Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,602,310	T→C	L171S (TTA→TCA)	unknown →	Hypothetical lipoprotein YajG precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,602,310	0	T	C	100.0%	147.7 / NA	52	L171S (TTA→TCA)	unknown	Hypothetical lipoprotein YajG precursor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

CAACAGGATTTGGTCAGCAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTTAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGCTCCTCCTGCTGGACTTGAAC  >  1/1602063‑1602555                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      caacaGGATTTGGTCAGCAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAgg                                                                                                                                                                                                                                                    >  1:199758/1‑251 (MQ=255)                 gCAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGaa                                                                                                                                                                                                                                    >  1:97672/1‑251 (MQ=255)                  cAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      <  1:603463/248‑1 (MQ=255)                  cAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAg                                                                                                                                                                                                                                   <  1:422868/251‑1 (MQ=255)                  cAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAg                                                                                                                                                                                                                                   <  1:877174/251‑1 (MQ=255)                       gCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAgtggtg                                                                                                                                                                                                                              >  1:662677/1‑251 (MQ=255)                          tCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGaa                                                                                                                                                                                                                           >  1:242494/1‑251 (MQ=255)                              tATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCa                                                                                                                                                                                                                        <  1:587198/250‑1 (MQ=255)                                     aTGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATaa                                                                                                                                                                                                                <  1:200289/251‑1 (MQ=255)                                     aTGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATaa                                                                                                                                                                                                                <  1:687889/251‑1 (MQ=255)                                             cAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAgg                                                                                                                                                                                                        >  1:668625/1‑251 (MQ=255)                                              aaTTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGa                                                                                                                                                                                                       <  1:247304/251‑1 (MQ=255)                                                ttCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAg                                                                                                                                                                                                     <  1:167255/251‑1 (MQ=255)                                                       gctgcCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTaa                                                                                                                                                                                              >  1:585503/1‑251 (MQ=255)                                                           ccGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGc                                                                                                                                                                                           <  1:90661/250‑1 (MQ=255)                                                                aCCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTaa                                                                                                                                                                                     <  1:622314/251‑1 (MQ=255)                                                                aCCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTaa                                                                                                                                                                                     <  1:430643/251‑1 (MQ=255)                                                                       atatTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGaa                                                                                                                                                                                                                           <  1:525216/206‑1 (MQ=255)                                                                         atTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGc                                                                                                                                                                            <  1:144022/251‑1 (MQ=255)                                                                                      aTGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACt                                                                                                                                                               <  1:815562/251‑1 (MQ=255)                                                                                                           aTTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      <  1:24144/159‑1 (MQ=255)                                                                                                               aCGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAACTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACg                                                                                                                                      <  1:554465/251‑1 (MQ=255)                                                                                                                cGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATaa                                                                                                                                                                                                                <  1:215985/176‑1 (MQ=255)                                                                                                                    aCGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTaa                                                                                                                                                                                     >  1:398228/1‑199 (MQ=255)                                                                                                                    aCGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTaa                                                                                                                                 >  1:675749/1‑251 (MQ=255)                                                                                                                                gCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      >  1:539783/1‑138 (MQ=255)                                                                                                                                               ccATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGAcc                                                                                                                                                                                                           >  1:588443/1‑150 (MQ=255)                                                                                                                                               ccATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGtttt                                                                                                      >  1:558885/1‑251 (MQ=255)                                                                                                                                                  ttCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      >  1:544403/1‑120 (MQ=255)                                                                                                                                                       gTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTg                                                                                              >  1:253843/1‑251 (MQ=255)                                                                                                                                                            aGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      >  1:743467/1‑110 (MQ=255)                                                                                                                                                              gTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATc                                                                                                                                                                                   >  1:149357/1‑159 (MQ=255)                                                                                                                                                              gTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTCAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCca                                                                                        >  1:587287/1‑250 (MQ=255)                                                                                                                                                                      ggTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAg                                                                                                                              >  1:809923/1‑204 (MQ=255)                                                                                                                                                                           aTTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTaaa                                                                          >  1:350528/1‑251 (MQ=255)                                                                                                                                                                                    aaaTATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGaaaa                                                                 >  1:704621/1‑251 (MQ=255)                                                                                                                                                                                      atatAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATg                                                                                                                                                                                                                                      <  1:652224/84‑1 (MQ=255)                                                                                                                                                                                      atatAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAAt                                                                                                                                                                                >  1:484347/1‑138 (MQ=255)                                                                                                                                                                                      atatAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGaaaaaa                                                               >  1:205453/1‑251 (MQ=255)                                                                                                                                                                                       tataAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGaaaaaaa                                                              >  1:311089/1‑251 (MQ=255)                                                                                                                                                                                        ataAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCAc                                                                                                                                            <  1:557937/172‑1 (MQ=255)                                                                                                                                                                                                        cgcacTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTc                                             <  1:808848/251‑1 (MQ=255)                                                                                                                                                                                                          cacTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAAc                                                                        <  1:11580/222‑1 (MQ=255)                                                                                                                                                                                                            cTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTaaaa                                                                                                                                                      >  1:398099/1‑142 (MQ=255)                                                                                                                                                                                                            cTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAg                                         >  1:464540/1‑251 (MQ=255)                                                                                                                                                                                                              ccGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAg                                                                                                                              >  1:73481/1‑164 (MQ=255)                                                                                                                                                                                                                      tttttCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTc                                             >  1:494578/1‑237 (MQ=255)                                                                                                                                                                                                                          tCGCGCGAAATTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAAt                           <  1:292702/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaTTCGGAAATTCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGct                     <  1:608954/250‑1 (MQ=255)                                                                                                                                                                                                                                             tCAAAAAGTGTCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGCTCCTCCTGCTGGAc        <  1:89310/251‑1 (MQ=255)                                                                                                                                                                                                                                                    gtgtCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGCTCCTCCTGCTGGACTTGAAc  >  1:285510/1‑250 (MQ=255)                                                                                                                                                                                                                                                    gtgtCAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGCTCCTCCTGCTGGACTTGAAc  >  1:420564/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CAACAGGATTTGGTCAGCAAAGGCTTCCGTATCGGCATGGCGAACAATTCCAATGCTGCCGTCACCGTAGATATTCAGCAATTTTATGCCAAAGTGAATCAAGGCAATTTACGCTACGACATTGACAGCAAAATTCAGCTTGCCATTCATGTTCAAGGTCGCAGAGGTCAATTCACGAAAAATATAAATGCCGGGCGCACGCACTCCGGTGCATTTTTCGCGCGAAATTCGGAAATTCAAAAAGTGTTAGGCGAAACCTTTAATGAAGTGGTGGAATCAATTTATAAAGACCAGGAAGTTGCTAACGCCATTAATCAATACGCCAACTAAAAAACTTTTCTAAAATCCACCGCACTTTACGCTTAAAAGTGCGGTAGATTTTTCCGGTGTTTTTTAATTTGCCCCCACACAAAAGCGTAAAACCGGAAAAAAACAAAAGCCGCTAATTTCTTAGCGGCTTTCTCGAATTTGGCTCCTCCTGCTGGACTTGAAC  >  1/1602063‑1602555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑