Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	133,320	T→C	M312T (ATG→ACG)	unknown →	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	133,320	0	T	C	100.0%	259.8 / NA	97	M312T (ATG→ACG)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/38);  total (59/38)

CTATTACCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTATGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAA  >  2/133077‑133552                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cTATTACCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTg                                                                                                                                                                                                                                            >  1:331767/1‑251 (MQ=255) cTATTACCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTg                                                                                                                                                                                                                                            >  1:246747/1‑251 (MQ=255)   aTTACCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGa                                                                                                                                                                                                                                          >  1:366139/1‑251 (MQ=255)      aCCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATa                                                                                                                                                                                                                                        >  1:57656/1‑250 (MQ=255)      aCCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       <  1:46653/251‑1 (MQ=255)       ccATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGAt                                                                                                                                                                                                                                         >  1:813050/1‑248 (MQ=255)       ccATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑g                                                                                                                                                                                                                                     >  1:100836/1‑251 (MQ=255)       ccATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑‑AAAAATCTTTACGCTGTTGGATAC‑g                                                                                                                                                                                                                                     >  1:662421/1‑250 (MQ=255)         aTGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑Gcc                                                                                                                                                                                                                                   <  1:468152/251‑1 (MQ=255)         aTGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑Gcc                                                                                                                                                                                                                                   <  1:670566/251‑1 (MQ=255)            aaaGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCggg                                                                                                                                                                                                                                >  1:678459/1‑250 (MQ=255)            aaaGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGt                                                                                                                                                                                                                                >  1:867841/1‑251 (MQ=255)                tGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGga                                                                                                                                                                                                                             <  1:545937/250‑1 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       <  1:306338/235‑1 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       >  1:444419/1‑235 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑Gcc                                                                                                                                                                                                                                   <  1:854655/238‑1 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAAt                                                                                                                                                                                                                      >  1:332220/1‑251 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAAt                                                                                                                                                                                                                      >  1:771208/1‑251 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAAt                                                                                                                                                                                                                      >  1:191412/1‑251 (MQ=255)                      cTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAAt                                                                                                                                                                                                                      >  1:23374/1‑251 (MQ=255)                         tGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGAAAGAAAATaaa                                                                                                                                                                                                                   <  1:646734/251‑1 (MQ=255)                             gCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTg                                                                                                                                                                                                               >  1:451977/1‑251 (MQ=255)                              cTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAg                                                                                                                                                                                                                 <  1:649625/248‑1 (MQ=255)                                     ccGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCCTTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCTTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGAt                                                                                                                                                                                                       >  1:865681/1‑251 (MQ=255)                                     ccGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGAt                                                                                                                                                                                                       <  1:170884/251‑1 (MQ=255)                                           cGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCg                                                                                                                                                                                                 >  1:863178/1‑251 (MQ=255)                                             cTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGt                                                                                                                                                                                               >  1:488022/1‑251 (MQ=255)                                                 tGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑aaa                                                                                                                                                                                          >  1:724205/1‑251 (MQ=255)                                                 tGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑aaa                                                                                                                                                                                          >  1:679239/1‑251 (MQ=255)                                                  ggATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑aaaa                                                                                                                                                                                         >  1:18367/1‑251 (MQ=255)                                                  ggATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGAGCGGTGGATTTTCCGGTT‑aaaa                                                                                                                                                                                         >  1:341466/1‑251 (MQ=255)                                                   gATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       >  1:80763/1‑206 (MQ=255)                                                     tttATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATg                                                                                                                                                                                      >  1:628614/1‑251 (MQ=255)                                                        aTTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAg                                                                                                                                                                                  <  1:533952/251‑1 (MQ=255)                                                           ggCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       <  1:535073/198‑1 (MQ=255)                                                            gCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGAt                                                                                                                                                                                                                                         <  1:573868/195‑1 (MQ=255)                                                                       cgctgcgctgGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAAtt                                                                                                                                                                   <  1:894990/250‑1 (MQ=255)                                                                           gcgctgGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGAt                                                                                                                                                                                                                                         >  1:204424/1‑180 (MQ=255)                                                                                gggTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAc                                                                                                                                                                                                                                       <  1:273895/177‑1 (MQ=255)                                                                                gggTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTcc                                                                                                                                                                                                  >  1:235660/1‑213 (MQ=255)                                                                                gggTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTa                                                                                                                                                         >  1:890228/1‑250 (MQ=255)                                                                                gggTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTa                                                                                                                                                         >  1:617461/1‑250 (MQ=255)                                                                                  gTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAt                                                                                                                                                      >  1:850960/1‑251 (MQ=255)                                                                                  gTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGACAC‑GACGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ACTTACAt                                                                                                                                                      >  1:178989/1‑251 (MQ=255)                                                                                   tATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAtt                                                                                                                                                     >  1:842188/1‑251 (MQ=255)                                                                                    aTTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAttt                                                                                                                                                    <  1:898799/251‑1 (MQ=255)                                                                                          aCCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑Ct                                                                                                                                             <  1:535490/250‑1 (MQ=255)                                                                                              cACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTc                                                                                                                                         <  1:316555/250‑1 (MQ=255)                                                                                              cACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTc                                                                                                                                         <  1:63986/250‑1 (MQ=255)                                                                                              cACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTc                                                                                                                                         >  1:758026/1‑250 (MQ=255)                                                                                                cTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGt                                                                                                                                                                                               >  1:502727/1‑200 (MQ=255)                                                                                                  tttACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAgg                                                                                                                                   <  1:780457/251‑1 (MQ=255)                                                                                                      cAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGaaaa                                                                                                                               >  1:356805/1‑251 (MQ=255)                                                                                                        aTTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGaaaaa                                                                                                                              >  1:300931/1‑250 (MQ=255)                                                                                                        aTTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑c                                                                                                                            <  1:795604/251‑1 (MQ=255)                                                                                                                           tCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAt                                                                                                                                                      <  1:332740/210‑1 (MQ=255)                                                                                                                               ttttCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCt                                                                                                      >  1:461499/1‑250 (MQ=255)                                                                                                                                          gtgtTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTc                                                                                                                                         >  1:503388/1‑206 (MQ=255)                                                                                                                                          gtgtTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGc                                                                                           >  1:99119/1‑250 (MQ=255)                                                                                                                                            gttgttTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCGCCGT‑GCAAATTAACG‑ATTTACATTTTG‑c‑c                                                                                                                                              >  1:427305/1‑199 (MQ=255)                                                                                                                                            gttgttTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑c‑c                                                                                                                                              >  1:485737/1‑199 (MQ=255)                                                                                                                                               gttTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCAc                                                                                                                                                                              >  1:423839/1‑168 (MQ=255)                                                                                                                                                tttATTTTACTGGCTTCAGATTTTTTCATTCCATGGCGCTTG‑TTGGTTTCTTTCTCCGGTGGGGGGAGAAAGGGCAAGGC‑GGTTGGGAAAAAAATCTTTACGCTGTTGAATAC‑GCGGGTGGAA‑AAAATAAAGGGGGGGTGGATTTCCCGGTT‑AAAAATA‑AAGTCACCGT‑GAAAATAAACG‑TTTTCCTTTTTG‑C‑CTTTTCA‑G‑GGAAAAACCCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAg                                                                                   <  1:205256/251‑1 (MQ=255)                                                                                                                                                 ttATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑Gcc                                                                                                                                                                                                                                   >  1:712810/1‑115 (MQ=255)                                                                                                                                                   aTTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGtt                                                                                 >  1:865289/1‑251 (MQ=255)                                                                                                                                                    ttttACTGGCTTCCGAATTTTTCTTCCCATGGCTCTTTCTTGG‑TTCTTTCTGCCAGGTGGCGATGAACGGCAAAGCGGCTGCGG‑AAAAAATCTTAACGCGGTTGAATAG‑GCCGGTGGAAAAAAATAAAAGTGCGGT‑GATTTTTCGGAT‑AAAAAAGAAAGCCCCCGT‑CCAAATAAACT‑ATTTACATTTTG‑C‑CTATCCC‑AAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTa                                                                                <  1:379935/251‑1 (MQ=255)                                                                                                                                                    ttttACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAt                                                                                                                                                      >  1:682526/1‑185 (MQ=255)                                                                                                                                                      ttACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTaa                                                                               >  1:499263/1‑250 (MQ=255)                                                                                                                                                             cTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCAt                                                                                                >  1:731589/1‑226 (MQ=255)                                                                                                                                                                        ttCATCCCATTGCGTTTGTTTGT‑TTCCTTCTTCCATGTGGCGATGAACGGAAAAGCTGCTTAGA‑AAAAATTCTTTACGTTGTTGGA‑ACGGCGGGTGAAAAAAAATAAAAGTGCGGTGGTTTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑TTTTAC‑TTTTGAC‑CTATCCC‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAg                                                            <  1:834088/251‑1 (MQ=255)                                                                                                                                                                                        tgcttgG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCg                                            >  1:624516/1‑251 (MQ=255)                                                                                                                                                                                         gcttgG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAgg                                                                                                                                   >  1:573758/1‑164 (MQ=255)                                                                                                                                                                                         gcttgG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGa                                           >  1:283042/1‑251 (MQ=255)                                                                                                                                                                                              g‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                            >  1:894449/1‑213 (MQ=255)                                                                                                                                                                                                 tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                            >  1:503100/1‑211 (MQ=255)                                                                                                                                                                                                 tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                    >  1:781522/1‑251 (MQ=255)                                                                                                                                                                                                 tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                    >  1:7662/1‑251 (MQ=255)                                                                                                                                                                                                 tCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTACTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGt                                    >  1:588155/1‑251 (MQ=255)                                                                                                                                                                                                  ctttctccCATGGGGCGATGAGCGGAAAAGCTGCTTGGG‑AAAAAATTTTTCCGTTGTTGGATAG‑GCGGGTGGAAAAAAAAAAAAGGGGGGTGGTTTTTCCGTTT‑AAAAAGG‑AATTCACCGT‑AAAAATTACCG‑TTTTACTTTTTG‑C‑CTATCCC‑GTGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTa                                   <  1:619018/249‑1 (MQ=255)                                                                                                                                                                                                  ccTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACAt                                                                                                                                                      <  1:373481/140‑1 (MQ=255)                                                                                                                                                                                                    ttctcccgtggggCGATGACCGGCAAAGCTGTTTCGA‑AAAAAATCTTTACGCTTTTGGATAC‑GCCGGTGGAAAAAAAAAAAAGTGCGGTGTATTTTCCGGTA‑AAAAATG‑AAGCCCCCGT‑GAAAATTACCG‑‑TTTTCCTTTTGCC‑CTATCCC‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATc                                 <  1:508853/240‑1 (MQ=255)                                                                                                                                                                                                     tcttcCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTAt                                  >  1:897044/1‑249 (MQ=255)                                                                                                                                                                                                       ttcCATGTGGCGATGAACGGCAAAGCGGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAAAAAAAGTGCGGTGGATTTTCCGGTA‑AAAAAGG‑AAGTCCCCGT‑GAAAATTAACT‑TTTTACATTTTG‑C‑CTATTCC‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCttt                              <  1:228891/251‑1 (MQ=255)                                                                                                                                                                                                        tcCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTa                             >  1:602651/1‑251 (MQ=255)                                                                                                                                                                                                         ccttgtgGCGATGAACGGAAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAAAAAAAGTGGGGTGTATTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GCAAATAAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGt                                                                           <  1:224303/201‑1 (MQ=255)                                                                                                                                                                                                               ggCGATGACCGGCAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAAAAAATAAAAGTGCGGTGGATTTTCCGGTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑ATTTACATTTTG‑C‑TTATCCC‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATg                       <  1:52825/250‑1 (MQ=255)                                                                                                                                                                                                                gCGATGAACGGCAAAGTTGCTTGGA‑AAAAAATCTTTACGTTGTGGGATAC‑GCGGGTGGAAAAAAAAAAAAGGGCGGTGATTTTTCCGGTA‑AAAAATG‑AAGTCACCGT‑GAAAATTAACG‑TTTTACTTTTTC‑C‑CTATCCC‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgg                      <  1:666532/250‑1 (MQ=255)                                                                                                                                                                                                                  gATGAACGACAAAGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGGAAGAAAAAAAAAGGGGGGTGG‑TTTTCCCGTTAAAAAATG‑AAGTCGCCGT‑GCAAATTAACG‑ATTTACTTTTTG‑C‑TTATTCC‑GGGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgggg                    <  1:88408/250‑1 (MQ=255)                                                                                                                                                                                                                  cgatgaaggaaaaagcgtctgcgaaaaaaaTCTTTACGCTGTTGGAGAC‑GCCGGGGAAAAAAAAAAAAAGTGGGGTGGATTTTCCGTTA‑AAAAATG‑AATTCCCCTG‑GCAAATT‑ACGAATTTCAATTTTG‑C‑CT‑TTCCAAAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATgggg                    <  1:760872/227‑1 (MQ=255)                                                                                                                                                                                                                        cGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt              >  1:161164/1‑250 (MQ=255)                                                                                                                                                                                                                        cGGCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt              >  1:874426/1‑250 (MQ=255)                                                                                                                                                                                                                        acggcaagtttacttagaaaaaaaTCTTCGCGCTGTGGGATAC‑GCGGGTGGAAAAAAATAAGTGTGTGAGAGATTTTCCATTA‑AAAAATG‑AGGCCA‑CGTAGCAAATTAACT‑AATAAC‑TTTTG‑CACTATACA‑GAGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTAt              <  1:893479/233‑1 (MQ=255)                                                                                                                                                                                                                          gCAAAGCTGCTTGGA‑AAAAAATCTTTACGCTGTTGGAGAC‑GCGGGTGAAAAAAAATAAAAGGGCGGTGGATTTTCGGGTA‑AAAAAGG‑AAGTCACCGT‑GCAAATTAACT‑ATTTACATTTTC‑C‑CTATTCG‑GTGGAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCa            <  1:575213/250‑1 (MQ=255)                                                                                                                                                                                                                          gCAAAGCTGCTTCGG‑AAAAAATCTTTACGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACgg                                                                            >  1:460582/1‑186 (MQ=255)                                                                                                                                                                                                                            aaaGCTGCTTCGA‑AAAAAATCTTTACGCTGTTGGATAC‑GCGGGTGAAAAAAAAAAAAAGTGGGGTGTATTTTCCGTTA‑AAAAATG‑AAGCCCCCGT‑ACAAATTAACG‑ATTTACATTTTC‑C‑CTATCCC‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGgc         <  1:794951/251‑1 (MQ=255)                                                                                                                                                                                                                                 tgctTCGG‑AAAAATTCTTTGCGTTGTTGGATCC‑GCCGGTGAAAGAAAAAAAAAGGGGGGTGGTTTTTCCGTTA‑AAAAATG‑AAGTCCCCGT‑GAAAATTAACG‑TTTTCCTTTTTG‑C‑CTATCCC‑GAGAAAAAC‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGcaac    <  1:238786/251‑1 (MQ=255)                                                                                                                                                                                                                                   tttCGA‑AAAAAATCTTTACGCTTTGGGATCC‑GCGGGTGGAAAAAAATAAAAGTGGGGTGGATTTTCCGGTA‑AAAAATA‑AAGCCCCCGT‑GAAAATTAACG‑ATTTACTTTTTC‑C‑CTATCCC‑GAGAAAAAA‑CCCGCCATTAACGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGcaacaa  <  1:344681/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CTATTACCATGAAAGTGCTCACTATGCAGCTGAACTCCGTTTCGCTAATGGATTTATTGGCTTACGGCGGCGCTGCGCTGGGTATTTTGACCGCACTTTTACAATTTCAGGACGGCAATTTATCCATTTTCGGCGTGGTGTTGTTTATTTTACTGGCTTCCGAATTTTTCATTCCATTGCGCTTGCTTGG‑TTCCTTCTTCCATGTGGCGATGAACGGCAAAGCTGCTTCGG‑AAAAAATCTTTATGCTGTTGGATAC‑GCCGGTGGAAGAAAATAAAAGTGCGGTGGATTTTCCGGTT‑AAAAATG‑AAGTCACCGT‑GCAAATTAACG‑ATTTACATTTTG‑C‑CTATTCC‑GAGGAAAAA‑CCCGCCATTAAAGGCTTAACCCTCAGCATCCAGCCGAAACAGTTAACGGTTTTCGTGGGCAAAAGCGGTTGTGGCAAATCGACCTTGGTATCTTTACTAATGGGGTTCTATCAGGCGCAACAA  >  2/133077‑133552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑