Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	313,145	C→T	G137S (GGC→AGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	313,145	0	C	T	100.0%	212.0 / NA	78	G137S (GGC→AGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/43);  total (35/43)

CTGAAAGGAATTTAACGCGCTCACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCCGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGTTTTACGTAATCC  >  1/312906‑313391                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       cTGAAAGGAATTTAACGCGCTCACCTGCTAAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTaaaa                                                                                                                                                                                                                                             <  1:269218/251‑1 (MQ=255)        gAATTTAACGCGCTCACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGTGTTGATTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCaa                                                                                                                                                                                                                                      >  1:185879/1‑251 (MQ=255)                    cTCACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTg                                                                                                                                                                                                                          >  1:536901/1‑251 (MQ=255)                      cACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGAttttt                                                                                                                                                                                                                            <  1:611780/247‑1 (MQ=255)                      cACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGAt                                                                                                                                                                                                                        >  1:885342/1‑251 (MQ=255)                      cACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGAt                                                                                                                                                                                                                        >  1:667746/1‑251 (MQ=255)                      cACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGAt                                                                                                                                                                                                                        >  1:723204/1‑251 (MQ=255)                            ctgAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGc                                                                                                                                                                                                                   >  1:253467/1‑250 (MQ=255)                                  atTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAATTAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGAtg                                                                                                                                                                                                             <  1:458838/251‑1 (MQ=255)                                       cAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTc                                                                                                                                                                                                        <  1:600999/250‑1 (MQ=255)                                                 aaaaaTGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTaaacacaa                                                                                                                                                                                             >  1:308907/1‑251 (MQ=255)                                                              cAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACaa                                                                                                                                                                                 <  1:721678/250‑1 (MQ=255)                                                                 ccGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAg                                                                                                                                                                             <  1:274313/251‑1 (MQ=255)                                                                  cGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGGCCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGc                                                                                                                                                                            <  1:444065/251‑1 (MQ=255)                                                                  cGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAg                                                                                                                                                                             >  1:525131/1‑250 (MQ=255)                                                                  cGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGc                                                                                                                                                                            <  1:866435/251‑1 (MQ=255)                                                                    aCATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCa                                                                                                                                                                           <  1:391386/250‑1 (MQ=255)                                                                            tATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACg                                                                                                                                                                  <  1:150795/251‑1 (MQ=255)                                                                                     aaTTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACg                                                                                                                                                         >  1:517420/1‑251 (MQ=255)                                                                                      aTTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGAt                                                                                                                                                                       >  1:62497/1‑236 (MQ=255)                                                                                               gAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATg                                                                                                                                                                                                                       <  1:495372/179‑1 (MQ=255)                                                                                                aaCTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCAc                                                                                                                                               <  1:503964/250‑1 (MQ=255)                                                                                                     aaaCGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGgc                                                                                                                                          <  1:302326/250‑1 (MQ=255)                                                                                                          gCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTgg                                                                                                                                    >  1:357938/1‑251 (MQ=255)                                                                                                               aCATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGc                                                                                                                                                 >  1:267034/1‑233 (MQ=255)                                                                                                               aCATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGt                                                                                                                               >  1:220528/1‑251 (MQ=255)                                                                                                                      cTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGATAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAatat                                                                                                                        <  1:812515/251‑1 (MQ=255)                                                                                                                            cgcCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTc                                                                                                                   <  1:344826/250‑1 (MQ=255)                                                                                                                                  gccatAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACgg                                                                                                                                                        <  1:127257/207‑1 (MQ=255)                                                                                                                                  gccatAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAg                                                                                                                      <  1:745967/241‑1 (MQ=255)                                                                                                                                  gccatAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCa                                                                                                            >  1:122711/1‑251 (MQ=255)                                                                                                                                     atAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGAt                                                                                                         >  1:576831/1‑251 (MQ=255)                                                                                                                                     atAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGAt                                                                                                         >  1:475632/1‑251 (MQ=255)                                                                                                                                            aCCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGAtttt                                                                                                                                                                                                                             <  1:340237/128‑1 (MQ=255)                                                                                                                                               cagcagGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATa                                                                                                   >  1:153944/1‑247 (MQ=255)                                                                                                                                                agcagGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAatat                                                                                                                        <  1:315182/225‑1 (MQ=255)                                                                                                                                                      aGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAg                                                                                                                                                                             <  1:577327/166‑1 (MQ=255)                                                                                                                                                        cTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTg                                                                                       >  1:590495/1‑250 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAAt                                                                                                                                                                               <  1:239518/152‑1 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGc                                                                                                                                                 >  1:766907/1‑182 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTc                                                                                                                   >  1:86025/1‑212 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAAt                                                                                                    <  1:520551/227‑1 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAAt                                                                                                    <  1:409748/227‑1 (MQ=255)                                                                                                                                                                  gggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCa                                                                            >  1:775596/1‑251 (MQ=255)                                                                                                                                                                   ggCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGcctaa                                                                           >  1:504503/1‑248 (MQ=255)                                                                                                                                                                      aaTAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGaa                                                                        <  1:594086/251‑1 (MQ=255)                                                                                                                                                                        tAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGAttt                                                                                                                                                                                                                              >  1:627209/1‑99 (MQ=255)                                                                                                                                                                        tAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACgg                                                                                                                                                        >  1:880491/1‑169 (MQ=255)                                                                                                                                                                        tAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAatat                                                                                                                        <  1:508299/201‑1 (MQ=255)                                                                                                                                                                         aacaacGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGc                                                                     <  1:825835/251‑1 (MQ=255)                                                                                                                                                                                ccTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGAc                                                                                                                                                                                   >  1:786357/1‑134 (MQ=255)                                                                                                                                                                                ccTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGaa                                                                        >  1:797236/1‑241 (MQ=255)                                                                                                                                                                                     aaaa‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCa                                                         <  1:565147/251‑1 (MQ=255)                                                                                                                                                                                           aatCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAgg                                                    <  1:722320/251‑1 (MQ=255)                                                                                                                                                                                           aatCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAgg                                                    <  1:228150/251‑1 (MQ=255)                                                                                                                                                                                             tCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGa                                                                                                                                                                                                                         >  1:597408/1‑84 (MQ=255)                                                                                                                                                                                             tCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGc                                                                                                                                                 <  1:435237/156‑1 (MQ=255)                                                                                                                                                                                             tCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGgc                                                                                                                                          <  1:819869/163‑1 (MQ=255)                                                                                                                                                                                             tCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAatat                                                                                                                        <  1:116568/181‑1 (MQ=255)                                                                                                                                                                                             tCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGgag                                                  >  1:43911/1‑251 (MQ=255)                                                                                                                                                                                               aTTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGAt                                                >  1:29473/1‑251 (MQ=255)                                                                                                                                                                                                       gTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGt                                                                                                                     >  1:344436/1‑174 (MQ=255)                                                                                                                                                                                                          aGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGc                                                                                                                                                 <  1:22774/143‑1 (MQ=255)                                                                                                                                                                                                            aaaTCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGa                                     <  1:894801/249‑1 (MQ=255)                                                                                                                                                                                                            aaaTCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGa                                     <  1:420946/249‑1 (MQ=255)                                                                                                                                                                                                            aaaTCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGACGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGa                                     <  1:437772/249‑1 (MQ=255)                                                                                                                                                                                                               tCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTc                                                                                                                   <  1:274593/168‑1 (MQ=255)                                                                                                                                                                                                                             gttgAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGc                   <  1:464297/250‑1 (MQ=255)                                                                                                                                                                                                                             gttgAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGc                   <  1:130662/250‑1 (MQ=255)                                                                                                                                                                                                                             gttgAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCa                  >  1:141182/1‑251 (MQ=255)                                                                                                                                                                                                                             gttgAAAAATAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGAGGAATATAGTTc                                                                                                                   >  1:115724/1‑154 (MQ=255)                                                                                                                                                                                                                                 aaaaaTAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATc                                                          >  1:476586/1‑207 (MQ=255)                                                                                                                                                                                                                                   aaaTAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGAtgt                                                                                                                                                                                                            <  1:709662/59‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGaa                                                                        >  1:187146/1‑191 (MQ=255)                                                                                                                                                                                                                                   aaaTAAGACCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGttt            >  1:876349/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGTTTTACGt       <  1:366110/249‑1 (MQ=255)                                                                                                                                                                                                                                          aCCAAGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGTTTTACGt       <  1:603605/249‑1 (MQ=255)                                                                                                                                                                                                                                             aaGCTGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGTTTTACGTAATcc  <  1:724643/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CTGAAAGGAATTTAACGCGCTCACCTGCTGAATATTGGCAAAGGGCGTAAAAATGATCGTGCAGCCGACATAAATTATGAGCAAAATTTGTTGCGAACTGAAACGGCTCAACATCAGCTTTTGCGCCATGCCATAGGCAACCCAGCAGGAGCTGGCGCATAGGGCAATAACAACGCCTTTAAAA‑TAATCATTGAGCTGTAAGAAATCACTAAAGCGTTGGTTGAAAAATAAGACCAAGCCGAGAATTAAAATAACCAAACCGATTTTTTGATGGAGCCCGATGTGTTCTTTAAACACAAACACACCGACAAATAGCATGATAAACGAGGATAACGGACTGAGCACTTGGCTGCTGGTCGGTGGAATATAGTTCAGCGCCAGATTAAATAAGAAGAAATTTGCCGATAGCCCAAGAATGCCCAGCATAATCAAAAGGAGATAACGCTGACGAAGTGCGGTGGGTTTTGGCAGCGTTTTACGTAATCC  >  1/312906‑313391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑