Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,664,181 G→A A98A (GCG→GCA)  unknown → Aspartate‑semialdehyde dehydrogenase (EC 1.2.1.11)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,664,1810GA100.0% 217.8 / NA 79A98A (GCG→GCA) unknownAspartate‑semialdehyde dehydrogenase (EC 1.2.1.11)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/36);  total (43/36)

TTTTAATGGATCGTATGCAGCAGGAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCGGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAATTATTGGTG  >  1/1663934‑1664421
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
ttttAATGGATCGTATGCAGCAGGAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATgcagcg                                                                                                                                                                                                                                               <  1:201139/251‑1 (MQ=255)
         aTCGTATGCAGCAGGAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTg                                                                                                                                                                                                                                      >  1:188916/1‑251 (MQ=255)
                    cagGAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGga                                                                                                                                                                                                                              <  1:766969/248‑1 (MQ=255)
                       gAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCATTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGGATGga                                                                                                                                                                                                                              >  1:897577/1‑245 (MQ=255)
                                       atatCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTg                                                                                                                                                                                                        >  1:565010/1‑251 (MQ=255)
                                        tatCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTc                                                                                                                                                                                                                                             >  1:140180/1‑213 (MQ=255)
                                           cAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGAcc                                                                                                                                                                                                    <  1:499778/251‑1 (MQ=255)
                                                    cttcttTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATgcagcg                                                                                                                                                                                                                                               <  1:714730/199‑1 (MQ=255)
                                                       cttTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGgaag                                                                                                                                                                                                                            <  1:421826/215‑1 (MQ=255)
                                                       cttTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCaa                                                                                                                                                                                         >  1:709234/1‑250 (MQ=255)
                                                               cTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGAc                                                                                                                                                                                                     <  1:827323/230‑1 (MQ=255)
                                                               cTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGAc                                                                                                                                                                                                     >  1:343639/1‑230 (MQ=255)
                                                               cTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCa                                                                                                                                                                                          >  1:336516/1‑241 (MQ=255)
                                                                ttCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTAACGTGTTGGACCCGGTGAATCAACACGTGATCa                                                                                                                                                                               >  1:361426/1‑251 (MQ=255)
                                                                 tctcAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAg                                                                                                                                                                              <  1:822686/251‑1 (MQ=255)
                                                                        cAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAgg                                                                                                                                                                        <  1:365824/250‑1 (MQ=255)
                                                                          ggACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCg                                                                                                                                                                                                             >  1:417114/1‑211 (MQ=255)
                                                                          ggACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCt                                                                                                                                                                      >  1:390103/1‑250 (MQ=255)
                                                                              aaGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGaaa                                                                                                                                                                 <  1:252841/251‑1 (MQ=255)
                                                                              aaGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGaaa                                                                                                                                                                 <  1:171859/251‑1 (MQ=255)
                                                                                 cTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGAc                                                                                                                                                                                                     <  1:590692/212‑1 (MQ=255)
                                                                                              cggcggTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGaaa                                                                                                                                                                 <  1:623615/235‑1 (MQ=255)
                                                                                                   gTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTa                                                                                                                                            >  1:109242/1‑251 (MQ=255)
                                                                                                            ccGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAac                                                                                                                                   <  1:490618/251‑1 (MQ=255)
                                                                                                            ccGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATG‑AGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAac                                                                                                                                   <  1:805093/250‑1 (MQ=255)
                                                                                                                 ccattGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGGCGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtac                                                                                                                              <  1:457024/248‑1 (MQ=255)
                                                                                                                 aCCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtac                                                                                                                              <  1:393380/251‑1 (MQ=255)
                                                                                                                   cTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgta                                                                                                                               >  1:279664/1‑248 (MQ=255)
                                                                                                                    ttGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtactgt                                                                                                                           <  1:351155/251‑1 (MQ=255)
                                                                                                                    ttGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtactgt                                                                                                                           <  1:804265/251‑1 (MQ=255)
                                                                                                                    ttGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtactgt                                                                                                                           <  1:434809/251‑1 (MQ=255)
                                                                                                                      gAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCa                                                                                                                         >  1:7597/1‑251 (MQ=255)
                                                                                                                          aaTGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAAc                                                                                                                                                              >  1:57721/1‑210 (MQ=255)
                                                                                                                           aTGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTg                                                                                                                    <  1:559141/251‑1 (MQ=255)
                                                                                                                             gCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGaaa                                                                                                                                                                 <  1:701218/204‑1 (MQ=255)
                                                                                                                             gCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtac                                                                                                                              >  1:618395/1‑239 (MQ=255)
                                                                                                                                ttCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGc                                                                                                                <  1:219839/250‑1 (MQ=255)
                                                                                                                                       ttGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCg                                                                                                        >  1:124090/1‑251 (MQ=255)
                                                                                                                                              aCTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACCGTCAGCTTGATGCTCATAGCg                                                                                                        >  1:873914/1‑244 (MQ=255)
                                                                                                                                               cTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtactgt                                                                                                                           >  1:480437/1‑224 (MQ=255)
                                                                                                                                                  aaaaaaCTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGttt                                                                                             <  1:709318/251‑1 (MQ=255)
                                                                                                                                                     aaaCTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATc                                                                                                                                                                                <  1:690001/165‑1 (MQ=255)
                                                                                                                                                      aaCTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCg                                                                                                                                                                                                             >  1:568474/1‑135 (MQ=255)
                                                                                                                                                      aaCTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtac                                                                                                                              >  1:319015/1‑214 (MQ=255)
                                                                                                                                                          ggACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGc                                                                                                  <  1:792536/238‑1 (MQ=255)
                                                                                                                                                          ggACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGaaa                                                                                     >  1:716936/1‑251 (MQ=255)
                                                                                                                                                               ttATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGAt                                                                                >  1:189185/1‑251 (MQ=255)
                                                                                                                                                                 aTCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCg                                                                                                        >  1:344296/1‑225 (MQ=255)
                                                                                                                                                                 aTCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGAttt                                                                              >  1:777413/1‑251 (MQ=255)
                                                                                                                                                                     tCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGAcc                                                                                                                                                                                                    >  1:898007/1‑129 (MQ=255)
                                                                                                                                                                     tCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATc                                                                                                                                                                                <  1:221284/149‑1 (MQ=255)
                                                                                                                                                                     tCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAg                                                                                                                                                                              >  1:604309/1‑151 (MQ=255)
                                                                                                                                                                        ccTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGaa                                                                       <  1:828773/251‑1 (MQ=255)
                                                                                                                                                                            ccAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATgg                                                                    <  1:114375/250‑1 (MQ=255)
                                                                                                                                                                            ccAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATgg                                                                    <  1:317730/250‑1 (MQ=255)
                                                                                                                                                                              aaGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGAtc                                                                 <  1:77882/251‑1 (MQ=255)
                                                                                                                                                                                    gcgATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGt                                                                                                                                             >  1:683715/1‑169 (MQ=255)
                                                                                                                                                                                     cgATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTg                                                          >  1:374991/1‑251 (MQ=255)
                                                                                                                                                                                      gATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAg                                                                                                                                           >  1:657863/1‑169 (MQ=255)
                                                                                                                                                                                      gATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTg                                                          >  1:298502/1‑250 (MQ=255)
                                                                                                                                                                                      gATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTg                                                          >  1:56517/1‑250 (MQ=255)
                                                                                                                                                                                       aTTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGCGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTTATGCTCATAGCGTTAGGc                                                                                                  >  1:397545/1‑209 (MQ=255)
                                                                                                                                                                                                   aaGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAgc                                             <  1:292002/250‑1 (MQ=255)
                                                                                                                                                                                                         aTCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAgt                                      <  1:754695/251‑1 (MQ=255)
                                                                                                                                                                                                          tCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTg                                                                                        >  1:445860/1‑200 (MQ=255)
                                                                                                                                                                                                                 ttAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCgg                              <  1:516781/251‑1 (MQ=255)
                                                                                                                                                                                                                       gCCACCGGCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGaa                        >  1:851682/1‑251 (MQ=255)
                                                                                                                                                                                                                             ggCTGGAGCGGTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAatat                  >  1:669092/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGa                                                                                                                                                                           <  1:265476/88‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTATGCGGTAACCGTACTGTCAGCGTGATGCTCATATCGTTAGGCGGCTTATTTGAAAAAGTTTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGaagta        >  1:422750/1‑248 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAactgtac                                                                                                                              <  1:267976/133‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGc                                                                                                  >  1:289349/1‑161 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAgc                                             >  1:741839/1‑214 (MQ=255)
                                                                                                                                                                                                                                       gTTACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAatta        >  1:247491/1‑251 (MQ=255)
                                                                                                                                                                                                                                         tACTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAac                                                                                                                                   <  1:340955/126‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGAt                                                                  >  1:349673/1‑189 (MQ=255)
                                                                                                                                                                                                                                           cTGGGTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAATTATTgg    <  1:489095/251‑1 (MQ=255)
                                                                                                                                                                                                                                              ggTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGc                                                                                                  >  1:31485/1‑154 (MQ=255)
                                                                                                                                                                                                                                              ggTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAATTATTGGTg  >  1:798981/1‑250 (MQ=255)
                                                                                                                                                                                                                                              ggTGGATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAATTATTGGTg  <  1:385690/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  gATGCAGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCgg                              >  1:528473/1‑218 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
TTTTAATGGATCGTATGCAGCAGGAAAATGATTTTACGAATATCAATCCGATCTTCTTTACCACTTCTCAAGCAGGACAAGCTGCACCGACATTCGGCGGTAAAGACGCCGGCACCTTGAAAAATGCGTTCGACATTGAAGAACTGAAAAAACTGGACATTATCGTCACCTGCCAAGGCGGCGATTACACCAACGAAGTTTATCCGAAATTAAAAGCCACCGGCTGGAGCGGTTACTGGGTGGATGCGGCGTCCGCATTGCGTATGGAAGAAGACGCGATTATCGTGTTGGACCCGGTGAATCAACACGTGATCAGCGAAGGCTTGAAAAACGGCGTGAAAACCTATGTAGGCGGTAACTGTACTGTCAGCTTGATGCTCATAGCGTTAGGCGGTTTATTTGAAAAAGATTTAGTGGAATGGATCTCCGTTGCCACTTACCAAGCCGCCAGTGGTGCCGGTGCGAAAAATATGCGCGAATTATTGGTG  >  1/1663934‑1664421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑