Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	117,581	A→C	Y69D (TAT→GAT)	unknown ←	ATP synthase alpha chain (EC 3.6.3.14)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	117,581	0	A	C	100.0%	228.1 / NA	80	Y69D (TAT→GAT)	unknown	ATP synthase alpha chain (EC 3.6.3.14)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/40);  total (40/40)

GGTTTGAACAGGTTGATCAACGGACTTACGTTCAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGATTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTATAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATATCTTTCTAAGTTTAATGCCATTGCGTAGCTATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTGCAACTCATTTGC  >  2/117342‑117818                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              ggTTTGAACAGGTTGATCAACGGACTTACGTTCAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGt                                                                                                                                                                                                                                    >  1:563677/1‑251 (MQ=255)           ggTTGATCAACGGACTTACGTTCAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCAt                                                                                                                                                                                                                          >  1:82368/1‑251 (MQ=255)                  cAACGGACTTACGTTCAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAg                                                                                                                                                                                                                   <  1:640763/251‑1 (MQ=255)                                 cAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGttt                                                                                                                                                                                                                                  >  1:801219/1‑221 (MQ=255)                                 cAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAAt                                                                                                                                                                                                    >  1:284340/1‑251 (MQ=255)                                   aTTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATg                                                                                                                                                                                                   >  1:124683/1‑250 (MQ=255)                                        acCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAatca                                                                                                                                                                                             <  1:279628/251‑1 (MQ=255)                                         cccGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAatcat                                                                                                                                                                                            >  1:21370/1‑251 (MQ=255)                                            ggTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAACACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCAtct                                                                                                                                                                                          >  1:508778/1‑250 (MQ=255)                                                    gACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGcga                                                                                                                                                                                                                <  1:881304/220‑3 (MQ=255)                                                     aCTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCa                                                                                                                                                                                 <  1:558239/250‑1 (MQ=255)                                                         cAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCAcc                                                                                                                                                                                      <  1:36796/241‑1 (MQ=255)                                                               gagaAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATcacttcactt                                                                                                                                                                      >  1:548661/1‑251 (MQ=255)                                                                   aaaaCCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAGTGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  <  1:186957/251‑1 (MQ=255)                                                                   aaaaCCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  <  1:115172/251‑1 (MQ=255)                                                                   aaaaCCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  <  1:660537/251‑1 (MQ=255)                                                                   aaaaCCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  <  1:679307/251‑1 (MQ=255)                                                                     aaCCGTCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCg                                                                                                                                                                <  1:697828/251‑1 (MQ=255)                                                                          tCATTTTCAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGaa                                                                                                                                                            <  1:131186/250‑1 (MQ=255)                                                                                 cAATTTCACCTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAATCCGCGACCGACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATg                                                                                                                                                    <  1:318348/251‑1 (MQ=255)                                                                                         ccTTTGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCAt                                                                                                                                             >  1:893770/1‑250 (MQ=255)                                                                                           tttGCCGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGc                                                                                                                                          <  1:128208/251‑1 (MQ=255)                                                                                               ccGTCAATCGGTTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGctga                                                                                                                                       >  1:643759/1‑250 (MQ=255)                                                                                                            gACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  >  1:565171/1‑210 (MQ=255)                                                                                                                  gggTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATAcc                                                                                                                                               <  1:851136/223‑1 (MQ=255)                                                                                                                  gggTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTa                                                                                                                   >  1:706666/1‑251 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGcga                                                                                                                                                                                                                >  1:6265/1‑154 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCGCTTCACTTAAACCGTGAATACGAATGATAcc                                                                                                                                               <  1:197009/221‑1 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTAtt                                                                                                                 >  1:385661/1‑251 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTAtt                                                                                                                 >  1:420138/1‑251 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTAt                                                                                                                  <  1:26850/250‑1 (MQ=255)                                                                                                                    gTATTTACCACACGACCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTAt                                                                                                                  <  1:126097/250‑1 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATAc                                                                                                                                                         <  1:281370/197‑1 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATAc                                                                                                                                                <  1:710767/206‑1 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGa                                                                                                                              <  1:224628/224‑1 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTAcgc                                                                                                             <  1:64364/241‑1 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCttt                                                                                                   >  1:553142/1‑251 (MQ=255)                                                                                                                                  aCCTAATAAACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCttt                                                                                                   >  1:727684/1‑251 (MQ=255)                                                                                                                                      aataaACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTAcgc                                                                                                             >  1:647551/1‑237 (MQ=255)                                                                                                                                      aataaACCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTa                                                                                                >  1:572124/1‑250 (MQ=255)                                                                                                                                          aaCCGCGACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTa                                                                                           >  1:819503/1‑251 (MQ=255)                                                                                                                                             cgcgACCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAAc                                                                                        <  1:350547/251‑1 (MQ=255)                                                                                                                                             cgcgACCTACAGGCACTTCAAGAATACCGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCGTCTCACCTTGCATCACTTCACTTAATCCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTGCATTAAAc                                                                                        <  1:612693/251‑1 (MQ=255)                                                                                                                                                 aCCTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCAtcactt                                                                                                                                                                           >  1:530770/1‑164 (MQ=255)                                                                                                                                                  ccTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGc                                                                                   <  1:848107/251‑1 (MQ=255)                                                                                                                                                  ccTACAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGc                                                                                   <  1:577862/251‑1 (MQ=255)                                                                                                                                                   ctacaGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATg                                                                                                                                                    >  1:601635/5‑185 (MQ=255)                                                                                                                                                                   aaTACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTttatt                                                                   <  1:81781/250‑1 (MQ=255)                                                                                                                                                                             gTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAAc                                                                                                                                                                  >  1:810369/1‑145 (MQ=255)                                                                                                                                                                             gTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAAc                                                                                        <  1:570472/219‑1 (MQ=255)                                                                                                                                                                             gTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGc                                                                                   >  1:257704/1‑224 (MQ=255)                                                                                                                                                                             gTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAAt                                                        >  1:621519/1‑251 (MQ=255)                                                                                                                                                                               aCATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAAttt                                                      >  1:159207/1‑251 (MQ=255)                                                                                                                                                                                cATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCa                                                                                                                                                                                 <  1:31970/127‑1 (MQ=255)                                                                                                                                                                                  ttGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAg                                                   >  1:60484/1‑251 (MQ=255)                                                                                                                                                                                      aCTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTg                                                <  1:250362/250‑1 (MQ=255)                                                                                                                                                                                         tCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAAtt                                            >  1:505925/1‑251 (MQ=255)                                                                                                                                                                                             tGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGt                                        <  1:469501/251‑1 (MQ=255)                                                                                                                                                                                               ccTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTAt                                                                                                                  <  1:92198/175‑1 (MQ=255)                                                                                                                                                                                                 ttCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCg                                                                             >  1:223472/1‑210 (MQ=255)                                                                                                                                                                                                 ttCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCa                                    >  1:261736/1‑251 (MQ=255)                                                                                                                                                                                                   cTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCAt                                   >  1:781592/1‑250 (MQ=255)                                                                                                                                                                                                          aTCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAAt                           <  1:231731/251‑1 (MQ=255)                                                                                                                                                                                                           tCGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATc                          <  1:654620/251‑1 (MQ=255)                                                                                                                                                                                                            cGGCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATcc                         <  1:723370/251‑1 (MQ=255)                                                                                                                                                                                                              gCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAAc                                                                                        >  1:242497/1‑186 (MQ=255)                                                                                                                                                                                                              gCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATcc                         >  1:505768/1‑249 (MQ=255)                                                                                                                                                                                                              gCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCct                        >  1:710251/1‑250 (MQ=255)                                                                                                                                                                                                              gCATAAGGCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCct                        >  1:244274/1‑250 (MQ=255)                                                                                                                                                                                                                     gCCCCATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAAttt                                                      >  1:54197/1‑213 (MQ=255)                                                                                                                                                                                                                      ccccATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAAt                <  1:817820/250‑1 (MQ=255)                                                                                                                                                                                                                       cccATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTg              >  1:306270/1‑251 (MQ=255)                                                                                                                                                                                                                       cccATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTg              <  1:532306/251‑1 (MQ=255)                                                                                                                                                                                                                         cATTACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATa                                                                                                                                                          <  1:825577/109‑1 (MQ=255)                                                                                                                                                                                                                           ttACCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATc                          >  1:565329/1‑235 (MQ=255)                                                                                                                                                                                                                             aCCACTGCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAAt                <  1:399419/243‑1 (MQ=255)                                                                                                                                                                                                                                   gCACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTGc             >  1:896149/1‑240 (MQ=255)                                                                                                                                                                                                                                    cACCAACAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTGCAACTCATTTGc  >  1:169558/1‑250 (MQ=255)                                                                                                                                                                                                                                        aaCAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGc                                                                                   <  1:31251/165‑1 (MQ=255)                                                                                                                                                                                                                                          cAGAATCTCTTTCTAAGTTTAATGCCATTGCGTAGCGATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAAt                <  1:185643/230‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              GGTTTGAACAGGTTGATCAACGGACTTACGTTCAATTACACCCGGTGCAATGACTTCAATTGGAGAAAAACCGTCATTTTCAATTTCACCTTTGCCGTCAATCGATTGACCAAGGGTATTTACCACACGACCTAATAAACCGCGACCTATAGGCACTTCAAGAATACGGCCGGTACATTGGACTTCCATGCCTTCTGCAAGATCGGCATAAGGCCCCATTACCACTGCACCAACAGAATATCTTTCTAAGTTTAATGCCATTGCGTAGCTATTTCCCGGTAATGCAATCATCTCACCTTGCATCACTTCACTTAAACCGTGAATACGAATGATACCATCGCTGACTGAAACGATTGTTCCCGTATTACGCGCTTCGCTTTCTACATTAAACTGGGCAATGCGTTTTTTTATTAATTCACTAATTTCAGTTGAATTTAGTTGCATCTTCTAATCCTCTTATAATTGCAACTCATTTGC  >  2/117342‑117818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑