Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,673,513	G→A	L9F (CTT→TTT)	unknown ←	Excinuclease ATPase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,673,513	0	G	A	100.0%	218.7 / NA	81	L9F (CTT→TTT)	unknown	Excinuclease ATPase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/34);  total (47/34)

TGCTTAACGGCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAGGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGAAAT  >  1/1673266‑1673750                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              tGCTTAACGGCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGt                                                                                                                                                                                                                                            <  1:719255/251‑1 (MQ=255)      aaCGGCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAAtt                                                                                                                                                                                                                                       <  1:301287/251‑1 (MQ=255)       aCGGCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAAttt                                                                                                                                                                                                                                      <  1:830734/251‑1 (MQ=255)          gCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAg                                                                                                                                                                                                                                    <  1:147398/250‑1 (MQ=255)          gCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGt                                                                                                                                                                                                                                   >  1:1525/1‑251 (MQ=255)            aGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGtca                                                                                                                                                                                                                                 >  1:209143/1‑251 (MQ=255)              aCAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGtcatc                                                                                                                                                                                                                               <  1:11625/251‑1 (MQ=255)                                                                                cAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTa                                                                                                                                                             >  1:335577/1‑251 (MQ=255)                                                                                       gTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATaa                                                                                                                                                      >  1:468043/1‑251 (MQ=255)                                                                                                 acTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATg                                                                                                                                            >  1:400392/1‑251 (MQ=255)                                                                                                  cTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAAt                                                                                                                                                                                                                 >  1:304744/1‑181 (MQ=255)                                                                                                            tGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCgg                                                                                                                                  <  1:620796/250‑1 (MQ=255)                                                                                                                ggTTTACCTAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGAt                                                                                                                                                                                                      <  1:591238/178‑1 (MQ=255)                                                                                                                ggTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCgg                                                                                                                                  <  1:662624/246‑1 (MQ=255)                                                                                                                         aaGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCtt                                                                                                                    >  1:749137/1‑251 (MQ=255)                                                                                                                                 ttttACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAAc                                                                                                              <  1:236159/249‑1 (MQ=255)                                                                                                                                          cGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCAt                                                                                                                                                                  >  1:446063/1‑188 (MQ=255)                                                                                                                                            ggTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGAt                                                                                                                                                                                                      >  1:627820/1‑150 (MQ=255)                                                                                                                                            ggTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTa                                                                                                 >  1:376871/1‑251 (MQ=255)                                                                                                                                            ggTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTa                                                                                                 >  1:502420/1‑251 (MQ=255)                                                                                                                                                gAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGaa                                                                                                                                                  >  1:27695/1‑198 (MQ=255)                                                                                                                                                gAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAAt                                                                                             >  1:14074/1‑251 (MQ=255)                                                                                                                                                             gCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTa                                                                                                                                                             <  1:873888/174‑1 (MQ=255)                                                                                                                                                              cTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAAttt                                                                                                                                                                                                                                      >  1:143606/1‑100 (MQ=255)                                                                                                                                                              cTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTa                                                                               >  1:399503/1‑251 (MQ=255)                                                                                                                                                              cTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTa                                                                               >  1:506718/1‑251 (MQ=255)                                                                                                                                                              cTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTa                                                                               >  1:713074/1‑251 (MQ=255)                                                                                                                                                                tCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATc                                                                             <  1:115297/251‑1 (MQ=255)                                                                                                                                                                   ggggAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCttt                                                                          >  1:631982/1‑251 (MQ=255)                                                                                                                                                                   ggggAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCttt                                                                          >  1:613725/1‑251 (MQ=255)                                                                                                                                                                        aTTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCAt                                                                                                                                                                  >  1:891574/1‑158 (MQ=255)                                                                                                                                                                        aTTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATAc                                                                                                                                               <  1:58617/177‑1 (MQ=255)                                                                                                                                                                          tCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTg                                                                                                                       >  1:468151/1‑199 (MQ=255)                                                                                                                                                                          tCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTAttt                                                                   >  1:863148/1‑251 (MQ=255)                                                                                                                                                                                  cTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCAt                                                                                                                                                                  >  1:187310/1‑148 (MQ=255)                                                                                                                                                                                  cTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTCCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGc                                                                                                                      >  1:34880/1‑192 (MQ=255)                                                                                                                                                                                    ttGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGAt                                                                                                                                                                                                      <  1:532708/110‑1 (MQ=255)                                                                                                                                                                                    ttGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTa                                                                      >  1:631153/1‑238 (MQ=255)                                                                                                                                                                                      gAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGcc                                                                                                       <  1:717937/203‑1 (MQ=255)                                                                                                                                                                                          atCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAg                                                                                                                                                                          <  1:580376/132‑1 (MQ=255)                                                                                                                                                                                           tCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAAc                                                                                                                                                                                    <  1:432713/121‑1 (MQ=255)                                                                                                                                                                                           tCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACAt                                                                                                                                             <  1:247063/160‑1 (MQ=255)                                                                                                                                                                                           tCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCA‑TTTAATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTgaaag                                                 >  1:812321/1‑251 (MQ=255)                                                                                                                                                                                             aTAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCgg                                                                                                                                  >  1:200315/1‑169 (MQ=255)                                                                                                                                                                                             aTAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCgg                                                                                                                                  <  1:858542/169‑1 (MQ=255)                                                                                                                                                                                             aTAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATc                                                                             >  1:680386/1‑222 (MQ=255)                                                                                                                                                                                             aTAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTgaaaga                                                >  1:610869/1‑251 (MQ=255)                                                                                                                                                                                                gTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGaaaa                                             >  1:818795/1‑251 (MQ=255)                                                                                                                                                                                                gTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGaaaa                                             >  1:205018/1‑251 (MQ=255)                                                                                                                                                                                                  gatgaGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCgg                                                                                                                                  <  1:340976/164‑1 (MQ=255)                                                                                                                                                                                                   ctcacctccaacACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAgg                                          >  1:624537/12‑251 (MQ=255)                                                                                                                                                                                                     gaGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCa                                        >  1:200484/1‑251 (MQ=255)                                                                                                                                                                                                       gTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAAt                                      >  1:46919/1‑251 (MQ=255)                                                                                                                                                                                                          gTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATtgt                                   >  1:201324/1‑251 (MQ=255)                                                                                                                                                                                                          gTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATtg                                    <  1:825722/250‑1 (MQ=255)                                                                                                                                                                                                               aCGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGt                                                                                                                                                                                                   >  1:81714/1‑86 (MQ=255)                                                                                                                                                                                                                  cgGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGta                            <  1:610318/250‑1 (MQ=255)                                                                                                                                                                                                                    gggCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATAc                                                                                                                                               <  1:724266/133‑1 (MQ=255)                                                                                                                                                                                                                    gggCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGtattt                         >  1:693282/1‑251 (MQ=255)                                                                                                                                                                                                                    gggCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGtattt                         >  1:870969/1‑251 (MQ=255)                                                                                                                                                                                                                    gggCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGtattt                         >  1:285008/1‑251 (MQ=255)                                                                                                                                                                                                                      gCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGa                                                                                                                                                                                                                     <  1:42836/61‑1 (MQ=255)                                                                                                                                                                                                                      gCACAGGCGGCGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGtatttta                       >  1:190783/1‑251 (MQ=255)                                                                                                                                                                                                                           ggcggcGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTAttt                                                                   >  1:154571/1‑202 (MQ=255)                                                                                                                                                                                                                           ggcggcGATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTAttttt                  >  1:874644/1‑251 (MQ=255)                                                                                                                                                                                                                               gcgATAAATACAGTCGCAACGGCAAAGGTTAAttt                                                                                                                                                                                                                                      <  1:331966/35‑1 (MQ=255)                                                                                                                                                                                                                               gcgATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCACGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCa                                        <  1:12842/225‑1 (MQ=255)                                                                                                                                                                                                                                 gATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCa                >  1:21538/1‑247 (MQ=255)                                                                                                                                                                                                                                 gATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCa                >  1:16667/1‑247 (MQ=255)                                                                                                                                                                                                                                 gATAAATACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCAc               >  1:659004/1‑248 (MQ=255)                                                                                                                                                                                                                                       tACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTATGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAgg      <  1:447659/251‑1 (MQ=255)                                                                                                                                                                                                                                       tACAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAgg      <  1:23483/251‑1 (MQ=255)                                                                                                                                                                                                                                         cAGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGa     <  1:641437/250‑1 (MQ=255)                                                                                                                                                                                                                                          aGTCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGc                                         >  1:84896/1‑213 (MQ=255)                                                                                                                                                                                                                                            tCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGa     <  1:80810/247‑1 (MQ=255)                                                                                                                                                                                                                                            tCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGa     <  1:869355/247‑1 (MQ=255)                                                                                                                                                                                                                                            tCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGa     <  1:611094/247‑1 (MQ=255)                                                                                                                                                                                                                                            tCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGAAAt  <  1:869856/250‑1 (MQ=255)                                                                                                                                                                                                                                            tCGCAACGGCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGCCAAAGGa     <  1:226621/247‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCAt                                                                                                                                                                  <  1:238798/83‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCAAAGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTg             >  1:896641/1‑232 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              TGCTTAACGGCAGACAAGAAAGCGCGTTTGCAGGCTTTTTCGTCAGATTTGTTGGCAGCATTGGTTTTCTTGTTGGTGACAGCATTGTCGACCACCACTTTACCCGGTGCCGGTTTACCGAAGTAAAGTTTTACATTCGGGTTGAGGAAACGCTGTGCTTCAGGGGAATTCAATACACTTTGAATATCATAGTGATGAGTGGTATCACGCGGGGCACAGGCGGCGATAAATACAGTCGCAACGGCAAGGGTTAATTTAGTCATCAGTTTCATGAAAATCTCCTTTTGATAGTAAAAAAGATGAAAACCGTACGTAAGTATAGCATTTTTATTGATAACGAATACATGGTTCTTGCGGTGCGCCAATTGCTTTCGAACCGGAGCCTAGGTAAAATTGACCCGGTTTTTATCTTTACTATTTTAGCCGAACCGGTGAAAGAAAAAGGCAATTGTGATCGTATTTTATTTTTCACTGTCAAAGGAAAT  >  1/1673266‑1673750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑