Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	123,238	T→C	P487P (CCA→CCG)	unknown ←	tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	123,238	0	T	C	100.0%	259.5 / NA	94	P487P (CCA→CCG)	unknown	tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/40);  total (54/40)

TTTTCGTGGCGTTTTTGTTTTTCGATTTCTTCTTCTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGTGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAATATAAGCCTGATC  >  2/122999‑123483                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ttttCGTGGCGTTTTTGTTTTTCGATTTCTTCTTCTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAAcc                                                                                                                                                                                                                                             <  1:158292/250‑1 (MQ=255) ttttCGTGGCGTTTTTGTTTTTCGATATCTTCTTCTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAAcc                                                                                                                                                                                                                                             <  1:890465/250‑1 (MQ=255)         gCGTTTTTGTTTTTCGATTTCTTCTTCTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAAtgctg                                                                                                                                                                                                                                     >  1:892725/1‑250 (MQ=255)                           ttcttcttcTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCaa                                                                                                                                                                                                                  >  1:161733/1‑251 (MQ=255)                            tcttcttctTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAg                                                                                                                                                                                                                 <  1:49212/251‑1 (MQ=255)                               tcttcttGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTc                                                                                                                                                                                                              <  1:300028/251‑1 (MQ=255)                                   cttGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAata                                                                                                                                                                                                          >  1:175416/1‑251 (MQ=255)                                    ttGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAatat                                                                                                                                                                                                         <  1:183490/251‑1 (MQ=255)                                    ttGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAatat                                                                                                                                                                                                         <  1:895467/251‑1 (MQ=255)                                        tGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTc                                                                                                                                                                                                     <  1:16128/251‑1 (MQ=255)                                         gTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACg                                                                                                                                                                                                                             >  1:169256/1‑226 (MQ=255)                                         gTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAatat                                                                                                                                                                                                         <  1:584886/246‑1 (MQ=255)                                         gTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTc                                                                                                                                                                                                     >  1:393866/1‑250 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAatat                                                                                                                                                                                                         >  1:689419/1‑241 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATAtttt                                                                                                                                                                                                      <  1:529300/244‑1 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTcc                                                                                                                                                                                                    >  1:653144/1‑246 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCAtttt                                                                                                                                                                                               >  1:562744/1‑251 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCAtttt                                                                                                                                                                                               >  1:653330/1‑251 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCAtttt                                                                                                                                                                                               >  1:119481/1‑251 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCAtttt                                                                                                                                                                                               >  1:415113/1‑251 (MQ=255)                                              atatAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAAT‑AATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCAttttt                                                                                                                                                                                              >  1:691595/1‑251 (MQ=255)                                                 taACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAAtgc                                                                                                                                                                                                                                       >  1:216811/1‑208 (MQ=255)                                                    ccTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGAtt                                                                                                                                                                                         >  1:735173/1‑251 (MQ=255)                                                         atatTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAAc                                                                                                                                                                                    <  1:481006/251‑1 (MQ=255)                                                           atTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAAcg                                                                                                                                                                                   >  1:176435/1‑250 (MQ=255)                                                           atTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAAcg                                                                                                                                                                                   >  1:732886/1‑250 (MQ=255)                                                              tAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAAt                                                                                                                                                                                                                                         <  1:779665/193‑1 (MQ=255)                                                              tAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACgcgc                                                                                                                                                                                >  1:851910/1‑250 (MQ=255)                                                                           cACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTtcat                                                                                                                                                                  >  1:751978/1‑251 (MQ=255)                                                                            aCTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTtcatc                                                                                                                                                                 <  1:838515/251‑1 (MQ=255)                                                                             cTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTtcatca                                                                                                                                                                >  1:2501/1‑251 (MQ=255)                                                                                gTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAAtt                                                                                                                                                             >  1:516221/1‑251 (MQ=255)                                                                                gTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAAtt                                                                                                                                                             >  1:50656/1‑251 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAGATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTaa                                                                                                                                                     >  1:125512/1‑251 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGTGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAGCACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTtcatc                                                                                                                                                                 <  1:443811/239‑1 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAAtgctg                                                                                                                                                                                                                                     <  1:707041/171‑1 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAAtgc                                                                                                                                                                                                                                       >  1:68282/1‑169 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACg                                                                                                                                                                                                                             >  1:489256/1‑179 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTaa                                                                                                                                                     >  1:260185/1‑251 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTaa                                                                                                                                                     >  1:127769/1‑251 (MQ=255)                                                                                        gCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATACGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTaa                                                                                                                                                     >  1:442878/1‑251 (MQ=255)                                                                                              ttATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTg                                                                                                                                               <  1:476925/251‑1 (MQ=255)                                                                                                  cTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAatat                                                                                                                                                                                                         <  1:154460/189‑1 (MQ=255)                                                                                                  cTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCa                                                                                                                                           >  1:564052/1‑251 (MQ=255)                                                                                                     ccATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTc                                                                                                                                                  <  1:281530/241‑1 (MQ=255)                                                                                                               ttGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAAc                                                                                                                              <  1:683995/251‑1 (MQ=255)                                                                                                                    aaGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAata                                                                                                                         <  1:302835/251‑1 (MQ=255)                                                                                                                                aagTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCt                                                                                                             <  1:652207/251‑1 (MQ=255)                                                                                                                                        aTCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTaa                                                                                                     >  1:345103/1‑251 (MQ=255)                                                                                                                                            cATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTaacaac                                                                                                 <  1:52781/251‑1 (MQ=255)                                                                                                                                                    aTTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCGACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGata                                                                                         >  1:901940/1‑251 (MQ=255)                                                                                                                                                     tttCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGatat                                                                                        >  1:480427/1‑251 (MQ=255)                                                                                                                                                                  cAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTGAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAg                                                                           >  1:453990/1‑251 (MQ=255)                                                                                                                                                                       aaTCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAac                                                                      <  1:759432/251‑1 (MQ=255)                                                                                                                                                                       aaTCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAac                                                                      <  1:733963/251‑1 (MQ=255)                                                                                                                                                                         tCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAac                                                                      <  1:821028/249‑1 (MQ=255)                                                                                                                                                                             cACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGa                                                                >  1:625405/1‑251 (MQ=255)                                                                                                                                                                                   tGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGAGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGt                                                          <  1:8989/251‑1 (MQ=255)                                                                                                                                                                                        tCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCttt                                                     <  1:204890/251‑1 (MQ=255)                                                                                                                                                                                             cACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTaac                                                                                                    >  1:83537/1‑199 (MQ=255)                                                                                                                                                                                             cACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTa                                                 >  1:661802/1‑250 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAAcc                                                                                                                                                        >  1:274002/1‑145 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCg                                                                                            >  1:314309/1‑205 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACAcg                                                                   >  1:160026/1‑230 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACAcg                                                                   >  1:327963/1‑230 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:604693/1‑251 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:40009/1‑251 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:887923/1‑251 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:650929/1‑251 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:597991/1‑251 (MQ=255)                                                                                                                                                                                               ccAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGCGCAACCAAATGCTGCGTAAACGTTGGCGTTCACGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCa                                              >  1:231054/1‑251 (MQ=255)                                                                                                                                                                                                 aaTGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAg                                            <  1:188111/251‑1 (MQ=255)                                                                                                                                                                                                   tGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAggg                                          <  1:105620/251‑1 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCgg                                                                                                                     >  1:21263/1‑170 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTaacaac                                                                                                 >  1:232974/1‑190 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACggg                                                                              <  1:467653/209‑1 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAg                                                                           >  1:648915/1‑212 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTc                                                        <  1:238915/231‑1 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCaaa                                    >  1:713203/1‑251 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCaaa                                    >  1:608672/1‑251 (MQ=255)                                                                                                                                                                                                         aCCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCAGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACAcgc                                                                  >  1:880266/1‑221 (MQ=255)                                                                                                                                                                                                          cccAACACTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAg                                   <  1:107741/251‑1 (MQ=255)                                                                                                                                                                                                                 cTTTATTCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGt                                                          <  1:515776/221‑1 (MQ=255)                                                                                                                                                                                                                       tCGCTTCTTCCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAac                      <  1:733927/251‑1 (MQ=255)                                                                                                                                                                                                                            tcttcCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACAcc                 <  1:569260/251‑1 (MQ=255)                                                                                                                                                                                                                             cttcCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAg                                   <  1:44532/232‑1 (MQ=255)                                                                                                                                                                                                                             cttcCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCa                >  1:424152/1‑251 (MQ=255)                                                                                                                                                                                                                               tcCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCata              >  1:690035/1‑249 (MQ=255)                                                                                                                                                                                                                               tcCAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAat              <  1:502722/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAAATATTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAatat            >  1:220962/1‑251 (MQ=255)                                                                                                                                                                                                                                      atTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAATATAAGcc       <  1:701521/251‑1 (MQ=255)                                                                                                                                                                                                                                      atTCTGAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAATATAAGcc       <  1:878147/251‑1 (MQ=255)                                                                                                                                                                                                                                           gAACGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAacac                    <  1:883391/233‑1 (MQ=255)                                                                                                                                                                                                                                            aaCGCGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAATATAAGCCTGATc  <  1:547888/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      TTTTCGTGGCGTTTTTGTTTTTCGATTTCTTCTTCTTGATGTTCAATATAACCTTGATATTTAATAGCAATTTCCACTTGTTCCACTGCTTCTTTATCTTCCATTGCTGGTTGATAAGGCGTCAAAGAAGTTAAAATCTCATAGTTCATTTCCGGGCGACGCAATAAATCTTCACCGTTGGCTTCGCGCACCAATGGACTACCCAACACTTTATTCGCTTCTTCCAAATATTCTGAACGTGGGTGCAACCAAATGCTGCGTAAACGTTGGCGTTCAAGCTCAATATTTTCCATTTTTTGATTAAAACGCGCCCAACGCGCTTCATCAATTAAACCTAATTCGTGCGCAATCGGCGTTAAACGAATATCGGCGTTATCTTCACGTAACAACAAGCGATATTCCGCACGGGAAGTAAACACGCGATATGGTTCTTTAGTACCAAGGGTGCAAAGATCATCCACCAACACACCAATATAAGCCTGATC  >  2/122999‑123483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑