Predicted mutation
evidence seq id position mutation annotation gene description
RA 2 27,735 T→C V163A (GTG→GCG)  unknown → Helicase PriA essential for oriC/DnaA‑independent DNA replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*227,7350TC100.0% 279.9 / NA 102V163A (GTG→GCG) unknownHelicase PriA essential for oriC/DnaA‑independent DNA replication
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/50);  total (52/50)

Only 100 of 102 total aligned reads displayed.

GGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGTGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGAACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAGATTTTGCA  >  2/27493‑27975
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
ggcggcGGGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGaaa                                                                                                                                                                                                                                           <  1:596099/250‑1 (MQ=255)
  cggcggGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGcc                                                                                                                                                                                                                                        <  1:305082/251‑1 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAGGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAtt                                                                                                                                                                                                                                 >  1:824956/1‑251 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAtt                                                                                                                                                                                                                                 >  1:758335/1‑251 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAtt                                                                                                                                                                                                                                 >  1:639552/1‑251 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAtt                                                                                                                                                                                                                                 >  1:620695/1‑251 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAt                                                                                                                                                                                                                                  <  1:364491/250‑1 (MQ=255)
         gTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTAGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCACGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGAtt                                                                                                                                                                                                                                 >  1:183110/1‑251 (MQ=255)
             tatCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGt                                                                                                                                                                                                                             <  1:847237/251‑1 (MQ=255)
              atCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTc                                                                                                                                                                                                                            <  1:486199/251‑1 (MQ=255)
                      cccTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAg                                                                                                                                                                                                                     <  1:156707/250‑1 (MQ=255)
                      cccTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGt                                                                                                                                                                                                                    >  1:162404/1‑251 (MQ=255)
                      cccTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGt                                                                                                                                                                                                                    >  1:408928/1‑251 (MQ=255)
                      cccTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGt                                                                                                                                                                                                                    >  1:308108/1‑251 (MQ=255)
                      cccTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGt                                                                                                                                                                                                                    >  1:723767/1‑251 (MQ=255)
                        cTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTg                                                                                                                                                                                                                  >  1:639895/1‑251 (MQ=255)
                                     ttgtCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGa                                                                                                                                                                                                     <  1:351040/251‑1 (MQ=255)
                                      tgtCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGaaa                                                                                                                                                                                                                                           <  1:633587/212‑1 (MQ=255)
                                           cAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCTTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATcc                                                                                                                                                                                                <  1:875423/250‑1 (MQ=255)
                                               cGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAAcc                                                                                                                                                                                           <  1:239804/251‑1 (MQ=255)
                                                       gTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAGCCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGgcagc                                                                                                                                                                                    <  1:421682/250‑1 (MQ=255)
                                                                     gggCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATa                                                                                                                                                                     >  1:422024/1‑251 (MQ=255)
                                                                     gggCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATa                                                                                                                                                                     >  1:677576/1‑251 (MQ=255)
                                                                                  gTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGaa                                                                                                                                                        >  1:183771/1‑251 (MQ=255)
                                                                                   tGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAAt                                                                                                                                                       <  1:775757/251‑1 (MQ=255)
                                                                                   tGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAAt                                                                                                                                                       <  1:841284/251‑1 (MQ=255)
                                                                                      aaaaCCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATaa                                                                                                                                                     <  1:802010/250‑1 (MQ=255)
                                                                                       aaaCCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGaa                                                                                                                                                                                                          <  1:567347/196‑1 (MQ=255)
                                                                                        aaCCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGc                                                                                                                                                  <  1:134437/251‑1 (MQ=255)
                                                                                        aaCCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGc                                                                                                                                                  <  1:502516/251‑1 (MQ=255)
                                                                                               gtgtTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGt                                                                                                                                           >  1:308497/1‑251 (MQ=255)
                                                                                                      ttGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAAc                                                                                                                                      >  1:74044/1‑249 (MQ=255)
                                                                                                      ttGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCt                                                                                                                                    <  1:665833/251‑1 (MQ=255)
                                                                                                           aaCCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               <  1:208341/251‑1 (MQ=255)
                                                                                                           aaCCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               <  1:312033/251‑1 (MQ=255)
                                                                                                           aaCCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               <  1:343204/251‑1 (MQ=255)
                                                                                                             ccgaccgCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACaa                                                                                                                             <  1:516550/251‑1 (MQ=255)
                                                                                                                    cACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCg                                                                                                                                                                >  1:318048/1‑209 (MQ=255)
                                                                                                                            gcgCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgt                                                                                                              >  1:829870/3‑251 (MQ=255)
                                                                                                                             aGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgt                                                                                                              <  1:472348/250‑1 (MQ=255)
                                                                                                                               cAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgtgt                                                                                                            <  1:23373/250‑1 (MQ=255)
                                                                                                                               cAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgtgt                                                                                                            <  1:74228/250‑1 (MQ=255)
                                                                                                                               cAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgtgt                                                                                                            <  1:421457/250‑1 (MQ=255)
                                                                                                                                 ggCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               >  1:506570/1‑229 (MQ=255)
                                                                                                                                 ggCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTtca                                                                                                         >  1:152509/1‑251 (MQ=255)
                                                                                                                                  gCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAAt                                                                                                                                                       >  1:552564/1‑204 (MQ=255)
                                                                                                                                     ttGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTtcagt                                                                                                       >  1:831466/1‑249 (MQ=255)
                                                                                                                                       ggCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATc                                                                                                                                             <  1:196121/209‑1 (MQ=255)
                                                                                                                                       ggCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATc                                                                                                                                             <  1:710148/209‑1 (MQ=255)
                                                                                                                                       ggCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTcagtcagt                                                                                                   >  1:422658/1‑251 (MQ=255)
                                                                                                                                        gcgcAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGtt                                                                                                  >  1:842249/1‑251 (MQ=255)
                                                                                                                                        gcgcAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGtt                                                                                                  >  1:306638/1‑251 (MQ=255)
                                                                                                                                           cAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGa                                                                                                <  1:137855/250‑1 (MQ=255)
                                                                                                                                             gggCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAt                                                                                             >  1:145354/1‑251 (MQ=255)
                                                                                                                                             gggCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAt                                                                                             >  1:637199/1‑251 (MQ=255)
                                                                                                                                              ggCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAtt                                                                                            >  1:725226/1‑251 (MQ=255)
                                                                                                                                              ggCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAtt                                                                                            >  1:570973/1‑251 (MQ=255)
                                                                                                                                               gCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCt                                                                                                                                    <  1:62533/210‑1 (MQ=255)
                                                                                                                                               gCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAgtg                                                                                                             <  1:125981/233‑1 (MQ=255)
                                                                                                                                               gCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAttt                                                                                           >  1:799472/1‑251 (MQ=255)
                                                                                                                                               gCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAAttt                                                                                           >  1:204642/1‑251 (MQ=255)
                                                                                                                                                   aTTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCTCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAAc                                                                                                                                      <  1:392904/204‑1 (MQ=255)
                                                                                                                                                   aTTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGcaac                                                                                                                                                                                 <  1:207199/161‑1 (MQ=255)
                                                                                                                                                   aTTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTc                                                                                       <  1:205437/251‑1 (MQ=255)
                                                                                                                                                         aCGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGtt                                                                                 <  1:832988/251‑1 (MQ=255)
                                                                                                                                                          cgcgcgAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGttt                                                                                >  1:342444/1‑251 (MQ=255)
                                                                                                                                                              cgAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGGCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGt                                                                             <  1:432300/250‑1 (MQ=255)
                                                                                                                                                                aagaagCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTcc                                                                           <  1:263508/250‑1 (MQ=255)
                                                                                                                                                                aagaagCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTcc                                                                           <  1:142398/250‑1 (MQ=255)
                                                                                                                                                                       agcTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               >  1:759621/1‑191 (MQ=255)
                                                                                                                                                                        gcTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAAc                                                                                                                               <  1:280671/190‑1 (MQ=255)
                                                                                                                                                                             aaGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGttgttggac                                                             <  1:47234/251‑4 (MQ=255)
                                                                                                                                                                             aaGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGttgttggac                                                             <  1:211558/251‑4 (MQ=255)
                                                                                                                                                                               gCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGttgttggac                                                             >  1:554057/1‑246 (MQ=255)
                                                                                                                                                                                cGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACgg                                                           <  1:11319/250‑1 (MQ=255)
                                                                                                                                                                                       ggcggcGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACgg                                                   >  1:669309/1‑251 (MQ=255)
                                                                                                                                                                                        gcggcgAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACgg                                                   <  1:218001/250‑1 (MQ=255)
                                                                                                                                                                                        gcggcgAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACgg                                                   <  1:877915/250‑1 (MQ=255)
                                                                                                                                                                                                    ttGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAAc                                      <  1:339656/251‑1 (MQ=255)
                                                                                                                                                                                                          gagaAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAAt                                >  1:59485/1‑251 (MQ=255)
                                                                                                                                                                                                                 gCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGttgttggac                                                             <  1:564657/215‑4 (MQ=255)
                                                                                                                                                                                                                 gCAACAACCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTg                         >  1:76718/1‑251 (MQ=255)
                                                                                                                                                                                                                  caacaaCCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGttgttggac                                                             >  1:503555/1‑211 (MQ=255)
                                                                                                                                                                                                                    acaacCCTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCaa                      <  1:355925/251‑1 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGc                                                                                                                                                  >  1:668951/1‑123 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:256571/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:514967/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:177991/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:838103/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:440786/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTa                  >  1:744204/1‑251 (MQ=255)
                                                                                                                                                                                                                          cTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCt                                                                                                                                    >  1:845226/1‑135 (MQ=255)
                                                                                                                                                                                                                          cTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATc                               <  1:289529/236‑1 (MQ=255)
                                                                                                                                                                                                                          cTTTTAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTAt                 >  1:604068/1‑250 (MQ=255)
                                                                                                                                                                                                                              tAGTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAag            <  1:637928/251‑1 (MQ=255)
                                                                                                                                                                                                                                gTGCGGCGATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAagag          >  1:166552/1‑251 (MQ=255)
                                                                                                                                                                                                                                     gcgATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAGAtttt     >  1:683637/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTg                                                                       >  1:842130/1‑183 (MQ=255)
                                                                                                                                                                                                                                       gATTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAGATTTTg    >  1:179022/1‑250 (MQ=255)
                                                                                                                                                                                                                                        aTTTGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAGATTTTgca  <  1:760969/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
GGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGAAAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAACGCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATTTGGTCGGTGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGGCGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTACTCAGGGTTTTGTCCCGTGGTTGTTGAACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAGATTTTGCA  >  2/27493‑27975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑