAGAAAAAAAAAGGGGGGTGGTTTTTCCGTTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑TTTTCCTTTTTG‑C‑CTATCC‑C‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGcaac                                                                                                                                           <  1:238786/251‑1 (MQ=255)

                                                                                                    tttCGA‑AAAAAATCTTTACGCTTTGGGATCC‑GCGGGTGGAAAAAAATAAAAGTGGGGTGGATTTTCCGGTA‑AAAAATA‑AAGCCCCCGT‑GAAAATTAACG‑ATTTACTTTTTC‑C‑CTATCC‑C‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGcaacaa                                                                                                                                         <  1:344681/250‑1 (MQ=255)

                                                                                                                      acgCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGc                                                                                                                                                                                         >  1:577342/3‑186 (MQ=255)

                                                                                                                         cTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCa                                                                                                                                                                                        >  1:563169/1‑184 (MQ=255)

                                                                                                                         cTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACgg                                                                                                                     >  1:805413/1‑251 (MQ=255)

                                                                                                                         cTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACgg                                                                                                                     >  1:856339/1‑251 (MQ=255)

                                                                                                                              ggATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACgg                                                                                                                     >  1:233255/1‑246 (MQ=255)

                                                                                                                              ggATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCg                                                                                                                >  1:289129/1‑251 (MQ=255)

                                                                                                                                   c‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGgc                                                                                                                                                >  1:513927/1‑214 (MQ=255)

                                                                                                                                         gtgaaaaaaaaaaaaAGTGGGGTGTATTTCCCGGTA‑AAAAATA‑AAGTCCCCGT‑ACAAATTAACG‑ATTTACTTTTTG‑C‑CTA‑TC‑C‑AAAGAAAAACCCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       <  1:601446/243‑1 (MQ=255)

                                                                                                                                         gtgaaaaaaaaTAAAAGTGCGGTGGTTTTTCCGGTA‑AAAAATG‑AAGCCACCGT‑GCAATTTAACT‑ATTTACATTTTC‑C‑CT‑TTCAC‑GAGGAAACC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       <  1:621910/243‑1 (MQ=255)

                                                                                                                                         gtgaaaaaaaaTAAAAGTGCGGTGGATTTTCCGGTA‑AAAAATG‑AAGTCACCGT‑GAAAATTAACG‑ATTTACATTTTC‑C‑CTATCC‑C‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       <  1:243364/243‑1 (MQ=255)

                                                                                                                                         gggaaaaaaaaaaaaAGTGCGGTGGATTTTCCGGTA‑AAAAATG‑AAGCCACCGT‑GAAAATTAACG‑ATTTACTTTTTG‑C‑CTATCC‑C‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       <  1:88891/243‑1 (MQ=255)

                                                                                                                                         gTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCACATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACa                                                                                                      >  1:446525/1‑251 (MQ=255)

                                                                                                                                         gTGGAAAAAAAAAAAAGGGCGGTGGTTTTTCCGGTA‑AAAAATG‑AAGCCCCCGT‑GAAAATTACCG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       <  1:38601/250‑1 (MQ=255)

                                                                                                                                                aaaaataatgtttttggtgtttggcGGTA‑AAAAATG‑AAGCCCCCGT‑GAAAATAAACT‑TTTTACATTTTG‑C‑TTATCC‑G‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAAc                                                                                               <  1:573303/227‑1 (MQ=255)

                                                                                                                                                aaaaTAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTaa                                                                                                >  1:850547/1‑250 (MQ=255)

                                                                                                                                                 aaaTAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTaa                                                                                                                                                                >  1:384601/1‑185 (MQ=255)

                                                                                                                                                 aaaTAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       >  1:416819/1‑242 (MQ=255)

                                                                                                                                                                               aaaaTG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCg                                                                    >  1:685890/1‑248 (MQ=255)

                                                                                                                                                                                              gT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCAcagcag                                                   >  1:625662/1‑251 (MQ=255)

                                                                                                                                                                                                    aaTTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGGATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACa                                              >  1:580783/1‑251 (MQ=255)

                                                                                                                                                                                                         aCG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTatat                                                                                >  1:647707/1‑212 (MQ=255)

                                                                                                                                                                                                           g‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACAt                                             >  1:9681/1‑245 (MQ=255)

                                                                                                                                                                                                                       tG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTatat                                                                                >  1:390493/1‑199 (MQ=255)

                                                                                                                                                                                                                        g‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACa                                              >  1:165738/1‑232 (MQ=255)

                                                                                                                                                                                                                                atattaaacagattaaccGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAAc                                                                                                       >  1:684269/17‑172 (MQ=255)

                                                                                                                                                                                                                                     gAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGagataa                   >  1:61177/1‑245 (MQ=255)

                                                                                                                                                                                                                                                      ttAACGGCTTACCCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa  <  1:60444/251‑1 (MQ=255)

                                                                                                                                                                                                                                                      ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAAGGGTTTTGGTGGGCAAAAGCGGTTGTGGAAAATCGACCTTGGTATCTTTCCTAATGGGGTTCTATCAGGCGCAACAAGGCAAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa  <  1:503245/251‑1 (MQ=255)

                                                                                                                                                                                                                                                      ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa  <  1:343401/251‑1 (MQ=255)

                                                                                                                                                                                                                                                      ttAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCAAATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCa  <  1:290315/251‑1 (MQ=255)

TTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTATGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTC‑C‑GAGGAAAAA‑CCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAAGGCGAAATTTTGTTTAACGGCATCGATATTAAACAGATTAACCGCCATTCCTTATATCGCCACATTTCGCTGGTAAGTCACAGCAGTTACATTTTCAAAGGCAGCTTGCGGGAAAATATGCTAATGGTGTTATCA  >  2/133203‑133687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`