Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	332,713	C→T	S11F (TCC→TTC)	unknown →	Mn2+ and Fe2+ transporters of the NRAMP family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	332,713	0	C	T	100.0%	113.5 / NA	43	S11F (TCC→TTC)	unknown	Mn2+ and Fe2+ transporters of the NRAMP family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

TGCCGGTTATTTATTTTTTATCTTAATATTATTTCCTGAAATTATTAAATAATCAGAGTTTTATTCTGCTATTTTCTCTGAGGTAGAATTTTATTTTCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATCCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAGGGTATCGCCAAAAAGGGCGTTTCTATT  >  1/332510‑332952                                                                                                                                                                                                            |                                                                                                                                                                                                                                                tGCCGGTTATTTATTTTTTATCTTAATATTATTTCCTGAAATTATTAAATAATCAGAGTTTTATTCTGCTATTTTCTCTGAGGTAGAATTTTATTTTCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATgg                                                                                                                                                                                                  <  1:249026/251‑1 (MQ=255)                                                                           tctcTGAGGTAGAATTTTATTTTCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTg                                                                                                                        <  1:755800/251‑1 (MQ=255)                                                                           tctcTGAGGTAGAATTTTATTTTCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTg                                                                                                                        <  1:491915/251‑1 (MQ=255)                                                                                        attttattttCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATcc                                                                                                           <  1:366687/251‑1 (MQ=255)                                                                                                  cTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAAttt                                                                                                 <  1:640517/251‑1 (MQ=255)                                                                                                              gTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATcc                                                                                                           <  1:133418/229‑1 (MQ=255)                                                                                                                      cTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCAtattat                                                                                                                                                                   <  1:558845/165‑1 (MQ=255)                                                                                                                      cTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTc                                                                              >  1:115955/1‑250 (MQ=255)                                                                                                                        attatAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGcc                                                                                                                                                   >  1:47903/1‑179 (MQ=255)                                                                                                                        attatAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGa                                                                           >  1:413391/1‑251 (MQ=255)                                                                                                                               aTGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGgtg                                                                    <  1:865850/251‑1 (MQ=255)                                                                                                                                           gCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGAtt                                                                         >  1:875721/1‑234 (MQ=255)                                                                                                                                            cATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCCGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGcc                                                                                                                                                   >  1:405097/1‑159 (MQ=255)                                                                                                                                              tttttATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTgg                                                     >  1:794570/1‑251 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCAc                                                                                                                                                                                                                                              >  1:148949/1‑54 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATcc                                                                                                           >  1:488576/1‑185 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCaa                                                               <  1:543453/229‑1 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATa                                         >  1:367541/1‑251 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATa                                         >  1:838354/1‑251 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATa                                         >  1:819500/1‑251 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAGCGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGAGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGCTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACggg                                              >  1:739511/1‑246 (MQ=255)                                                                                                                                                          aTTTATAAGAGGATATTTTATGAATGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCGTTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGGGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCtt                                                       <  1:491407/237‑1 (MQ=255)                                                                                                                                                           tttATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGt                                                                                                                                    <  1:865814/159‑1 (MQ=255)                                                                                                                                                           tttATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATcc                                                                                                           <  1:420325/184‑1 (MQ=255)                                                                                                                                                                 agagGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAAttt                                                                                                                                         >  1:445370/1‑148 (MQ=255)                                                                                                                                                                  gagGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCttgttg                                 >  1:364507/1‑251 (MQ=255)                                                                                                                                                                   agGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGt                                                                                                                                    <  1:309678/151‑1 (MQ=255)                                                                                                                                                                   agGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGGAATACCttgttg                                 <  1:125272/250‑1 (MQ=255)                                                                                                                                                                   agGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCACTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCttgttg                                 <  1:435145/250‑1 (MQ=255)                                                                                                                                                                    ggATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGTGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAgctgc                                                                                                                                                                                        <  1:238716/98‑1 (MQ=255)                                                                                                                                                                    ggATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGa                               >  1:290029/1‑251 (MQ=255)                                                                                                                                                                    ggATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGa                               >  1:780639/1‑251 (MQ=255)                                                                                                                                                                       tatTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAgg                            >  1:684654/1‑251 (MQ=255)                                                                                                                                                                       tatTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTCAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATAc                                                          <  1:701958/221‑1 (MQ=255)                                                                                                                                                                        atTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAggg                           <  1:782702/251‑1 (MQ=255)                                                                                                                                                                        atTTTATGAGTGAAGTGTCAAATCTGGTAGAAAAATTCACTTGGGCGATTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAggg                           <  1:769419/251‑1 (MQ=255)                                                                                                                                                                         ttttATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGt                                                                                                                                    >  1:538006/1‑145 (MQ=255)                                                                                                                                                                         ttttATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATcc                                                                                                           >  1:307058/1‑170 (MQ=255)                                                                                                                                                                         ttttATGAGTGAAGTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAGGGt                          >  1:75430/1‑251 (MQ=255)                                                                                                                                                                                      gTTTCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTa                                                                                                              <  1:445383/154‑1 (MQ=255)                                                                                                                                                                                       tttCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATgg                                                                                                                                                                                                  >  1:305312/1‑69 (MQ=255)                                                                                                                                                                                       tttCAAATCTGGTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGc                                             >  1:720702/1‑218 (MQ=255)                                                                                                                                                                                                  gTAGAAAAATTCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAGGGTATCGCCAAAAAGGGCGTTTCTAtt  <  1:287550/250‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                TGCCGGTTATTTATTTTTTATCTTAATATTATTTCCTGAAATTATTAAATAATCAGAGTTTTATTCTGCTATTTTCTCTGAGGTAGAATTTTATTTTCTTTACTCAATTGTTAAATTCTATTATAAATGGTTTCCATTGCATTTTTATGGTCAATTTATAAGAGGATATTTTATGAGTGAAGTTTCAAATCTGGTAGAAAAATCCACTTGGGCGAGTAAATTTTCTGCCTTAGGTCCGGGCATTGTCATGGCGTCAGCTGCTGTCGGTGGTTCGCATATTATCGCTTCAACCCAAGCCGGTGCAATTTATGGTTGGGAGTTGGTGAGCATTGTTATCCTGGTTAATTTATTCAAATATCCTTTTTTCCGATTTGGTGTGCAATATACCTTGGATACGGGCAATACCTTGTTGGAAGGGTATCGCCAAAAAGGGCGTTTCTATT  >  1/332510‑332952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑