Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,670,985	C→T	R142R (CGC→CGT)	unknown →	Diaminopimelate epimerase (EC 5.1.1.7)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,670,985	0	C	T	100.0%	224.3 / NA	81	R142R (CGC→CGT)	unknown	Diaminopimelate epimerase (EC 5.1.1.7)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/52);  total (29/52)

ATCGCATTTTTAACGCGGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGCACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAGCGGCGCT  >  1/1670738‑1671222                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              aTCGCATTTTTAACGCGGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACg                                                                                                                                                                                                                                            >  1:174039/1‑251 (MQ=255)            aaCGCGGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGAAAGCACGAAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGTTTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAAc                                                                                                                                                                                                                                 <  1:883341/251‑1 (MQ=255)              cgcgGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTGGTGACGCTCAAGGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGAAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACgg                                                                                                                                                                                                                               <  1:20421/251‑1 (MQ=255)              cgcgGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACgg                                                                                                                                                                                                                               <  1:773289/251‑1 (MQ=255)                  gACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCG‑‑‑GAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCtgtg                                                                                                                                                                                                                        >  1:99560/1‑251 (MQ=255)                       cggCGAAGTGGCGCAATGGGGCAACGGCGCACGTTGTTTCGCCCGTTTCGGGACGCTCAAAGGCTTAACC‑AAAAAAAAAGACATCGCCGTAAGCACGAAAAAGGGCAAAATGCTGCTGCCCGTG‑AAAAAAGATGGTAACGTGTGGGTAAACAGGGGCGAACCGATTTGGGCGCAGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:71895/248‑1 (MQ=255)                       cAGCGAAGTGGCGCAATGTGGCACCGGCGCACGCTGTTTGGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AAAAAAAAAGACATCGCCGTAAGCACGAAAACCGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:20035/250‑1 (MQ=255)                       cAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:808876/250‑1 (MQ=255)                       cAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:279636/250‑1 (MQ=255)                       cAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACAGGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:279399/250‑1 (MQ=255)                       cAGCGAAGGGGCGCAATGGGGCACCGGCGCGCGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGAAAAATGCTGCGGACCGGG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGGGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       <  1:515628/250‑1 (MQ=255)                          cGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTC‑AAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAgcgc                                                                                                                                                                                                                  <  1:613505/251‑1 (MQ=255)                            aaGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAgcgc                                                                                                                                                                                                                  <  1:230829/250‑1 (MQ=255)                              gTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGt                                                                                                                                                                                                               >  1:756091/1‑251 (MQ=255)                                     aaTGTGGCAACGGCGCCCGCTGTTTGGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACAGCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGGG‑AAAGAAGATGTTAACGTGCGGGTGAACGTGGGCGAACCGATTGGGGAGCGGAATAAAATC‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATg                                                                                                                                                                                                        <  1:131805/251‑1 (MQ=255)                                     aaTGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGAAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATg                                                                                                                                                                                                        <  1:361704/251‑1 (MQ=255)                                      aTGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAg                                                                                                                                                                                                                     >  1:752452/1‑237 (MQ=255)                                       tgtgGCAACGGCGCCCGCTGTTTCGCCCGTTTCGTGACCCTAAAAGCCTTAACC‑AATAAAAAAGACGTCGCCGAAAGCCCGCAAAACGGCAAAATGCTGCTGACCGTA‑AAAGAAAATGGTAACGGGCGGGTGAACATGGGCAAACCGATTGGGGAGCCAAAAAAAATC‑CCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:336546/251‑1 (MQ=255)                                       tgtgGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAGGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGAGAACCGTTTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:146801/251‑1 (MQ=255)                                       tgtgGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:565804/251‑1 (MQ=255)                                       tgtgGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACACCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTACCGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:727593/251‑1 (MQ=255)                                         tgGCAACGGCGCCCGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGc                                                                                                                                                                                                     <  1:696359/250‑1 (MQ=255)                                                    cACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AAAAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCAAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTg                                                                                                                                                                                          <  1:643289/250‑1 (MQ=255)                                                        cTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGc                                                                                                                                                                                                                       >  1:716572/1‑217 (MQ=255)                                                          gTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCgtggtg                                                                                                                                                                                   >  1:7135/1‑251 (MQ=255)                                                          gTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCgtggtg                                                                                                                                                                                   >  1:843847/1‑251 (MQ=255)                                                                cccGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGGG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTg                                                                                                                                                                             <  1:440477/251‑1 (MQ=255)                                                                 ccGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGACCCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTgg                                                                                                                                                                            <  1:624954/251‑1 (MQ=255)                                                                    tttCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTggagg                                                                                                                                                                         >  1:429680/1‑251 (MQ=255)                                                                    tttCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTggagg                                                                                                                                                                         <  1:554669/251‑1 (MQ=255)                                                                    ttacGTGACGCAAAAAGGC‑TAACCAAAAAAAAAAGACATCGCCGTAACCAAGCAAACCGTCAAAATGCGGCTAGCGGTA‑AAAGAAGATGGAAACGTGCGGGTGAACAGGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTggagg                                                                                                                                                                         <  1:703598/248‑1 (MQ=255)                                                                     ttCGTGACGCTCAAAGGCTAAACC‑AATAAAAAAGACATCGCCGTAAGCACGAAAAACGGCAAAATGCTGCTGACGGTG‑AAAGAAGATGGTAACGTGGGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTggagga                                                                                                                                                                        <  1:820226/251‑1 (MQ=255)                                                                             gCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCaaa                                                                                                                                                                >  1:562815/1‑251 (MQ=255)                                                                                  aaGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATg                                                                                                                                                                                                        <  1:588760/206‑1 (MQ=255)                                                                                    ggCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTg                                                                                                                                                                             >  1:846444/1‑231 (MQ=255)                                                                                     gCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAAt                                                                                                                                                        >  1:372439/1‑251 (MQ=255)                                                                                         aaCC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:870751/201‑1 (MQ=255)                                                                                          aacaaaTAAAAAAGACATCGCCGAAAGCACGCAAAACGGCAAAAGGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCAACCCGATTTGGGGGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAAc                                                                                                                                                                                                                                 <  1:57046/170‑1 (MQ=255)                                                                                             aaataaaaaaacaaccgcggaaaGCACGAAAAACGGAAAAATGCTGC‑GGCCGGGAAAAGAAGATGTTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCGAAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGaac                                                                                                                                                 <  1:849345/230‑1 (MQ=255)                                                                                             aaaaaaaaaaGACAGCGCCGAAAGCACGAAAAACGGCAAAATGCTGCTGACGTTG‑AAAGAAGAGGGTAACGGGGGGGTAAACATGGGCGAACAGTTTTGGGAGCCAAAAAAAATT‑CCT‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGaac                                                                                                                                                 <  1:781099/247‑1 (MQ=255)                                                                                               aTAAAAAAGACATTCCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGACCCGATTTGGGAGCCGAATAAAATT‑CCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTggagga                                                                                                                                                                        <  1:245067/226‑1 (MQ=255)                                                                                                        ccATCGCCGTAAGCACGCAAACGGGCAAAATGCTGCTGACCGTA‑AAAAAAGAGGGTAACGTGGGGGTAAACATGGGAGACCCGTTTTGGGAGCCGAA‑AAAATTCCCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTcc                                                                                                                                       <  1:158792/249‑1 (MQ=255)                                                                                                            cGCCGTAAGCACGCAAAAGGGAAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCGGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAAc                                                                                                                                                               <  1:26460/222‑1 (MQ=255)                                                                                                            cGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAAc                                                                                                                                                                                                                                 <  1:562621/156‑1 (MQ=255)                                                                                                            cGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTAt                                                                                                                                  >  1:549832/1‑251 (MQ=255)                                                                                                             gCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      >  1:898169/1‑182 (MQ=255)                                                                                                             gCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTAtt                                                                                                                                 >  1:756257/1‑251 (MQ=255)                                                                                                                 aaagcccgaaaaaCGGAAAAATGCTGCTGCCGGTG‑AAAGAAGATGGTAACGGGGGGGTAAACAGGGGCGAACCGTTTGGGGAGCCGAA‑AAAATTCCCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGaa                                                                                                                             <  1:173223/242‑1 (MQ=255)                                                                                                                    gCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGaac                                                                                                                                                 >  1:465211/1‑228 (MQ=255)                                                                                                                      acgaaaaaCGGAAAAATGTTGCTGACGGTA‑AAAGAAGATGGTACCGTGGGGGTAAACGGGGGAGAACCTTTTGGGGAGCGGAATAAATTC‑CCT‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCa                                                                                                                        <  1:103440/247‑1 (MQ=255)                                                                                                                                     aTGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGc                                                                                                                                                                                                     >  1:559663/1‑159 (MQ=255)                                                                                                                                     aTGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGa                                                                                                         >  1:722499/1‑251 (MQ=255)                                                                                                                                       gctgctGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      >  1:158722/1‑156 (MQ=255)                                                                                                                                        ctgctgACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATg                                                                                                                                                                                                        >  1:738395/1‑153 (MQ=255)                                                                                                                                               ccGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTGTCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAAt                                                                                               >  1:153352/1‑251 (MQ=255)                                                                                                                                                            aTGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTg                                                                                                                                                                             >  1:630065/1‑161 (MQ=255)                                                                                                                                                              ggtacggtgggggTAAACAGGGGCGAACCGATTTGGGAGCGAAAAAAAATT‑CCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGc                                                                                  <  1:473861/240‑1 (MQ=255)                                                                                                                                                              ggtaaggtggggggaaaCAGGGGGGAACCGTTTGGGGAGCCGAATAAATTC‑CCT‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGc                                                                                  <  1:57886/235‑1 (MQ=255)                                                                                                                                                                    gTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAggtggg                                                                           >  1:671619/1‑250 (MQ=255)                                                                                                                                                                       ggggTGAACAGGGGCGAACCGTTTGGGGAGCCAAAAAAAATT‑CCT‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGc                                                                                  <  1:627548/240‑1 (MQ=255)                                                                                                                                                                         ggtaaacaggggcaaacggtttggggAGCCAAAAAAAATC‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAAt                                                                       <  1:310377/227‑1 (MQ=255)                                                                                                                                                                           aaaacaggggaaacccgtttggggAGCAGAA‑AAAATT‑TCGTTTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGt                                                                    <  1:551934/230‑1 (MQ=255)                                                                                                                                                                            gAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGt                                                                    >  1:146192/1‑250 (MQ=255)                                                                                                                                                                                 tGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAAtg                                                                                                                                                       >  1:485309/1‑162 (MQ=255)                                                                                                                                                                                      aaaCCGTTTTGGGAGCCGAATAAATTC‑CCT‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCaa                                                         <  1:289566/250‑1 (MQ=255)                                                                                                                                                                                         tttatagaaaacagaaaTAAATTT‑CCT‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCTTTGCGTGGTGCAGGTGGAGAATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACt                                                      <  1:432494/236‑1 (MQ=255)                                                                                                                                                                                         cccgtttggggggccaaaaaaaAT‑CCG‑TTTTCCCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAAc                                                       <  1:327599/233‑1 (MQ=255)                                                                                                                                                                                           gATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTa                                                     >  1:871782/1‑250 (MQ=255)                                                                                                                                                                                              tggggAGCC‑AAAAAAATTCCCG‑TTTC‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTAcgcg                                                 <  1:434000/249‑1 (MQ=255)                                                                                                                                                                                                           aaaaTT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAAt                                                                       >  1:624558/1‑216 (MQ=255)                                                                                                                                                                                                              aTT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCTAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:799968/86‑1 (MQ=255)                                                                                                                                                                                                                    g‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATggg                                                                                                                                                                                                      <  1:748968/81‑1 (MQ=255)                                                                                                                                                                                                                                      tttGAGAAAAATTATATTCTGCGTACGGAAATCCAACCGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCgg            <  1:434816/251‑1 (MQ=255)                                                                                                                                                                                                                                             cagaTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAgcgg      <  1:194031/247‑1 (MQ=255)                                                                                                                                                                                                                                              aaaTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACt                                                                                                                                             <  1:274631/114‑1 (MQ=255)                                                                                                                                                                                                                                               aaTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAgcggcg    >  1:116144/1‑250 (MQ=255)                                                                                                                                                                                                                                                aTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTg                                                                                                                                                                             <  1:84397/80‑1 (MQ=255)                                                                                                                                                                                                                                                aTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTg                                                                                                  >  1:377411/1‑155 (MQ=255)                                                                                                                                                                                                                                                aTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAAc                                        <  1:46382/213‑1 (MQ=255)                                                                                                                                                                                                                                                aTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAgcggcg    >  1:839184/1‑249 (MQ=255)                                                                                                                                                                                                                                                aTTATATTCTGCGTACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAGCGGCGCt  <  1:730957/251‑1 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              ATCGCATTTTTAACGCGGACGGCAGCGAAGTGGCGCAATGTGGCAACGGCGCACGCTGTTTTGCCCGTTTCGTGACGCTCAAAGGCTTAACC‑AATAAAAAAGACATCGCCGTAAGCACGCAAAACGGCAAAATGCTGCTGACCGTG‑AAAGAAGATGGTAACGTGCGGGTGAACATGGGCGAACCGATTTGGGAGCCGAATAAAATT‑CCG‑TTTA‑CCGCCAATAAATTTGAGAAAAATTATATTCTGCGCACGGAAATCCAAACGGTGCTGTGCAGCGCGGTATCTATGGGCAATCCGCATTGCGTGGTGCAGGTGGAGGATATTCAAACCGCCAATGTGGAACAACTCGGTCCGCTATTGGAAAATCATGAACGTTTTCCGGAACGGGTGAATGCGGGCTTTATGCAGGTGGTGAATCGTCATCACATCAAACTACGCGTATACGAACGCGGTACCGGCGAAACCCAGGCTTGCGGTAGCGGCGCT  >  1/1670738‑1671222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑