Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	93,348	A→G	A62A (GCA→GCG)	unknown →	CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase (EC 2.7.8.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	93,348	0	A	G	100.0%	209.7 / NA	76	A62A (GCA→GCG)	unknown	CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase (EC 2.7.8.8)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/34);  total (42/34)

CTAATTAAAATTCATTTACATTCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCAGGACAAGAAATTCTGGATGAAATTTATCGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGTGCTGCACATTAAAGGT  >  2/93108‑93588                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 cTAATTAAAATTCATTTACATTCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGaa                                                                                                                                                                                                                                         <  1:563566/250‑1 (MQ=255)  tAATTAAAATTCATTTACATTCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAAt                                                                                                                                                                                                                                       <  1:351157/251‑1 (MQ=255)                     ttCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGGAATTTAttgt                                                                                                                                                                                                                    >  1:511629/1‑248 (MQ=255)                     ttCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGAAAAGAAATTCtttgtggaaattattgt                                                                                                                                                                                                                    >  1:540515/1‑235 (MQ=255)                       cTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACaaaaa                                                                                                                                                                                                                                         >  1:835187/1‑225 (MQ=255)                       cTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGaa                                                                                                                                                                                                                                         <  1:360056/228‑1 (MQ=255)                            tCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGc                                                                                                                                                                                                             <  1:343502/251‑1 (MQ=255)                            tCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGc                                                                                                                                                                                                             <  1:734931/251‑1 (MQ=255)                                    tttttATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTT‑AAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTTTGGATGAAATTTATTGTGCGAAGCAGGATaaaa                                                                                                                                                                                                    >  1:754745/1‑250 (MQ=255)                                         ataGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAGTTTATTGTGCGAAGCAGGATAAACCGGa                                                                                                                                                                                                >  1:428292/1‑251 (MQ=255)                                         ataGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGa                                                                                                                                                                                                >  1:659303/1‑251 (MQ=255)                                              gaACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAAATCTGGATGAAATTTATTGTGCGAAGCAGGATAAAcc                                                                                                                                                                                                   >  1:122133/1‑243 (MQ=255)                                                   aaTTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGAt                                                                                                                                                                                                        >  1:366416/1‑233 (MQ=255)                                                   aaTTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAAcc                                                                                                                                                                                                   <  1:358345/238‑1 (MQ=255)                                                   aaTTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTgggaggg                                                                                                                                                                                      >  1:261976/1‑246 (MQ=255)                                                   aaTTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAAATGGAAGTg                                                                                                                                                                                      >  1:732457/1‑251 (MQ=255)                                                        aTGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAAt                                                                                                                                                                                 <  1:584151/251‑1 (MQ=255)                                                        aTGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAAATGGAAGTGAAAAt                                                                                                                                                                                 >  1:800015/1‑251 (MQ=255)                                                            ttACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAAcc                                                                                                                                                                                                   <  1:555020/229‑1 (MQ=255)                                                                 gATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGGGAAAATTTTAattga                                                                                                                                                                        >  1:17883/1‑251 (MQ=255)                                                                      aaTCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTgg                                                                                                                                                                    >  1:264956/1‑250 (MQ=255)                                                                      aaTCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTAT‑‑‑TAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTTGAAGGGAAAATTTTAATTGATTGGCAt                                                                                                                                                                 >  1:840141/1‑250 (MQ=255)                                                                        tCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGTTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCAt                                                                                                                                                                 <  1:429848/251‑1 (MQ=255)                                                                        tCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGTTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCAt                                                                                                                                                                 <  1:445243/251‑1 (MQ=255)                                                                             cGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGgc                                                                                                                                                            <  1:258674/251‑1 (MQ=255)                                                                             cGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGgc                                                                                                                                                            <  1:150159/251‑1 (MQ=255)                                                                                gcgcgcAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGGAATTCTGGATGAAATTTATTTTGCGAAGCAGGGTAAACCGGAAATGGAAGGGAAAATTTTTATTGATTGGCATCGGGcgca                                                                                                                                                         >  1:379910/1‑251 (MQ=255)                                                                                gcgcgcAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCg                                                                                                                                                               >  1:872530/1‑245 (MQ=255)                                                                                           tCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATttgt                                                                                                                                              >  1:51143/1‑251 (MQ=255)                                                                                            cTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATttgtt                                                                                                                                             >  1:659597/1‑251 (MQ=255)                                                                                                aaaGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTggg                                                                                                                                          <  1:593368/250‑1 (MQ=255)                                                                                                      tGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGgag                                                                                                                                   >  1:401278/1‑251 (MQ=255)                                                                                                       gCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGgaga                                                                                                                                  >  1:118725/1‑251 (MQ=255)                                                                                                        ccATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGgaga                                                                                                                                  <  1:67569/250‑1 (MQ=255)                                                                                                               tGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAAcc                                                                                                                                                                                                   >  1:579911/1‑178 (MQ=255)                                                                                                                 ccATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGGGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGcca                                                                                                                         >  1:65182/1‑250 (MQ=255)                                                                                                                   aTTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGccacca                                                                                                                      >  1:309393/1‑251 (MQ=255)                                                                                                                                cAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAAcc                                                                                                                                                                                                   >  1:196590/1‑161 (MQ=255)                                                                                                                                 aaGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGtac                                                                                                        <  1:611735/251‑1 (MQ=255)                                                                                                                                   gTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGGGAAGCAGGATAAAACGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGGGCGGAGAAAAGCGCCACCAATGCTGATTGGtacta                                                                                                      >  1:487230/1‑251 (MQ=255)                                                                                                                                   gTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAAACGGAACTGGAAGTGAAAATTTTAATTGATTGGCAACGGGCGCAACGCAATTTTTTGGGTGCGGAGCAAAGCGCCACCAATGCTGATTGGtacta                                                                                                      >  1:583666/1‑251 (MQ=255)                                                                                                                                      gAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAAc                                                                                                                                                                                              <  1:283467/160‑1 (MQ=255)                                                                                                                                      gAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGa                                                                                                   >  1:618105/1‑251 (MQ=255)                                                                                                                                       aaTTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGa                                                                                                   <  1:465067/250‑1 (MQ=255)                                                                                                                                       aaTTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGa                                                                                                   <  1:740464/250‑1 (MQ=255)                                                                                                                                         tttatttaTAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAAc                                                                                                <  1:154989/251‑1 (MQ=255)                                                                                                                                            atttatAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTAttg                                                                                                                                                                                                                     >  1:518791/1‑129 (MQ=255)                                                                                                                                            atttatAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTTTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAAATTTAATTGATTGGCATCGGGCGCAACGCAATTTGGTGGGGGCGGGGAAAAGCGCCACCAATGCTGTTTGGGACTACGAAACCCg                                                                                             >  1:550862/1‑251 (MQ=255)                                                                                                                                                           gCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAAACCGCCAGAAATATCaa                                                                                >  1:737039/1‑249 (MQ=255)                                                                                                                                                            ccTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAAttt                                                                                                                                                <  1:46186/184‑1 (MQ=255)                                                                                                                                                                      cGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATc                                                                   <  1:22409/251‑1 (MQ=255)                                                                                                                                                                        aaaTTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGGGCGGAGAAAAgcg                                                                                                                            >  1:366273/1‑192 (MQ=255)                                                                                                                                                                           ttattCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATttgtt                                                                                                                                             >  1:877220/1‑172 (MQ=255)                                                                                                                                                                             attCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGgag                                                                                                                                   >  1:330593/1‑180 (MQ=255)                                                                                                                                                                                  gCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGtt                                                         >  1:247162/1‑249 (MQ=255)                                                                                                                                                                                   cTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGttc                                                        <  1:446268/249‑1 (MQ=255)                                                                                                                                                                                      aCTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCgg                                                   <  1:560976/251‑1 (MQ=255)                                                                                                                                                                                                     aCGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCAt                                                                                                                                                                 <  1:275873/126‑1 (MQ=255)                                                                                                                                                                                                      cgcgGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACAcc                                   <  1:595474/251‑1 (MQ=255)                                                                                                                                                                                                      cgcgGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACAcc                                   <  1:845834/251‑1 (MQ=255)                                                                                                                                                                                                       gcgGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCata                                                             >  1:831093/1‑224 (MQ=255)                                                                                                                                                                                                        cgGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCcg                                 >  1:286991/1‑251 (MQ=255)                                                                                                                                                                                                             tattGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAg                            <  1:355807/251‑1 (MQ=255)                                                                                                                                                                                                             tattGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAg                            <  1:665799/251‑1 (MQ=255)                                                                                                                                                                                                              attGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGa                                                                     >  1:218768/1‑209 (MQ=255)                                                                                                                                                                                                              attGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAacac                                    <  1:163252/242‑1 (MQ=255)                                                                                                                                                                                                              attGTGACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGGACCGATTAACACCCGCGAAgg                           >  1:11290/1‑250 (MQ=255)                                                                                                                                                                                                                   gACCGCACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCg                      >  1:768765/1‑251 (MQ=255)                                                                                                                                                                                                                        cACTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAAc                                                                                                                                                                                              >  1:778082/1‑78 (MQ=255)                                                                                                                                                                                                                          cTTTATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGa                                                                                                   <  1:860575/167‑1 (MQ=255)                                                                                                                                                                                                                            ttATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGtgctg              >  1:495148/1‑250 (MQ=255)                                                                                                                                                                                                                            ttATTGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATGTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGtgctgc             <  1:188967/251‑1 (MQ=255)                                                                                                                                                                                                                                tGGCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGTGCTGCACAt         <  1:549124/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggCAAAATGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCa                                                                                 >  1:347419/1‑178 (MQ=255)                                                                                                                                                                                                                                      aaTGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGTGCTGCACATTAAAgg   <  1:831100/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTGACGAGGCGGGACAAGAAATTCTGGATGAAATTTATTGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGTGCTGCACATTAAAGGt  <  1:384251/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 CTAATTAAAATTCATTTACATTCTTACTCACAGCGTTTTTATAGAGAACTAATTTATGTTTACAGATAAAATCGAACGTGCGCGCAAACGTCTTGAAAGCCTGCCATGTTTGCCATTACAGCCGGAGCAAGTGGAATTTATTTATAGCCCGGCTGCCTTTAAAAGCGAAATTATTCAGCTGACTCGACAAGCAAAAACGCGGATTATTGTGACCGCACTTTATTGGCAAAATGACGAGGCAGGACAAGAAATTCTGGATGAAATTTATCGTGCGAAGCAGGATAAACCGGAACTGGAAGTGAAAATTTTAATTGATTGGCATCGGGCGCAACGCAATTTGTTGGGTGCGGAGAAAAGCGCCACCAATGCTGATTGGTACTACGAAACCCGCCAGAAATATCAACTCGCGGAAGATCCGCATATGTTCTTCGGCGTACCGATTAACACCCGCGAAGTGTTCGGCGTGCTGCACATTAAAGGT  >  2/93108‑93588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑