Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 326,152 T→G S432A (TCC→GCC)  unknown → Glycerol‑3‑phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1326,1520TG100.0% 143.7 / NA 52S432A (TCC→GCC) unknownGlycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/31);  total (21/31)

TATCTGTTTGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTTCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAATCTTCTCTGTTTAATTTATCTTGGCCACGTTATTTA  >  1/325911‑326380
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
tatCTGTTTGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACtt                                                                                                                                                                                                                             >  1:100345/1‑251 (MQ=255)
     gTTTGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCg                                                                                                                                                                                                                                   <  1:853647/240‑1 (MQ=255)
      tttGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTTGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTc                                                                                                                                                                                                                        <  1:863113/250‑1 (MQ=255)
      tttGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTcc                                                                                                                                                                                                                       <  1:441688/251‑1 (MQ=255)
      tttGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTc                                                                                                                                                                                                                        <  1:398536/250‑1 (MQ=255)
              cAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAAcc                                                                                                                                                                                                                <  1:566721/250‑1 (MQ=255)
              cAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCg                                                                                                                                                                                                               >  1:248466/1‑251 (MQ=255)
                    gTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATAc                                                                                                                                                                                                          <  1:369241/250‑1 (MQ=255)
                                          gatgatgatCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAAcc                                                                                                                                                                                                                <  1:770477/222‑1 (MQ=255)
                                                     gCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTc                                                                                                                                                                                                                        <  1:358154/203‑1 (MQ=255)
                                                      cTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAg                                                                                                                                                                       >  1:516520/1‑251 (MQ=255)
                                                                gcagATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGTATACCAAGAAATAATCTTTATTCTTAaataat                                                                                                                                                                              <  1:546989/234‑1 (MQ=255)
                                                                gcagATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAg                                                                                                                                                                       <  1:427749/241‑1 (MQ=255)
                                                                   gATTTGGTTCCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGaa                                                                                                                                                                                                             <  1:423519/200‑1 (MQ=255)
                                                                   gATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaataa                                                                                                                                                                               <  1:228143/230‑1 (MQ=255)
                                                                    aTTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAAtt                                                                                                                                                          <  1:216840/250‑1 (MQ=255)
                                                                             cTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAaat                                                                                                                                                                                                  >  1:822350/1‑201 (MQ=255)
                                                                                gtgtGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAAt                                                                                                                                              >  1:873370/1‑250 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTc                                                                                                                                                                                                                                 >  1:491857/1‑155 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTc                                                                                                                                                                                                                        <  1:67850/164‑1 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAACCCg                                                                                                                                                                                                               >  1:439086/1‑173 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaat                                                                                                                                                                                 >  1:406988/1‑203 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaat                                                                                                                                                                                 >  1:216768/1‑203 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAg                                                                                                                                                                       >  1:27407/1‑213 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCAc                                                                                                                                     <  1:753960/247‑1 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACtttt                                                                                                                                 >  1:322083/1‑251 (MQ=255)
                                                                                            gTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACtttt                                                                                                                                 >  1:785425/1‑251 (MQ=255)
                                                                                             tACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGAGGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACttttt                                                                                                                                <  1:432578/251‑1 (MQ=255)
                                                                                                 gCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaat                                                                                                                                                                                 <  1:481143/198‑1 (MQ=255)
                                                                                                      aGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaat                                                                                                                                                                                 <  1:580555/193‑1 (MQ=255)
                                                                                                                                tgttgGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAAc                                                                                                                                                                                                                 <  1:678806/135‑1 (MQ=255)
                                                                                                                                tgttgGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGc                                                                                               >  1:455579/1‑249 (MQ=255)
                                                                                                                                     ggATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGAtt                                                                                        >  1:669140/1‑251 (MQ=255)
                                                                                                                                                       tATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATaa                                                                      <  1:453137/251‑1 (MQ=255)
                                                                                                                                                         tgtgATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAAt                                                                    <  1:326197/251‑1 (MQ=255)
                                                                                                                                                          gtgATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAAtt                                                                   >  1:289017/1‑251 (MQ=255)
                                                                                                                                                               gggTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCAc                                                                                                                                          <  1:545588/175‑1 (MQ=255)
                                                                                                                                                                      cTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCAttt                                                                                                                       >  1:735375/1‑187 (MQ=255)
                                                                                                                                                                      cTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAg                                                        >  1:20893/1‑250 (MQ=255)
                                                                                                                                                                             aTGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCaaaaaa                                                 <  1:414339/250‑1 (MQ=255)
                                                                                                                                                                               gTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAaataa                                                                                                                                                                               >  1:520178/1‑122 (MQ=255)
                                                                                                                                                                                    cGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAaat                                         >  1:8219/1‑251 (MQ=255)
                                                                                                                                                                                     ggTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATAc                                                                                                                                                                                                          >  1:845869/1‑89 (MQ=255)
                                                                                                                                                                                     ggTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATc                                                                                                                                                                                              <  1:869914/101‑1 (MQ=255)
                                                                                                                                                                                           gACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAAtct                                   <  1:482133/250‑1 (MQ=255)
                                                                                                                                                                                                        aTGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAATCTTCTCTGTTTAAttt                     <  1:635463/251‑1 (MQ=255)
                                                                                                                                                                                                               cATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAATCTTCTCTGttt                          >  1:288138/1‑239 (MQ=255)
                                                                                                                                                                                                                 tCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATc                                                                <  1:73070/199‑1 (MQ=255)
                                                                                                                                                                                                                  ccTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTc                                                                                                                                                                                                                        <  1:607801/46‑1 (MQ=255)
                                                                                                                                                                                                                  ccTTTCTTGTTTCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAg                                                                                                     <  1:583998/161‑1 (MQ=255)
                                                                                                                                                                                                                           tttCCTCGGTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAATCTTCTCTGTTTAATTTATCTTGGCCACGTTATTTa  <  1:94583/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gTTTTGCTTTTATTGCCGTCACTTTATTCCATAAACCGAATAc                                                                                                                                                                                                          <  1:690156/43‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
TATCTGTTTGGTCGCAATTGGTTTCCTGATTTACGGACCGGTGATGATGATCGGCTTACAGGCAGCAGATTTGGTACCTCGTGTGGCGACCGGTACAGCAACAGGCTTAACCGGTTTATTTGGTTACTTGTTGGGATCTGCCAGTGCCGGTTATGTGATGGGTAAACTGGTGGATGTATTCGGTTGGGACGGCGGATTTTATGCGCTCATCCTTTCTTGTTTCCTCGGTTTTGCTTTTATTTCCGTCACTTTATTCCATAAACCGAATACCAAGAAATAATCTTTATTCTTAAATAATACTAAAGTGCGGTTGAAATTCGCGGCGAAAATCCACCGCACTTTTTCTTTCATTTTTCTGTTGAAGTTGGCAGAATAGCCTCGATTTTTTACGCATAATTATAAATTATCAAGGAAAGCAAAAAATGATAAATCAATCTTCTCTGTTTAATTTATCTTGGCCACGTTATTTA  >  1/325911‑326380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑