Predicted mutation
evidence seq id position mutation annotation gene description
RA 2 52,912 A→G D45D (GAT→GAC)  unknown ← Rod shape‑determining protein MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*252,9120AG93.5% 255.9 / 4.3 107D45D (GAT→GAC) unknownRod shape‑determining protein MreB
Reads supporting (aligned to +/- strand):  ref base A (0/7);  new base G (39/61);  total (39/68)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.91e-01

Only 100 of 107 total aligned reads displayed.

CGCGACGTTCTACTTGGGTGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAACAGAAAATTTTGTATAAATA  >  2/52671‑53158
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
              tGGGTGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGa                                                                                                                                                                                                                                  >  1:321416/1‑251 (MQ=255)
                ggTGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGaa                                                                                                                                                                                                                                 <  1:57516/250‑1 (MQ=255)
                  tGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAgg                                                                                                                                                                                                                               <  1:491193/250‑1 (MQ=255)
                  tGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAgg                                                                                                                                                                                                                               <  1:279540/250‑1 (MQ=255)
                            tcgagataggagtccgtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGa                                                                                                                                                                                                                    <  1:837279/232‑1 (MQ=255)
                            cGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGa                                                                                                                                                                                                                    <  1:398290/251‑1 (MQ=255)
                             cgagatgctcaggagtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:343383/233‑1 (MQ=255)
                             cgagatgctcaggagtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:732361/233‑1 (MQ=255)
                             cgagataggagtccgtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:117828/233‑1 (MQ=255)
                             cgagataggagtccgtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:157420/233‑1 (MQ=255)
                                     gagtccgtctcgTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGccc                                                                                                                                                                                                            <  1:795737/240‑1 (MQ=255)
                                     aaTTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:279793/243‑1 (MQ=255)
                                     aaTTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGcc                                                                                                                                                                                                             >  1:205545/1‑249 (MQ=255)
                                        tAATGCGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCATCATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGAc                                                                                                                                                                                                        <  1:639725/251‑1 (MQ=255)
                                                 tGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACat                                                                                                                                                                                               <  1:293944/251‑1 (MQ=255)
                                                 tGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACa                                                                                                                                                                                                <  1:534748/250‑1 (MQ=255)
                                                   ggCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGaa                                                                                                                                                                                                                                 <  1:703325/215‑1 (MQ=255)
                                                   ggCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACataa                                                                                                                                                                                             >  1:829908/1‑251 (MQ=255)
                                                    gCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATACTTTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:738122/229‑1 (MQ=255)
                                                      tCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACataaata                                                                                                                                                                                          <  1:381243/251‑1 (MQ=255)
                                                          aCGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAGCCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTTCTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAgtg                                                                                                                                                                                      <  1:513295/251‑1 (MQ=255)
                                                          aCGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATACCCTGACCTTTTACATAAATAAggg                                                                                                                                                                                      <  1:459001/251‑3 (MQ=255)
                                                          aCGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATACCCTGACCTTTTACATAAATAAggg                                                                                                                                                                                      <  1:801111/251‑3 (MQ=255)
                                                          aCGCATGAAATTGACGCTGTGCACTTGTTTAATGAATTACTTCAACATTTTTTCGGTGACGAAGAAGGCGGCGATAACCCCGTCTTTCAACGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCACTTCTTTTCCGGCGGCGGCAATAC‑TTTTTAGGGATCCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAg                                                                                                                                                                                        <  1:116573/249‑1 (MQ=255)
                                                           cGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAgtg                                                                                                                                                                                      >  1:234731/1‑250 (MQ=255)
                                                             cATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   >  1:556766/1‑219 (MQ=255)
                                                             cATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAgtgtg                                                                                                                                                                                    <  1:93616/250‑1 (MQ=255)
                                                                 aaaTTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGc                                                                                                                                                                               <  1:513895/251‑1 (MQ=255)
                                                                 aaaTTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGc                                                                                                                                                                               <  1:193164/251‑1 (MQ=255)
                                                                    ttGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGt                                                                                                                                                                            <  1:606489/251‑1 (MQ=255)
                                                                        cGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTAccc                                                                                                                                                                        <  1:370103/251‑1 (MQ=255)
                                                                         gCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCtactac                                                                                                                                                                                                                                     >  1:326053/1‑189 (MQ=255)
                                                                            gtgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:830786/204‑1 (MQ=255)
                                                                            gtgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGAcc                                                                                                                                                                                                       >  1:371453/1‑216 (MQ=255)
                                                                            gtgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACat                                                                                                                                                                                               <  1:555484/224‑1 (MQ=255)
                                                                            gtgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCg                                                                                                                                                                             <  1:551545/242‑1 (MQ=255)
                                                                            gtgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGt                                                                                                                                                                    >  1:523020/1‑251 (MQ=255)
                                                                             tgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTc                                                                                                                                                                   <  1:114882/251‑1 (MQ=255)
                                                                             tgCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTc                                                                                                                                                                   <  1:809669/251‑1 (MQ=255)
                                                                               cACTTGTTTAATGAAATACTGCAACATTTTTTCGGAGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGa                                                                                                                                                                 >  1:491593/1‑251 (MQ=255)
                                                                                 cTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGtt                                                                                                                                                                                                                             <  1:154286/189‑1 (MQ=255)
                                                                                 cTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTCTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATg                                                                                                                                                               >  1:48483/1‑251 (MQ=255)
                                                                                  ttGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATgg                                                                                                                                                              <  1:710546/251‑1 (MQ=255)
                                                                                   tGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGa                                                                                                                                                             >  1:505912/1‑251 (MQ=255)
                                                                                    gTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTAccc                                                                                                                                                                        >  1:502050/1‑239 (MQ=255)
                                                                                       tAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCCTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATACCCTGACCTTTTACATAAATAAGGGTGTTTGCCGTACCCAGGTCGATGGAAAGa                                                                                                                                                         <  1:442201/251‑1 (MQ=255)
                                                                                        aaTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGAt                                                                                                                                                        >  1:197589/1‑251 (MQ=255)
                                                                                         aTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAATGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGcc                                                                                                                                                                              <  1:716246/228‑1 (MQ=255)
                                                                                         aTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGGCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGAt                                                                                                                                                        >  1:848660/1‑250 (MQ=255)
                                                                                         aTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTAccc                                                                                                                                                                        >  1:483227/1‑234 (MQ=255)
                                                                                         aTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGAt                                                                                                                                                        >  1:28191/1‑250 (MQ=255)
                                                                                         aTGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTGCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTGccc                                                                                                                                                                        <  1:618950/234‑1 (MQ=255)
                                                                                              aTACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTc                                                                                                                                                                   <  1:506649/234‑1 (MQ=255)
                                                                                              aTACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAg                                                                                                                                                  >  1:791008/1‑251 (MQ=255)
                                                                                              aTACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCCACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAg                                                                                                                                                  >  1:54730/1‑251 (MQ=255)
                                                                                                 cTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGc                                                                                                                                                                               <  1:173540/219‑1 (MQ=255)
                                                                                                 cTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGaaa                                                                                                                                               >  1:119260/1‑251 (MQ=255)
                                                                                                    cAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAAc                                                                                                                                             <  1:28600/250‑1 (MQ=255)
                                                                                                    cAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAAc                                                                                                                                             <  1:279932/250‑1 (MQ=255)
                                                                                                         ttttttCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGcc                                                                                                                                                                                                             >  1:378407/1‑181 (MQ=255)
                                                                                                                                  gATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTc                                                                                                                                                                   <  1:164220/198‑1 (MQ=255)
                                                                                                                                   aTAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGaa                                                                                                              <  1:440602/250‑1 (MQ=255)
                                                                                                                                        cccGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGt                                                                                                        <  1:498517/251‑1 (MQ=255)
                                                                                                                                           gTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGAc                                                                                                                                                                                                                   <  1:610718/141‑1 (MQ=255)
                                                                                                                                           gTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTa                                                                                                     >  1:773145/1‑251 (MQ=255)
                                                                                                                                                              gCTTCAATGCTTTTTGGCGTACGACCTAACATTTGTTTCGCTTCTTTACCGACTGCAGCGATAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATACCCTGACCTTTTACATAAATAAGGGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGaa                                                                                   <  1:291766/250‑1 (MQ=255)
                                                                                                                                                               cTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACTGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCgcggcg                                                                                                                                                                                                                                                 <  1:639757/91‑1 (MQ=255)
                                                                                                                                                               cTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATg                                                                                 >  1:577516/1‑251 (MQ=255)
                                                                                                                                                                 tCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGa                                                                                    >  1:532362/1‑246 (MQ=255)
                                                                                                                                                                      gCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAgtg                                                                                                                                                                                      <  1:783061/143‑1 (MQ=255)
                                                                                                                                                                      gCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGaa                                                                                   <  1:273270/242‑1 (MQ=255)
                                                                                                                                                                       cTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGaa                                                                                                              >  1:409569/1‑214 (MQ=255)
                                                                                                                                                                         ttttGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAgtgtgt                                                                                                                                                                                   >  1:91284/1‑143 (MQ=255)
                                                                                                                                                                            tGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGc                                                                    <  1:396875/251‑1 (MQ=255)
                                                                                                                                                                               cGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCtg                                                                                                 >  1:602399/1‑219 (MQ=255)
                                                                                                                                                                                     gCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAAc                                                                                                                                             >  1:821770/1‑169 (MQ=255)
                                                                                                                                                                                     gCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAAc                                                                                                                                             >  1:184749/1‑169 (MQ=255)
                                                                                                                                                                                     gCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGc                                                                    <  1:321673/242‑1 (MQ=255)
                                                                                                                                                                                     gCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑aaaaaa                                                          >  1:223224/1‑251 (MQ=255)
                                                                                                                                                                                       ccAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAAt                                                        <  1:676319/251‑1 (MQ=255)
                                                                                                                                                                                          aCATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGGATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAg                                                                     <  1:720847/236‑1 (MQ=255)
                                                                                                                                                                                          aCATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGaa                                                                                                              >  1:154136/1‑195 (MQ=255)
                                                                                                                                                                                           cATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGc                                                                    <  1:353967/236‑1 (MQ=255)
                                                                                                                                                                                                 tttCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAAt                                              <  1:889493/251‑1 (MQ=255)
                                                                                                                                                                                                        tcttTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGc                                                                    <  1:536348/223‑1 (MQ=255)
                                                                                                                                                                                                        tcttTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGGTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCaa                                       >  1:817137/1‑251 (MQ=255)
                                                                                                                                                                                                          tttACCGACGGCGGCAATGC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGc                                                                    <  1:482595/221‑1 (MQ=255)
                                                                                                                                                                                                                  cggcggCAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAgg                             <  1:479844/251‑1 (MQ=255)
                                                                                                                                                                                                                    gcggcAATAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAggg                            >  1:756901/1‑250 (MQ=255)
                                                                                                                                                                                                                          aTAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAAc                     <  1:743883/251‑1 (MQ=255)
                                                                                                                                                                                                                          aTAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAAc                     <  1:18601/251‑1 (MQ=255)
                                                                                                                                                                                                                          aTAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAAc                     <  1:883198/251‑1 (MQ=255)
                                                                                                                                                                                                                          aTAC‑TTTTCAGAGAACCACTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATACCCTGACCTTTTACATAAATAAGGGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAAc                     <  1:834436/251‑1 (MQ=255)
                                                                                                                                                                                                                           tAC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGTATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTCAAAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAAc                     <  1:865717/251‑1 (MQ=255)
                                                                                                                                                                                                                            aC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACataaata                                                                                                                                                                                          >  1:867903/1‑85 (MQ=255)
                                                                                                                                                                                                                            aC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGaa                                                                                                              >  1:442845/1‑161 (MQ=255)
                                                                                                                                                                                                                            aC‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAAt                                              <  1:416315/224‑1 (MQ=255)
                                                                                                                                                                                                                             c‑TTTTTAGGGAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCg                                                                                                         >  1:85480/1‑165 (MQ=255)
                                                                                                                                                                                                                                     gggAACCGCTGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGaa                                                                                   >  1:347328/1‑180 (MQ=255)
                                                                                                                                                                                                                                              tGCGGTCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAACAGAAAATTTTGTataaata  <  1:419698/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
CGCGACGTTCTACTTGGGTGGCGCCTGCCGGTACGCAAATTAATACGCGTGGGCTCGGACGCATGAAATTGCCGCTGTGCACTTGTTTAATGAAATACTGCAACATTTTTTCGGTGACGAAGAAGTCGGCGATAACCCCGTCTTTCATCGGGCGGATGGCTTCAATGCTTTTTGGCGTACGGCCCAACATTTGTTTCGCTTCTTTACCGACGGCGGCAATAC‑TTTTTAGGGAACCGCTGCGATCGCGGCGAATGGCTACTACGGAAGGTTCGTCAAGGACAATGCCCTGACCTTTTACATAAATAAGTGTGTTTGCCGTACCCAGGTCGATGGAAAGATCGTTAGAAAACAGACCGCGAATTTTTTTAAATAGCATAGGAATTCCGTTTAGCTGAATTGAATAAAGAATGCTCACATTTGAGCGTC‑AAAAAAATTGTACTAAATGTACCAAAAAATGCAGGGTTATAACAGAAAATTTTGTATAAATA  >  2/52671‑53158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑