Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	331,816	C→T	P435P (CCC→CCT)	unknown →	Aspartyl‑tRNA synthetase (EC 6.1.1.12)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	331,816	0	C	T	100.0%	215.5 / NA	79	P435P (CCC→CCT)	unknown	Aspartyl‑tRNA synthetase (EC 6.1.1.12)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/32);  total (47/32)

ACGTAAACGCCGGTCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCCATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGAACAACAACGCGAAAAATTCGGC  >  1/331581‑332059                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aCGTAAACGCCGGTCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgat                                                                                                                                                                                                                                      >  1:727793/1‑251 (MQ=255)  cGTAAACGCCGGTCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatg                                                                                                                                                                                                                                     >  1:275814/1‑251 (MQ=255)     aaaCGCCGGTCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatgatg                                                                                                                                                                                                                                  <  1:699580/251‑1 (MQ=255)      accGCCGGTCTTGAAGGCGTCCAAAGCTCTATTGAAAAATTCTTAAATGAAGCCGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAGCTGGCGATATTTTATTTTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatgatga                                                                                                                                                                                                                                 <  1:738721/249‑1 (MQ=255)              tCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatgatg                                                                                                                                                                                                                                  <  1:228509/242‑1 (MQ=255)                             aaGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGcac                                                                                                                                                                                                           >  1:115741/1‑250 (MQ=255)                               gCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGcacca                                                                                                                                                                                                         >  1:596009/1‑250 (MQ=255)                                        cAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGACCGCGTGGGCGCGCAAACTGGCGATTTTTTATTCTTCGGTGCCGAAAAAGGGAAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTACGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAc                                                                                                                                                                                                <  1:323791/250‑1 (MQ=255)                                        cAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGAAAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTGACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAc                                                                                                                                                                                                <  1:478213/250‑1 (MQ=255)                                        aaaaaTTCTTAAATGAAGACGTGCAAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAcc                                                                                                                                                                                               <  1:763466/250‑1 (MQ=255)                                             ttCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGTACGTGATGATGAAGGCTGCTTGACGGCAATGCaccacc                                                                                                                                                                                                       >  1:168482/1‑238 (MQ=255)                                             ttCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAcc                                                                                                                                                                                               <  1:661934/246‑1 (MQ=255)                                                              gCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCacac                                                                                                                                                                            >  1:258725/1‑248 (MQ=255)                                                               cTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatgatg                                                                                                                                                                                                                                  >  1:489250/1‑193 (MQ=255)                                                               cTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGa                                                                                                                                                                        >  1:544533/1‑251 (MQ=255)                                                               cTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGa                                                                                                                                                                        >  1:364403/1‑251 (MQ=255)                                                                        ttGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAcc                                                                                                                                                                                               >  1:129079/1‑219 (MQ=255)                                                                          ttttGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTACCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAg                                                                                                                                                             <  1:111631/250‑1 (MQ=255)                                                                          gTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAg                                                                                                                                                             >  1:19517/1‑251 (MQ=255)                                                                          gTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAg                                                                                                                                                             >  1:169329/1‑251 (MQ=255)                                                                          gTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAg                                                                                                                                                             >  1:579034/1‑251 (MQ=255)                                                                                               aaCTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCg                                                                                                                                        >  1:194688/1‑251 (MQ=255)                                                                                                       atatTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTc                                                                                                                                                                                                  >  1:874828/1‑185 (MQ=255)                                                                                                       atatTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGc                                                                                                                                 <  1:535893/250‑1 (MQ=255)                                                                                                       atatTTTATTCTTCGGTGCCGACAAATGGAAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGc                                                                                                                                 <  1:306991/250‑1 (MQ=255)                                                                                                         atTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGc                                                                                                                                                                                                                  <  1:14743/167‑1 (MQ=255)                                                                                                             tATTCTTCGGTGCCGAAAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGat                                                                                                                          <  1:893327/251‑1 (MQ=255)                                                                                                             tATTCTTCGGTGCCAACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGat                                                                                                                          <  1:604108/251‑1 (MQ=255)                                                                                                               ttcttcGGTGCCGACAAAGGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGatat                                                                                                                        <  1:6456/251‑1 (MQ=255)                                                                                                                  ttcGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGt                                                                                                                     <  1:539170/251‑1 (MQ=255)                                                                                                                      gTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGtcat                                                                                                                  >  1:830721/1‑250 (MQ=255)                                                                                                                        gCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGa                                                                                                                           >  1:521644/1‑239 (MQ=255)                                                                                                                            aCAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATgg                                                                                                            <  1:5973/250‑1 (MQ=255)                                                                                                                                    cAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAg                                                                                                    <  1:248301/250‑1 (MQ=255)                                                                                                                                    cAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAg                                                                                                    <  1:796330/250‑1 (MQ=255)                                                                                                                                    cAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAg                                                                                                    <  1:298939/250‑1 (MQ=255)                                                                                                                                    cAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAg                                                                                                    <  1:341003/250‑1 (MQ=255)                                                                                                                                        ccGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAgtgggt                                                                                               >  1:487235/1‑251 (MQ=255)                                                                                                                                        ccGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAg                                                                                                    >  1:887545/1‑246 (MQ=255)                                                                                                                                          gCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCAc                                                                                                                                                                                                <  1:120638/152‑1 (MQ=255)                                                                                                                                           ccACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACg                                                                                                                            >  1:752981/1‑219 (MQ=255)                                                                                                                                           ccACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGc                                                                                            <  1:95502/251‑1 (MQ=255)                                                                                                                                                gATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATgg                                                                                                            >  1:400118/1‑230 (MQ=255)                                                                                                                                                 aTTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCgg                                                                                          <  1:767721/247‑1 (MQ=255)                                                                                                                                                  ttCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAgtgggt                                                                                               <  1:500976/241‑1 (MQ=255)                                                                                                                                                  ttCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCg                                                                                     >  1:554801/1‑251 (MQ=255)                                                                                                                                                    cAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTg                                                                                   >  1:616894/1‑251 (MQ=255)                                                                                                                                                            gcgTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTAtttt                                                                           >  1:636096/1‑251 (MQ=255)                                                                                                                                                                 gcGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGAt                                                                       >  1:371234/2‑250 (MQ=255)                                                                                                                                                                 aCGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGtgatgatga                                                                                                                                                                                                                                 >  1:53121/1‑96 (MQ=255)                                                                                                                                                                 aCGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGt                                                                                                                     >  1:523219/1‑204 (MQ=255)                                                                                                                                                                 aCGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGAt                                                                       >  1:745839/1‑250 (MQ=255)                                                                                                                                                                 aCGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATc                                                                      <  1:320721/251‑1 (MQ=255)                                                                                                                                                                 aCGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATc                                                                      <  1:77664/251‑1 (MQ=255)                                                                                                                                                                  cGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTg                                                                         >  1:606709/1‑247 (MQ=255)                                                                                                                                                                        aaaTTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAAt                                                               >  1:355422/1‑251 (MQ=255)                                                                                                                                                                        aaaTTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAAt                                                               >  1:269696/1‑251 (MQ=255)                                                                                                                                                                         aaTTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATg                                                              >  1:1578/1‑251 (MQ=255)                                                                                                                                                                                     aTCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGa                                                                                                      >  1:359154/1‑199 (MQ=255)                                                                                                                                                                                     aTCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCgtg                                                  >  1:605061/1‑251 (MQ=255)                                                                                                                                                                                     aTCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCgtg                                                  >  1:113875/1‑251 (MQ=255)                                                                                                                                                                                     aTCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCgtg                                                  >  1:493685/1‑251 (MQ=255)                                                                                                                                                                                     aTCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCgtg                                                  >  1:866366/1‑251 (MQ=255)                                                                                                                                                                                        tcGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTc                                               <  1:559586/251‑1 (MQ=255)                                                                                                                                                                                        tcGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTc                                               <  1:625497/251‑1 (MQ=255)                                                                                                                                                                                          ggTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGc                                                             >  1:355012/1‑235 (MQ=255)                                                                                                                                                                                          ggTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCg                                             >  1:392157/1‑251 (MQ=255)                                                                                                                                                                                          ggTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCg                                             >  1:137244/1‑251 (MQ=255)                                                                                                                                                                                                aCCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACg                                                                                                                                                                                                                                          >  1:55271/1‑56 (MQ=255)                                                                                                                                                                                                   cGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCgg                                    >  1:154047/1‑251 (MQ=255)                                                                                                                                                                                                         gATGAATGGCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATc                                                                      >  1:387954/1‑211 (MQ=255)                                                                                                                                                                                                                ggCAACCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTcac                                                                                                                                                                              <  1:33447/100‑1 (MQ=255)                                                                                                                                                                                                                    aCCGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGaacaac                   <  1:322018/251‑1 (MQ=255)                                                                                                                                                                                                                      cGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCTAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGaacaacaa                 <  1:441348/251‑1 (MQ=255)                                                                                                                                                                                                                      cGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTAtttt                                                                           >  1:166998/1‑193 (MQ=255)                                                                                                                                                                                                                      cGCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGaacaacaa                 >  1:804454/1‑251 (MQ=255)                                                                                                                                                                                                                       gCTTTGGGTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGaacaacaa                 >  1:306251/1‑250 (MQ=255)                                                                                                                                                                                                                            gggTTATTGATTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGAACAACAACGCGaa           >  1:896310/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTTTCCTATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGAACAACAACGCGAAAAATTCGGc  <  1:482357/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ACGTAAACGCCGGTCTTGAAGGCGTCCAAAGCCCTATTGCAAAATTCTTAAATGAAGACGTGCTAAAAGCCTTGTTTGAACGCGTGGGCGCGCAAACTGGCGATATTTTATTCTTCGGTGCCGACAAATGGCAAACCGCCACAGATTCAATGGGCGCGTTACGTTTGAAATTAGGACGCGATCTCGGTTTAACCCGTTTAGATGAATGGCAACCGCTTTGGGTTATTGATTTTCCCATGTTTGAACGTGATGATGAAGGCTGCTTGACGGCAATGCACCACCCATTCACCTCGCCAAAAGATTTCACACCTGAACAATTAGAAGCGGATCCGACAAGTGCGGTCGCCAATGCTTACGATATGGTCATCAATGGTTATGAAGTGGGTGGCGGTTCCGTGCGTATTTTTGATCCGAAAATGCAACAAACCGTGTTCCGCATTCTCGGTATTGACGAAGAACAACAACGCGAAAAATTCGGC  >  1/331581‑332059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑