Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,690,913	T→C	intergenic (+89/+300)	unknown → / ← unknown	hypothetical protein/virulence‑associated protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,690,913	0	T	C	100.0%	140.0 / NA	51	intergenic (+89/+300)	unknown/unknown	hypothetical protein/virulence‑associated protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/33);  total (18/33)

CCTGTTGGTAGGGTGCAACTTGTTGCACCGATTTTAAATATTTTGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCTGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAACAGTTACAATG  >  1/1690666‑1691152                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                ccTGTTGGTAGGGTGCAACTTGTTGCACCGATTTTAAATATTTTGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGtt                                                                                                                                                                                                                                              >  1:470055/1‑251 (MQ=255)      tGGTAGGGTGCAACTTGTTGCACCGATTTTAAATATTTTGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACgg                                                                                                                                                                                                                                         <  1:374398/251‑1 (MQ=255)                    ttgttgCACCGATTTTAAATATTTTGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCaa                                                                                                                                                                                                                           >  1:850179/1‑251 (MQ=255)                                            tGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGcc                                                                                                                                                                                                   <  1:637788/251‑1 (MQ=255)                                              gTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGAc                                                                                                                                                                                                                                    <  1:410216/216‑1 (MQ=255)                                                            aCCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACg                                                                                                                                                                                               >  1:347698/1‑239 (MQ=255)                                                             cccTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGt                                                                                                                                                                                  >  1:44256/1‑251 (MQ=255)                                                               cTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGc                                                                                                                                                                                                                             >  1:170311/1‑206 (MQ=255)                                                                 aCATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGt                                                                                                                                                                                                                      <  1:657567/211‑1 (MQ=255)                                                                 aCATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAggg                                                                                                                                                                                    >  1:106779/1‑245 (MQ=255)                                                                  cATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGtgagta                                                                                                                                                                             <  1:211716/251‑6 (MQ=255)                                                                            cgcCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGttt                                                                                                                                                                    >  1:398401/1‑250 (MQ=255)                                                                                                tttCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCa                                                                                                                                                                             <  1:136431/221‑1 (MQ=255)                                                                                                     aTTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGaa                                                                                                                                          <  1:851543/251‑1 (MQ=255)                                                                                                        gTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAgg                                                                                                                                                                                     <  1:360850/205‑1 (MQ=255)                                                                                                                    aCTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGttt                                                                                                                                                                                                                                             <  1:341011/137‑1 (MQ=255)                                                                                                                     cTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAAt                                                                                                                          <  1:821476/251‑1 (MQ=255)                                                                                                                       tGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAAttt                                                                                                                        <  1:83277/251‑1 (MQ=255)                                                                                                                       tGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAAttt                                                                                                                        <  1:532777/251‑1 (MQ=255)                                                                                                                         aTTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACggg                                                                                                                                                                                                                                        <  1:715573/137‑1 (MQ=255)                                                                                                                          ttCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAggaggcaagatcc                                                                                                                     <  1:260431/251‑12 (MQ=255)                                                                                                                                        aGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGc                                                                                                                                                                              >  1:742038/1‑180 (MQ=255)                                                                                                                                        aGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTcac                                                                                                        <  1:344856/250‑1 (MQ=255)                                                                                                                                          tCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCTCAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAg                                                                                                     <  1:475693/251‑1 (MQ=255)                                                                                                                                          tCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTcaca                                                                                                       <  1:389676/249‑1 (MQ=255)                                                                                                                                             gCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAg                                                                                                     <  1:464262/248‑1 (MQ=255)                                                                                                                                                 aCGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAAtt                                                                                                                         <  1:575272/224‑1 (MQ=255)                                                                                                                                                    tgtgCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCtt                                                                                            <  1:34102/250‑1 (MQ=255)                                                                                                                                                     gtgCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGc                                                                                                                                                                              <  1:49057/167‑1 (MQ=255)                                                                                                                                                        ccTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTaaaaa                                                                                       >  1:844594/1‑251 (MQ=255)                                                                                                                                                            ataAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTcaca                                                                                                       >  1:30540/1‑231 (MQ=255)                                                                                                                                                             taAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTgag                                                                                  >  1:180241/1‑251 (MQ=255)                                                                                                                                                                            aTTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAg                                                                                                                                         >  1:707779/1‑181 (MQ=255)                                                                                                                                                                               aGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAggg                                                                                                                               <  1:653669/188‑1 (MQ=255)                                                                                                                                                                               aGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAggg                                                                >  1:658484/1‑251 (MQ=255)                                                                                                                                                                                 cACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGc                                                               <  1:465208/250‑1 (MQ=255)                                                                                                                                                                                     aGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAg                                                                                                     >  1:315861/1‑208 (MQ=255)                                                                                                                                                                                       tCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGccc                                                        <  1:211182/251‑1 (MQ=255)                                                                                                                                                                                                        ttCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACg                                       <  1:429898/251‑1 (MQ=255)                                                                                                                                                                                                             aaCATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAgggg                                  <  1:773345/251‑1 (MQ=255)                                                                                                                                                                                                              aCATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGtgagt                                                                                                                                                                              >  1:734186/1‑106 (MQ=255)                                                                                                                                                                                                               cATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAg                                >  1:17128/1‑251 (MQ=255)                                                                                                                                                                                                                 ttATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGc                               >  1:744363/1‑250 (MQ=255)                                                                                                                                                                                                                     aaTTTATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGt                          <  1:418420/251‑1 (MQ=255)                                                                                                                                                                                                                         tATTGATATCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGttttt                      <  1:106727/251‑1 (MQ=255)                                                                                                                                                                                                                                 tCTTAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATaa              >  1:751922/1‑251 (MQ=255)                                                                                                                                                                                                                                   ttAAAATATCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAAc            <  1:418965/251‑1 (MQ=255)                                                                                                                                                                                                                                         tatCTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAACAGTTAc      <  1:59398/251‑1 (MQ=255)                                                                                                                                                                                                                                            cTTGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTG‑CCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAACAGTTACAATg  <  1:137484/251‑1 (MQ=255)                                                                                                                                                                                                                                             ttGCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAACAGTTACAATg  <  1:64470/251‑1 (MQ=255)                                                                                                                                                                                                                                               gCCTTCCTCCGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAgggg                                                                                                                                                                                   <  1:52328/72‑1 (MQ=255)                                                                                                                                                                                                                                                   tcctccGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTc                                                                                                          <  1:31057/141‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CCTGTTGGTAGGGTGCAACTTGTTGCACCGATTTTAAATATTTTGGTGCAACAAGTTGCACCCTACATAACGACGCGCCAAAAGTGCGGTCGTTTTTTCAATTGTTTTTTAATTCACTTGATTCATACCGCCGCGAGTCTGCCGACGTGTGCCTTATAAATTTAGTGAGCCATTAGCACGAGTCGGGAAACCACAGCTGTTCGAAACATTATAATTTATTGATATCTTAAAATATCTTGCCTTCCTCTGTTTACGGGGGACAAAACGCAATGCGTTTTGAGCGTTGGTTCTGCCAACGACCCCAAAGGGGTAAGCAAGTGAGTTTCCGAGCTTGCGAATAACGTACCCGAAGGGCGGAAGGGGGAATTTATCTCGATGTATTCACAAGATCATGCTTAAAAATTGAGATTTTGCCCCCCTCAGGGCTTCGCCCAGCTCCCCCCGCGAACGAGGGGAGCCAAGTTTTTTTATATAAACAGTTACAATG  >  1/1690666‑1691152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑