Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,695,359	G→A	S219S (TCC→TCT)	unknown ←	Glucose‑6‑phosphate 1‑dehydrogenase (EC 1.1.1.49)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,695,359	0	G	A	100.0%	227.2 / NA	80	S219S (TCC→TCT)	unknown	Glucose‑6‑phosphate 1‑dehydrogenase (EC 1.1.1.49)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/31);  total (49/31)

CGCCGTCCACTTCGCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAGCGGGCGCAAACAATGTAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCGCCGGCGGCTCCATCGCCACCATGGCAAGCACTTGCAATAAGTGGTTTTGGAACATATCGCGCATGGCACCGGAACCGTCATAATAGCCGCCGCGCTCTTCCACGCCAATGGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCAAAGCGGTTCAAA‑CCAGCCGTTGGAGAAGCGCAATACGAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAGCGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTTGCGGTCATAACCGAACGGTTTTTCCACGATAATAC  >  1/1695121‑1695582                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 cGCCGTCCACTTCGCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCg                                                                                                                                                                                                                      >  1:237081/1‑251 (MQ=255)  gCCGTCCACTTCGCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCgg                                                                                                                                                                                                                     >  1:176008/1‑251 (MQ=255)      tCCACTTCGCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGAt                                                                                                                                                                                                                 >  1:209583/1‑251 (MQ=255)              gCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGt                                                                                                                                                                                                         >  1:385250/1‑251 (MQ=255)                 tGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTACTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGAATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAAt                                                                                                                                                                                                      <  1:605839/251‑1 (MQ=255)                  gCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAAt                                                                                                                                                                                                      >  1:570861/1‑250 (MQ=255)                   cGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATc                                                                                                                                                                                                     <  1:840587/250‑1 (MQ=255)                     aCATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAAt                                                                                                                                                                                                  <  1:601868/251‑1 (MQ=255)                            gTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCTACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGaaa                                                                                                                                                                                              <  1:25615/248‑1 (MQ=255)                               ccAACACCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACgg                                                                                                                                                                                        <  1:819222/251‑1 (MQ=255)                                   cacCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTc                                                                                                                                                                                     >  1:788018/1‑250 (MQ=255)                                    acCAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGt                                                                                                                                                                                                         <  1:787573/229‑1 (MQ=255)                                     ccAGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTcc                                                                                                                                                                                    <  1:357670/249‑1 (MQ=255)                                       aGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAAt                                                                                                                                                                                             <  1:893091/238‑1 (MQ=255)                                       aGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                >  1:678768/1‑248 (MQ=255)                                       aGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                >  1:780644/1‑248 (MQ=255)                                       aGATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                >  1:868470/1‑248 (MQ=255)                                        gATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAAt                                                                                                                                                                                             >  1:898080/1‑237 (MQ=255)                                        gATTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                >  1:774698/1‑247 (MQ=255)                                         aTTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                <  1:572456/249‑4 (MQ=255)                                         aTTATGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGGTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                <  1:439940/249‑4 (MQ=255)                                            aTGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaac                                                                                                                                                                               <  1:314608/247‑5 (MQ=255)                                             tGCTCCACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaacggctc                                                                                                                                                                          <  1:623417/251‑10 (MQ=255)                                                  cACGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑cc                                                                                                                                                                    <  1:729660/251‑1 (MQ=255)                                                   aCGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCa                                                                                                                                                                   >  1:263344/1‑251 (MQ=255)                                                    cGTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCaa                                                                                                                                                                  >  1:116550/1‑250 (MQ=255)                                                     gTCTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCaa                                                                                                                                                                  >  1:764360/1‑249 (MQ=255)                                                       cTTCCTGGGTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCg                                                                                                                                                               >  1:30297/1‑251 (MQ=255)                                                              ggTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTGCACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGgaaa                                                                                                                                                        >  1:443443/1‑249 (MQ=255)                                                               gTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGgaaaa                                                                                                                                                       >  1:335017/1‑248 (MQ=255)                                                               gTCAACGGGCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGgaaa                                                                                                                                                        <  1:605068/250‑3 (MQ=255)                                                                      ggCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                <  1:311244/220‑4 (MQ=255)                                                                      ggCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCaa                                                                                                                                                 >  1:787430/1‑250 (MQ=255)                                                                      ggCGCAAACAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCaa                                                                                                                                                 >  1:485259/1‑250 (MQ=255)                                                                           aaaCAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCaacacaag                                                                                                                                           >  1:779049/1‑245 (MQ=255)                                                                           aaaCAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCaacacaag                                                                                                                                           <  1:198346/251‑7 (MQ=255)                                                                              cAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAg                                                                                                                                        >  1:724349/1‑251 (MQ=255)                                                                              cAATGCAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAg                                                                                                                                        >  1:463977/1‑251 (MQ=255)                                                                                atgcaGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGt                                                                                                                                      >  1:806350/5‑251 (MQ=255)                                                                                       aCTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGa                                                                                                                                >  1:256544/1‑250 (MQ=255)                                                                                            cGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGttt                                                                                                                          >  1:792286/1‑251 (MQ=255)                                                                                                  ttCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTAc                                                                                                                    <  1:202902/251‑1 (MQ=255)                                                                                                   tCGTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTAcc                                                                                                                   <  1:612461/251‑1 (MQ=255)                                                                                                     gTCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaa                                                                                                                                                                                >  1:834690/1‑186 (MQ=255)                                                                                                      tCACGCATGGAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAg                                                                                                                <  1:89783/251‑1 (MQ=255)                                                                                                               gAATTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGga                                                                                                                                                          >  1:37023/1‑200 (MQ=255)                                                                                                                aaTTGGCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCg                                                                                                      >  1:809899/1‑251 (MQ=255)                                                                                                                     gCGTTGATAATCTCCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCGTCGCGCCGGGACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATAc                                                                                                  <  1:211925/250‑1 (MQ=255)                                                                                                                     gCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAGCATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATAc                                                                                                  <  1:194740/250‑1 (MQ=255)                                                                                                                     gCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATAc                                                                                                  <  1:437226/250‑1 (MQ=255)                                                                                                                     gCGTTGATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACg                                                                                                 >  1:629919/1‑251 (MQ=255)                                                                                                                          gATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTAc                                                                                                                    >  1:528597/1‑227 (MQ=255)                                                                                                                          gATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTaaa                                                                                            >  1:548167/1‑251 (MQ=255)                                                                                                                          gATAATCACCGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTaaa                                                                                            >  1:240138/1‑251 (MQ=255)                                                                                                                              atcaccGGCGGCTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTg                                                                                        >  1:180786/5‑251 (MQ=255)                                                                                                                                      cggcTCCATCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGttctt                                                                                >  1:112384/1‑251 (MQ=255)                                                                                                                                              tCGCCACCATTGCCAGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAACCCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTAc                                                                                                                    >  1:601863/1‑208 (MQ=255)                                                                                                                                                       ttgccaGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTaa                                                                                             <  1:657273/216‑1 (MQ=255)                                                                                                                                                        tgccaGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACCCAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAAttt                                                              >  1:448466/5‑251 (MQ=255)                                                                                                                                                        tgccaGCACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAAttt                                                              >  1:336284/5‑251 (MQ=255)                                                                                                                                                              cACTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAatat                                                        <  1:205394/251‑1 (MQ=255)                                                                                                                                                                cTTGTAATAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATc                                                       >  1:258809/1‑250 (MQ=255)                                                                                                                                                                   gtaaTAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCaac                                                                                                                                                                 >  1:266038/3‑139 (MQ=255)                                                                                                                                                                   gtaaTAAATGGTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAaaga                                                                          >  1:800911/3‑228 (MQ=255)                                                                                                                                                                             gTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCacaac                                                                                                                                                                               >  1:640870/1‑114 (MQ=255)                                                                                                                                                                             gTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTcggcgg                                         >  1:128263/1‑251 (MQ=255)                                                                                                                                                                             gTTTTGGAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTcggcgg                                         >  1:867695/1‑251 (MQ=255)                                                                                                                                                                                  ggAACATATCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGtttca                                    >  1:545137/1‑249 (MQ=255)                                                                                                                                                                                       atatCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATc                                                                                                                                                                                                     <  1:216362/86‑1 (MQ=255)                                                                                                                                                                                       atatCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTAcc                                                                                                                   >  1:337107/1‑167 (MQ=255)                                                                                                                                                                                       atatCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATg                                                                                     <  1:687001/197‑1 (MQ=255)                                                                                                                                                                                       atatCGCGCATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAatat                                                        <  1:652432/226‑1 (MQ=255)                                                                                                                                                                                         atcgcgcatcgcgcCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCAt                             >  1:552059/1‑251 (MQ=255)                                                                                                                                                                                             cgcATCGCGCCGGAACCGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAAcc                         <  1:695239/251‑1 (MQ=255)                                                                                                                                                                                                            ccGTCATACTAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCAc          <  1:399879/251‑1 (MQ=255)                                                                                                                                                                                                            ccGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCAc          <  1:567534/251‑1 (MQ=255)                                                                                                                                                                                                             cGTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCAca         >  1:122547/1‑250 (MQ=255)                                                                                                                                                                                                              gTCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCAcaa        >  1:692766/1‑249 (MQ=255)                                                                                                                                                                                                               tCATAATAGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGCTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCAcaat       >  1:876575/1‑248 (MQ=255)                                                                                                                                                                                                                    ataGCCGCCACGCTCTTCTACACCGATAGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCACAACGGTTCAAA‑CCAACCGTTGGAAAAGCGCAACACAAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAACGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTCACGGTCATAACCGAACGGTTTTTCCACAataagac  <  1:451319/251‑4 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 CGCCGTCCACTTCGCCTGCGACATATTGTCCCAACACCAGATTATGCTCCACGTCTTCCTGGGTCAGCGGGCGCAAACAATGTAGCACTTTCGCCACTTCGTCACGCATGGAATTGGCGTTGATAATCGCCGGCGGCTCCATCGCCACCATGGCAAGCACTTGCAATAAGTGGTTTTGGAACATATCGCGCATGGCACCGGAACCGTCATAATAGCCGCCGCGCTCTTCCACGCCAATGGATTCCGCGCCGGTGATTTCAATGTAATCAATGAAATTACGGTTCCAAAGCGGTTCAAA‑CCAGCCGTTGGAGAAGCGCAATACGAGCAGGTTTTGGACGGTTTCTTTACCAAGATAATGGTCGATACGGTAAATTTGATGTTCTTCAAAGAAGCGGTGAATTTGAATATCCAGCGCTTCGGCGGTTTTGCGGTCATAACCGAACGGTTTTTCCACGATAATAC  >  1/1695121‑1695582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑