Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,584,254	A→G	H555R (CAC→CGC)	unknown →	Alanyl‑tRNA synthetase (EC 6.1.1.7)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,584,254	0	A	G	100.0%	211.8 / NA	78	H555R (CAC→CGC)	unknown	Alanyl‑tRNA synthetase (EC 6.1.1.7)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/37);  total (41/37)

TTCCATGACGGCAAACCAGTAGATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCACCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATGGAGCTGGA  >  1/1584007‑1584488                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           ttCCATGACGGCAAACCGGTAGATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCg                                                                                                                                                                                                                                         <  1:531807/251‑1 (MQ=255)                ccAGTAGATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCAtt                                                                                                                                                                                                                          >  1:861681/1‑251 (MQ=255)                   gTAGATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTaaa                                                                                                                                                                                                                       >  1:653230/1‑251 (MQ=255)                      gATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATc                                                                                                                                                                                                                     <  1:782127/250‑1 (MQ=255)                        tACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTAtt                                                                                                                                                                                                                               <  1:615302/238‑1 (MQ=255)                           cATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCAct                                                                                                                                                                                                                  >  1:709265/1‑248 (MQ=255)                                gTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAAc                                                                                                                                                                                                           >  1:400959/1‑250 (MQ=255)                                 ttCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAAc                                                                                                                                                                                                           <  1:579059/249‑1 (MQ=255)                                      gTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATttg                                                                                                                                                                                                    >  1:151772/1‑251 (MQ=255)                                      gTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATttg                                                                                                                                                                                                    >  1:665581/1‑251 (MQ=255)                                       tCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATttgt                                                                                                                                                                                                   <  1:167351/251‑1 (MQ=255)                                               cGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCAcgc                                                                                                                                                                                            <  1:49637/250‑1 (MQ=255)                                                        ttttAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGt                                                                                                                                                                                  <  1:598942/251‑1 (MQ=255)                                                         tttAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTc                                                                                                                                                                                 <  1:115394/251‑1 (MQ=255)                                                         tttAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTc                                                                                                                                                                                 <  1:886532/251‑1 (MQ=255)                                                              ataataCGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTc                                                                                                                                                                            <  1:851378/251‑1 (MQ=255)                                                                 ataCGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAAccc                                                                                                                                                                                                         >  1:776528/1‑219 (MQ=255)                                                                   aCGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCAt                                                                                                                                                                                                                           <  1:860765/199‑1 (MQ=255)                                                                   aCGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAgg                                                                                                                                                                       >  1:789910/1‑251 (MQ=255)                                                                        aTTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACAAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGaa                                                                                                                                                                   >  1:423433/1‑250 (MQ=255)                                                                             aTGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGc                                                                                                                                                                      >  1:584542/1‑242 (MQ=255)                                                                                      ccGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGaaa                                                                                                                                                    >  1:289123/1‑251 (MQ=255)                                                                                           ggtCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGAt                                                                                                                                                >  1:705984/1‑250 (MQ=255)                                                                                                aaTCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGGCGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATGAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAgg                                                                                                                                                                       <  1:448916/222‑1 (MQ=255)                                                                                                aaTCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTg                                                                                                                                          >  1:283245/1‑251 (MQ=255)                                                                                                         cATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATg                                                                                                                                 <  1:672323/251‑1 (MQ=255)                                                                                                                  cTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGc                                                                                                                                                                      >  1:808772/1‑205 (MQ=255)                                                                                                                  cTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTg                                                                                                                         <  1:280525/250‑1 (MQ=255)                                                                                                                               ggTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGaaa                                                                                                                                                    >  1:642367/1‑210 (MQ=255)                                                                                                                                gTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTc                                                                                                                                                                                                                             >  1:277124/1‑136 (MQ=255)                                                                                                                                            aCGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAAcc                                                                                               <  1:670862/250‑1 (MQ=255)                                                                                                                                             cGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTa                                                                                             >  1:184452/1‑251 (MQ=255)                                                                                                                                              gTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGt                                                                                                                      >  1:668688/1‑225 (MQ=255)                                                                                                                                               tAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATc                                                                                           <  1:794255/251‑1 (MQ=255)                                                                                                                                                  aaGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGt                                                                                        <  1:125566/251‑1 (MQ=255)                                                                                                                                                  aaGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGt                                                                                        <  1:568863/251‑1 (MQ=255)                                                                                                                                                        cccAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTg                                                                                                                                          <  1:612122/195‑1 (MQ=255)                                                                                                                                                        cccAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGAc                                                                                                                >  1:243394/1‑221 (MQ=255)                                                                                                                                                        cccAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAAcc                                                                                               >  1:56511/1‑238 (MQ=255)                                                                                                                                                        cccAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAgcg                                                                                  >  1:56721/1‑251 (MQ=255)                                                                                                                                                              aaTATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTc                                                                                                                                                                                 >  1:417676/1‑150 (MQ=255)                                                                                                                                                                   ggTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAgg                                                                                                                                                                       >  1:735554/1‑155 (MQ=255)                                                                                                                                                                      cAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGt                                                                    >  1:86271/1‑251 (MQ=255)                                                                                                                                                                        ggTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAAcc                                                                                               <  1:372673/222‑1 (MQ=255)                                                                                                                                                                           aTTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCGCTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAAc                                                               <  1:3267/251‑1 (MQ=255)                                                                                                                                                                           aTTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTaa                                                                                      <  1:776436/228‑1 (MQ=255)                                                                                                                                                                              cGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGaaa                                                                                                                                                    >  1:127353/1‑163 (MQ=255)                                                                                                                                                                                gTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCAt                                                           <  1:491286/250‑1 (MQ=255)                                                                                                                                                                                 tCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAg                                                         <  1:900944/251‑1 (MQ=255)                                                                                                                                                                                    tatCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAgg                                                                                                                                                                       >  1:379030/1‑138 (MQ=255)                                                                                                                                                                                       cGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAAt                                                    >  1:111318/1‑250 (MQ=255)                                                                                                                                                                                       cGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATc                                                   <  1:285674/251‑1 (MQ=255)                                                                                                                                                                                         gTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTg                                                                                                                         >  1:89442/1‑179 (MQ=255)                                                                                                                                                                                           gAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCa                                               >  1:764727/1‑251 (MQ=255)                                                                                                                                                                                            aaCTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGc                                                <  1:891803/249‑1 (MQ=255)                                                                                                                                                                                            aaCTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCa                                               <  1:860752/250‑1 (MQ=255)                                                                                                                                                                                                 aCCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTag                                         >  1:358618/1‑251 (MQ=255)                                                                                                                                                                                                    cAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGc                                      <  1:697653/251‑1 (MQ=255)                                                                                                                                                                                                    cAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGc                                      <  1:774856/251‑1 (MQ=255)                                                                                                                                                                                                      aGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAAc                                                               >  1:262250/1‑224 (MQ=255)                                                                                                                                                                                                      aGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGa                                    >  1:771670/1‑251 (MQ=255)                                                                                                                                                                                                             ttATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAAccc                             <  1:166894/251‑1 (MQ=255)                                                                                                                                                                                                                 ccGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGAtt                         <  1:401422/251‑1 (MQ=255)                                                                                                                                                                                                                   gTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAAcc                                                                                               <  1:61497/179‑1 (MQ=255)                                                                                                                                                                                                                   gTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAgcgc                                                                                 >  1:756668/1‑193 (MQ=255)                                                                                                                                                                                                                   gTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGt                                                        >  1:51015/1‑218 (MQ=255)                                                                                                                                                                                                                   gTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCg                                     >  1:652053/1‑237 (MQ=255)                                                                                                                                                                                                                       ggCAGTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGATCGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAAc                    <  1:38423/250‑1 (MQ=255)                                                                                                                                                                                                                           gTCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAAc                    <  1:140496/246‑1 (MQ=255)                                                                                                                                                                                                                            tCCGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGatat              <  1:239484/251‑1 (MQ=255)                                                                                                                                                                                                                             ccGTGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGAtatt             >  1:294/1‑251 (MQ=255)                                                                                                                                                                                                                                tGAATGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATg          <  1:506216/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGCAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATgg         >  1:70318/1‑249 (MQ=255)                                                                                                                                                                                                                                      cAGTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAAc                                                               <  1:654106/192‑1 (MQ=255)                                                                                                                                                                                                                                        gTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATGGAGCTGGa  >  1:644756/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATGGAGCTGGa  >  1:9693/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTGGTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACCCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATGGAGCTGGa  >  1:206890/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggTTGACGAGGTTCGCCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAAcc                                                                                               >  1:559033/1‑156 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           TTCCATGACGGCAAACCAGTAGATACCATCAGTTCCGGTCAAAGTGCGGTGGTGATTTTAGATAATACGCCATTCTATGCGGAATCCGGTGGTCAAATCGGAGACATCGGGCGCTTAGAAGGCAACGGTTTTAATTTTGACGTAAAAGACACCCAAAAATATGGTCAGGTATTCGGTCATATCGGTGAACTTACCCAAGGTAGTTTATCCGTCGGGCAGTCCGTGAATGCAGTGGTTGACGAGGTTCACCGTCAACGTATTTCATTAAATCACTCTGCAACCCATTTGTTGCACGCCGCGTTACGTCAGGTCTTAGGCGAACACGTCGCTCAGAAAGGATCGCTTGTTTCTGATGCATTATTGCGTTTTGACTTTGCTCAACATGAACCTATCAGTAAAGCGCAATTAGCCGAAGTAGAACGCATAGTTAATCAGCAAGTTAGAGCGAATAACCCGATTAAAACGGATATTATGGAGCTGGA  >  1/1584007‑1584488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑