Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,595,349 C→T V361V (GTG→GTA)  unknown ← Na(+)‑translocating NADH‑quinone reductase subunit F (EC 1.6.5.8)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,595,3490CT100.0% 211.7 / NA 79V361V (GTG→GTA) unknownNa(+)‑translocating NADH‑quinone reductase subunit F (EC 1.6.5.8)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/41);  total (38/41)

TGATGAATCAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGCACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGCACCACCACCGA  >  1/1595106‑1595587
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
tgatgaATCAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATtg                                                                                                                                                                                                                                          <  1:901261/250‑1 (MQ=255)
     aaTCAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAAt                                                                                                                                                                                                                                     <  1:846939/250‑1 (MQ=255)
     aaTCAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAAt                                                                                                                                                                                                                                     <  1:548212/250‑1 (MQ=255)
        cAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAg                                                                                                                                                                                                                                 <  1:333132/251‑1 (MQ=255)
         aaTATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAg                                                                                                                                                                                                                                 >  1:743263/1‑250 (MQ=255)
         aaTATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGc                                                                                                                                                                                                                                <  1:204117/251‑1 (MQ=255)
                   aCGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGcc                                                                                                                                                                                                                      <  1:252203/251‑1 (MQ=255)
                   aCGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGcc                                                                                                                                                                                                                      <  1:694591/251‑1 (MQ=255)
                         tAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTcc                                                                                                                                                                                                                 <  1:521391/250‑1 (MQ=255)
                                                   gTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATtg                                                                                                                                                                                                                                          >  1:505851/1‑199 (MQ=255)
                                                    tGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGAt                                                                                                                                                                                       <  1:829845/249‑1 (MQ=255)
                                                    tGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGAt                                                                                                                                                                                       <  1:268741/249‑1 (MQ=255)
                                                             tttttACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATg                                                                                                                                                                            >  1:560404/1‑251 (MQ=255)
                                                               tttACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGcc                                                                                                                                                                                                                               <  1:141115/198‑1 (MQ=255)
                                                                       gtTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGAt                                                                                                                                                                                       >  1:666275/1‑230 (MQ=255)
                                                                       gtTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGaaa                                                                                                                                                                  >  1:243762/1‑251 (MQ=255)
                                                                                cTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGAttt                                                                                                                                                         >  1:65654/1‑251 (MQ=255)
                                                                                       gCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTTTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAATTTCGGATTTTCCGCtt                                                                                                                                                  >  1:509089/1‑251 (MQ=255)
                                                                                       gCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCtt                                                                                                                                                  >  1:520777/1‑251 (MQ=255)
                                                                                       gCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCtt                                                                                                                                                  >  1:209506/1‑251 (MQ=255)
                                                                                                   gAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCaa                                                                                                                                      >  1:553981/1‑251 (MQ=255)
                                                                                                             aaCAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGc                                                                                                                                                                                                                                >  1:458142/1‑151 (MQ=255)
                                                                                                             aaCAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTa                                                                                                                             >  1:768974/1‑250 (MQ=255)
                                                                                                               cAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          <  1:155552/251‑1 (MQ=255)
                                                                                                               cAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          <  1:654447/251‑1 (MQ=255)
                                                                                                               cAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          <  1:56253/251‑1 (MQ=255)
                                                                                                                 aGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCgg                                                                                                                                                                                         >  1:2082/1‑186 (MQ=255)
                                                                                                                   atatTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAg                                                                                                                        <  1:510808/249‑1 (MQ=255)
                                                                                                                    tatTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAAc                                                                                                                     <  1:628797/251‑1 (MQ=255)
                                                                                                                     atTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATtgt                                                                                                                                                                                                                                         <  1:612411/134‑1 (MQ=255)
                                                                                                                      ttttCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAg                                                                                                                                                                                                                                 >  1:827075/1‑141 (MQ=255)
                                                                                                                             cTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGcc                                                                                                                                                                                                                      >  1:873396/1‑145 (MQ=255)
                                                                                                                             cTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGAt                                                                                                                                                                                       <  1:111320/176‑1 (MQ=255)
                                                                                                                              ttCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTTCTCATGCAATGGATCGGATACTGCCACACGCCATTGGAAATTCGGACTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACAtttacagtt                                                                                                           >  1:862559/1‑245 (MQ=255)
                                                                                                                              ttCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCaaaa                                                                                                                                    >  1:183538/1‑226 (MQ=255)
                                                                                                                                   cacCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          >  1:346271/1‑231 (MQ=255)
                                                                                                                                   cacCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGaa                                                                                                      <  1:275559/251‑1 (MQ=255)
                                                                                                                                          gTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATtg                                                                                                                                                                                                                                          <  1:450825/112‑1 (MQ=255)
                                                                                                                                          gTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAAt                                                                                                                                   <  1:213339/215‑1 (MQ=255)
                                                                                                                                               tCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGAt                                                                                                                                                                                            >  1:876998/1‑153 (MQ=255)
                                                                                                                                                  aCATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAg                                                                                       <  1:332219/251‑1 (MQ=255)
                                                                                                                                                    aTTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAAtc                                                                                                                                  <  1:286725/206‑1 (MQ=255)
                                                                                                                                                    aTTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGaa                                                                                     <  1:805392/251‑1 (MQ=255)
                                                                                                                                                     ttttGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAg                                                                                    <  1:236126/251‑1 (MQ=255)
                                                                                                                                                     ttttGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAg                                                                                    <  1:199574/251‑1 (MQ=255)
                                                                                                                                                           ttACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAg                                                                                    <  1:175359/245‑1 (MQ=255)
                                                                                                                                                                 cAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACg                                                                         <  1:150801/250‑1 (MQ=255)
                                                                                                                                                                     gTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTg                                                                                                                                                                                                            >  1:766328/1‑115 (MQ=255)
                                                                                                                                                                     gTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAAc                                                                                                                     <  1:140625/202‑1 (MQ=255)
                                                                                                                                                                     gTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTag                                                                    >  1:562013/1‑251 (MQ=255)
                                                                                                                                                                     gTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCACTTGCAATGTATCAAAATCTTCTACATAGa                                                                                                                       >  1:453894/1‑200 (MQ=255)
                                                                                                                                                                          tcaCCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTagag                                                                  <  1:372914/248‑1 (MQ=255)
                                                                                                                                                                          tcaCCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAgtgt                                                               >  1:745257/1‑251 (MQ=255)
                                                                                                                                                                             ccGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAGCGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTaaa                                                            >  1:101991/1‑251 (MQ=255)
                                                                                                                                                                                  ggTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGttt                                                       >  1:85775/1‑251 (MQ=255)
                                                                                                                                                                                      cGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGGGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAAt                                                   <  1:449008/251‑1 (MQ=255)
                                                                                                                                                                                        cacaTGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATaa                                                                                                                                                                                     >  1:530062/1‑119 (MQ=255)
                                                                                                                                                                                        cacaTGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTg                                                 >  1:570395/1‑251 (MQ=255)
                                                                                                                                                                                          caTGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAg                                                                                       >  1:202915/1‑211 (MQ=255)
                                                                                                                                                                                          caTGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACg                                                          <  1:45070/240‑1 (MQ=255)
                                                                                                                                                                                          caTGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAAt                                                   >  1:390865/1‑247 (MQ=255)
                                                                                                                                                                                             gtagtaTTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          <  1:853434/173‑1 (MQ=255)
                                                                                                                                                                                             gtagtaTTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGa                                            >  1:832647/1‑251 (MQ=255)
                                                                                                                                                                                                gtaTTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          >  1:808226/1‑170 (MQ=255)
                                                                                                                                                                                                  aTTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACa                                                                                                                           <  1:422520/167‑1 (MQ=255)
                                                                                                                                                                                                  aTTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTAc                                                                          >  1:670026/1‑216 (MQ=255)
                                                                                                                                                                                                    tCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGAtgtg                                     >  1:599637/1‑251 (MQ=255)
                                                                                                                                                                                                      acaATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGAtgtgtg                                   >  1:709227/1‑251 (MQ=255)
                                                                                                                                                                                                         aTCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACAt                                                                                                                          >  1:899138/1‑161 (MQ=255)
                                                                                                                                                                                                         aTCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTagag                                                                  <  1:723406/217‑1 (MQ=255)
                                                                                                                                                                                                         aTCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAAc                                <  1:841345/251‑1 (MQ=255)
                                                                                                                                                                                                                       cGTGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATa                  <  1:44896/251‑1 (MQ=255)
                                                                                                                                                                                                                         tGATTTTTCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATAcc                <  1:676563/251‑1 (MQ=255)
                                                                                                                                                                                                                               ttCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAgg                                                                                   >  1:280180/1‑178 (MQ=255)
                                                                                                                                                                                                                               ttCAGATAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGCAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATAcc                >  1:202546/1‑245 (MQ=255)
                                                                                                                                                                                                                                    aTAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGcaccacc       <  1:370259/249‑1 (MQ=255)
                                                                                                                                                                                                                                    aTAGTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGcaccac        <  1:122123/248‑1 (MQ=255)
                                                                                                                                                                                                                                       gTTTTCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGCACCACCACCGa  >  1:833852/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ttttCATAAAGTACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGCACCACCACCg   <  1:745693/249‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
TGATGAATCAATATGAAAAACGCTGTAAATTTCGACCGCACTTCATGCAAAGTGCGGTCATTTTTTACGGTGTTTTTAAACTGCAAGGCGTTAGCCGCCGAAATCATCTAACAAGATATTTTCATCTTCAACACCAAGGTCTTTCAACATTTTGATTACCGCAGCGTTCATCACCGGAGGTCCGCACATGTAGTATTCACAATCTTCCGGCGCTTCGTGATTTTTCAGATAGTTTTCATAAAGCACATTGTGAATAAAGCCGGTGTAGCCTGTCCAGTTGTCTTCCGGCAATGGATCGGATAATGCCACATGCCATTGGAAATTCGGATTTTCCGCTTGCAATGTATCAAAATCTTCTACATAGAACATTTCACGTTTAGAACGGGCACCGTACCAGAAGGACATTTTACGTTTAGAGTGTAAACGTTTTAATTGGTCGAAGATGTGTGAACGCATTGGTGCCATACCTGCACCACCACCGA  >  1/1595106‑1595587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑