Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	330,290	A→G	N5N (AAT→AAC)	unknown ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	330,290	0	A	G	100.0%	58.7 / NA	22	N5N (AAT→AAC)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/14);  total (8/14)

AGAGAAAATCAAGATTATTAATCCAATCTGAGATTCTTTATTAAAAATAACTTGATTGTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATATTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGTATAATATCTCTTGTAATAAGAAATATAATTTTTATTGATAGGTATTTTAAGTTACTATATTTTTTCGCATACTACAT  >  1/330062‑330486                                                                                                                                                                                                                                     |                                                                                                                                                                                                     agagaAAATCAAGATTATTAATCCAATCTGAGATTCTTTATTAAAAATAACTTGATTGTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGc                                                                                                                                                                                  <  1:627687/249‑1 (MQ=255)                           tCTGAGATTCTTTATTAAAAATAACTTGATTGTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGa                                                                                                                                                      <  1:470797/251‑1 (MQ=255)                                    cTTTATTAAAAATAACTTGATTGTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAgg                                                                                                                                             <  1:839946/251‑1 (MQ=255)                                                          gTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGa                                                                                                                                                      <  1:880868/220‑1 (MQ=255)                                                                ggCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAg                                                                                                                  <  1:536972/250‑1 (MQ=255)                                                                        tAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTc                                                                                                          <  1:228568/250‑1 (MQ=255)                                                                                              aaTATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGc                                                                                                                                                                                  <  1:852751/156‑1 (MQ=255)                                                                                                 atGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGc                                                                                                                                                                                  <  1:498560/153‑1 (MQ=255)                                                                                                 atGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCt                                                                                                                                                           >  1:549643/1‑176 (MQ=255)                                                                                                         ctGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGTATAatat                                                                        >  1:498093/1‑251 (MQ=255)                                                                                                                                   ccATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGTATAATAtct                                                                      <  1:259242/227‑1 (MQ=255)                                                                                                                                     atatTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGc                                                                                                                                                                                  >  1:462548/1‑117 (MQ=255)                                                                                                                                     atatTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACaaa                                                                                                                                               >  1:50478/1‑152 (MQ=255)                                                                                                                                     atatTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTaaga                                                                                                  >  1:556619/1‑197 (MQ=255)                                                                                                                                                                aTTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGCAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTaagcag                                                                                                >  1:432370/1‑169 (MQ=255)                                                                                                                                                                               gATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGTATAATATCTCTTGTAATAAGAAATATAATTTTTATTGATAGGTATTTTAAGTTACTATATTTTTTCGCATACTACAt  >  1:492341/1‑251 (MQ=255)                                                                                                                                                                                   gAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGtat                                                                             <  1:434992/172‑1 (MQ=255)                                                                                                                                                                                               gTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATg                                                                                                                    <  1:680513/121‑1 (MQ=255)                                                                                                                                                                                               gTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGtat                                                                             >  1:790091/1‑160 (MQ=255)                                                                                                                                                                                                        cGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGt                                                                                                                                      <  1:410812/94‑1 (MQ=255)                                                                                                                                                                                                        cGTAATAATAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGtat                                                                             <  1:828710/151‑1 (MQ=255)                                                                                                                                                                                                          taataaTAGAGCTAACGCAAAGAAAATGTTAATAAGTAGCATAAAGGCTATCCTTTTTAAg                                                                                                                                                                     <  1:697995/61‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                     AGAGAAAATCAAGATTATTAATCCAATCTGAGATTCTTTATTAAAAATAACTTGATTGTAATTGGCTTCAGTAATACATGAAAGATAGAATAAAATATGTACTACTGATAAGGCAATTGAATTTTTCTTTCCATATTGTATGGCGCCTTTTTTTAGTAAATTTTTTTCATTTCTGATTGAAATAGATAAAGTATAAAATCGTAATAATAGAGCTAACGCAAAGAAAATATTAATAAGTAGCATAAAGGCTATCCTTTTTAAGTGATATGTCTTGTGATAACAAAGGGTTATGTTTATTTTATCGTTCTATGAGTACAACTCATGTAAGAAGCTGATAATGACTATAGTATAATATCTCTTGTAATAAGAAATATAATTTTTATTGATAGGTATTTTAAGTTACTATATTTTTTCGCATACTACAT  >  1/330062‑330486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑