| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 2 | 27,288 | A→G | H14R (CAC→CGC) | unknown → | Helicase PriA essential for oriC/DnaA‑independent DNA replication |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 2 | 27,288 | 0 | A | G | 100.0% | 183.5 / NA | 67 | H14R (CAC→CGC) | unknown | Helicase PriA essential for oriC/DnaA‑independent DNA replication |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/39); total (28/39) | |||||||||||
AATATAAATTTTCATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCACTATTTTGATTATATCTTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGT > 2/27043‑27530 | aaTATAAATTTTCATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTAtt < 1:220226/251‑1 (MQ=255) atatAAATTTTCATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTAttt > 1:434292/1‑251 (MQ=255) aaTTTTCATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGAtt < 1:717303/251‑1 (MQ=255) cATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATcc < 1:728888/251‑2 (MQ=255) cttcttAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGAtt > 1:591633/1‑237 (MQ=255) ttcattcaTCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑ggg < 1:22450/251‑1 (MQ=255) ccTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCTCTGAATGCTGTCGT‑GGGGGCGAGGgt > 1:804732/1‑251 (MQ=255) gTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGtgtg > 1:416800/1‑251 (MQ=255) tcttAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGgcg > 1:382399/1‑251 (MQ=255) gTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATcc < 1:657843/181‑2 (MQ=255) aTTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGAt > 1:609174/1‑172 (MQ=255) aTTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGc < 1:396367/198‑1 (MQ=255) aTTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCa > 1:489436/1‑251 (MQ=255) tATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCAtggt < 1:169291/251‑1 (MQ=255) cccTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAggg < 1:4908/208‑1 (MQ=255) cccTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCAtggtgg > 1:841895/1‑250 (MQ=255) cccTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATggtggt > 1:106555/1‑251 (MQ=255) tATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAg < 1:786387/251‑1 (MQ=255) aTCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGCCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAgg > 1:44698/1‑251 (MQ=255) aTCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATACTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATcc < 1:696008/167‑2 (MQ=255) aTTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAggg < 1:698429/192‑1 (MQ=255) aCCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAg < 1:23511/213‑1 (MQ=255) cccAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGAtg < 1:723524/251‑1 (MQ=255) acaGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGC‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGGGGCGGAGCAGTCGGa > 1:381330/1‑241 (MQ=255) ggagCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGt < 1:303116/223‑1 (MQ=255) gagCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAgg < 1:329096/222‑1 (MQ=255) gagCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAg > 1:614531/1‑251 (MQ=255) gagCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAg > 1:510697/1‑251 (MQ=255) aTTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGAtgt > 1:889957/1‑236 (MQ=255) ttCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTg < 1:667678/251‑1 (MQ=255) tcCCCCAAATAGACAACAAGCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAg < 1:494790/251‑1 (MQ=255) cccccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑gg < 1:330281/155‑1 (MQ=255) ccccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGAc < 1:879609/251‑1 (MQ=255) ccccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGAc < 1:131291/251‑1 (MQ=255) ccccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGAc < 1:393547/251‑1 (MQ=255) ccccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGAc < 1:222231/251‑1 (MQ=255) cccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGt < 1:392846/148‑1 (MQ=255) cccAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTACCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACa > 1:437130/1‑251 (MQ=255) cAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGt < 1:375051/172‑1 (MQ=255) cAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTgggg < 1:303591/178‑1 (MQ=255) aaTAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCa > 1:530946/1‑194 (MQ=255) aGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGAtgtg > 1:806557/1‑223 (MQ=255) acaacaATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCt < 1:473478/251‑1 (MQ=255) acaacaATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCt < 1:195368/251‑1 (MQ=255) acaacaATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCt < 1:24239/251‑1 (MQ=255) aTGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGa < 1:865053/201‑1 (MQ=255) gCTATATTCCGAGCCCATTTTATTTCACTGAATTTCTGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGCGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAg > 1:537142/1‑182 (MQ=255) gCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGc < 1:405886/170‑1 (MQ=255) gCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGt > 1:503640/1‑184 (MQ=255) gCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGTGGTTTc > 1:196446/1‑250 (MQ=255) gCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTc > 1:384687/1‑250 (MQ=255) atTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCt < 1:151769/228‑1 (MQ=255) cccATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAgg > 1:283435/1‑233 (MQ=255) ccATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCa > 1:250455/1‑251 (MQ=255) ttATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGTGGGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCtt < 1:285116/235‑1 (MQ=255) cTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGAc < 1:726495/196‑1 (MQ=255) ttCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCt < 1:565964/195‑1 (MQ=255) ttCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTggg > 1:265747/1‑250 (MQ=255) cccGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGtattat < 1:311922/251‑1 (MQ=255) cGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCa < 1:245259/251‑1 (MQ=255) cGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCa < 1:892435/251‑1 (MQ=255) cGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCa < 1:780210/251‑1 (MQ=255) ttCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTg > 1:471265/1‑251 (MQ=255) tCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATtgat > 1:361616/1‑178 (MQ=255) tCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTgg > 1:599089/1‑251 (MQ=255) tgtgCCGTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTggggg < 1:867413/251‑1 (MQ=255) gTTACATCGCTATTTTGATTATATCCTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAgtgt > 1:531304/1‑251 (MQ=255) | AATATAAATTTTCATATTTGCTTCTTAGCTAAGATGCTTTTTCATTCATCCTGTACGCATTTCTTGCTTCTTAAATTTGACAGTATTTTATCCCTTATCCAAGTGAAATTAAACCCCAAACAACAGAGCTATTCTCCCCCAAATAGACAACAATCCCCCAAAATGCTATATTCCGAGCCCATTTTATTTCACTGAATTTCCGTTCATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCACTATTTTGATTATATCTTGCCGGCGTCGCTGAATGCTGTCGT‑GGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATGTGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTGCAGTGGGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGT > 2/27043‑27530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |