Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	2	19,002	T→C	G70G (GGT→GGC)	unknown →	UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	2	19,002	0	T	C	96.3%	199.1 / ‑5.2	82	G70G (GGT→GGC)	unknown	UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (46/32);  total (46/36)
Fisher's exact test for biased strand distribution p-value = 7.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGAATCTTTTGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGTGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTACC  >  2/18762‑19237                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             cGAATCTTTTGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAg                                                                                                                                                                                                                                     <  1:419472/250‑1 (MQ=255)   aaTCTTTTGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGttt                                                                                                                                                                                                                                  >  1:643875/1‑251 (MQ=255)     tCTTTTGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTAt                                                                                                                                                                                                                                >  1:868942/1‑251 (MQ=255)       ttttGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTc                                                                                                                                                                                                                              <  1:91975/251‑1 (MQ=255)            tttttAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAg                                                                                                                                                                                                                            >  1:880425/1‑248 (MQ=255)                             cAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         <  1:235736/250‑1 (MQ=255)                               aatgaatgAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGa                                                                                                                                                                                                      >  1:477358/1‑251 (MQ=255)                                 tgaatgaaGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:572349/1‑246 (MQ=255)                                 tgaatgaaGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑cc                                                                                                                                                                                                   <  1:742476/251‑1 (MQ=255)                                 tgaatgaaGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑cc                                                                                                                                                                                                   <  1:158734/251‑1 (MQ=255)                                  gaatgaaGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:393218/1‑245 (MQ=255)                                  gaatgaaGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTACCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:434734/1‑245 (MQ=255)                                         gCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTc                                                                                                                                                                                                                              <  1:156771/217‑1 (MQ=255)                                         gCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTg                                                                                                                                                                                                            <  1:826085/235‑1 (MQ=255)                                         gCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTa                                                                                                                                                                                           >  1:820898/1‑251 (MQ=255)                                          cATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGc                                                                                                                                                                                                                                       <  1:65598/207‑1 (MQ=255)                                                     aGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGa                                                                                                                                                                               >  1:765603/1‑251 (MQ=255)                                                            aGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTaaaa                                                                                                                                                                        >  1:853965/1‑251 (MQ=255)                                                             ttttttAAAATGGGGTGCTGCCGTTTGGGCGGACGATGAATTTTGGGCTTTTTTTTTGCGTAGGGAAATTAAGGACTGCCGGGTGATGCATTTATCAAAAAATGTATTGAAACTTTTTGG‑TTCTTTCCGGGATTTATTTCGTCCCGACA‑AAAA‑GC‑GTTTTGGCGTGGTGAAAGGCTTTGGCGTTACGCATTTTATTCAGCGGAAAGCTTGTACTAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAag                                                                                                                                                                       <  1:208375/247‑1 (MQ=255)                                                             tctataaaactgcGGTGCTCCCGTTTGGCCGGACGATGACTTTTTGGCGTTTTTTTTGCGTAGGGAAATTAAGGCCTGCCCGGTGATGCATTTATCAAAAAATGTATTGCAACTTTTTGG‑TTTTTTACGCGATTTATTTCGTCCCGACA‑AAAA‑GCATTTTT‑GCGTTGTAAAAGGCATCGGCTTTCCGCTTTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAag                                                                                                                                                                       <  1:388425/239‑1 (MQ=255)                                                             tcTATTAAAATGGGGTGCTGCCGTTTTGACGGACTATGAACTATTGGCGATCTTTTTGGGTACGGAAATTAAGGACTCCCCGGTGATGCATTTATCAAAAAATTTATTGCAACTTTTTGG‑TTCTTTACGCGATTTATTTCGTGCCGACC‑AAAA‑GGATTTTT‑GCGTTGTGAAGGGCTTTGGCTTTACGCTTTTTATTCAGCGGCAAGCCTGTACTGAAAGGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAag                                                                                                                                                                       <  1:815513/250‑1 (MQ=255)                                                             tcTATAAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTTTTGGCGTTTTTTTTGCGTAGGGAAATAAAGGCCGGCCGGGTGATGCGTTTATAAAAAAATGTATTGCAACATTTTGG‑TTTTTTACGCGAGTTATTTCGTGCCGACA‑AAAA‑GCATTTTT‑GCGCTGTGAAAGGCTTGGGCGTTACGCATTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAag                                                                                                                                                                       <  1:428577/250‑1 (MQ=255)                                                             gCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCa                                                                                                                                                                                                                             >  1:667399/1‑198 (MQ=255)                                                             gCTATTAAAATACGGTGCAGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:254602/1‑218 (MQ=255)                                                                aTTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGcc                                                                                                                                                                                                                   >  1:219044/1‑205 (MQ=255)                                                                aTTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑cc                                                                                                                                                                                                   <  1:668236/220‑1 (MQ=255)                                                                aTTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑cc                                                                                                                                                                                                   <  1:131043/220‑1 (MQ=255)                                                                 ttAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGc                                                                                                                                                                                                                                       <  1:848038/184‑1 (MQ=255)                                                                   aaaaTACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:186176/1‑212 (MQ=255)                                                                   aaaaTACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGAcc                                                                                                                                                                  >  1:502239/1‑250 (MQ=255)                                                                   aaaaTACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGAcc                                                                                                                                                                  >  1:135606/1‑250 (MQ=255)                                                                     aaTACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCa                                                                                                                                                                >  1:459254/1‑250 (MQ=255)                                                                      aTACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCa                                                                                                                                                                >  1:463847/1‑249 (MQ=255)                                                                           gTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTAtt                                                                                                                                                                                                                               >  1:750142/1‑182 (MQ=255)                                                                           gTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGcc                                                                                                                                                                                                                   <  1:224624/194‑1 (MQ=255)                                                                           gTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         <  1:404217/204‑1 (MQ=255)                                                                                   gCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGcc                                                                                                                                                                                                                   <  1:6658/186‑1 (MQ=255)                                                                                    ttttGGCGGACGATGAATTATTGGCGATTTTTTTGCGTACGGAAATTAAGGACTGCCGGTTAATGCATTTATCGAAAAATGTTTTGCAAC‑TTTTGGTTTTTTTACGCAATTTATTTGGTGCGGACA‑AAAA‑GCATTTTG‑GCGCTGTAAAGGGCATTGGCTTTACGCAGTTTATTCAGCTGAAAGCCTGTACTAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAAcc                                                                                                                                                <  1:802271/250‑1 (MQ=255)                                                                                                   aaCTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATg                                                                                                                                 >  1:819693/1‑251 (MQ=255)                                                                                                    aCTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGAGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGCGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGt                                                                                                                                >  1:136735/1‑251 (MQ=255)                                                                                                           gcgttttttttGCGTGGGGGAATTAAGGACTGCCGGGTGATGCAGTTATCAAAAAATGTATTGAAACTTTTTGT‑TTTTTTACGGGAGTTAATTCGTGCCGCCA‑AAAA‑GCAGTTTT‑GCGCTGTAAAAGGCTTTGGCTTTCCGCATTTTATTCAGCTGCAAGCCTGTACTAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTAc                                                                                                                          <  1:359468/244‑1 (MQ=255)                                                                                                              aTCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTAc                                                                                                                                                                                                              >  1:490721/1‑164 (MQ=255)                                                                                                                cTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         <  1:453949/167‑1 (MQ=255)                                                                                                                   tttGCGTAGGGGAATAAAGGACGCCCCGGTGATGAAGTTACAAAAAAATGTATGGAAACTTTTTGT‑TTCTTTAGGGGATTTAATTCGTGCC‑ACCAAAAA‑GAATTTTT‑GCGTTGTAAAAGGCTTTGGCGTTACGTATTTTATTCAGCGACAACCCTTTACTAAAATGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGc                                                                                                                 <  1:62575/251‑1 (MQ=255)                                                                                                                             ggAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAatat                                                                                                       >  1:68051/1‑251 (MQ=255)                                                                                                                              gAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGttt                                                                                                                                                                                                                                  >  1:469868/1‑128 (MQ=255)                                                                                                                              gAATAAAGGTCTGCCCGGTGATGCATTTATCAAAAAATGTATTGCAACTTTTTGT‑TTCTTTACGCGATTTAATTCGTGCCGACA‑AAAA‑GCAGTTTT‑GCTCTGTGAAAGCCTTTGGCTTTCCGCATTTTATTCAGCTGCAAGCTTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAatat                                                                                                       <  1:769381/250‑1 (MQ=255)                                                                                                                                aTTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         <  1:141102/151‑1 (MQ=255)                                                                                                                                aTTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGaaa                                                                                                                                                                                                         >  1:661130/1‑151 (MQ=255)                                                                                                                                aTTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAag                                                                                                                                                                       >  1:422423/1‑184 (MQ=255)                                                                                                                                          gCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTGAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAAtt                                                                                          >  1:441945/1‑251 (MQ=255)                                                                                                                                          gCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAAtt                                                                                          >  1:482387/1‑251 (MQ=255)                                                                                                                                               gTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGCGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTAt                                                                                     >  1:448328/1‑251 (MQ=255)                                                                                                                                                   tGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTg                                                                                 >  1:145573/1‑251 (MQ=255)                                                                                                                                                       gTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCttt                                                                             >  1:204736/1‑251 (MQ=255)                                                                                                                                                          ataaaaaattgttttgaatctttttGT‑TTTTTTGCGCGTGTTATTTCGTGCC‑ACC‑AAAAGGCATTTTT‑GCGCTGGGAAGGGCTTTGGCTTTACGCATTTTATTCGGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTc                                                                          <  1:855487/230‑1 (MQ=255)                                                                                                                                                          aTCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATgg                                                                                   >  1:51300/1‑242 (MQ=255)                                                                                                                                                             gAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTgg                                                                       >  1:794241/1‑251 (MQ=255)                                                                                                                                                                          cAACTTTTTGT‑TTTTTTACGAGATTTAATTCGTGCCGACA‑AAAA‑GCAGTTTG‑GCGCTGTGAAGGGCTTTGGCGTTACGCAGTTTATTCAGCGGAAAGCTTTTACGAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATc                                                           <  1:295943/250‑1 (MQ=255)                                                                                                                                                                            aCATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGc                                                         >  1:299084/1‑250 (MQ=255)                                                                                                                                                                              aTTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGc                                                                                                                 >  1:158867/1‑192 (MQ=255)                                                                                                                                                                               ttttGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGa                                                     >  1:502845/1‑251 (MQ=255)                                                                                                                                                                                tttGT‑TTCTTTCCGCGTGTTATTTCGTGCCGCCC‑AAAA‑GCATTTTT‑GCGCTGTAAAAGGCTTTGGCTTTACGCATTTTATTCAGCTGCAAGCCTTTACTAAAATGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGAt                                                    <  1:255718/251‑1 (MQ=255)                                                                                                                                                                                   tgtttCTTTACGGGATTTAATTCGTGCCGAAA‑AAAA‑GCATTTTT‑GCGCTGTGAAAGGCTTTGGCGTTACGAAGTTTTTTCAGCGGAAAGCCTGTACTAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTa                                                  <  1:350702/248‑1 (MQ=255)                                                                                                                                                                                      ttttttccgggAGTTAATTCGGGCCGACA‑AAAA‑GCTTTTTG‑GCGCGGTAAAGGGCATTGGCGTTCCGCGTTTTATCCAGCTGCAACCCTGTACTGAAATGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGc                                               <  1:611/241‑1 (MQ=255)                                                                                                                                                                                      ttctttgcggaaGTTATTTCGTGCCGACA‑AAAA‑GCTGTTTT‑GCATTGGAAAAGGCTTTGACGTTCCGAATTTTATTCAGCTGAAAGCTTTTACT‑AAAAGACCCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGc                                               <  1:354160/240‑1 (MQ=255)                                                                                                                                                                                      ttCTTTACGCGATTTATTTCGGGCCGACA‑AAAA‑GCAGTTTT‑GCGTTGTGAAGGGCGTTGGCGTTACGCGTTTTATTAAGCTGCAAGCCTGTACTAAAATGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGc                                               <  1:779627/251‑1 (MQ=255)                                                                                                                                                                                        cTTTACGGGAGTTATTTCGTGCCGACA‑AAAA‑GCATTTTT‑GCGCTGTGAAAGGCATTGGGGTTCCGCATTTTATTCAGCTGCAAGCTTGTACTAAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTa                                                  <  1:498287/246‑1 (MQ=255)                                                                                                                                                                                           aaaagaatttAATCCGGGCCGACA‑AAAA‑GCATTTTT‑GCGCTGGGAAGGGGTTTGGTTTTACGGATTTTATTCAGCGGAAGGCCTATACTAAAATGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGaa                                          <  1:81037/243‑1 (MQ=255)                                                                                                                                                                                                     aaTTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGttt                                 >  1:131051/1‑250 (MQ=255)                                                                                                                                                                                                                aCC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCAtt                     >  1:734147/1‑251 (MQ=255)                                                                                                                                                                                                                aCC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCAtt                     >  1:189396/1‑251 (MQ=255)                                                                                                                                                                                                                     aaa‑GCAGTTTT‑CCGCTGTAAAAGGCATTGTCTTTACGCAGTTTATTCAGCGGCAAGCTTGTGGTAAAATCC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATtaata                 <  1:882438/251‑1 (MQ=255)                                                                                                                                                                                                                          ctgTTTG‑GCGCTGTAAAGGGCTTGGGTTTTACGTATTTTATTCAGCTGCAAGCTTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAg              <  1:544097/248‑1 (MQ=255)                                                                                                                                                                                                                          cAGTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAg              >  1:9799/1‑250 (MQ=255)                                                                                                                                                                                                                           attttttgcgttggaaaaGGCTTGGGTGTTACGTATTTTTTCCAGCTGAAGGCCTGTACAGAAGTGC‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAg              <  1:242414/235‑1 (MQ=255)                                                                                                                                                                                                                            gTTTT‑GCGCTGTGAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCa                                                                                                            >  1:55637/1‑154 (MQ=255)                                                                                                                                                                                                                                       gtgAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAg                                                                                                                        >  1:437187/1‑132 (MQ=255)                                                                                                                                                                                                                                       gtgAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTAcc  >  1:844132/1‑250 (MQ=255)                                                                                                                                                                                                                                       gtgAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTAcc  >  1:446021/1‑250 (MQ=255)                                                                                                                                                                                                                                       gtgAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTAcc  >  1:900966/1‑250 (MQ=255)                                                                                                                                                                                                                                       gtgAAAGGCATTGGCGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTAcc  >  1:122829/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             CGAATCTTTTGTTTTTAGAGAGGTTCGGCAAATGAATGAAGCATTAATGCCAAGGGAAAAGCTATTAAAATACGGTGCTGCCGCTTTGACGGACGATGAACTATTGGCGATCTTTTTGCGTACGGGAATTAAGGACTGCCCGGTGATGCAGTTATCGAAAAATGTATTGCAACATTTTGG‑TTCTTTACGCGAGTTAATTCGTGCCGACC‑AAAA‑GCAGTTTT‑GCGCTGTGAAAGGCATTGGTGTTACGCAGTTTATTCAGCTGCAAGCCTGTACTGAAATGA‑CCCGCCGTTATTTGTTTGCAGAATTAAAAGAGACCCACTGTTTTACTTCAACCGACATTGTGAAAATGTACTTACAGACGGAGCTGGCAAATATCGAGCGGGAAATTTTTATGGTGCTTTTTCTGGATAACCAACATCGCCTGATTAAGCAGGAACGCCTGTTTCTCGGCACCATTAATAAAGCGATGGTGTACC  >  2/18762‑19237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑