Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	131,108	T→C	G71G (GGT→GGC)	unknown →	Anaerobic C4‑dicarboxylate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	131,108	0	T	C	100.0%	103.1 / NA	44	G71G (GGT→GGC)	unknown	Anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/32);  total (12/32)

TTTTTATTCTAAGGAGGCAGTATGTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGTGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTATTGTTGCAAGCCCGATTGCGGCGG  >  2/130875‑131352                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                            tttttattCTAAGGAGGCAGTATGTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCCGCGGCAATGCAGGCTGCCGGCggggttggttttattgt                                                                                                                                                                                                                                            >  1:753784/1‑237 (MQ=255)                ggCAGTATGTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGGTTGATTTAATGAT‑C‑aaaaattcccccaa                                                                                                                                                                                                                           >  1:711650/1‑241 (MQ=255)                  cAGTATGTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGGTTGATTACATGATCC‑AAAAtttccccaaaa                                                                                                                                                                                                                          >  1:374781/1‑242 (MQ=255)                        gTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCCGCGGGGTTGAGTTCATGAT‑T‑AAAAATGTCACTAATAtttttt                                                                                                                                                                                                                   >  1:44126/1‑249 (MQ=255)                               aTGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGGTTGATTTCATTAT‑C‑CAAATTTCCCCTAAAAttttttgtcgtta                                                                                                                                                                                                            >  1:156838/1‑242 (MQ=255)                                  tttttATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑c                                                                                                                                                                                                       <  1:648825/250‑1 (MQ=255)                                       aTTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAAt                                                                                                                                                                                                 <  1:627669/251‑1 (MQ=255)                                                cGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACtt                                                                                                                                                                                        <  1:698622/251‑1 (MQ=255)                                                    aTTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGGTTACATGAT‑C‑AAAATTg                                                                                                                                                                                                                                  <  1:820753/208‑1 (MQ=255)                                                       gtgtTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATgg                                                                                                                                                                                  <  1:431759/250‑1 (MQ=255)                                                                    ttttAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCCGCGGCAATGCCGGCTGCCGGCGGGGTAGAATACATGAT‑C‑AAAAATGCCCCTAATATTTTTTGTC‑GTAAAT‑CC‑AAATACATTACTTTTTTTGCAccctcgggaaat                                                                                                                                                                    >  1:464212/1‑241 (MQ=255)                                                                    ttttAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATgg                                                                                                                                                                                  <  1:18258/237‑1 (MQ=255)                                                                             gTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGGTTGGTTACATGGT‑T‑AAAAATGCCCCCAATATTTTTCCGC‑GT‑AATCCCGAAA‑AAATTACTTTTTTGGCAcccgccggtacttgggccttt                                                                                                                                                           >  1:439575/1‑232 (MQ=255)                                                                                          cGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAg                                                                                                                                              <  1:494170/251‑1 (MQ=255)                                                                                             aaTCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGAc                                                                                                                                                               <  1:498281/231‑1 (MQ=255)                                                                                              aTCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AAc                                                                                                                                         <  1:42644/251‑1 (MQ=255)                                                                                              aTCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AAc                                                                                                                                         <  1:696608/251‑1 (MQ=255)                                                                                                  gTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAAc                                                                                                                                                                     <  1:812442/220‑1 (MQ=255)                                                                                                  gTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AAc                                                                                                                                         <  1:446020/247‑1 (MQ=255)                                                                                                          gtATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTg                                                                                                                                                     <  1:569241/228‑1 (MQ=255)                                                                                                            aTTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTg                                                                                                                                                     <  1:104292/226‑1 (MQ=255)                                                                                                                 gtggCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTc                                                                                                                       <  1:799607/250‑1 (MQ=255)                                                                                                                    gCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCCATGCAGGCTGCCGGCGGGGTTGATTTAATTAT‑C‑AAAAATGGCACCAAAATTTTTCGTCGGT‑AAT‑CGGAAA‑AAATTACTTTTTTTGCACCCGCGGGAACTTTGAAATTTTCGCTGTTTGCAGGAAACCGG‑CCCGTGGCTTTCTcc                                                                                                                      >  1:809641/1‑248 (MQ=255)                                                                                                                                         ccTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTAc                                                                                                                         <  1:633357/224‑1 (MQ=255)                                                                                                                                                    ggTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGc                                                                                                                                                <  1:516307/191‑1 (MQ=255)                                                                                                                                                     gTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTc                                                                                                                       <  1:878435/214‑1 (MQ=255)                                                                                                                                                            aCCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTc                                                                          <  1:880987/251‑1 (MQ=255)                                                                                                                                                                          cAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCg                                                            <  1:637700/251‑1 (MQ=255)                                                                                                                                                                             ttGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATgg                                                                               <  1:265857/229‑1 (MQ=255)                                                                                                                                                                                atgtgatgtTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATcc                                                      <  1:413116/251‑1 (MQ=255)                                                                                                                                                                                atgtgatgtTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATcc                                                      <  1:269593/251‑1 (MQ=255)                                                                                                                                                                                atgtgatgtTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATc                                                       <  1:81320/250‑1 (MQ=255)                                                                                                                                                                                atgtgatgtTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGGTAGATTACATGAT‑C‑AAAAATGCCACTAATATTTTTTGTC‑GT‑AAT‑CCCaaaa                                                                                                                                                                                                 >  1:87549/1‑113 (MQ=255)                                                                                                                                                                                       gtTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATAc                                                                                                                                                                                               <  1:711930/109‑1 (MQ=255)                                                                                                                                                                                          tATGATTATGGCGGTAGTTGCAGCGGCCGCGGCAATGCAGGCTGCCGGCGGGGTTGATTACATGAT‑CAAAAATTG‑CACTAATATTTTTCGTC‑GT‑AAT‑CCGGAATACATTACTTTTTTTGCACCCGCGGGAAATTTGACATTTTCGCTTTTTGCAGG‑GAACGGTCACGTGGCTTTATCCGTTTTTACGGGTT‑TTGCTGAAGTGAGGCGTCAAAAAGGGATTTGCCCCGGGCGGCCGTTAttccttgcgggtt                                            >  1:3732/1‑239 (MQ=255)                                                                                                                                                                                               ttATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTc                                                                          <  1:93008/216‑1 (MQ=255)                                                                                                                                                                                                         aGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATTGCATCACaaattt                             >  1:582325/1‑247 (MQ=255)                                                                                                                                                                                                         aGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGGTATCGCATCACAAtttt                             >  1:438019/1‑250 (MQ=255)                                                                                                                                                                                                           ttGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCt                           <  1:704529/251‑1 (MQ=255)                                                                                                                                                                                                            tGCAGCGGCAGCGGCAATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTa                          <  1:191628/251‑1 (MQ=255)                                                                                                                                                                                                                         gcaATGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTTCCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTATTGTTGCAAGcc              <  1:644153/250‑1 (MQ=255)                                                                                                                                                                                                                            aTGCAGGCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATcc                                                      <  1:248892/207‑1 (MQ=255)                                                                                                                                                                                                                                  gCTGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTATTGTTGCAAGCCCGATTgc       <  1:692469/248‑1 (MQ=255)                                                                                                                                                                                                                                    tGCCGGCGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTATTGTTGCAAGCCCGATTgcggcgg  <  1:73575/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                            TTTTTATTCTAAGGAGGCAGTATGTCTGCTATGTTTTTATTTCAGTTCGCCATTGTGTTGCTCTGCATTTTAATCGGTGCGCGTGTTGGCGGAATCGGTTTAGGTGTATTTGGTGGCTTGGGGCTTACAATTTTGTCCTTTGGGTTCGGTTTAAAACCTGCCGGCTTGCCAATTGATGTGATGTTTATGATTATGGCGGTAGTTGCAGCGGCAGCGGCAATGCAGGCTGCCGGTGGGTTAGATTACATGAT‑C‑AAAATTGCCACTAATATTTTACGTC‑GT‑AAT‑CCGAAATACATTACTTTTATGGCACCTGCGGTAACTTGGACATTTACGCTGTTAGCAGG‑AACCGGTCACGTGGCTTACTCCG‑TATTACCGGTTATCGCTGAAGTGAGCCGTCAAAATGGTATTCGCCCGGAGCGTCCGTTATCCATGGCGGTTATCGCATCACAATTTGCTATTGTTGCAAGCCCGATTGCGGCGG  >  2/130875‑131352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑