BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	77,788	A→G	V136A (GTG→GCG)	unknown ←	tRNA pseudouridine(13) synthase (EC 5.4.99.27)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	77,788	0	A	G	100.0%	332.5 / NA	119	V136A (GTG→GCG)	unknown	tRNA pseudouridine(13) synthase (EC 5.4.99.27)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (57/62); total (57/62)

Only 100 of 119 total aligned reads displayed.

GCTGCGCCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCACGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGCCTACAAAC  >  2/77549‑78033

gctgcGCCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCgcag                                                                                                                                                                                                                                             >  1:21495/1‑250 (MQ=255)

  tgcGCCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaa                                                                                                                                                                                                                                          <  1:408235/251‑1 (MQ=255)

  tgcGCCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaa                                                                                                                                                                                                                                          <  1:312006/251‑1 (MQ=255)

   gcgcCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaaa                                                                                                                                                                                                                                         >  1:118850/1‑251 (MQ=255)

   gcgcCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaaa                                                                                                                                                                                                                                         >  1:898437/1‑251 (MQ=255)

   gcgcCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaaa                                                                                                                                                                                                                                         >  1:811078/1‑251 (MQ=255)

   gcgcCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCaaa                                                                                                                                                                                                                                         >  1:54158/1‑251 (MQ=255)

            tGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCaaaa                                                                                                                                                                                                                                <  1:460663/251‑1 (MQ=255)

               gTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGa                                                                                                                                                                                                                             >  1:470718/1‑251 (MQ=255)

                      aCGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGACATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCtt                                                                                                                                                                                                                      >  1:245428/1‑251 (MQ=255)

                       cGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTg                                                                                                                                                                                                                     >  1:796352/1‑251 (MQ=255)

                             gCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGc                                                                                                                                                                                                                <  1:327148/250‑1 (MQ=255)

                                 cGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCt                                                                                                                                                                                                                       <  1:467070/239‑1 (MQ=255)

                                 cGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCg                                                                                                                                                                                                           >  1:790204/1‑251 (MQ=255)

                                 cGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCg                                                                                                                                                                                                           >  1:310968/1‑251 (MQ=255)

                                   aTTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAg                                                                                                                                                                                                                  >  1:728175/1‑242 (MQ=255)

                                   aTTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGa                                                                                                                                                                                                          <  1:153731/250‑1 (MQ=255)

                                   aTTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGAt                                                                                                                                                                                                         >  1:377586/1‑251 (MQ=255)

                                       aaTTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACgg                                                                                                                                                                                                     >  1:325620/1‑251 (MQ=255)

                                       aaTTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACgg                                                                                                                                                                                                     <  1:66256/251‑1 (MQ=255)

                                        cacccaagccctacgctgggcggCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGC‑AAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGAt                                                                                                                                                                                                   >  1:284566/18‑251 (MQ=255)

                                         ttGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGAt                                                                                                                                                                                                   <  1:608996/251‑1 (MQ=255)

                                         ttGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGAt                                                                                                                                                                                                   <  1:835543/251‑1 (MQ=255)

                                               aTCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCg                                                                                                                                                                                             >  1:531053/1‑251 (MQ=255)

                                               aTCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCg                                                                                                                                                                                             <  1:332623/251‑1 (MQ=255)

                                               aTCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCg                                                                                                                                                                                             <  1:739324/251‑1 (MQ=255)

                                               aTCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCg                                                                                                                                                                                             >  1:104988/1‑251 (MQ=255)

                                                    acgctgggcggCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTtggt                                                                                                                                                                                        >  1:741129/6‑251 (MQ=255)

                                                                     tataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGcc                                                                                                                                                                                                                        <  1:742772/202‑1 (MQ=255)

                                                                     tataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAg                                                                                                                                                                                                                  >  1:887257/1‑208 (MQ=255)

                                                                     tataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGa                                                                                                                                                                       >  1:85985/1‑251 (MQ=255)

                                                                      ataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:209398/251‑1 (MQ=255)

                                                                      ataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:526216/251‑1 (MQ=255)

                                                                      ataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:529675/251‑1 (MQ=255)

                                                                      ataGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      >  1:817023/1‑251 (MQ=255)

                                                                          aaaCTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATc                                                                                                                                                                  <  1:840854/251‑1 (MQ=255)

                                                                           aaCTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATcc                                                                                                                                                                 <  1:484134/251‑1 (MQ=255)

                                                                               gcgcTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAAt                                                                                                                                                                                                                               <  1:643078/185‑1 (MQ=255)

                                                                                cgcTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGcc                                                                                                                                                            <  1:632840/251‑1 (MQ=255)

                                                                                       tgCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAgg                                                                                                                                                     <  1:304786/251‑1 (MQ=255)

                                                                                           aTCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATcc                                                                                                                                                                 <  1:329372/235‑1 (MQ=255)

                                                                                            tCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGaaa                                                                                                                                                >  1:125092/1‑251 (MQ=255)

                                                                                                 cACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGc                                                                                                                                           <  1:48373/251‑1 (MQ=255)

                                                                                                   cTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTTAAGAATAACCACGCCGTCCAGGCGAAATTgg                                                                                                                                            >  1:680999/1‑248 (MQ=255)

                                                                                                                  ccgccCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      >  1:134579/1‑207 (MQ=255)

                                                                                                                    gccCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTg                                                                                                                        >  1:392670/1‑251 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGCTTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCgg                                                                                                                   >  1:425266/1‑251 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:647847/200‑1 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATcc                                                                                                                                                                 >  1:880026/1‑205 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTc                                                                                                                                                         >  1:581075/1‑213 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCgg                                                                                                                   >  1:717590/1‑251 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCgg                                                                                                                   >  1:791854/1‑251 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCgg                                                                                                                   >  1:834634/1‑251 (MQ=255)

                                                                                                                         gCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGACGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCgg                                                                                                                   >  1:773263/1‑251 (MQ=255)

                                                                                                                                 cTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGc                                                                                                           <  1:761276/251‑1 (MQ=255)

                                                                                                                                    gggTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAgg                                                                                                        >  1:26682/1‑251 (MQ=255)

                                                                                                                                    gggTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGGCACTTCAAGAATATCCACGCCGTCAAGGTGAAAt                                                                                                                                               >  1:37285/1‑212 (MQ=255)

                                                                                                                                      gTGAGATTGTTCCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGc                                                                                                       <  1:316516/250‑1 (MQ=255)

                                                                                                                                          gaTTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATcc                                                                                                                                                                 >  1:146386/1‑188 (MQ=255)

                                                                                                                                           aTTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAAc                                                                                                  <  1:796380/250‑1 (MQ=255)

                                                                                                                                             tgttgCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCAt                                                                                               <  1:150632/251‑1 (MQ=255)

                                                                                                                                                  ccGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGtt                                                                                           <  1:4273/250‑1 (MQ=255)

                                                                                                                                                  ccGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTc                                                                                          <  1:706582/251‑1 (MQ=255)

                                                                                                                                                  ccGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTc                                                                                          <  1:26433/251‑1 (MQ=255)

                                                                                                                                                                 cGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:337233/160‑1 (MQ=255)

                                                                                                                                                                   cTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGAt                                                                         >  1:723938/1‑251 (MQ=255)

                                                                                                                                                                   cTGTTCGGTGAAATAATCGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGAt                                                                         <  1:120059/251‑1 (MQ=255)

                                                                                                                                                                     gTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGc                                                                                                       >  1:466141/1‑219 (MQ=255)

                                                                                                                                                                     gTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGAt                                                                         >  1:119008/1‑249 (MQ=255)

                                                                                                                                                                          gTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      <  1:22444/151‑1 (MQ=255)

                                                                                                                                                                          gTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGc                                                                                                                                                             <  1:19763/160‑1 (MQ=255)

                                                                                                                                                                          gTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCAt                                                                                               >  1:729066/1‑222 (MQ=255)

                                                                                                                                                                          gTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCaa                                                                  >  1:513893/1‑251 (MQ=255)

                                                                                                                                                                          gTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGGGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCaa                                                                  >  1:780429/1‑251 (MQ=255)

                                                                                                                                                                             aaaTAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAAc                                                                <  1:510402/250‑1 (MQ=255)

                                                                                                                                                                             aaaTAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAAc                                                                >  1:715526/1‑250 (MQ=255)

                                                                                                                                                                               ataatTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGa                                                                          <  1:189269/238‑1 (MQ=255)

                                                                                                                                                                                taatTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGaa                                                                                                                                                                      >  1:538240/1‑145 (MQ=255)

                                                                                                                                                                                        gAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTc                                                     <  1:393789/250‑1 (MQ=255)

                                                                                                                                                                                        gAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTc                                                     <  1:167622/250‑1 (MQ=255)

                                                                                                                                                                                        gAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTc                                                     <  1:143997/250‑1 (MQ=255)

                                                                                                                                                                                        gAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCGCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTGGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTc                                                     <  1:682414/250‑1 (MQ=255)

                                                                                                                                                                                            ccGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGt                                                 <  1:408009/250‑1 (MQ=255)

                                                                                                                                                                                            ccGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGt                                                 <  1:813085/250‑1 (MQ=255)

                                                                                                                                                                                             cGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               <  1:703398/251‑1 (MQ=255)

                                                                                                                                                                                             cGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               <  1:308593/251‑1 (MQ=255)

                                                                                                                                                                                             cGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               <  1:625584/251‑1 (MQ=255)

                                                                                                                                                                                             cGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               <  1:568174/251‑1 (MQ=255)

                                                                                                                                                                                             cGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATCGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               <  1:323550/251‑1 (MQ=255)

                                                                                                                                                                                                         gAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATg                                   >  1:877726/1‑251 (MQ=255)

                                                                                                                                                                                                             aTCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAAt                                                                                                                                     >  1:636021/1‑149 (MQ=255)

                                                                                                                                                                                                                        cTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGt                                                 >  1:893257/1‑222 (MQ=255)

                                                                                                                                                                                                                        cTTTGAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCAGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGc                     >  1:861774/1‑250 (MQ=255)

                                                                                                                                                                                                                            gAGCTCGTCGGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGa                                                                                     <  1:651521/182‑1 (MQ=255)

                                                                                                                                                                                                                                   tcgGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGc          >  1:493527/1‑250 (MQ=255)

                                                                                                                                                                                                                                    cgGTTTCTTCCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCAGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGc          >  1:435910/1‑249 (MQ=255)

                                                                                                                                                                                                                                        ttcttcCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGCCTacaa    <  1:29304/251‑1 (MQ=255)

                                                                                                                                                                                                                                          cttcCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTg                                               >  1:69062/1‑206 (MQ=255)

                                                                                                                                                                                                                                          cttcCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGCCTacaaac  <  1:298068/251‑1 (MQ=255)

                                                                                                                                                                                                                                          cttcCGCGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGCCTacaaac  <  1:621912/251‑1 (MQ=255)

GCTGCGCCAGACTGAGTTCAATACGTTTGGCAACGATTAAATTGAAAATCTCACTGCGGGCGGCGGAAATATAGAAACTGCGCTTGTTGCGATCTTTCACTTTGATTTCGCCCGCCGCCCAGCGTAGGGCTTGGGTGAGATTGTTGCCGTCGCGCCCGAAACGCTGTTCGGTGAAATAATTGGGGAAGCCGTTTTTTGCCAGAAAATCCAACCGCACTTTGAGCTCGTCGGTTTCTTCCACGTGGCGCAGCAAAATCTCAAAATGATTGCCTTGCAGGCTGCCGATACGGATTTTGCGTTGGTGGCGGGTCACTTCAAGAATATCCACGCCGTCAAGGTGAAATTGGCTGAAATCCGGCGTCGGTTGCCCGGGCATTTGCAGGCTGAACCATTGTTCGGTGACAGCTTTGCGATCTTTCAAACCGGCATAACTCATGTTGCGTGCCGAAATGCCGGCGAATTTCGCCAGTTGCTCGCCTACAAAC  >  2/77549‑78033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`