Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	306,732	G→A	L35L (CTG→TTG)	unknown ←	Oligopeptide ABC transporter, permease protein OppC (TC 3.A.1.5.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	306,732	0	G	A	100.0%	174.7 / NA	65	L35L (CTG→TTG)	unknown	Oligopeptide ABC transporter, permease protein OppC (TC 3.A.1.5.1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/38);  total (27/38)

CGCCCGCCGATGGCGGTACGCACCAAAAGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAGGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGAATAATCGCGGTTAAG  >  1/306500‑306963                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        cccccgccGAGGGCGGTACGCACCAAAAGAGCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTg                                                                                                                                                                                                                       <  1:458616/249‑1 (MQ=255)          aTGGCGGTACGCACCAAAAGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATc                                                                                                                                                                                                              <  1:325899/251‑1 (MQ=255)          aTGGCGGTACGCACCAAAAGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATc                                                                                                                                                                                                              <  1:262134/251‑1 (MQ=255)                       ccAAAAGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGt                                                                                                                                                                                                       >  1:580704/1‑245 (MQ=255)                         aaaaGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAg                                                                                                                                                                                               <  1:240695/251‑1 (MQ=255)                                     ccGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTcaacaa                                                                                                                                                                                    <  1:783825/250‑1 (MQ=255)                                                 gTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAATTTCGGCAttttt                                                                                                                                                                       >  1:752613/1‑251 (MQ=255)                                                      gaagaaATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCAt                                                                                                                                                                                                                    <  1:735901/201‑1 (MQ=255)                                                           aaTGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTAtt                                                                                                                                                             >  1:828564/1‑251 (MQ=255)                                                           aaTGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTAtt                                                                                                                                                             >  1:174667/1‑251 (MQ=255)                                                             tGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTgg                                                                                                                                                           >  1:629308/1‑251 (MQ=255)                                                                     ttCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAgtt                                                                                                                                                                                             <  1:187920/209‑1 (MQ=255)                                                                      tCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACAcc                                                                                                                                                  <  1:50361/251‑1 (MQ=255)                                                                                          catcatGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTa                                                                                                                               >  1:130435/1‑250 (MQ=255)                                                                                          catcatGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCC‑TCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTa                                                                                                                               >  1:101641/1‑249 (MQ=255)                                                                                           atcatGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACAcc                                                                                                                                                  <  1:717408/230‑1 (MQ=255)                                                                                           atcatGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTa                                                                                                                               >  1:442575/1‑249 (MQ=255)                                                                                            tcatGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACtt                                                                                                                            >  1:567810/1‑251 (MQ=255)                                                                                             catGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTc                                                                                                                           <  1:225320/251‑1 (MQ=255)                                                                                              atGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTgg                                                                                                                                                           <  1:352769/218‑1 (MQ=255)                                                                                              atGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTacac                                                                                                                                                   <  1:438325/226‑1 (MQ=255)                                                                                                gTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAg                                                                                                                                                                                               <  1:335936/180‑1 (MQ=255)                                                                                                          gtgtCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAAt                                                                                                              >  1:597461/1‑251 (MQ=255)                                                                                                            gtCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTAt                                                                                                                                                                           <  1:406581/188‑1 (MQ=255)                                                                                                            gtCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCAttttt                                                                                                                                         <  1:768229/222‑1 (MQ=255)                                                                                                              cTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAg                                                                                                                                                                                                                          >  1:582385/1‑139 (MQ=255)                                                                                                              cTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTacac                                                                                                                                                   <  1:692240/210‑1 (MQ=255)                                                                                                              cTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTtaataa                                                                                                                   >  1:807488/1‑242 (MQ=255)                                                                                                                      gTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGatcaat                                                                                                  >  1:741241/1‑251 (MQ=255)                                                                                                                      gTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACAATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGatcaat                                                                                                  >  1:786582/1‑251 (MQ=255)                                                                                                                            gggAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACgg                                                                                             >  1:457173/1‑250 (MQ=255)                                                                                                                             ggAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACgg                                                                                             <  1:356492/249‑1 (MQ=255)                                                                                                                                   cGAGGGGCGCAAAAGTAATGAAAAGCACGATAACCCCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATaaa                                                                                     <  1:593138/251‑1 (MQ=255)                                                                                                                                       gggCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAat                                                                                   <  1:230760/249‑1 (MQ=255)                                                                                                                                       gggCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAat                                                                                   <  1:129964/249‑1 (MQ=255)                                                                                                                                         gcgcAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGtt                                                                                                                                                                                                                        >  1:459950/1‑114 (MQ=255)                                                                                                                                         gcgcAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAat                                                                                   <  1:279985/247‑1 (MQ=255)                                                                                                                                         gcgcAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATc                                                                                >  1:215084/1‑250 (MQ=255)                                                                                                                                          cgcAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATcc                                                                               <  1:19431/250‑1 (MQ=255)                                                                                                                                            cAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAAATGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACt                                                                            <  1:572337/251‑1 (MQ=255)                                                                                                                                                    aTGAAAAGCACGATAACACCCAACATGATTAAACTGGCCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATc                                                                                                                                                                                                              <  1:275276/113‑1 (MQ=255)                                                                                                                                                           gCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAat                                                                                   <  1:282196/229‑1 (MQ=255)                                                                                                                                                            cACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAat                                                                                   <  1:106905/228‑1 (MQ=255)                                                                                                                                                                gtAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGatcaatc                                                                                                 >  1:884953/2‑210 (MQ=255)                                                                                                                                                                ataccacCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAg                                                         <  1:710093/246‑1 (MQ=255)                                                                                                                                                                aTAACACCCAACATGATTAAACTGCCCCCCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAg                                                         <  1:286272/250‑1 (MQ=255)                                                                                                                                                                aTAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGatcaatc                                                                                                 >  1:884970/1‑210 (MQ=255)                                                                                                                                                                        cAACATGATTAAACTGCCCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGGTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGc                                                <  1:433515/251‑1 (MQ=255)                                                                                                                                                                          aCATGATTAAACTGCCCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCg                                                                                           <  1:190950/206‑1 (MQ=255)                                                                                                                                                                             tGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTc                                                       >  1:186655/1‑239 (MQ=255)                                                                                                                                                                             tGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGAc                                            >  1:680074/1‑250 (MQ=255)                                                                                                                                                                              gATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTAtttttt                                                                                                                                                                      <  1:891212/127‑1 (MQ=255)                                                                                                                                                                                  aaaCTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTtaat                                                                                                                     >  1:19761/1‑172 (MQ=255)                                                                                                                                                                                  aaaCTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAAt                                      >  1:888914/1‑251 (MQ=255)                                                                                                                                                                                                  cTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATAc                       <  1:261834/250‑1 (MQ=255)                                                                                                                                                                                                  cTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATAc                       <  1:719654/250‑1 (MQ=255)                                                                                                                                                                                                    ttATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGCTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAg                    <  1:509846/251‑1 (MQ=255)                                                                                                                                                                                                      aTTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAggg                  <  1:126471/251‑1 (MQ=255)                                                                                                                                                                                                            gaagaaACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGaataat            >  1:290570/1‑251 (MQ=255)                                                                                                                                                                                                            gaagaaACGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGaataa             <  1:734662/250‑1 (MQ=255)                                                                                                                                                                                                                   cGCCGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTtaataa                                                                                                                   >  1:288169/1‑141 (MQ=255)                                                                                                                                                                                                                     ccGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACgg                                                                                             <  1:874507/161‑1 (MQ=255)                                                                                                                                                                                                                     ccGTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGAATAATCGCGGTTaa   >  1:899860/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTTGGCGTCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGAATAATCGCGGTTAAg  >  1:134955/1‑250 (MQ=255)                                                                                                                                                                                                                               tCTTGCCACAAGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACcatcat                                                                                                                                             <  1:252934/103‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        CGCCCGCCGATGGCGGTACGCACCAAAAGATCGCGGCCGGAGGAATCCGTACCGAAGAAATGGTAGCCTTCCAGCGTCGGCGCCACGCCCATCATGTTCCAGTCGGTGTCTTCATAAGTAAAAGGGAAAGCGAGGGGCGCAAAAGTAATGAAAAGCACGATAACACCCAACATGATTAAACTGACCACCGCCGCTTTATTGCGGAAGAAACGCCGTTTGGCGTCTTGCCACAGGCTGCGCCCTTCCAGTTGCATTTCATCCATGCGTTCCGCAAGTTGTTCAACAAATTCGGTATTTTTTTGATTTATTGGTTTTACACCATCATTTTTCGTCATGTTACTTCCTTAATAACGAATTTTCGGATCAATCACGGCGTATAAAATATCCACTACGGCGTTAAAAACGATAGTCAGTGTGCCGACCAAAATCGTGAGGCTCAATACAAGGGAATAATCGCGGTTAAG  >  1/306500‑306963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑