Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	312,051	C→T	A165T (GCG→ACG)	unknown ←	Uncharacterized inner membrane transporter YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	312,051	0	C	T	100.0%	159.5 / NA	59	A165T (GCG→ACG)	unknown	Uncharacterized inner membrane transporter YhbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/25);  total (34/25)

GACTTTGGATACTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGCAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAACTGTTAAATAAGAAGA  >  1/311809‑312293                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gACTTTGGATACTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCa                                                                                                                                                                                                                                            >  1:469752/1‑251 (MQ=255)   cTTTGGATACTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACg                                                                                                                                                                                                                                          <  1:845689/251‑1 (MQ=255)    tttGGATACTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGc                                                                                                                                                                                                                                         <  1:786136/251‑1 (MQ=255)           aCTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAg                                                                                                                                                                                                                                  <  1:552254/251‑1 (MQ=255)            cTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAg                                                                                                                                                                                                                                  >  1:196788/1‑250 (MQ=255)                    cGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGt                                                                                                                                                                                                                         >  1:850504/1‑251 (MQ=255)                    cGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGt                                                                                                                                                                                                                         <  1:386019/251‑1 (MQ=255)                      gTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCAc                                                                                                                                                                                                                                               >  1:30939/1‑227 (MQ=255)                      gTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCAc                                                                                                                                                                                                                                               <  1:206272/227‑1 (MQ=255)                       tttAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAg                                                                                                                                                                                                                       <  1:144063/250‑1 (MQ=255)                             gCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAgg                                                                                                                                                                                                                 <  1:868114/250‑1 (MQ=255)                                 ccGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGttt                                                                                                                                                                                                            >  1:866619/1‑251 (MQ=255)                                          cgcCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAg                                                                                                                                                                                                                       >  1:891477/1‑231 (MQ=255)                                               tAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATcc                                                                                                                                                                                                       <  1:260357/242‑1 (MQ=255)                                               tAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGaaaa                                                                                                                                                                                               >  1:60647/1‑250 (MQ=255)                                                  ccGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCAc                                                                                                                                                                                                                                               >  1:313088/1‑199 (MQ=255)                                                    gAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATc                                                                                                                                                                                                        >  1:743206/1‑236 (MQ=255)                                                       tAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAAtt                                                                                                                                                                                      <  1:688699/251‑1 (MQ=255)                                                         gagTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATaa                                                                                                                                                                                                                                   >  1:855870/1‑204 (MQ=255)                                                                      gcaATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAaga                                                                                                                                                                       >  1:252653/1‑251 (MQ=255)                                                                           aaaTGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACg                                                                                                                                                                                                                                          >  1:824600/1‑179 (MQ=255)                                                                                     gcCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATaa                                                                                                                                                        >  1:584298/1‑251 (MQ=255)                                                                                     gcCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATaa                                                                                                                                                        >  1:381276/1‑251 (MQ=255)                                                                                     gcCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATaa                                                                                                                                                        >  1:469172/1‑251 (MQ=255)                                                                                       cTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGc                                                                                                                                                      <  1:26656/251‑1 (MQ=255)                                                                                              cAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGa                                                                                                                                               >  1:54563/1‑251 (MQ=255)                                                                                                 tGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGAc                                                                                                                                              <  1:610460/249‑1 (MQ=255)                                                                                                 tGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGAc                                                                                                                                              <  1:588989/249‑1 (MQ=255)                                                                                                 tGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGAc                                                                                                                                              <  1:802251/249‑1 (MQ=255)                                                                                                    ggTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGa                                                                                                                                                                                                  <  1:293249/194‑1 (MQ=255)                                                                                                          tCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAg                                                                                                                                                                                                                       <  1:644772/167‑1 (MQ=255)                                                                                                          tCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAAttt                                                                                                                                                                                     <  1:869871/201‑1 (MQ=255)                                                                                                          tCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGAttt                                                                                                                                   >  1:608668/1‑251 (MQ=255)                                                                                                           cccTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGAtttt                                                                                                                                  >  1:711197/1‑251 (MQ=255)                                                                                                                                     gggCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAAttt                                                                                                                <  1:540480/243‑1 (MQ=255)                                                                                                                                      ggCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCAc                                                                                                                                                                                                                                               >  1:228841/1‑115 (MQ=255)                                                                                                                                                            cGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAg                                                                                  >  1:507585/1‑250 (MQ=255)                                                                                                                                                              aaataaatCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCAt                                                                               >  1:650762/1‑251 (MQ=255)                                                                                                                                                                               aaTTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAagg                                                                       >  1:342787/1‑242 (MQ=255)                                                                                                                                                                               aaTTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAAt                                                              >  1:330784/1‑251 (MQ=255)                                                                                                                                                                                                         aCATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCAc                                                                                                                                                                                                                                               >  1:251423/1‑48 (MQ=255)                                                                                                                                                                                                         aCATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATg                                                                                    >  1:87397/1‑203 (MQ=255)                                                                                                                                                                                                          cATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGtt                                   >  1:285512/1‑251 (MQ=255)                                                                                                                                                                                                                tttttGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAatat                             <  1:538549/251‑1 (MQ=255)                                                                                                                                                                                                                tttttGCGCCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAatat                             <  1:209602/251‑1 (MQ=255)                                                                                                                                                                                                                      cgcCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAatat                             >  1:690766/1‑245 (MQ=255)                                                                                                                                                                                                                      cgcCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAAttt                        <  1:651793/250‑1 (MQ=255)                                                                                                                                                                                                                      cgcCATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAAttt                        <  1:234538/250‑1 (MQ=255)                                                                                                                                                                                                                        ccATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACgg                                         >  1:414817/1‑231 (MQ=255)                                                                                                                                                                                                                         cATACCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATg                    <  1:709109/251‑1 (MQ=255)                                                                                                                                                                                                                            aCCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAAt                                                              >  1:282220/1‑206 (MQ=255)                                                                                                                                                                                                                            aCCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGaa                  >  1:642963/1‑250 (MQ=255)                                                                                                                                                                                                                            aCCGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAAc                 <  1:57872/251‑1 (MQ=255)                                                                                                                                                                                                                             ccGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAatat                             >  1:283306/1‑238 (MQ=255)                                                                                                                                                                                                                             ccGTAGGCCACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGaa                  >  1:419746/1‑249 (MQ=255)                                                                                                                                                                                                                                     cACCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTc                                  <  1:140386/225‑1 (MQ=255)                                                                                                                                                                                                                                      aCCCATACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAACTGTTAAATaa       <  1:374821/251‑1 (MQ=255)                                                                                                                                                                                                                                           tACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAACTGTTAAATAagaaga  >  1:104635/1‑251 (MQ=255)                                                                                                                                                                                                                                           tACCAGCGTAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAACTGTTAAATAagaaga  >  1:209349/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GACTTTGGATACTTCCCAGCGGTTTAGGGCTTCCGCGTAAGCGCCGTAACCGAATAGAGTGTTTAAGCAGCAATAAATGAAGCAGCCTAGCGCCAGTGGGGTTAATCCCTGAACTTGTGAAAATTCGGCGAAGGGCGTAAAGACGATGGCGCAGCCGAAATAAATCATTAATAAAATTTGTTGGGCGCTGAATTTTCGTAACATTAATTTTTGCGCCATACCGTAGGCCACCCATACCAGCGCAGCACTCACGCTGATAAGCACGCCGGTAGTGTAGGTGTTTAATCCGTTGAAAACCTCTAATTTATCGTTGAAAAAGAGTCCCAAACCGATAAGCAATAAGACCAAGCCGATTTTTTGATGCAAGCCTAATTTTTCCTTAAAAATCACTACGCCGCAAATGAGCATGCCGAAGGAGGATAAATGAATAAAAATTTGTGCAACGGAGGGTTCAATATAATTTAATGAACTGTTAAATAAGAAGA  >  1/311809‑312293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑