Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	327,084	T→C	V251A (GTA→GCA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	327,084	0	T	C	100.0%	135.8 / NA	48	V251A (GTA→GCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/21);  total (27/21)

CTTGTTGTTAAGTGAAATTTTTATTCGCCAATTACACGATCAAAACTGGCGCATTCTGCCTAAAATTCTTACCTTTTTAGTGTTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGTAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGACTGCATTAATTTGGCTTTTTTTAACG  >  1/326839‑327325                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  cTTGTTGTTAAGTGAAATTTTTATTCGCCAATTACACGATCAAAACTGGCGCATTCTGCCTAAAATTCTTACCTTTTTAGTGTTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTa                                                                                                                                                                                                                                              <  1:135562/251‑1 (MQ=255)             tGAAATTTTTATTCGCCAATTACACGATCAAAACTGGCGCATTCTGCCTAAAATTCTTACCTTTTTAGTGTTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCg                                                                                                                                                                                                                                  <  1:487863/251‑1 (MQ=255)                                       aTCAAAACTGGCGCATTCTGCCTAAAATTCTTACCTTTTTAGTGTTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGtattttcctattttc                                                                                                                                                                                                         >  1:595287/1‑250 (MQ=255)                                                                                gtgtTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCg                                                                                                                                                                                                                                  <  1:797154/184‑1 (MQ=255)                                                                                         cATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTc                                                                                                                                                                                                                                       >  1:807163/1‑170 (MQ=255)                                                                                                   gCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGt                                                                                                                                                                                                                       >  1:844303/1‑176 (MQ=255)                                                                                                   gCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGAt                                                                                                                                             >  1:665914/1‑250 (MQ=255)                                                                                                         gtgtTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATc                                                                                                                                            >  1:197367/1‑245 (MQ=255)                                                                                                          tgtTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATg                                                                                                                                                                                                    >  1:46950/1‑188 (MQ=255)                                                                                                                taCCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTAtgt                                                                                                                               <  1:92615/251‑1 (MQ=255)                                                                                                                      tAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTAtgt                                                                                                                               >  1:493925/1‑245 (MQ=255)                                                                                                                          ttACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCAtgg                                                                                                                     <  1:101718/251‑1 (MQ=255)                                                                                                                                  cGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGtata                                                                                                             <  1:180244/251‑1 (MQ=255)                                                                                                                                    atatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTAtgt                                                                                                                               <  1:374232/231‑1 (MQ=255)                                                                                                                                    atatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTCACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGtattttcc                                                                                                                                                                                                                <  1:791095/150‑1 (MQ=255)                                                                                                                                     tatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagc                                                                                                          <  1:734025/251‑1 (MQ=255)                                                                                                                                     tatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAag                                                                                                           >  1:37699/1‑250 (MQ=255)                                                                                                                                     tatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAag                                                                                                           >  1:271979/1‑250 (MQ=255)                                                                                                                                     tatTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGCGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACt                                                                                                                                                                                                                                               >  1:169265/1‑118 (MQ=255)                                                                                                                                        ttACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagcag                                                                                                        >  1:788395/1‑250 (MQ=255)                                                                                                                                        ttACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagcag                                                                                                        >  1:489673/1‑250 (MQ=255)                                                                                                                                          aCTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCAt                                                                                                     >  1:825957/1‑251 (MQ=255)                                                                                                                                               ggTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGa                                                                                                                                              >  1:839341/1‑205 (MQ=255)                                                                                                                                                gtgtgtATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATcc                                                                                                <  1:445492/250‑1 (MQ=255)                                                                                                                                                  gtgtATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCAtgg                                                                                                                     >  1:604237/1‑227 (MQ=255)                                                                                                                                                  gtgtATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCa                                                                                             >  1:452824/1‑251 (MQ=255)                                                                                                                                                           tttCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGaa                                                                                    <  1:584950/251‑1 (MQ=255)                                                                                                                                                                  tgtTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTc                                                                              <  1:622922/250‑1 (MQ=255)                                                                                                                                                                   gtTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagc                                                                                                          >  1:496389/1‑221 (MQ=255)                                                                                                                                                                             gtggtgATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATcc                                                                                                >  1:759918/1‑221 (MQ=255)                                                                                                                                                                             gtggtgATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCa                                                                       >  1:442374/1‑246 (MQ=255)                                                                                                                                                                              tggtgATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTAtgt                                                                                                                               <  1:556216/189‑1 (MQ=255)                                                                                                                                                                                 tgATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCAtt                                                              <  1:33350/251‑1 (MQ=255)                                                                                                                                                                                    ttCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCAttttt                                                           >  1:720766/1‑250 (MQ=255)                                                                                                                                                                                      ccATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagc                                                                                                          >  1:476878/1‑202 (MQ=255)                                                                                                                                                                                            gggATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAAc                                                                                   <  1:652984/219‑1 (MQ=255)                                                                                                                                                                                                    gATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATc                                                                                                                                            >  1:383714/1‑154 (MQ=255)                                                                                                                                                                                                             gtATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTa                                  >  1:521998/1‑251 (MQ=255)                                                                                                                                                                                                             gtATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTa                                  >  1:366338/1‑251 (MQ=255)                                                                                                                                                                                                                taAAAAATACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAagc                                                                                                          >  1:89553/1‑176 (MQ=255)                                                                                                                                                                                                                    aaaTACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGAc                           <  1:549586/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGAc                           <  1:127895/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTACCGATTTGATTTTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGAc                           <  1:196691/251‑1 (MQ=255)                                                                                                                                                                                                                                gatttTGTTAATTTAATTACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTAtgt                                                                                                                               <  1:467645/139‑1 (MQ=255)                                                                                                                                                                                                                                         aatttaattACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTttat                                                                                                                                                      >  1:78239/1‑107 (MQ=255)                                                                                                                                                                                                                                             taattACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGACTGCATTAATTTGGCTTTTTTTAACg  <  1:852648/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tACTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGAc                           >  1:456211/1‑222 (MQ=255)                                                                                                                                                                                                                                                  aCTGCAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAgg                                                                                                                                                                     <  1:141379/83‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CTTGTTGTTAAGTGAAATTTTTATTCGCCAATTACACGATCAAAACTGGCGCATTCTGCCTAAAATTCTTACCTTTTTAGTGTTAAGTCATTTATTCGGCGTCCGTGTTATTACCGATAATTTACCTAACGATATTTACTCCGGTGTGTATTTTTTTCTTGTGTTTTTACAAGTGGTGATTCCATCGGGGATCGTGATTTATGTGTATAAAAAATACCGATTTGATTTTGTTAATTTAATTACTGTAACTATCTGTTCTATCGTTGCTTTTGGTATTTTCCTATTTTCCAATGTAAATTCCGGTGATTTTGCCGTATTGGCAGGCCTGTTGCTTTTTATTATTACGATCTTTGCCATTATGTTGTTGCATGGTTGGTATAAGCAGCATTATCCGCACCTTAAGAACATTTCTTGGGCACTGTCCATTTTATTAATTTTTGCCATTTTTATCGGTATTGTGACTGCATTAATTTGGCTTTTTTTAACG  >  1/326839‑327325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑