Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,661,952	C→T	intergenic (‑9/+108)	unknown ← / ← unknown	[4Fe‑4S] cluster carrier protein NfuA/Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,661,952	0	C	T	100.0%	127.9 / NA	48	intergenic (‑9/+108)	unknown/unknown	[4Fe‑4S] cluster carrier protein NfuA/Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/29);  total (19/29)

ATTTCCGCATCTTCCAGAAACGGCAGGCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGACTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCCACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTAACGCCACGCTACGATAAGGAAAATGT  >  1/1661705‑1662192                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aTTTCCGCATCTTCCAGAAACGGCAGGCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCtaca                                                                                                                                                                                                                                               >  1:504657/1‑251 (MQ=255)  tttCCGCATCTTCCAGAAACGGCAGGCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACaa                                                                                                                                                                                                                                              <  1:241811/251‑1 (MQ=255)                   aaCGGCAGGCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAAtt                                                                                                                                                                                                                             <  1:858716/251‑1 (MQ=255)                           gCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCgg                                                                                                                                                                                                                       <  1:705569/249‑1 (MQ=255)                                   cGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACg                                                                                                                                                                                                             >  1:5407/1‑251 (MQ=255)                                     tCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGcc                                                                                                                                                                                                           <  1:134867/251‑1 (MQ=255)                                                 gagaAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGtttt                                                                                                                                                                                               >  1:790803/1‑251 (MQ=255)                                                    aaaCTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGtttttt                                                                                                                                                                                             <  1:715497/250‑1 (MQ=255)                                                         gTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGAt                                                                                                                                                                                                                <  1:167884/226‑1 (MQ=255)                                                         gTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTa                                                                                                                                                                                        >  1:556632/1‑250 (MQ=255)                                                                           gtcgtcGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTAc                                                                                                                                                                                <  1:276236/240‑1 (MQ=255)                                                                                                  gTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCcttttc                                                                                                                                                    <  1:144035/245‑1 (MQ=255)                                                                                                     ggCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAgg                                                                                                                                            <  1:748637/250‑1 (MQ=255)                                                                                                      gCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATa                                                                                                                                                                                                               >  1:583049/1‑182 (MQ=255)                                                                                                               aaCGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCATGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGt                                                                                                                                 <  1:179338/251‑1 (MQ=255)                                                                                                               aaCGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGt                                                                                                                                 <  1:240774/251‑1 (MQ=255)                                                                                                                 cGCCACATTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTAc                                                                                                                               <  1:556972/251‑1 (MQ=255)                                                                                                                     acaTTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATAc                                                                                                                                                                                                              >  1:397423/1‑168 (MQ=255)                                                                                                                     acaTTCGGCATTCGGCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGc                                                                                                                                                                                                            <  1:168526/170‑1 (MQ=255)                                                                                                                      cattcggcattcggCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCa                                                                                                                          <  1:308384/251‑1 (MQ=255)                                                                                                                      cattcggcattcggCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCa                                                                                                                          <  1:442788/251‑1 (MQ=255)                                                                                                                      cattcggcattcggCGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCa                                                                                                                          <  1:196848/251‑1 (MQ=255)                                                                                                                        ttcggcattcggcGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAg                                                                                                                        >  1:842373/1‑251 (MQ=255)                                                                                                                                   gcGTGCCGGGATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACAcc                                                                                                              <  1:460267/250‑1 (MQ=255)                                                                                                                                            gATTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAggg                                                                                                                                           <  1:234697/212‑1 (MQ=255)                                                                                                                                             aTTGGCTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTAc                                                                                                   <  1:793545/251‑1 (MQ=255)                                                                                                                                                ggcTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATAc                                                                                                                                                                                                              <  1:165072/139‑1 (MQ=255)                                                                                                                                                ggcTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCa                                                                                                 >  1:901583/3‑250 (MQ=255)                                                                                                                                                              gAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTAc                                                                                                                                                                                >  1:176851/1‑157 (MQ=255)                                                                                                                                                                    gAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAatat                                                                                                       >  1:155957/1‑224 (MQ=255)                                                                                                                                                                    gAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAatat                                                                                                       >  1:276511/1‑224 (MQ=255)                                                                                                                                                                    gAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCg                                                                              >  1:792034/1‑249 (MQ=255)                                                                                                                                                                         ccTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAatat                                                                                                       <  1:218393/219‑1 (MQ=255)                                                                                                                                                                            tcttcCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGAtt                                                                    >  1:172386/1‑251 (MQ=255)                                                                                                                                                                             cttcCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTAc                                                                                                                               <  1:369117/191‑1 (MQ=255)                                                                                                                                                                                 cTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCG‑TTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGttt                                                              >  1:365909/1‑251 (MQ=255)                                                                                                                                                                                       aTCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAgg                                                          <  1:540395/250‑1 (MQ=255)                                                                                                                                                                                           aaTAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAggg                                                                                                                                           <  1:15344/165‑1 (MQ=255)                                                                                                                                                                                                   gCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTa                                                                                                                                >  1:2230/1‑168 (MQ=255)                                                                                                                                                                                                   gCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCCTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCggtgg                                              <  1:457505/250‑1 (MQ=255)                                                                                                                                                                                                      aaaaTGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTCCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGAt                                          >  1:710853/1‑251 (MQ=255)                                                                                                                                                                                                         aTGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATcac                                       <  1:103108/251‑1 (MQ=255)                                                                                                                                                                                                                             aaaTAGTAATTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTAACGCCACg                    >  1:220006/1‑250 (MQ=255)                                                                                                                                                                                                                                    aaTTTGTTGCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTaa                           <  1:637314/236‑1 (MQ=255)                                                                                                                                                                                                                                       ttgttgCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAggg                                                                                                                                           >  1:550252/1‑121 (MQ=255)                                                                                                                                                                                                                                            gCATAACTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATcaca                                      <  1:260447/217‑1 (MQ=255)                                                                                                                                                                                                                                                 aCTCTCCTACAATAAAATCAGATTAAATTTGTCGGATATGATAc                                                                                                                                                                                                              <  1:494842/44‑1 (MQ=255)                                                                                                                                                                                                                                                  ctctCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTaa                           >  1:476819/1‑222 (MQ=255)                                                                                                                                                                                                                                                  ctctCCTACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTAACGCCACGCTACGATAAGGAAAAtgt  >  1:624062/1‑247 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ATTTCCGCATCTTCCAGAAACGGCAGGCTGATTTCGTCTACAAAGGCGGAGAAACTGTCGTATTTGATTTCCATGTCGTCGGCTTCAACGGAATTCGGTGGGCAGTAAGAAACGCCACATTCGGCATTCGGCGTGCCGGGATTGACTACGAAAATACGAATATGAGTGCCTTCTTCCTGTTGATCCAATAAACGGCGAAAATGCGCTTGCGCCGCTTCTGAAATAGTAATTTGTTGCATAACTCTCCCACAATAAAATCAGATTAAATTTGTCGGATATGATACGCCTCACGCCGTTTTTTTTCTACCTTTTACTATAATAAAAAAGCGGATTAGCCTTTTCTTTTAAGGGAGCGTTATGTACGGGCAAGCCCCCACACCTGAATATGTACCACGTTGGCTTGGCGTAACCGCTTGGCGATTTCGTTTAAGGTGGAGCCGGTGGTGATCACATCGTCCACTAACGCCACGCTACGATAAGGAAAATGT  >  1/1661705‑1662192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑