Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	40,624	T→A	I290N (ATT→AAT)	unknown →	Phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase (EC 2.7.8.20)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	40,624	0	T	A	100.0%	195.6 / NA	72	I290N (ATT→AAT)	unknown	Phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase (EC 2.7.8.20)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (26/46);  total (26/46)

CATTTTCTTCCGACGCCATGGTCAATTCCTTGGTGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAATTATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCACCTTAGCGCAATTA  >  2/40387‑40868                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     cATTTTCTTCCGACGCCATGGTCAATTCCTTGGTGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGattatt                                                                                                                                                                                                                                         >  1:245445/1‑251 (MQ=255)               gCCATGGTCAATTCCTTGGTGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGttt                                                                                                                                                                                                                           >  1:482402/1‑251 (MQ=255)                                 gTGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAg                                                                                                                                                                                                                              <  1:416508/230‑1 (MQ=255)                                  tGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCgg                                                                                                                                                                                                         <  1:437165/250‑1 (MQ=255)                                      gAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCAc                                                                                                                                                                                                      >  1:718576/1‑249 (MQ=255)                                             ggCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCg                                                                                                                                                                                              <  1:880667/250‑1 (MQ=255)                                              gCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCgg                                                                                                                                                                                             <  1:302044/250‑1 (MQ=255)                                              gCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCgg                                                                                                                                                                                             <  1:239728/250‑1 (MQ=255)                                                 aTTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGc                                                                                                                                                                                               >  1:527791/1‑245 (MQ=255)                                                 aTTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCgg                                                                                                                                                                                             <  1:206799/247‑1 (MQ=255)                                                 aTTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTg                                                                                                                                                                                         >  1:537623/1‑251 (MQ=255)                                                     cGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGc                                                                                                                                                                                     <  1:499901/251‑1 (MQ=255)                                                            tATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGcc                                                                                                                                                                              <  1:888941/251‑1 (MQ=255)                                                                          aTTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAAc                                                                                                                                                                <  1:476084/251‑1 (MQ=255)                                                                                     aaGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCgcagca                                                                                                                                                      <  1:826475/250‑1 (MQ=255)                                                                                     aaGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCaa                                                                                                                                                     <  1:216760/251‑1 (MQ=255)                                                                                              cTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGgcgc                                                                                                                                                          >  1:107332/1‑237 (MQ=255)                                                                                              cTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCtt                                                                                                                                            >  1:172038/1‑251 (MQ=255)                                                                                                tCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCtttt                                                                                                                                          >  1:38769/1‑251 (MQ=255)                                                                                                   gAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGgcgc                                                                                                                                                          <  1:142705/232‑1 (MQ=255)                                                                                                              cAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGTCTTTTTGATAATTTATAc                                                                                                                             <  1:54749/250‑1 (MQ=255)                                                                                                                 aaTGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCa                                                                                                                         >  1:810320/1‑251 (MQ=255)                                                                                                                                 aTGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAggc                                                                                                                                                  >  1:152227/1‑210 (MQ=255)                                                                                                                                     tGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTAcgc                                                                                                     <  1:9456/251‑1 (MQ=255)                                                                                                                                      ggACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCTGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTAcgcg                                                                                                    >  1:551142/1‑251 (MQ=255)                                                                                                                                      ggACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTAcgcg                                                                                                    >  1:475444/1‑251 (MQ=255)                                                                                                                                      ggACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTAc                                                                                                       >  1:293536/1‑248 (MQ=255)                                                                                                                                           ttATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAggc                                                                                                                                                  <  1:51641/200‑1 (MQ=255)                                                                                                                                               cAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAg                                                                                           <  1:231540/251‑1 (MQ=255)                                                                                                                                               cAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAg                                                                                           <  1:411128/251‑1 (MQ=255)                                                                                                                                               cAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAg                                                                                           <  1:385225/251‑1 (MQ=255)                                                                                                                                               cAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAg                                                                                           <  1:826635/251‑1 (MQ=255)                                                                                                                                                  gAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGgcgc                                                                                                                                                          >  1:178440/1‑185 (MQ=255)                                                                                                                                                   aaaaGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCg                                                                                         <  1:265600/249‑1 (MQ=255)                                                                                                                                                   aaaaGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATATCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCgg                                                                                        <  1:779283/250‑1 (MQ=255)                                                                                                                                                    aaaGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTg                                                                                      <  1:509270/251‑1 (MQ=255)                                                                                                                                                     aaGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAg                                                                                           >  1:767620/1‑245 (MQ=255)                                                                                                                                                              ccgccCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCt                                                                            <  1:732940/251‑1 (MQ=255)                                                                                                                                                              ccgccCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCt                                                                            <  1:306705/251‑1 (MQ=255)                                                                                                                                                                gccCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCtt                                                                           >  1:459286/1‑250 (MQ=255)                                                                                                                                                                   cGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACg                                                                       <  1:98742/251‑1 (MQ=255)                                                                                                                                                                     gTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTtata                                                                                                                              <  1:159965/194‑1 (MQ=255)                                                                                                                                                                     gTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCgg                                                                                        >  1:405866/1‑232 (MQ=255)                                                                                                                                                                     gTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTc                                                                          <  1:194978/246‑1 (MQ=255)                                                                                                                                                                     gTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGc                                                                      >  1:707637/1‑250 (MQ=255)                                                                                                                                                                      tGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAGTTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     <  1:35468/250‑1 (MQ=255)                                                                                                                                                                      tGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     <  1:458627/250‑1 (MQ=255)                                                                                                                                                                      tGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     <  1:322536/250‑1 (MQ=255)                                                                                                                                                                          ggCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCgcagca                                                                                                                                                      <  1:106981/165‑1 (MQ=255)                                                                                                                                                                          ggCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAaca                                                                 >  1:362989/1‑250 (MQ=255)                                                                                                                                                                           gCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAGGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTTGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACAGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAacac                                                                <  1:238424/250‑1 (MQ=255)                                                                                                                                                                           gCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCAcc                                                                                                                 <  1:331397/201‑1 (MQ=255)                                                                                                                                                                           gCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAacac                                                                <  1:478204/250‑1 (MQ=255)                                                                                                                                                                             ttATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCgg                                                             <  1:594686/251‑1 (MQ=255)                                                                                                                                                                               atatTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGgcg                                                           <  1:660107/251‑1 (MQ=255)                                                                                                                                                                                    tCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGgcgcgcgc                                                      <  1:784388/251‑1 (MQ=255)                                                                                                                                                                                               tCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAAc                                           <  1:811120/251‑1 (MQ=255)                                                                                                                                                                                               tCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAAc                                           <  1:409206/251‑1 (MQ=255)                                                                                                                                                                                                       tGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAAc                                   <  1:688679/251‑1 (MQ=255)                                                                                                                                                                                                               aaTCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCgg                                                                                                                                                                                                         >  1:289972/1‑77 (MQ=255)                                                                                                                                                                                                                aTCAAGCCACTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     >  1:626177/1‑208 (MQ=255)                                                                                                                                                                                                                        aCTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCttttt                  >  1:336504/1‑251 (MQ=255)                                                                                                                                                                                                                        aCTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCttttt                  >  1:153742/1‑251 (MQ=255)                                                                                                                                                                                                                         cTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     <  1:420175/199‑1 (MQ=255)                                                                                                                                                                                                                         cTTATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGcc                                                                     <  1:546489/199‑1 (MQ=255)                                                                                                                                                                                                                           tATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCCCCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGgcgc                                                          <  1:18914/208‑1 (MQ=255)                                                                                                                                                                                                                           tATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGc                                                                                                                           >  1:461743/1‑143 (MQ=255)                                                                                                                                                                                                                           tATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCa                >  1:277235/1‑250 (MQ=255)                                                                                                                                                                                                                           tATCAGGGCAAACCGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCAc               <  1:877210/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCgtggtg                                               <  1:613899/207‑1 (MQ=255)                                                                                                                                                                                                                                       ccGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCACCTTAGCGCAatt   >  1:810080/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGAAAAATATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCACCTTAGCGCAatta  <  1:625323/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CATTTTCTTCCGACGCCATGGTCAATTCCTTGGTGCTGAATTCGGGCTATTCCGTGATTTATGCGGCACAGCAATTTAAAGACGAAGGCACGTCTTCCGAATCTTACGGCAAAATGGAAACGGCGGAAATGCTGGACATTATCAAGAAAAGCGGCAGCCGCCCGGTGGAGGCTTATATTTCCGATGAATTTCCGACGCTGACCCGTAATCAAGCCACTTATCAGGGCAAACCGAAAATTATCGTGATTATTCTGGAAGAAAGTTTCGGCGCGCAATTTATCGGCACCTTAGGCGGTTTGCCGCTTTCGCCGGCATTTGACAAACTGGCGCAGCAAGGCTGGCTTTTTGATAATTTATACGCCACCGGCACCCGCTCCGTACGCGGCATTGAAGCGGTGACCGCAGGCTTCACGCCAACACCGGCGCGCGCCGTGGTGAAACTGAATAACAGCCAAAACGGCTTTTTCACCTTAGCGCAATTA  >  2/40387‑40868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑