Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	21,757	A→C	F92C (TTT→TGT)	unknown ←	COG0859: ADP‑heptose:LPS heptosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	21,757	0	A	C	100.0%	174.0 / NA	62	F92C (TTT→TGT)	unknown	COG0859: ADP‑heptose:LPS heptosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/26);  total (36/26)

GAAATATGTGTTGTGTCATTTTGTGGACAAGCGAAATTATAATTTTTGATTGTATCTAAATTATAATTTTCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGAAATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAACAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATCATTATTAAATCTATGTTTTCTG  >  2/21574‑21982                                                                                                                                                                                        |                                                                                                                                                                                                                                  gAAATATGTGTTGTGTCATTTTGTGGACAAGCGAAATTATAATTTTTGATTGTATCTAAATTATAATTTTCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAAt                                                                                                                                                                >  1:706535/1‑251 (MQ=255)                 cATTTTGTGGACAAGCGAAATTATAATTTTTGATTGTATCTAAATTATAATTTTCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAg                                                                                                                                                                                                                     <  1:71352/182‑1 (MQ=255)                                                          aaaTTATAATTTTCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAgg                                                                                                                                                                                                <  1:612818/162‑1 (MQ=255)                                                                     ttCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTa                                                                                            >  1:315929/1‑251 (MQ=255)                                                                      tCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAAc                                                                                                                                                                                     <  1:385111/161‑1 (MQ=255)                                                                      tCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGt                                                                                                                 >  1:348225/1‑229 (MQ=255)                                                                                  ttattaAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGa                                                                                                                                            <  1:478780/190‑1 (MQ=255)                                                                                      taAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           <  1:272487/251‑1 (MQ=255)                                                                                         acacAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACg                                                                        >  1:523605/1‑251 (MQ=255)                                                                                          cacaATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGc                                                                            >  1:321041/1‑246 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCaaaaa                                                                                                                                                                 >  1:748129/1‑159 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCTGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:316172/1‑251 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           <  1:223396/245‑1 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           >  1:98150/1‑245 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGc                                                                      <  1:832704/250‑1 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:285367/1‑251 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:794023/1‑251 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:674030/1‑251 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:809085/1‑251 (MQ=255)                                                                                            caATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCa                                                                     >  1:139509/1‑251 (MQ=255)                                                                                              ataatGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTa                                                                                                                                                                                   <  1:436779/139‑1 (MQ=255)                                                                                              ataatGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTc                                                                                                                                                 <  1:782631/173‑1 (MQ=255)                                                                                              ataatGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGa                                                                                                                                            >  1:114245/1‑178 (MQ=255)                                                                                              ataatGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAg                                                                                                                         >  1:152386/1‑197 (MQ=255)                                                                                              ataatGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCAt                                                                    >  1:431505/1‑250 (MQ=255)                                                                                                          ggTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACAt                                                                                                                                                                                         <  1:86486/121‑1 (MQ=255)                                                                                                          ggTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATc                                                                                                                                       >  1:170398/1‑171 (MQ=255)                                                                                                          ggTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           <  1:615070/231‑1 (MQ=255)                                                                                                          ggTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCAt                                                        >  1:365335/1‑250 (MQ=255)                                                                                                               tAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCtg                                                                                                                                               >  1:634590/1‑158 (MQ=255)                                                                                                                       tctcTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATc                                                                                                                                                                               >  1:848050/1‑118 (MQ=255)                                                                                                                       tctcTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGc                                                                      >  1:602193/1‑223 (MQ=255)                                                                                                                        ctctAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGgag                                                                                                                                                                                              <  1:617123/102‑1 (MQ=255)                                                                                                                        ctctAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGa                                                                                                                                            <  1:326073/152‑1 (MQ=255)                                                                                                                        ctctAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGt                                                                                                                 <  1:139544/179‑1 (MQ=255)                                                                                                                          ctAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTaac                                       <  1:521494/251‑1 (MQ=255)                                                                                                                          ctAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTaac                                       <  1:698019/251‑1 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTaac                                       >  1:65215/1‑246 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAacaaca                                   >  1:817334/1‑250 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAacaaca                                   >  1:386660/1‑250 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAacaaca                                   >  1:628373/1‑250 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGAGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAacaaca                                   >  1:447972/1‑250 (MQ=255)                                                                                                                               ataataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAACAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGc                                                                            <  1:446849/209‑1 (MQ=255)                                                                                                                                taataGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATgg                                               >  1:225061/1‑237 (MQ=255)                                                                                                                                     gACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATc                                                                                                                                                                               <  1:539391/104‑1 (MQ=255)                                                                                                                                     gACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAAt                                                                                                                               <  1:512776/152‑1 (MQ=255)                                                                                                                                     gACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           <  1:370333/204‑1 (MQ=255)                                                                                                                                     gACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGAttt                            >  1:188097/1‑251 (MQ=255)                                                                                                                                      aCTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAAt                                                                                                   >  1:590107/1‑179 (MQ=255)                                                                                                                                      aCTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTa                           >  1:259842/1‑251 (MQ=255)                                                                                                                                         tAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATc                        <  1:606248/251‑1 (MQ=255)                                                                                                                                              gtgtAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACt                                                                                                                                                                                    <  1:80932/90‑1 (MQ=255)                                                                                                                                              gtgtAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATc                        <  1:644418/246‑1 (MQ=255)                                                                                                                                               tgtAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATCattatt                  <  1:247579/251‑1 (MQ=255)                                                                                                                                                                tCCAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATCATTATTAAATCTATGTTTTCTg  >  1:342685/1‑250 (MQ=255)                                                                                                                                                                  cAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCa                                                                           >  1:100481/1‑175 (MQ=255)                                                                                                                                                                  cAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGAttt                            >  1:373682/1‑222 (MQ=255)                                                                                                                                                                  cAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATc                        >  1:816108/1‑226 (MQ=255)                                                                                                                                                                  cAAATAGAGATCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATc                        <  1:514074/226‑1 (MQ=255)                                                                                                                                                                          gaTCCCATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGa                                                                                                                                            >  1:75732/1‑102 (MQ=255)                                                                                                                                                                              ccATTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATCATTATTAAATc             >  1:681153/1‑225 (MQ=255)                                                                                                                                                                                aTTTGCGACATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAATAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGAtt                             <  1:720417/207‑1 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                                                  GAAATATGTGTTGTGTCATTTTGTGGACAAGCGAAATTATAATTTTTGATTGTATCTAAATTATAATTTTCCTTATGTTTTTTATTAAACACAATAATGTACTTCGGTGATAACAGCTTCTCTAAAATAATAGACTTAGTGGTGTAAGTTGGCTGAAAATCCAAATAGAGATCCCATTTGCGAAATTGAGAAATATAGGTTAAAGGTTTGTCCTCCAGGAGAACATCAACTAAATCGGAACAAGCAAAAATGTCCCGATTCCTTTCTGTGACGATCACGCCAATCACCAGTTCCGGGTTGGCAAGTTTTAATTGTTTTAAATGCGCGGTATGGGCAACGGCATCGCCAATAGCATCGCCAATGGGTTTTAACAACACCGATTTAATCATTATTAAATCTATGTTTTCTG  >  2/21574‑21982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑