Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,577,645	A→G	intergenic (‑96/‑31)	unknown ← / → unknown	Nucleoid‑associated protein NdpA/UPF0352 protein YejL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,577,645	0	A	G	100.0%	191.1 / NA	68	intergenic (‑96/‑31)	unknown/unknown	Nucleoid‑associated protein NdpA/UPF0352 protein YejL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/29);  total (39/29)

ACATCATTTGTTCTACTTCCGGCGTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATACGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATACGA  >  1/1577411‑1577889                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     aCATCATTTGTTCTACTTCCGGCGTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCgg                                                                                                                                                                                                                                      >  1:587969/1‑251 (MQ=255)               aCTTCCGGCGTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAAc                                                                                                                                                                                                                        <  1:596894/251‑1 (MQ=255)                cTTCCGGCGTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACa                                                                                                                                                                                                                       >  1:742855/1‑251 (MQ=255)                        gTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAAtt                                                                                                                                                                                                               >  1:846363/1‑251 (MQ=255)                              gTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATtcaatca                                                                                                                                                                                                         >  1:627289/1‑251 (MQ=255)                                cAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCaaa                                                                                                                                                                                                       >  1:791589/1‑251 (MQ=255)                                       tGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCaa                                                                                                                                                                                                >  1:532917/1‑251 (MQ=255)                                       tGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCaa                                                                                                                                                                                                >  1:901611/1‑251 (MQ=255)                                          ttACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGAt                                                                                                                                                                                             <  1:146007/251‑1 (MQ=255)                                                  cACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAAt                                                                                                                                                                                     <  1:366333/251‑1 (MQ=255)                                                      cTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGc                                                                                                                                                                                                                   >  1:164153/1‑217 (MQ=255)                                                      cTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGAt                                                                                                                                                                                             <  1:39383/239‑1 (MQ=255)                                                      cTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGAt                                                                                                                                                                                 >  1:255055/1‑251 (MQ=255)                                                        aTTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGaccaacca                                                                                                                                                                                                                           >  1:315761/1‑207 (MQ=255)                                                        aTTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGc                                                                                                                                                                                                                   >  1:890397/1‑215 (MQ=255)                                                        aTTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGATTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGa                                                                                                                                                                               >  1:444502/1‑251 (MQ=255)                                                                    caCGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAAc                                                                                                                                                                     <  1:260848/249‑1 (MQ=255)                                                                               gttcggttTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAAc                                                                                                                                                                                                                        >  1:27400/1‑187 (MQ=255)                                                                                ttcggttTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAg                                                                                                                                                        <  1:36084/250‑1 (MQ=255)                                                                                ttcggttTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCACAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAg                                                                                                                                                        <  1:398899/250‑1 (MQ=255)                                                                                       tGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGaa                                                                                                                                                <  1:524440/251‑1 (MQ=255)                                                                                             gATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAaca                                                                                                                                           >  1:623941/1‑250 (MQ=255)                                                                                                   aTAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAgcg                                                                                                                                    >  1:54898/1‑251 (MQ=255)                                                                                                                gaATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAAt                                                                                                                                                                                                                >  1:725637/1‑162 (MQ=255)                                                                                                                 aataatTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATc                                                                                                                      <  1:185749/251‑1 (MQ=255)                                                                                                                       ttGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAAt                                                                                                                <  1:204454/251‑1 (MQ=255)                                                                                                                               cTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAg                                                                                                        >  1:331847/1‑251 (MQ=255)                                                                                                                                 gTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAg                                                                                                                                                                                               >  1:408841/1‑162 (MQ=255)                                                                                                                                 gTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAgg                                                                                                       >  1:387643/1‑250 (MQ=255)                                                                                                                                 gTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAgg                                                                                                       >  1:647318/1‑250 (MQ=255)                                                                                                                                       cTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAatat                                                                                                 <  1:408569/250‑1 (MQ=255)                                                                                                                                         cATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGa                                                                                               >  1:764403/1‑250 (MQ=255)                                                                                                                                                    ccTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTAc                                                                                   <  1:271229/251‑1 (MQ=255)                                                                                                                                                    ccTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTAc                                                                                   <  1:465950/251‑1 (MQ=255)                                                                                                                                                      ttAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAAt                                                                                                                                                                                     <  1:143376/151‑1 (MQ=255)                                                                                                                                                      ttAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAg                                                                                                                                                        >  1:46395/1‑180 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACa                                                                                                                                                                                        <  1:818784/136‑1 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATtctc                                                                                                                                                                        >  1:296919/1‑152 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGAt                                                                                                                                                             <  1:542016/163‑1 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTa                                                                                                         >  1:143600/1‑215 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGc                                                                                <  1:259537/240‑1 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAgtgt                                                                      >  1:837167/1‑250 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAgtgt                                                                      >  1:288483/1‑250 (MQ=255)                                                                                                                                                                  gTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACAACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAgtgt                                                                      >  1:254034/1‑250 (MQ=255)                                                                                                                                                                      attatAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGc                                                                                                                                                          <  1:586633/162‑1 (MQ=255)                                                                                                                                                                       ttatAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGAt                                                                                                                                                                                 <  1:379860/138‑1 (MQ=255)                                                                                                                                                                                  cTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGa                                                      >  1:825337/1‑250 (MQ=255)                                                                                                                                                                                  cTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAACGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGa                                                      >  1:427866/1‑250 (MQ=255)                                                                                                                                                                                             aaCTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCa                                          <  1:506865/251‑1 (MQ=255)                                                                                                                                                                                                         atGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATc                                                                                                                      >  1:857988/1‑163 (MQ=255)                                                                                                                                                                                                         atGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTtctc                               >  1:857244/1‑250 (MQ=255)                                                                                                                                                                                                         atGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTcc                              <  1:531007/251‑1 (MQ=255)                                                                                                                                                                                                          tGTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCg                             >  1:770313/1‑251 (MQ=255)                                                                                                                                                                                                           gTTAGGATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCt                                                                                                                    <  1:793737/163‑1 (MQ=255)                                                                                                                                                                                                                gATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATg                                                                                                >  1:94489/1‑178 (MQ=255)                                                                                                                                                                                                                gATTAGCGCAATTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCa                                          <  1:196255/232‑1 (MQ=255)                                                                                                                                                                                                                          aTTATCGGTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAatat                                                                                                                                                                                                   >  1:510743/1‑69 (MQ=255)                                                                                                                                                                                                                                 gTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACa                                                                                                                                                                                        <  1:891316/73‑1 (MQ=255)                                                                                                                                                                                                                                 gTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAAt      >  1:235179/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTTAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAAt      >  1:631875/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATa     <  1:261346/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATa     <  1:410480/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATa     <  1:199037/251‑1 (MQ=255)                                                                                                                                                                                                                                   tAATATATGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCTCCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATac    <  1:581443/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaTATATGCGGCATTTTAAATCGGCAGACCAACCAAAc                                                                                                                                                                                                                        >  1:187969/1‑38 (MQ=255)                                                                                                                                                                                                                                     atatatGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGc                                                                                                                                                          <  1:486047/99‑1 (MQ=255)                                                                                                                                                                                                                                     atatatGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATacga  >  1:281796/1‑251 (MQ=255)                                                                                                                                                                                                                                     atatatGCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATacga  >  1:872084/1‑251 (MQ=255)                                                                                                                                                                                                                                      tatatgCGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATcc                                                                                                                     >  1:763053/1‑135 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     ACATCATTTGTTCTACTTCCGGCGTGATTGTCAGTACTTGTTTACGTAGCACGCTATTCAGTTGCAGCACGCTTTTCGGTTCGGTTTGATTGGATTTGATAATTTGGTGGAGAATAATTTGTTTAACTGTAATGCTCATGAAATTGTCCTTAAAAAACTCTGTCTATTATAACCGCACTTTTTACCGGAACTAACAAAATATGTTAGGATTAGCGCAATTATCGGTTAATATATACGGCATTTTAAATCGGCAGACCAACCAAACATGGCAATTCAATCAAAATATCAAGATAAACAAATAGATGAAATTCTCAACGACATGATTGCAGTGCTGGAAAAACACCAAGCGCCGCTGGATTTATCCTTAATCGTGTTAGGCAATATGACCACCAATTTACTGCTCGGCAGTGTCGGAAAACAGCAACGACAAGTGCTGGCAAAAGCCTTCTCCGACGCTTTGCTGAACTCTCTTAATACGA  >  1/1577411‑1577889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑