Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,618,282	G→A	intergenic (+141/‑156)	unknown → / → unknown	Translation elongation factor Ts/DNA recombination and repair protein RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,618,282	0	G	A	100.0%	165.3 / NA	61	intergenic (+141/‑156)	unknown/unknown	Translation elongation factor Ts/DNA recombination and repair protein RecO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/23);  total (38/23)

GTCTCTAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCGTCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTGGCAAAAGG  >  1/1618049‑1618526                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     gTCTCTAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACAt                                                                                                                                                                                                                                     >  1:652202/1‑251 (MQ=255) gTCTCTAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACAt                                                                                                                                                                                                                                     >  1:644739/1‑251 (MQ=255)  tctcTAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACa                                                                                                                                                                                                                                      <  1:246960/249‑1 (MQ=255)   ctctAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACa                                                                                                                                                                                                                                      <  1:663110/248‑1 (MQ=255)   ctctAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACAttt                                                                                                                                                                                                                                   >  1:11357/1‑251 (MQ=255)               ccGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAAccc                                                                                                                                                                                                                       <  1:452165/251‑1 (MQ=255)                 gTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCAt                                                                                                                                                                                                                     >  1:477999/1‑251 (MQ=255)                 gTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCAt                                                                                                                                                                                                                     >  1:420367/1‑251 (MQ=255)                 gTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCAt                                                                                                                                                                                                                     >  1:290868/1‑251 (MQ=255)                 gTTTAGAAGTGGGCGAAGGCATCGAGAAAGAGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCAt                                                                                                                                                                                                                     >  1:622243/1‑251 (MQ=255)                       aaGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACA‑TTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCt                                                                                                                                                                                                                <  1:20831/249‑1 (MQ=255)                          tGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCttt                                                                                                                                                                                                              <  1:24564/249‑1 (MQ=255)                                aaGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAAt                                                                                                                                                                                                                                                 >  1:808390/1‑208 (MQ=255)                                  ggTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAAcc                                                                                                                                                                                                                        >  1:841794/1‑231 (MQ=255)                                     aTCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATcc                                                                                                                                                                                                 <  1:678078/251‑1 (MQ=255)                                             aGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCaaa                                                                                                                                                                                         >  1:215667/1‑251 (MQ=255)                                              gTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACa                                                                                                                                                                                                                                      >  1:115382/1‑205 (MQ=255)                                                  aaaCCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATcc                                                                                                                                                                                    <  1:143279/251‑1 (MQ=255)                                                  aaaCCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATc                                                                                                                                                                                     >  1:789001/1‑250 (MQ=255)                                                                       tGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAAt                                                                                                                                                                <  1:29049/250‑1 (MQ=255)                                                                         gCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAAt                                                                                                                                                                >  1:354548/1‑248 (MQ=255)                                                                            gCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTa                                                                                                                                                          >  1:128581/1‑251 (MQ=255)                                                                               aTGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAAt                                                                                                                                                                                      <  1:632071/220‑1 (MQ=255)                                                                                tGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAAt                                                                                                                                                      <  1:901004/251‑1 (MQ=255)                                                                                 gTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATcc                                                                                                                                                                                                 >  1:870072/1‑207 (MQ=255)                                                                                 gTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGagta                                                                                                                                                     >  1:3177/1‑248 (MQ=255)                                                                                 gTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATa                                                                                                                                                     >  1:513053/1‑251 (MQ=255)                                                                                 gTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATa                                                                                                                                                     >  1:720752/1‑251 (MQ=255)                                                                                  tCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATa                                                                                                                                                     <  1:87799/250‑1 (MQ=255)                                                                                     aaaGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAgcg                                                                                                                                                 <  1:851812/251‑1 (MQ=255)                                                                                        gCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCGCGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGc                                                                                                                                                                          <  1:236627/223‑1 (MQ=255)                                                                                                                                     tttttATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAAcg                                                                                                  >  1:7518/1‑250 (MQ=255)                                                                                                                                         gtcggtttccactttCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAg                                                                                                                                                                             >  1:95178/13‑171 (MQ=255)                                                                                                                                             ctcCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATcc                                                                                                                                                                                                 >  1:658814/1‑147 (MQ=255)                                                                                                                                                  gCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTGTAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTa                                                                                     >  1:38998/1‑250 (MQ=255)                                                                                                                                                            aTTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAAcc                                                                                                                                                                                                                        >  1:732384/1‑109 (MQ=255)                                                                                                                                                            aTTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATc                                                                          <  1:547332/251‑1 (MQ=255)                                                                                                                                                            aTTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAACTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATc                                                                          <  1:466837/251‑1 (MQ=255)                                                                                                                                                             ttCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAAt                                                                           >  1:583815/1‑249 (MQ=255)                                                                                                                                                                             aTAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGc                                                          <  1:771807/250‑1 (MQ=255)                                                                                                                                                                                gTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTc                                                                                                                                                                                                         >  1:85937/1‑104 (MQ=255)                                                                                                                                                                                  tCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTg                                                    <  1:144768/251‑1 (MQ=255)                                                                                                                                                                                             cGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGc                                                                                       >  1:531231/1‑205 (MQ=255)                                                                                                                                                                                             cGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTAc                                                      <  1:785986/238‑1 (MQ=255)                                                                                                                                                                                             cGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGa                                                   <  1:770901/241‑1 (MQ=255)                                                                                                                                                                                             cGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTa                                           >  1:102349/1‑249 (MQ=255)                                                                                                                                                                                             cGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTa                                           >  1:823200/1‑249 (MQ=255)                                                                                                                                                                                               cTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTc                                        <  1:367961/250‑1 (MQ=255)                                                                                                                                                                                                           aaaTAGCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACt                              >  1:99558/1‑248 (MQ=255)                                                                                                                                                                                                                gCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGttt                                                                                            >  1:707663/1‑181 (MQ=255)                                                                                                                                                                                                                gCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCgg                      >  1:831225/1‑251 (MQ=255)                                                                                                                                                                                                                gCACAGACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCgg                      >  1:749077/1‑251 (MQ=255)                                                                                                                                                                                                                     gACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATa                                                                                                                                                     >  1:837471/1‑119 (MQ=255)                                                                                                                                                                                                                     gACCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAg                 >  1:373733/1‑251 (MQ=255)                                                                                                                                                                                                                      aCCTGAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATc                   <  1:805705/248‑1 (MQ=255)                                                                                                                                                                                                                          gAACATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGt              >  1:538942/1‑249 (MQ=255)                                                                                                                                                                                                                             cATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATc                                                                          >  1:412794/1‑186 (MQ=255)                                                                                                                                                                                                                             cATTTACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTgg         >  1:165130/1‑251 (MQ=255)                                                                                                                                                                                                                                ttACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTGGCaa      >  1:852818/1‑251 (MQ=255)                                                                                                                                                                                                                                ttACACTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTGGCaa      <  1:186185/251‑1 (MQ=255)                                                                                                                                                                                                                                    acTACCATCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTGGCAAAAgg  <  1:163310/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     GTCTCTAACTTCATCCGTTTAGAAGTGGGCGAAGGTATCGAGAAAGTGGAAACCGACTTTGCCGCAGAAGTGGCTGCAATGTCTAAAGCTTAATTGATGATTTAAGCAAAAAAGCAGGTGTCAAAACCTGCTTTTTTATTCTCCGGCATTTCTCGATTCTCACGCTTGCCCGATAGTTCAAGCGTCCACGCTTAGACCGATAAAATAGCACAGACCTGAACATTTACACTACCGTCAATCAGTGCCGACATTTCCGTTATAACCCATTCCCTTTTATTCTAAAATCCCACGCAAAAATCCCACAAAGTGCGGTGTGAAATCTGCTAGAATAAGCGCAATTTTTTATCGGCGAAAAGGGCGACATCATCGAAAATTGGCAACGCGGTTTCGTGCTACATCGCAAATCTTACAGCGAAACCAGCTTACTGATCGATTTACTCACGGAAGACTTCGGGCGGATCAGCGTCTTGGCAAAAGG  >  1/1618049‑1618526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑