Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	320,390	T→C	L153L (CTA→CTG)	unknown ←	Histidine permease YuiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	320,390	0	T	C	100.0%	200.0 / NA	71	L153L (CTA→CTG)	unknown	Histidine permease YuiF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/32);  total (39/32)

TTGTCGGCTCGGTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTTAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAGTAAATCGGTAAT  >  1/320146‑320617                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    ttGTCGGCTCGGTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGcac                                                                                                                                                                                                                                <  1:670382/250‑1 (MQ=255) ttGTCGGCTCGGTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCATACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGcac                                                                                                                                                                                                                                <  1:721145/250‑1 (MQ=255)     cGGCTCGGTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAg                                                                                                                                                                                                                           <  1:735889/251‑1 (MQ=255)            gTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCg                                                                                                                                                                                                                     >  1:233771/1‑250 (MQ=255)            gTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCg                                                                                                                                                                                                                     >  1:507307/1‑250 (MQ=255)            gTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGCTAACAACCCCAAAACCACGCCGATCACGGGAAAGGTCATTGCCAGAGACACTTTGCCCACGCTGGTTTGTAAGCCTAACGGACTGCCTACGGTGCTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTACGAAACCCACCGGCAATAACATATAGGTGGCGGCTAAACCGAAGGTCAGGACACAAACCACCg                                                                                                                                                                                                                     >  1:688136/1‑250 (MQ=255)             tGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGc                                                                                                                                                                                                                    <  1:446339/250‑1 (MQ=255)                 catcaCCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCAcggc                                                                                                                                                                                                               <  1:783552/251‑1 (MQ=255)                   tcaCCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCAcggcgg                                                                                                                                                                                                             >  1:680619/1‑251 (MQ=255)                   tcaCCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCAcggcgg                                                                                                                                                                                                             >  1:551292/1‑251 (MQ=255)                      ccacGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGc                                                                                                                                                                                                                    <  1:507510/241‑1 (MQ=255)                             ttCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCAAGCCGAACACGGGAATGGTCATTGCCAGTGACACTTCGCCCCCGCTGGTTTGTAAGCCTAATGAATTGCCTACGGCGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGCTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGtcaattttca                                                                                                                                                                                                   >  1:99082/1‑251 (MQ=255)                                     gTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGtcaattttcaatt                                                                                                                                                                                                >  1:171477/1‑246 (MQ=255)                                     gTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATttgttg                                                                                                                                                                                           >  1:16252/1‑251 (MQ=255)                                                gTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAAt                                                                                                                                                                                      <  1:439851/245‑1 (MQ=255)                                                gTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAg                                                                                                                                                                                >  1:195802/1‑251 (MQ=255)                                                gTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAg                                                                                                                                                                                >  1:164091/1‑251 (MQ=255)                                                       acacGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATcgg                                                                                                                                                                          >  1:532047/1‑250 (MQ=255)                                                                            aaaCCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAg                                                                                                                                                    >  1:508680/1‑251 (MQ=255)                                                                              aCCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAgg                                                                                                                                                   >  1:591112/1‑250 (MQ=255)                                                                              aCCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGc                                                                                                                                                  <  1:843187/251‑1 (MQ=255)                                                                                 aTGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAAt                                                                                                                                               >  1:71077/1‑251 (MQ=255)                                                                                        tCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTAc                                                                                                                                         >  1:548913/1‑250 (MQ=255)                                                                                          aCGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCgg                                                                                                                                      <  1:753833/251‑1 (MQ=255)                                                                                                   gTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGtt                                                                                                                             >  1:36554/1‑251 (MQ=255)                                                                                                          ccAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGga                                                                                                                       <  1:475272/250‑1 (MQ=255)                                                                                                             gTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAgg                                                                                                                                                   <  1:678659/219‑1 (MQ=255)                                                                                                                 cacTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGc                                                                                                                                                  >  1:121114/1‑216 (MQ=255)                                                                                                                     tCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAgg                                                                                                                                                           >  1:587617/1‑203 (MQ=255)                                                                                                                      cGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAg                                                                                                               >  1:351762/1‑246 (MQ=255)                                                                                                                            cGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGcc                                                                                                                                                                                                                         >  1:386608/1‑134 (MQ=255)                                                                                                                               tGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAgg                                                                                                 <  1:187735/251‑1 (MQ=255)                                                                                                                                ggTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGc                                                                                                                                                  >  1:835739/1‑201 (MQ=255)                                                                                                                                ggTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGa                                                                                                >  1:694764/1‑251 (MQ=255)                                                                                                                                ggTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGa                                                                                                >  1:764285/1‑251 (MQ=255)                                                                                                                                   ttGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGc                                                                                                                                                  >  1:728884/1‑198 (MQ=255)                                                                                                                                   ttGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATAc                                                                                             <  1:702654/251‑1 (MQ=255)                                                                                                                                      tAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCg                                                                                           <  1:614703/250‑1 (MQ=255)                                                                                                                                          ccTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGc                                                                                        <  1:7981/249‑1 (MQ=255)                                                                                                                                                  aTTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATAtt                                                                              <  1:651434/251‑1 (MQ=255)                                                                                                                                                    tGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACTGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGa                                                                                                <  1:424762/231‑1 (MQ=255)                                                                                                                                                     gCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGa                                                                                                                                                               <  1:272343/167‑1 (MQ=255)                                                                                                                                                            gtgtTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGgc                                                                                                                                     >  1:78891/1‑186 (MQ=255)                                                                                                                                                            gtgtTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAAt                                                                                                                 >  1:365399/1‑206 (MQ=255)                                                                                                                                                            gtgtTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGa                                                                                                            >  1:79664/1‑211 (MQ=255)                                                                                                                                                            gtgtTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGc                                                                                        <  1:394026/231‑1 (MQ=255)                                                                                                                                                              gtTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTg                                                                            >  1:478936/1‑241 (MQ=255)                                                                                                                                                                  atatTTTTCAACAGAATTCTTTCGATAAAGATTTTTCCGAAACACACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGc                                                               <  1:821959/250‑1 (MQ=255)                                                                                                                                                                     tttttCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGtt                                                           >  1:47733/1‑251 (MQ=255)                                                                                                                                                                          cACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCgg                                                      <  1:364983/251‑1 (MQ=255)                                                                                                                                                                               gAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCGCACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGc                                                               >  1:357404/1‑237 (MQ=255)                                                                                                                                                                                       tCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTAtt                                         <  1:380472/251‑1 (MQ=255)                                                                                                                                                                                        cGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTg                                                         >  1:681911/1‑234 (MQ=255)                                                                                                                                                                                           tAAAGATTTTTCCGAGACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTGGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATg                                     <  1:814889/251‑1 (MQ=255)                                                                                                                                                                                           tAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATg                                     <  1:430256/251‑1 (MQ=255)                                                                                                                                                                                              aGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATAtt                                                                              >  1:604158/1‑207 (MQ=255)                                                                                                                                                                                              aGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCgg                                  >  1:295276/1‑251 (MQ=255)                                                                                                                                                                                                aTTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCggg                                 <  1:544313/250‑1 (MQ=255)                                                                                                                                                                                                                   gCAATAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGc                                                               >  1:554824/1‑201 (MQ=255)                                                                                                                                                                                                                      aTAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGc                                                                                                                                                  <  1:725836/115‑1 (MQ=255)                                                                                                                                                                                                                      aTAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTgatgat                          <  1:243429/235‑1 (MQ=255)                                                                                                                                                                                                                       tAACATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAGTAAAt         <  1:422179/251‑1 (MQ=255)                                                                                                                                                                                                                         aCATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATGCCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAg              <  1:31157/244‑1 (MQ=255)                                                                                                                                                                                                                         aCATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACAt                                                                       >  1:811605/1‑187 (MQ=255)                                                                                                                                                                                                                         aCATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAGTAAATCg       >  1:887205/1‑251 (MQ=255)                                                                                                                                                                                                                         aCATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTA‑TCATGCGGGTGATGGTTTTGTAGGCAAGTAAATCgg      >  1:451307/1‑251 (MQ=255)                                                                                                                                                                                                                          cATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGc                                                               >  1:405988/1‑194 (MQ=255)                                                                                                                                                                                                                          cATATAGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGc                                    >  1:762118/1‑221 (MQ=255)                                                                                                                                                                                                                              taGGTGGCGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAGTAAATCGGTAAt  <  1:571884/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGGTTAAACCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAgg                  <  1:692725/228‑1 (MQ=255)                                                                                                                                                                                                                                           aaCCGAAGGTCAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGc                                                        <  1:664920/184‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    TTGTCGGCTCGGTGCTCATCACCACGTATTCGCGCGGTTTGCGATAAGTGATAAACACGGCGGTTAACAACCCCAAAACCATGCCGATCACGGGAATGGTCATTGCCAGTGACACTTCGCCCACGCTGGTTTGTAAGCCTAATGAATTGCCTACGGTGTTGATATTTTTCACCAGAATGCTTTCGATAAAGATTTTTCCGAAACCCACCGGCAATAACATATAGGTGGCGGTTAAACCGAAGGTTAGCACACAAGCCACCGCACGGCGGTCAATTTTCAATTTGTTGAAAATGGCGAGCATCGGCGGAATGACGATAGGAATAAAGGCAATGTGTACCGGCAGCAGGTTTTGGGAAGAAATAGCGAACAGCGTTAGGATACCGAGCATGGCATATTTGAACATGGCAAGGCTGTTTGCGGTTGGCACTTTATTCATGCGGGTGATGATTTTGTAGGCAAGTAAATCGGTAAT  >  1/320146‑320617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑