Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,619,091	G→A	E218E (GAG→GAA)	unknown →	DNA recombination and repair protein RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,619,091	0	G	A	100.0%	220.5 / NA	79	E218E (GAG→GAA)	unknown	DNA recombination and repair protein RecO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/38);  total (41/38)

GCATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAGTTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGCACAGCGC  >  1/1618847‑1619329                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gCATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTAttt                                                                                                                                                                                                                                           >  1:493677/1‑251 (MQ=255) gCATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTAttt                                                                                                                                                                                                                                           >  1:556865/1‑251 (MQ=255) gCATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTAttt                                                                                                                                                                                                                                           >  1:615703/1‑251 (MQ=255)  cATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTAttt                                                                                                                                                                                                                                           <  1:69883/250‑1 (MQ=255)       aTTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTAcaca                                                                                                                                                                                                                                      <  1:677972/250‑1 (MQ=255)        tttCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACacaa                                                                                                                                                                                                                                     >  1:305267/1‑250 (MQ=255)             cccATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGc                                                                                                                                                                                                                               <  1:842312/251‑1 (MQ=255)              ccATTGCGCCAGTTCGGACAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCt                                                                                                                                                                                                                              <  1:542437/251‑1 (MQ=255)               cATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCtt                                                                                                                                                                                                                             <  1:325273/251‑1 (MQ=255)               cATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCtt                                                                                                                                                                                                                             <  1:677512/251‑1 (MQ=255)                   gcgcCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTatat                                                                                                                                                                                                                         >  1:114655/1‑251 (MQ=255)                   gcgcCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTatat                                                                                                                                                                                                                         >  1:157236/1‑251 (MQ=255)                      ccAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTc                                                                                                                                                                                                                       <  1:809339/250‑1 (MQ=255)                      ccAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTc                                                                                                                                                                                                                       <  1:324325/250‑1 (MQ=255)                          ttCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAAt                                                                                                                                                                                                                  <  1:68438/251‑1 (MQ=255)                                  acacGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATgg                                                                                                                                                                                                           >  1:217066/1‑250 (MQ=255)                                     cGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCAtt                                                                                                                                                                                                       >  1:197876/1‑251 (MQ=255)                                     cGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCAtt                                                                                                                                                                                                       >  1:667038/1‑251 (MQ=255)                                     cGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCAtt                                                                                                                                                                                                       >  1:528855/1‑251 (MQ=255)                                     cGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCAtt                                                                                                                                                                                                       >  1:37317/1‑251 (MQ=255)                                     cGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAAAATGGCAtt                                                                                                                                                                                                       >  1:770556/1‑251 (MQ=255)                                        tAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTAc                                                                                                                                                                                                     <  1:511374/250‑1 (MQ=255)                                           aaGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTAttt                                                                                                                                                                                                                                           <  1:358536/209‑1 (MQ=255)                                                gACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAAt                                                                                                                                                                                                                  <  1:498190/229‑1 (MQ=255)                                                gACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCg                                                                                                                                                                                            >  1:216643/1‑251 (MQ=255)                                                 aCATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAAc                                                                                                                                                                                                               >  1:601402/1‑231 (MQ=255)                                                    tGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAAc                                                                                                                                                                                                               <  1:429307/228‑1 (MQ=255)                                                     gACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCtt                                                                                                                                                                                                                             <  1:522825/213‑1 (MQ=255)                                                     gACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAAt                                                                                                                                                                                                                  >  1:788741/1‑224 (MQ=255)                                                     gACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACAt                                                                                                                                                                                                             <  1:99128/229‑1 (MQ=255)                                                     gACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAAc                                                                                                                                                                                        >  1:643326/1‑250 (MQ=255)                                                     gACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTAACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAAc                                                                                                                                                                                        >  1:447048/1‑250 (MQ=255)                                                         tATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTAc                                                                                                                                                                                                     <  1:594324/233‑1 (MQ=255)                                                                      aagaaAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATAc                                                                                                                                                                     <  1:636273/251‑1 (MQ=255)                                                                              ggCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGAATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAAtt                                                                                                                                                             >  1:756240/1‑251 (MQ=255)                                                                                  ttATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCAc                                                                                                                                                         <  1:584790/251‑1 (MQ=255)                                                                                                 ttaaAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGa                                                                                                                                          <  1:352605/251‑1 (MQ=255)                                                                                                    aaaaCAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGTTTTGGCGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGGGAATTATTTGCACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAGCCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGAttt                                                                                                                                       <  1:895489/248‑1 (MQ=255)                                                                                                     aGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTatat                                                                                                                                                                                                                         <  1:311449/169‑1 (MQ=255)                                                                                                                cTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGcccccgtctcctatacacatctccgag                                                                                                                            >  1:180958/1‑226 (MQ=255)                                                                                                                  ttttACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGc                                                                                                                         <  1:379797/251‑1 (MQ=255)                                                                                                                                   gCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCGAAAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGAt                                                                                                                                   >  1:602385/1‑225 (MQ=255)                                                                                                                                              aGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATcc                                                                                                                                              <  1:570825/202‑1 (MQ=255)                                                                                                                                                  aaCCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCg                                                                                                                   >  1:483037/1‑225 (MQ=255)                                                                                                                                                    cccGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGtt                                                                                       <  1:805335/251‑1 (MQ=255)                                                                                                                                                                 cccGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATgcg                                                                          >  1:2982/1‑251 (MQ=255)                                                                                                                                                                    gAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTa                                                                       >  1:253139/1‑251 (MQ=255)                                                                                                                                                                      gCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTAc                                                                      >  1:633840/1‑250 (MQ=255)                                                                                                                                                                         tatTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCa                                                                  >  1:6829/1‑251 (MQ=255)                                                                                                                                                                         tatTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCCCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCa                                                                  >  1:131395/1‑251 (MQ=255)                                                                                                                                                                         tatTAGTGGCGGCAAAACGGGTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCa                                                                  >  1:476492/1‑251 (MQ=255)                                                                                                                                                                          atTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAg                                                                 >  1:250841/1‑251 (MQ=255)                                                                                                                                                                           ttAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAgg                                                                <  1:858000/251‑1 (MQ=255)                                                                                                                                                                            tAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGa                                                               <  1:388338/251‑1 (MQ=255)                                                                                                                                                                                  cggcAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGt                                                         <  1:784470/251‑1 (MQ=255)                                                                                                                                                                                    gcAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGa                                                                                                                                    >  1:873948/1‑174 (MQ=255)                                                                                                                                                                                     aaaaaCGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGa                                                      <  1:202916/250‑1 (MQ=255)                                                                                                                                                                                       aaaCGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAg                                                    <  1:165358/251‑1 (MQ=255)                                                                                                                                                                                         aCGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTAc                                                                                                                                                                                                <  1:675631/110‑1 (MQ=255)                                                                                                                                                                                         aCGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGAtt                                                                                                                                        >  1:431882/1‑165 (MQ=255)                                                                                                                                                                                           gTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCg                                                >  1:225955/1‑251 (MQ=255)                                                                                                                                                                                           gTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCg                                                >  1:599047/1‑251 (MQ=255)                                                                                                                                                                                            ttttACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCg                                                <  1:46434/250‑1 (MQ=255)                                                                                                                                                                                                       tCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTAc                                                                                                                                                                                                     >  1:552909/1‑91 (MQ=255)                                                                                                                                                                                                         gCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGaaa                                  >  1:851932/1‑251 (MQ=255)                                                                                                                                                                                                          ccTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTAc                                                                                                                                                                                                >  1:260963/1‑93 (MQ=255)                                                                                                                                                                                                                     tATTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACg                      >  1:369369/1‑251 (MQ=255)                                                                                                                                                                                                                      aTTTAGGTGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACgg                     >  1:176442/1‑251 (MQ=255)                                                                                                                                                                                                                             tGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACgg               >  1:75617/1‑250 (MQ=255)                                                                                                                                                                                                                             tGACAAGCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGc              <  1:630488/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCCATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGc                                                                   >  1:321523/1‑192 (MQ=255)                                                                                                                                                                                                                                    ccATTGAAAGGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCA‑AAAAAAAATGCCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGcac       <  1:434837/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGcac       <  1:393621/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGcac       <  1:515536/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGcac       <  1:612596/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGcac       <  1:658884/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttGAAAAGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGa                                                      >  1:335794/1‑201 (MQ=255)                                                                                                                                                                                                                                          aaaaGCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGCACAgcgc  <  1:220810/250‑1 (MQ=255)                                                                                                                                                                                                                                              gCCGAGAATTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑‑AAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAg                                                    >  1:388093/1‑195 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GCATTGATTTCACCCATTGCGCCAGTTCGGGCAACACGGTAGAAGCGGACATGACCTATCGTTACCGCGAAGAAAAAGGCTTTATCGCCTCCTTAATTAAAGACAATTTAACTTTTTACGGGCGCGAATTGCTGGCATTTGAGCAAACCCGATTTGACGACCCGAGCATATTAGTGGCGGCAAAACGTTTTACACGCATCGCCTTAAAGCCTTATTTAGGTGACAAGCCATTGAAAAGCCGAGAGTTATTTACACAACATAGCTTATATTCCAAATAACATGGCATTACTTTACAGCGGAACCAAACCG‑AAAAAAAATACCGCCAATTTCACCGCCCACATCCTTGATTTGGATTATCAGGGGCTGGGCGTAGCGAAAATGAACGGCAAAACCTGGTTTATCGAAAATGCGCTACCGCAGGAAAGCGTGGAAGTGCGTGTGCTGGAAGAAAAACGCCAATACGGACACGGCATAGCACAGCGC  >  1/1618847‑1619329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑