Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	310,798	T→C	S264S (TCA→TCG)	unknown ←	GTP‑binding protein Obg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	310,798	0	T	C	100.0%	194.6 / NA	67	S264S (TCA→TCG)	unknown	GTP‑binding protein Obg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/38);  total (29/38)

GCGGGTGGGCTTCCAGGAAGTCCATAATATCGCGGCACAGTTGCGGGGCGTTTTTACCGGTGGCGGCGGAAATTAAATAATAACCTTCCTCCCGGCCCAGACGTTCGGTGATCTCTTTCGCTCGCGCATGGGCTTCTTCATCACTGAGCGTATCGATTTTGTTGAACACCAGCCAGCGCGGTTTTTCCGCCAAGGACTCGCTGTCGCTGTATTGGAACAATTCTGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTAAACGGATAATCGGCGA  >  1/310575‑311033                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            ttttgcgcgggtgggcttccaggaagtccataatatcgcggctcagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacgccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATccgc                                                                                                                                                                                                                   <  1:78620‑M2/40‑1 (MQ=255)        cgggtgggcttccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTgg                                                                                                                                                                                                           >  1:130593‑M2/204‑251 (MQ=255)          ggtgggcttccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGaa                                                                                                                                                                                                         >  1:179017‑M2/202‑251 (MQ=255)           gtgggcttccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGaa                                                                                                                                                                                                         <  1:708674‑M2/50‑1 (MQ=255)                  tccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataatagccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAAt                                                                                                                                                                                                                                  <  1:491160‑M2/25‑1 (MQ=255)                  tccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAAtcatca                                                                                                                                                                                                   <  1:383971‑M2/56‑1 (MQ=255)                  tccaggaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAAt                                                                                                                                                                                                 >  1:104175‑M2/194‑251 (MQ=255)                       gaagtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTg                                                                                                                                                                                               <  1:807703‑M2/60‑1 (MQ=255)                          gtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGa                                                                                                                                                                                         >  1:341828‑M2/186‑251 (MQ=255)                          gtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGa                                                                                                                                                                                         >  1:3875‑M2/186‑251 (MQ=255)                          gtccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGa                                                                                                                                                                                         >  1:92549‑M2/186‑251 (MQ=255)                            ccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGata                                                                                                                                                                                       >  1:720892‑M2/184‑251 (MQ=255)                            ccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGata                                                                                                                                                                                       >  1:276682‑M2/184‑251 (MQ=255)                            ccataatatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGata                                                                                                                                                                                       <  1:417614‑M2/68‑1 (MQ=255)                                 atatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgggcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATccac                                                                                                                                                                                  <  1:89276‑M2/73‑1 (MQ=255)                                 atatcgcggcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATccac                                                                                                                                                                                  <  1:207431‑M2/73‑1 (MQ=255)                                         gcacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATccac                                                                                                                                                                                  <  1:474514‑M2/73‑1 (MQ=255)                                          cacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGaa                                                                                                                                                                          <  1:214399‑M2/81‑1 (MQ=255)                                          cacagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGaa                                                                                                                                                                          <  1:535325‑M2/81‑1 (MQ=255)                                            cagttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCa                                                                                                                                                                       >  1:100327‑M2/168‑251 (MQ=255)                                               ttgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATc                                                                                                                                                                        <  1:520852‑M2/83‑1 (MQ=255)                                                tgcggggcgtttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAacac                                                                                                                                                                   <  1:667067‑M2/88‑1 (MQ=255)                                                         tttttaccggtggcggcggaaattaaataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCaa                                                                                                                                                                      >  1:269327‑M2/155‑239 (MQ=255)                                                                                   ataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATccgc                                                                                                                                                                                                                   <  1:844117‑M2/40‑1 (MQ=255)                                                                                   ataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATa                                                                                                                                 >  1:572816‑M2/129‑250 (MQ=255)                                                                                   ataataaccttcctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggaatcggtgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATa                                                                                                                                 >  1:611582‑M2/129‑250 (MQ=255)                                                                                               ctcccggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTc                                                                                                                    <  1:795238‑M2/135‑1 (MQ=255)                                                                                                   cggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGTTAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTc                                                                                                                <  1:409037‑M2/139‑1 (MQ=255)                                                                                                   cggcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTc                                                                                                                <  1:544289‑M2/139‑1 (MQ=255)                                                                                                     gcccagacgttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCg                                                                                                              >  1:765280‑M2/111‑251 (MQ=255)                                                                                                             gttcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAgtg                                                                                                                                                 <  1:554295‑M2/106‑1 (MQ=255)                                                                                                               tcggtgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTcc                                                                                                               <  1:813779‑M2/140‑1 (MQ=255)                                                                                                                   tgatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAAtt                                                                                                <  1:321556‑M2/155‑1 (MQ=255)                                                                                                                    gatctctttcgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAgg                                                                                                                                          <  1:298575‑M2/113‑1 (MQ=255)                                                                                                                             cgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGaa                                                                                      <  1:773411‑M2/165‑1 (MQ=255)                                                                                                                             cgctcgcgcatgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGaa                                                                                      <  1:554037‑M2/165‑1 (MQ=255)                                                                                                                                     catgggcttcttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCg                                                                              >  1:386385‑M2/79‑251 (MQ=255)                                                                                                                                              cttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGAtt                                                                                                                                                                                                                                         >  1:102461‑M2/70‑87 (MQ=255)                                                                                                                                              cttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAAtt                                                                                                <  1:890261‑M2/155‑1 (MQ=255)                                                                                                                                              cttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAg                                                                                             >  1:801651‑M2/70‑227 (MQ=255)                                                                                                                                              cttcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACaaaa                                                                     >  1:847607‑M2/70‑251 (MQ=255)                                                                                                                                                tcatcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACaaaa                                                                     >  1:661147‑M2/68‑249 (MQ=255)                                                                                                                                                 catcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTg                                                                  <  1:134771‑M2/185‑1 (MQ=255)                                                                                                                                                   tcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGcc                                                                                                                         <  1:558328‑M2/130‑1 (MQ=255)                                                                                                                                                   tcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCg                                                                >  1:830120‑M2/65‑251 (MQ=255)                                                                                                                                                   tcactgagcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCg                                                                >  1:413279‑M2/65‑251 (MQ=255)                                                                                                                                                          gcgtatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCa                                                         >  1:291550‑M2/58‑251 (MQ=255)                                                                                                                                                             tatcgattttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAg                                                                                                                                                   <  1:295228‑M2/104‑1 (MQ=255)                                                                                                                                                                  attttgttgaacaccagccagcgcggtttttccgccaaggacttgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTTATATCCACCAAATGAATCAACACGCGGCAACGTTCCAATTTTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCAATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACttt                                                 >  1:712235‑M2/50‑251 (MQ=255)                                                                                                                                                                  attttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACttt                                                 >  1:201247‑M2/50‑251 (MQ=255)                                                                                                                                                                     ttgttgaacaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAAc                                                                    <  1:602564‑M2/183‑1 (MQ=255)                                                                                                                                                                            acaccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTcacc                                             >  1:336240‑M2/40‑245 (MQ=255)                                                                                                                                                                             caccagccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGa                                                                             <  1:321178‑M2/174‑1 (MQ=255)                                                                                                                                                                                 agccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTaaa                                    >  1:509153‑M2/35‑249 (MQ=255)                                                                                                                                                                                 agccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTaaa                                    >  1:91569‑M2/35‑249 (MQ=255)                                                                                                                                                                                   ccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCGCCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACt                                  <  1:285764‑M2/217‑1 (MQ=255)                                                                                                                                                                                   ccagcgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAAc                                   <  1:527201‑M2/216‑1 (MQ=255)                                                                                                                                                                                       cgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCTTCTACTTTCACCACGCCTAAACTTGGt                              <  1:111792‑M2/221‑1 (MQ=255)                                                                                                                                                                                       cgcggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTAc                            <  1:877077‑M2/223‑1 (MQ=255)                                                                                                                                                                                         cggtttttccgccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTAcc                           <  1:643003‑M2/224‑1 (MQ=255)                                                                                                                                                                                                   gccaaggactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTaaa                >  1:678171‑M2/17‑251 (MQ=255)                                                                                                                                                                                                         gactcgctgtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCTACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTAAACGGAt           <  1:432900‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                 gtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACtt                                                  <  1:865511‑M2/201‑1 (MQ=255)                                                                                                                                                                                                                 gtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTcacca                                            <  1:349862‑M2/207‑1 (MQ=255)                                                                                                                                                                                                                 gtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTAc                            <  1:42952‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                 gtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTAAACGGATAATCGGCGa  >  1:364565‑M2/3‑251 (MQ=255)                                                                                                                                                                                                                 gtATTGGAACAATTCCGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTAAACGGATAATCGGCGa  >  1:473217‑M2/3‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            GCGGGTGGGCTTCCAGGAAGTCCATAATATCGCGGCACAGTTGCGGGGCGTTTTTACCGGTGGCGGCGGAAATTAAATAATAACCTTCCTCCCGGCCCAGACGTTCGGTGATCTCTTTCGCTCGCGCATGGGCTTCTTCATCACTGAGCGTATCGATTTTGTTGAACACCAGCCAGCGCGGTTTTTCCGCCAAGGACTCGCTGTCGCTGTATTGGAACAATTCTGATTCGATAATCGCCACGTTATCCGCCGGGTTGGAATCATCAATTGGGTTGATATCCACCAAATGAATCAACACGCGGCAACGTTCCAAGTGTTTCAGGAAGCGGATACCCAAGCCTGCTCCTTCCGATGCGCCTTCAATTAAGCCAGGAATGTCGGCGATCACAAAACTGCGATTGTCATCTACTTTCACCACGCCTAAACTTGGTACCAAGGTGGTAAACGGATAATCGGCGA  >  1/310575‑311033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑