Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	125,093	T→C	intergenic (‑395/+48)	unknown ← / ← unknown	tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG/Flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	125,093	0	T	C	100.0%	142.4 / NA	51	intergenic (‑395/+48)	unknown/unknown	tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG/Flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/13);  total (38/13)

GTTTATTATTACTATAATTAAGATCGACGAAAACCTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTATCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAATTGCCCAAGCCGATGACGGCAAATCGTAA  >  2/124878‑125335                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gTTTATTATTACTATAATTAAGATCGACGAAAACCTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGattttattt                                                                                                                                                                                                                 >  1:245006/1‑251 (MQ=255)              ataATTAAGATCGACGAAAACCTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGAt                                                                                                                                                                                                    >  1:848927/1‑251 (MQ=255)                aaTTAAGATCGACGAAAACCTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTa                                                                                                                                                                                                  >  1:713715/1‑251 (MQ=255)                                  ccTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGtt                                                                                                                                                                                >  1:719547/1‑251 (MQ=255)                                     tGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCcac                                                                                                                                                                                                                                                <  1:832033/184‑1 (MQ=255)                                          aaGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGa                                                                                                                                                                                              <  1:621070/229‑1 (MQ=255)                                                         ttgtAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGaa                                                                                                                                                                                                                                  >  1:213919/1‑178 (MQ=255)                                                              aaTGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTcc                                                                                                                                                    <  1:642827/251‑1 (MQ=255)                                                              aaTGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTc                                                                                                                                                     >  1:120683/1‑250 (MQ=255)                                                                 gAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGa                                                                                                                                                 >  1:120847/1‑251 (MQ=255)                                                                             tCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGtt                                                                                                                                     >  1:227800/1‑251 (MQ=255)                                                                                    aCAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGt                                                                                                                                      >  1:645125/1‑243 (MQ=255)                                                                                      aaGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCa                                                                                                                            >  1:321081/1‑251 (MQ=255)                                                                                          tgttAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCATGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATAc                                                                                                                        <  1:182215/251‑1 (MQ=255)                                                                                           gttAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACg                                                                                                                       >  1:879117/1‑251 (MQ=255)                                                                                                gATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGattttattt                                                                                                                                                                                                                 >  1:273699/1‑156 (MQ=255)                                                                                                     tACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGc                                                                                                                      >  1:719634/1‑242 (MQ=255)                                                                                                     tACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCg                                                                                                             >  1:678869/1‑251 (MQ=255)                                                                                                      aCCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGa                                                                                                            >  1:545028/1‑251 (MQ=255)                                                                                                                 tcAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGAcc                                                                                                 <  1:584824/251‑1 (MQ=255)                                                                                                                            gtgtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGc                                                                                                                                                              >  1:240116/1‑179 (MQ=255)                                                                                                                            gtgtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCaa                                                                                                                    >  1:324277/1‑221 (MQ=255)                                                                                                                            gtgtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTg                                                                                       >  1:591623/1‑250 (MQ=255)                                                                                                                            gtgtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTg                                                                                       >  1:593121/1‑250 (MQ=255)                                                                                                                              gtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAag                                                                                                                                                                                                                                             <  1:661073/98‑1 (MQ=255)                                                                                                                              gtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGAt                                                                                                                                                >  1:328305/1‑191 (MQ=255)                                                                                                                              gtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGcc                                                                                     <  1:201429/250‑1 (MQ=255)                                                                                                                              gtATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGc                                                                                      >  1:482230/1‑249 (MQ=255)                                                                                                                                   aCTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAg                                                                                                                                                                                               >  1:315912/1‑139 (MQ=255)                                                                                                                                   aCTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAg                                                                               >  1:341610/1‑251 (MQ=255)                                                                                                                                     tctGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGt                                                                             <  1:283925/251‑1 (MQ=255)                                                                                                                                      ctGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCGCATTGTTCCTGATCGGTTTCCGtt                                                                                                                                     >  1:424444/1‑194 (MQ=255)                                                                                                                                      ctGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGAc                                                                                                                                >  1:691844/1‑199 (MQ=255)                                                                                                                                                tCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCg                                                                  <  1:296087/251‑1 (MQ=255)                                                                                                                                                      gTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCa                                                            >  1:863237/1‑251 (MQ=255)                                                                                                                                                                     aaaTTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAgg                                             >  1:301595/1‑251 (MQ=255)                                                                                                                                                                       aTTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGa                                                                                                                                                                                              >  1:103191/1‑104 (MQ=255)                                                                                                                                                                       aTTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTa                                           >  1:404345/1‑251 (MQ=255)                                                                                                                                                                         ttATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGAc                                                                                                  >  1:833012/1‑194 (MQ=255)                                                                                                                                                                             tCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAg                                     <  1:70742/251‑1 (MQ=255)                                                                                                                                                                               gtgtTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGt                                    >  1:495073/1‑250 (MQ=255)                                                                                                                                                                                   tataAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAg                                                                                                                                  >  1:792444/1‑152 (MQ=255)                                                                                                                                                                                    ataAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAATAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTc                                                                       <  1:706677/210‑1 (MQ=255)                                                                                                                                                                                    ataAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGAc                                                                                                                                >  1:95231/1‑153 (MQ=255)                                                                                                                                                                                    ataAAAACAGATTTTTCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAAt                              <  1:372784/251‑1 (MQ=255)                                                                                                                                                                                                 tttCACAATTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAATTGCCCAAGCCGAt                 >  1:214107/1‑251 (MQ=255)                                                                                                                                                                                                        aTTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATcc                                                             >  1:144054/1‑200 (MQ=255)                                                                                                                                                                                                        aTTTTATGTGAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAATTGCCCAAGCCGATGACGGCa          >  1:759835/1‑251 (MQ=255)                                                                                                                                                                                                                 gAAGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAg                                                                                                                                                                                               >  1:564747/1‑61 (MQ=255)                                                                                                                                                                                                                  aaGTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAATTGCCCAAGCCGATGACGGCAAATCGTaa  >  1:147442/1‑249 (MQ=255)                                                                                                                                                                                                                    gTTACCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTg                                                                                                                                                  <  1:766000/103‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GTTTATTATTACTATAATTAAGATCGACGAAAACCTTGGATAAGATCTTTTTTATGTTGTAAATGAAGGAGATGGATCATGCTACAAGTTGTTAAGATCTTACCGATCCTCCTCAAATTTCTTGTGTATAACTCTGTTATTTATCCACAGTTGGATCTTTTGTAAAATTTATTCGTGTTATAAAAACAGATTTTTCACAATTTTATGTGAAGTTATCCACAAGAGGTTTTAGAAAATGACTGATTTTATTTTGAACAAAAGGATTAAAGATGGTGCAGAAAGTTAGGCAGCCAGTCTTCCGCACATTGTTCCTGATCGGTTTCCGTTAAGACATCAATACGCAAAGCGTCGATAATTTTGACCGCACTTTTTGCCTGCAAGGTCTGTTCTACCGTATCCACCGCAAAACAGAAGGTATCATAGTCGGAATTGCCCAAGCCGATGACGGCAAATCGTAA  >  2/124878‑125335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑